isolate yeast - i want to isolate yeast using gye medium but other fungi dominate the (Jun/13/2008 )

hello frnds i am doing project on alcohol n poly alcohol production by using yeast so pls can u provide some information

1)what r good source of obtaining yeast after honey?we r trying various fruits, so what should we do to optimize isolation of yeast

2)what media other than gye should i used.media of different composition

3)i tried to isolate yeast from skin of grapes but other fungus dominated, so what shoud i do to avoid then n any other helpful information u can provide.

thank u

try adding some cycloheximide - and for a high Aw source likke honey, I might trry some Czapek Dox agar