growing up plant seedlings in MS media - (Aug/20/2006 )
Hello, I hope someone can aid me clarification. I have plated seedlings from Arabidopsis thaliana in small petri-dish plates filled with MS media. I am still practicing my plating technique, along with doing actual experiments. In some plates, the problem was that I accidentally scraped the MS media when I was putting my seedling on there. My technique was sterile enough, but I dug into the media in some of them when plating a seedling.
In the end, there were quite a few plates that ended up with media being scraped or broken because I was trying to keep only one seedling on each square of the plate. I did not remove any media. It is only that the media in there has been dug in. Once you break the gel-like media, it is hard to reseal it. My question is has anyone had this experience, and does this affect plant growth on the plates?
hii
i had limited experiece in plant tissue culture
but i had tried keeping leaf discs and callus into MS media
and yes sometimes the media has been broken
but it has not really affected the growth
but of course some part of the explant was on the surface
i am not sure about seeds ,, mostly it shouldnt have any effect
my guess
if u have done it in sterile condtions
no problems at all
all the best
regards
laxmi
In the end, there were quite a few plates that ended up with media being scraped or broken because I was trying to keep only one seedling on each square of the plate. I did not remove any media. It is only that the media in there has been dug in. Once you break the gel-like media, it is hard to reseal it. My question is has anyone had this experience, and does this affect plant growth on the plates?
No this should have no effect on your seedling germination. The solid nature of the media only acts as a support for the germinating seedling, In essence synthetic soil so breaking it will have no effect.
Hello,
I found this paper it might be of interest to you. Noren et al (2004) It describes a novel method fro Adropsis cultivation. When your seedlings have germinated , will you put them in a hydroponic system?
I found this paper it might be of interest to you. Noren et al (2004) It describes a novel method fro Adropsis cultivation. When your seedlings have germinated , will you put them in a hydroponic system?
if you want anyone to be able to view this paper, you will have to give more details (eg-journal, title).
[quote name='henrih' date='Dec 18 2007, 06:46 PM' post='120641']
Hello,
Is this the paper Norén H, Svensson P, Andersson B (2004) A convenient and versatile hydroponic cultivation system for Arabidopsis thaliana. Physiol Plant 121: 343–348. But cannot access it
You may want to grow them on one plate first and then transfer them to other plates just after germination. The 2 cotyledon stage. I do this on a regular basis and havent had any problems.
You also may want to exclude sucrose from your ms media... it stops contamination fairly easily and doesnt affect plant growth too much... unless your growing them to an advanced stage.
yes, there is no problem with this, seedlings would continue growing normally if u follow aseptic technique 