medium for competent cell - SOB vs SOC (Dec/07/2006 )
i usually use SOB meduim (SO:Mg++=100:1) to make E. coli DH5a competent cell.
but yesterday i mistakenly culture E. coli DH5a in SOC (SO:Mg++:Glucose=100:1:1) instead of SOB. is it safe to make competent cell using SOC medium???
i think there shudnt be any noticeable difference, SOC also works fine with alpha DH5 without problem. not a matter to worry much i guess.
I use SOC for DH5a and it works well.
i got 10*6 competency
I use LB...
I have used both, but find that for DH5 (in my hands), SOC works better...
we make it in LB and we get high efficient cells