Media for HEK293 - (Apr/25/2007 )

Hi Guys,

I was wondering what people grow there HEK293 cells in- High glucose DMEM or low glucose DMEM.

My 293s grow beautifully in HG-DMEM, but look incredibly bad in LG-DMEM.

Thanks!

-babynekko-

QUOTE (babynekko @ Apr 25 2007, 01:11 AM)

Hi Guys,

I was wondering what people grow there HEK293 cells in- High glucose DMEM or low glucose DMEM.

My 293s grow beautifully in HG-DMEM, but look incredibly bad in LG-DMEM.

Thanks!

I always grow my HEK293s in ordinary MEM and have never had a problem

-auldmok-

I grow my HEK 293 cells in High glucose DMEM with an addition of 1X NEAA. Works great for me.

-Ricola-