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Medium Related Discussions
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Concentrating Conditioned Media for use on a non-denaturing agarose gel - (reply: 2)
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Making media with lots of supplements - How to add transferrin? (reply: 1)
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Differences in absorbancy of compound in different media - (reply: 5)
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How to change medium without dislodging the cells - (reply: 1)
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glycerol in TB medium - (reply: 1)
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protein purification from media supernatant? - protein purification of ECM proteins (reply: 2)
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Changing Media for Lentiviral Infection in a Suspension Culture - (reply: 1)
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how long does media with serum last at 4 deg? - (reply: 4)
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How do you guys prepare 10% FBS Medium? - (reply: 3)
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Technique to estimate the level of protein expression from media - (reply: 2)
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2% ethanol in medium, can shsy5y stand it ? - (reply: 3)
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can you freeze media with antibiotics in it - (reply: 1)
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inoculating liquid media from frozen cell stock ok? - (reply: 6)
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Troubleshooting! Strange growth in LB medium - (reply: 3)
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Analysing media - (reply: 2)
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Minimal media question - Minimal media for B.subtilis and B.cereus (reply: 1)
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Dendritic cell growth medium - (reply: 3)
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Nutrient deficient medium - (reply: 1)
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L-glut in media? - (reply: 6)
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How to retain water in the agar medium when culturing in high temperature - (reply: 7)
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Media Expiry - (reply: 3)
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Defined medium preparation - Any guidelines to adhere to? (reply: 1)
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Cell culture supernatant - Is it just the media from cultured cells? (reply: 1)
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Different Brands of Media - (reply: 5)
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resazurin and cysteine in the media for anaerobic bacteria - basic question (reply: 1)
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BL21 and LB media - (reply: 3)
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what is difference of E.coli grow on different media? - (reply: 4)
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adaptation to different medium? - (reply: 1)
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cell cultur medium - (reply: 3)
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What's the difference between KH2PO4 and K2HPO4 in MOPS media? - (reply: 3)
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Filamentous structure observed in E.coli LB medium - (reply: 1)
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Culture media with human serum gives false positive - (reply: 1)
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Medium for culturing 2 types of cell together - (reply: 2)
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isolate yeast - i want to isolate yeast using gye medium but other fungi dominate the (reply: 1)
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Plating cells in Serum-free medium - How to plate Mcf7 cells in a serum free medium? (reply: 7)
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serum and Akt phosphorylation - Do I have use serum-free media to check pAkt when western blotting? (reply: 4)
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Selective media question - (reply: 2)
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Freezing medium - (reply: 5)
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Propagation of breast cancer cell lines in serum free media? - (reply: 1)
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How much media needed for cells in different cell culture plates - (reply: 3)
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crystal violet adhesion assay and serum - should serum in media be excluded (reply: 3)
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HEP G2 cells - growth medium - (reply: 2)
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CHO cells medium - (reply: 5)
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problem using IPL 41 medium - (reply: 3)
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Autoclaving Media Versus Sterile Filter - (reply: 19)
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LCM embedding medium - (reply: 1)
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Help determining media composition (e.coli, staph sp.) - (reply: 1)
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Confused about what media to use... - general media or minimal media foe shewanella oneidensis MR1.. (reply: 1)
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what is the media for HUVEC cells? - (reply: 4)
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media or cell problem? - (reply: 5)
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media and transfection - change? (reply: 4)
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Recipe for ECPM1 media? - (reply: 1)
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Help in Clone Expression - Trasformants grow on plate but not in medium (reply: 2)
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Non-growth of E. coli TOP10F' in LA+tetracicliyn media - (reply: 2)
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Western blotting conditioned media problem - Media will not separate when run on gel (reply: 2)
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Good mounting medium - (reply: 4)
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lots of colonies on media with ampiciline - (reply: 3)
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Yeast Contamination - Serum vs. Serum Free Media - (reply: 3)
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media composition - (reply: 6)
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Ion exchange media help - TEAE-cellulose & Dowex 1 (reply: 2)
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Medium for electroporating HEK 293 T-cells - (reply: 2)
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Media turns Turbid after 3 days of culture - (reply: 6)
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A CO2 independent medium limit the range of cell types that can be investigated? - (reply: 1)
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Minimal Glucose media colour - autoclave surprises . . . (reply: 6)
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Help with SOC medium contamination - (reply: 7)
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making media and autoclaving - (reply: 2)
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How to prepare conditioned media? - Do I have to omit supplements for the culture media? (reply: 2)
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SD media + Calcium - (reply: 1)
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Keeping IFN in cellular media - (reply: 2)
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Medium PH - (reply: 7)
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Cat No of M199 medium for endothelial cells URGENT - (reply: 1)
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Stupid mistake when preparing medium - (reply: 22)
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Thiamine & pyridoxine HCL in MS medium - (reply: 1)
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Which media to choose to cultivate bacteria - (reply: 2)
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color of the medium changes following N-acetyl Cysteine (NAC) addition - (reply: 3)
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Conditioned media, filter or centrifuge? - which way is the best way to prep for ELISA (reply: 4)
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Sodium Orthovanadate kinetics in media incubations - (reply: 3)
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collect secreted proteins in cell culture media - (reply: 2)
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E. coli growing in M9 minimal media - (reply: 2)
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Sodium pyruvate in ES cell culture media - (reply: 2)
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Isolation of Probiotic bacteria from yoghurt - which is the best medium?? (reply: 2)
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Control protein in culture medium for Western? - (reply: 3)
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secreted proteins purification - how to concentrate a secreted protein contained in conditioned medium? (reply: 4)
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media composition for smooth muscle cells - starvation and growth media (reply: 2)
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Antibiotics in Cryoprotectant medium? - (reply: 3)
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change medium of HepG2 - (reply: 2)
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Do you really need to adjust pH of medium prepared from powder? - (reply: 5)
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high glucose in media - (reply: 4)
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please help in Anthurium & Orchid Tissue culture - tissue culture medium (reply: 2)
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excess penicillin and streptomycin concentration in culture medium? - (reply: 4)
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how to maintain cell in serum free medium - (reply: 6)
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Immunoprecipitation of culture medium - (reply: 4)
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cell starvation - which medium is good? (reply: 4)
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De fossard et. Al. (1974) culturing media composition - (reply: 1)
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Loss of RNAi after changing culture media? - (reply: 2)
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Protein Extraction from cell media for western blot - (reply: 3)
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Why is an antibiotic free media necessary before transfection? - (reply: 4)
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cell culture with serum media and with serum free media and doubts in luciferase - (reply: 1)
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M9 minimal media - its not working! - (reply: 2)
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Autoclave technique – loss of medium! - (reply: 6)
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Can cell culture media be frozen at -20°C? - (reply: 6)
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E.coli are growing in LB/Amp plates but not in liquid medium - (reply: 6)
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Can cell culture media be frozen at -20°C - (reply: 4)
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medium -preparation for CD34 cells - (reply: 2)
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calculation problem in media preparation - media preparation (reply: 2)
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media for lipase production by submerged fermentation - (reply: 1)
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What is the different between Eeuwens Y3 medium and MS medium - (reply: 1)
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HEK en HeLa medium - (reply: 3)
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B27/Neurobasal medium for hippocampal neuron culture - (reply: 7)
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mouse fibroblast culture: what medium? - (reply: 7)
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Media for HEK293 - (reply: 2)
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Removal of LPS from media? - (reply: 3)
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what type of growth media - (reply: 2)
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cell dead after changing the medium to PBS buffer - (reply: 3)
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3T3-L1 Differentiation Media Preparation Help - (reply: 14)
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Medium for Candida albicans - (reply: 1)
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how important of Serum proportion of culture medium in cell culture - SOS (reply: 8)
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Culture medium for MDA-MB-231 / MCF-7 - which media is good enough? (reply: 6)
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What is the best way to get conditioned medium? - (reply: 2)
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media - limitations and uses of different types of media (reply: 1)
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How to purify protein secreted in medium - (reply: 2)
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Dendritic cell line media - Dendritic cell line media (reply: 1)
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cells and media - (reply: 1)
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what does GC stands for in GC medium Base - (reply: 1)
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How to maintain pH of designed culture media? - Autoclaving, metabolism of cells cause changes in pH (reply: 2)
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Need BSA in Starvation Media? - (reply: 1)
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Low conductivity media for yeast and E.coli - (reply: 6)
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why my BSA contained medium cannot nourish my cells - (reply: 13)
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Adherent HEK293 serum-free protein-free medium - (reply: 4)
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HEK293 Medium, What pH? - (reply: 4)
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Remove BSA from cell culture medium - (reply: 5)
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avicel medium for RNA extraction - crushing of avicel (reply: 1)
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Apply tranfection reagents DIRECTLY onto the cells. - After removing the medium. (reply: 4)
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If I added 2% PS in media - (reply: 2)
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Yeast Stress in Media to increase ubiqutin conjugates - (reply: 1)
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Medium preparation L-15 for MDA-MB-231 - (reply: 3)
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storage of media with antibiotics at 4C.... - (reply: 7)
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selection of culture medium - (reply: 1)
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medium for competent cell - SOB vs SOC (reply: 6)
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antibody purification - how to purify from serum free medium (reply: 1)
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what media shall I use for mycobacterium? - sulfur lacking? (reply: 4)
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WB with cell culture medium - (reply: 2)
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Autoclaving media twice - (reply: 6)
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serum free cell culture medium - (reply: 6)
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media preparation - wakimoto medium (reply: 2)
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The growth behaviour of the transfected cells in hygromycin selection media - (reply: 1)
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Protocol for protein isolation from culture media - (reply: 3)
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Autoclaving liquid media - (reply: 3)
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Mycoplasma PCR detection in media/FBS - (reply: 6)
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Which medium do you use to grow HEK293 cells? - (reply: 7)
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Yeast 2-Hybrid selection media - (reply: 2)
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Preparing NZY medium and growing E. coli - (reply: 2)
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Overlay cell culture medium with oil against evaporation - which oil? (reply: 2)
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geneticin in RPMI medium, color change to yellow - (reply: 3)
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CHO culture: what media and additives? - for stable Fc fusion protein expression (reply: 4)
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Rich Media - (reply: 1)
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Tetracycline in cell culture media - (reply: 5)
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Medium for adherent cells and serum/protein free - (reply: 2)
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Secreted protein degraded in conditioned medium? - (reply: 1)
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How to concentrate conditioned medium? - (reply: 8)
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phenol/chloroform extraction.... - protocols for large/medium scale (reply: 5)
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What's difference between RPMI and DMEM - which media I should use to culture cells after transfection? (reply: 3)
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Urgent!About the seawater (K medium) handling - (reply: 2)
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Phosphate Free RPMI 1640 medium - (reply: 2)
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growing up plant seedlings in MS media - (reply: 7)
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The shelf life of IMEM medium - (reply: 2)
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Questions about serum's role in medium - (reply: 3)
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how to remove media components from protein solutions - (reply: 4)
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Differential media neuronal culture - (reply: 1)
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Triglycerides in culture medium - (reply: 2)
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Medium Richer than TB (Teriffic Broth) ? - (reply: 4)
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Anyone know wat is BMG and BMM media for? - (reply: 2)
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autoclave and media question - (reply: 1)
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Aspergillus niger culture media - (reply: 3)
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HUVEC culturing medium selection - (reply: 4)
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Stability of Tetracycline Media - Stability of Tetracycline Media (reply: 2)
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adding antibiotics to cho cell media - (reply: 2)
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Concentrating conditioned media - (reply: 1)
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Mold plus bleach makes medium turn purple. - (reply: 4)
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PBS wash in between media change - (reply: 9)
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Conflicting opinions - To change media or not to change media (reply: 4)
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Bordetella medium - (reply: 1)
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How to deplete calcium from cell medium? - (reply: 1)
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What is the best way to transfect MEFs? - and what medium do you use (reply: 7)
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geneticin add to RPMI medium, medium change color - (reply: 4)
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macrophage cell line - problem with the media conditions (reply: 5)
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medium must be changed after Calcium transfection? - (reply: 4)
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precipitate coming out of media! - (reply: 12)
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How do i adjust pH of a media - (reply: 4)
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Is it good to replace SOC medium by LB? - (reply: 9)
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Is it necessary to pre-warm medium? - (reply: 13)
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yeast two hybrid, yeast can't grow in liquid medium - (reply: 2)
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how much FBS add into the medium for CaCO2 - (reply: 2)
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Cells Detach With Serum Free Media - (reply: 5)
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questions about bacteria culture medium - (reply: 1)
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why SOC medium is better? - (reply: 1)
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Color of TCBS media plate - (reply: 2)
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starvation medium recipe for Hela cells - (reply: 2)
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Switching CHO cells media from high to low glucose - how would that effect cells? (reply: 1)
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About protein extracted from liquid medium - (reply: 1)
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Western Blots on media - (reply: 4)
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conditioned media for western - (reply: 1)
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A few questions about autoclaving culture medium - (reply: 11)
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Low yield at His tagged-peptide production in labeling media - (reply: 1)
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Why we should not autoclave / sterilize Antibiotic - Fermentation media preparation (reply: 4)
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puromycin in culture medium - (reply: 3)
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Can adding cold medium (4 degree) synchronize cells? - (reply: 2)
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What is serum-free media? - (reply: 6)
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selective media for xanthomonas sp - (reply: 2)
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Question about medium change during transfection - (reply: 1)
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media for revival of cell lines - (reply: 1)
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pBR322 relaxed plasmid....what type of media? - (reply: 3)
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does anyone know what "Postgate medium B" contains? - postgate medium B (reply: 1)
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Whats the best media to get the fastest E.coli growth at a low cost? (only conce - (reply: 3)
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Whats the best media to get the fastest E.coli growth at a low cost? (only conce - (reply: 4)
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overexpression and M9 media. - M9 minimal media and overexpression (reply: 1)
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What media would promote the best E.coli growth at a low cost for fermentation? - (reply: 18)
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adapting cells to CO2-Independent Medium - (reply: 1)
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how did it happen? - cells grow normally but when in SFM media, things get worse (reply: 9)
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3.5 kDa peptide expression in periplasm or medium - (reply: 1)
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Sauton's medium - (reply: 1)
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Mycoplasma Testing in culture medium - (reply: 2)
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OCT medium and real time pcr - (reply: 2)
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DMEM + FBS medium - (reply: 1)
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keratinocyte culture media - (reply: 8)
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how would you change suspension medium in wells - (reply: 1)
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SOC medium - acronym pedantry! - (reply: 20)
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Chemostat Media - (reply: 3)
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Add L-glutamine in cell culture medium? - (reply: 5)
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culture media left out for too long? - (reply: 7)
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Contamination from media (MRS) in PCR - (reply: 1)
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Protein analysis of conditioned media - How to prepare media before SDS-PAGE? (reply: 4)
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Problem preparing media containing MgSO4 and K2HPO4 - (reply: 3)
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medium composition - M9 media (reply: 1)
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Macrophage - Survival without changing media (reply: 3)
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how do you maintain a culture of N. gonorrhea? what type of media? - (reply: 2)
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Concentrating conditioned medium from cells - (reply: 6)
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protein free medium - (reply: 1)
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dumb question about SOB media - (reply: 2)
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pH balance - HCO3/CO2 buffer in culture media (reply: 1)
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Culture medium for MCF-10A - (reply: 4)
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sterilizing protein solutions + agar - how to sterilize sensitive media (reply: 4)
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Help with concentrating proteins in culture medium - (reply: 4)
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2X LB media - (reply: 2)
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Minimal Media Recipes - for Fed-Batch Cultures of EColi (reply: 1)
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Recipe for MM1 media - (reply: 2)
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G418 in freezing medium? - (reply: 2)
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M63 Minimal media - (reply: 1)
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Subculturing cells grown in serum free media - (reply: 2)
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Sauton's media recipe? - (reply: 7)
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Help: I left media at room temperature overnight - is it still good? (reply: 7)
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Function of beta-mercaptoethanol in cell culture medium - (reply: 6)
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Is this a bacterial Contamination? - White precipitate on surface of medium (reply: 1)
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what to add to media - cell culture medium (reply: 7)
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sodium pyruvate in medium - (reply: 3)
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Keratinocyte cultures with serum or serum free media - (reply: 1)
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What media used to dissolve polysaccharide extracts??? - What media should i used to dissolve the polysacch (reply: 1)
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best cell colture media for in vivo imaging - (reply: 3)
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role of LB agar and SOC media - (reply: 1)
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Concentration of secreted protein in culture media - Concentration of culture media (reply: 7)
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Protocol for mouse splenocytes - storage, media etc (reply: 2)
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ingredients of keratinocyte basal medium - (reply: 2)
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Animal origin free medium - Animal origin free medium (reply: 1)
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CO3 / HCO3 content of the culture medium - (reply: 3)
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Osmolarity of the mammalian cell medium - (reply: 2)
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Ingredient for keratinocyte culture medium - (reply: 1)
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Freezing medium and cell counting - (reply: 6)
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Tet resistant colonies will not grow in medium - (reply: 1)
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right medium and serum? - (reply: 2)
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Ca2+ or no Ca2+ in FACS medium? - discussion (reply: 1)
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Murine lymphoma media recommendations? - (reply: 1)
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O2 vs H2O2 - does H2O2 decease the O2 content in cell medium (reply: 1)
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growth media question - (reply: 3)
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Bugs grow on plates but not in media - (reply: 4)
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Minimal media - minimal media - isolation - biodegradative bact... (reply: 1)
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Dark LB media - (reply: 4)
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use Pen./Strep. in the media for trasnfection - (reply: 6)
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concentrate protein in culture media? - (reply: 2)
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Transfection - Changing Media - How often do I need to change media? (reply: 1)
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Protein Concentration from Medium - (reply: 2)
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media for Brassica napus - (reply: 1)
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Endotoxin in culture media - Endotoxin in culture media (reply: 2)
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Re: Low phosphate media forBacillus/Bacillus transformation protocol - (reply: 1)
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Low phosphate media forBacillus/Bacillus transformation protocol - (reply: 1)