media composition - (Feb/11/2008 )
i need help in making this media
Myelocult human stem cell long term culture:
•Fetal Bovine Serum
i get the components from the internet but i couldnt find the exact concentrations of each one of them ? any help?
Myelocult HCC-5100 appears to be a trade name for a culture medium from Stem Cells Technology. You probably will not find the exact composition listed. The primary literature on isolation and culture of the cells you are using will probably give you an idea of the composition you need. But you might be better served by purchasing the medium rather than trying to make it from scratch.
i know its better to buy it but i checked and iys really expensive so thats why my professor said to try and make it.
Do you know what company the cells came from? If you call them they should give you the necessary components and concentrations.
MYELOID LONG-TERM CULTURE MEDIUM
FOR PRIMITIVE HUMAN HEMATOPOIETIC CELLS
This medium contains horse serum (12.5%), fetal bovine serum (12.5%) and 2-mercaptoethanol
(10-4 M) in alpha MEM supplemented with L-glutamine (2 mM), I-inositol (0.16 mM) and folic acid
(16 μM). These components have been pretested and selected for their ability to optimally initiate and
maintain myelopoiesis in long-term cultures of human bone marrow.1-5
Half-media changes at weekly intervals should sustain myelopoiesis for at least 5 - 8 weeks.
Also, check out the PDF:
THANK U SOOOOOOOOOOOOOOO MUCH