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problem using IPL 41 medium - (May/15/2008 )

Hello everyone,

I am using IPL 41 medium with 10% FBS for culturing Sf9 cells. The problem is a lot of crystal - like deposits in the plate within 48 h. Why is this happening? I don't add yeastolate - can this contribute to the deposition I have observed?

Thanks in advance for all your suggestions...

-smartsunny-

QUOTE (smartsunny @ May 15 2008, 09:25 PM)
Hello everyone,

I am using IPL 41 medium with 10% FBS for culturing Sf9 cells. The problem is a lot of crystal - like deposits in the plate within 48 h. Why is this happening? I don't add yeastolate - can this contribute to the deposition I have observed?

Thanks in advance for all your suggestions...

may be you just discovered that your protein is secretory and crystallizes in IPL 41 media! Solve the structure biggrin.gif

Apology, no idea mate.

-cellcounter-

QUOTE (cellcounter @ May 15 2008, 11:40 PM)
QUOTE (smartsunny @ May 15 2008, 09:25 PM)
Hello everyone,


Thanks in advance for all your suggestions...

may be you just discovered that your protein is secretory and crystallizes in IPL 41 media! Solve the structure biggrin.gif

Apology, no idea mate.


Cellcounter, I am just growing these cells not performing any expression study (as yet). Thanks mate!!

-smartsunny-

QUOTE
In Science, when you ASSUME you make an ASS out of U and ME

Nice siggy, it is true everywhere, not only in science!

-cellcounter-