Adherent HEK293 serum-free protein-free medium - (Jan/30/2007 )

Hi!

Does anyone know a company producing serum-free and protein-free (so i guess chemically defined...) medium for HEK293 cells? I can only find medium for suspension cultures....any idea? Alternatives?

Thanks Stardust

-stardust-

QUOTE (stardust @ Jan 30 2007, 10:55 AM)

hi,
I used to starve my 293T cells for 24h-48h, and they don't like it but definitively survive...
I hope it helps

-toni towers-

starving is not an option they need to be fit to express my protein...any ideas?

-stardust-

Have you tried growing your adherent cells in medium optimised for suspension cultures? It might work, 293 are pretty easy to grow.

-vairus-

I haven't tried it yet because most of the manufacturers say don't do it (they are optimised to avaid cell clumps so cells can't adhere to flask anymore...). But I have found a Medium for adherent cells. I'll get it next week (Cambrex Pro293a) and try if my cells like it!

Thnaks stardust

-stardust-