Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Biology
2581. Transfection efficiency vs. amount of plasmid DNA - (reply: 1)
2582. pIRESneo for mammalian cells transfection - (reply: 4)
2583. H9C2 - cell line - Need clarification (reply: 1)
2584. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
2585. no band in gel - gel electrophoresis (reply: 1)
2586. stratagene site directed mutagenesis - deletion of single base nucleotide (reply: 3)
2587. How to increase A260/280 and A260/230 ratio for microarray experiment? - (reply: 8)
2588. western blot stripping buffer - please recomend a stripping buffer for re-bloting (reply: 2)
2589. T7 and T7lac - (reply: 1)
2590. Sources of Contamination in plasmid DNA Mini-Preps - (reply: 10)
2591. Primer stock confusion - (reply: 6)
2592. Pancreas RNA extraction - improve protocol needed (reply: 2)
2593. Genomic DNA contamination in RNA sample - (reply: 2)
2594. Isolation DNA and protein from a single sample - (reply: 5)
2595. lipofectamine - (reply: 3)
2596. Gel Analysis Software - which to use (reply: 3)
2597. DNase I digestion - check if it worked?? (reply: 4)
2598. Uniform high backgroud in DIG Sounthern Blot - (reply: 2)
2599. elution of dna from gel - (reply: 5)
2600. What is in the Blocking solution for DIG-Southern Blot? - (reply: 2)
2601. In vitro Transcription kit recommendations? - (reply: 5)
2602. GFP reporter gene - (reply: 3)
2603. Possible to lose a pGEM insert ? - (reply: 2)
2604. PROTEIN PRECIPITATION SOLUTION - (reply: 1)
2605. cloning - How to calculate the insert and linear plsmid concentration for ligati (reply: 1)
2606. PFGE gel - Can I reuse pulsed field certified gel for PFGE? (reply: 2)
2607. Validating real time pcr primers - (reply: 2)
2608. How to find catalytic domain using cDNA - (reply: 2)
2609. low plasmid yield from midi preps - (reply: 4)
2610. Measure nucleotides - (reply: 3)