Minimal media question - Minimal media for B.subtilis and B.cereus (Nov/05/2008 )
I am doing an honours project using spores of B.subtilis and B.cereus.
I need to prepare a minimal media which does not stimulate germination but supports growth of the vegetative cells. I'm having a really hard time finding out what type of minimal media is suitable for these organisms and my requirements. Someone please help cause i am ready for pulling my hair out!
The most simple media on which you can grow these organisms is a Nutrient agar media.
We isolate a good number of Bacillus subtilis as well as B.cereus from clinical specimens using the media along with BA & CA.