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Top : New Forum Archives (2009-): : Molecular-Biology
2581. PCR issue - (reply: 2)
2582. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
2583. Synthetic intron pIRES needed - (reply: 2)
2584. Purfiying Unmethylated Plasmid - unmethylated plasmid (reply: 3)
2585. cDNA question - (reply: 3)
2586. PCR - (reply: 1)
2587. Freeze thawing RNA - (reply: 4)
2588. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
2589. Precipitation in NaOH on shelf - (reply: 3)
2590. long PCR primer - (reply: 7)
2591. stable 3t3l1 transfection:serious help needed!!! - (reply: 10)
2592. methods to detect EGFP expression ?! - we have a big problem! (reply: 10)
2593. mutation study - DNA or mRNA sequence (reply: 1)
2594. how to remove a restricted endonuclease site - (reply: 3)
2595. substractive hybridization - (reply: 2)
2596. sequencing problem after pcr - (reply: 4)
2597. Amplifying plasmid - non specific binding of primer - (reply: 3)
2598. DNA folding to form the loop - (reply: 2)
2599. primer design - (reply: 3)
2600. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
2601. DNA purification - long fragments wanted - (reply: 1)
2602. want gene knock out protocol - (reply: 2)
2603. Unidentified DNA bands - (reply: 1)
2604. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
2605. Accidentally digested DNA overnight - still OK? - (reply: 4)
2606. Shelf life of ampicillin in LB media? - (reply: 6)
2607. Make construct so it is only thing that can PCR after transfection - (reply: 2)
2608. Negative flouroscence in real time PCR - (reply: 2)
2609. Remove RNA after DNA mini-prep - (reply: 1)
2610. Minimum number of PCR cycles to see a product? - (reply: 6)