Medium preparation L-15 for MDA-MB-231 - (Jan/08/2007 )
Hi,
Have a quick question for ya'll. We've been trying to culture MDA-MB-231 breast cancer cells in L-15 medium but for whatever reason they seem to be growing exceedingly slow. We had them at one point and were subculturing at a ratio of 1:10 twice a week so this is definately not normal. We've since had to make up a new batch of L-15 medium and I was curious if we had somehow left a constituent out. We've been using mediatech L-15 medium (50-045-PB) with L-glutamine added, reconstituted in water, with 10% FBS. No other supplements were added.
We grow our cells in a 5% CO2 incubator. But with this medium we have the flasks capped tightly.
Thanks for any help ya'll can give.
Leibovitz's L-15 medium is formulated for growth of cells in CO2 free system. You are trying to culture these cells in CO2 incubator, just grow them in humidified incubator without CO2.
Hope this helps.
i grow my mb-231 in RPMI with 10%fbs, hepes, and pen strep glut. never seen a problem with them in that media.
V
Have a quick question for ya'll. We've been trying to culture MDA-MB-231 breast cancer cells in L-15 medium but for whatever reason they seem to be growing exceedingly slow. We had them at one point and were subculturing at a ratio of 1:10 twice a week so this is definately not normal. We've since had to make up a new batch of L-15 medium and I was curious if we had somehow left a constituent out. We've been using mediatech L-15 medium (50-045-PB) with L-glutamine added, reconstituted in water, with 10% FBS. No other supplements were added.
We grow our cells in a 5% CO2 incubator. But with this medium we have the flasks capped tightly.
Thanks for any help ya'll can give.
I've always grown them in RPMI 1640, 10% FBS, 2% P/S, 1.25% L-glut, and 1% sodium pyruvate in a 5% CO2 incubator without any problems.