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G418 in freezing medium? - (Aug/03/2005 )


I have a stably transfected cell line which I maintain with G418, do I need to add G418 to the freefing medium when I make vials for freezing?

Thanks smile.gif



G418 in freezing media is not required.

Upon revival of frozen cells, u'll have to add G418 after 3-4 hrs of revival process.

hope it helps.


Ok, ill do that.

Thanks a lot! smile.gif