pBR322 relaxed plasmid....what type of media? - (Dec/15/2005 )
Hello I have BL21 E coli strain and pBR322 plasmid which is the relaxed one....doesnt replicat4e efficiently...what type of media would you recommend? thanx a lot
Check Molecular Cloning for the chloramphenicol plasmid copy number amplification protocol.
sorry but you mean in this site protocol section...cant find it...
J. Sambrook and D. W. Russell, 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor, ME.
Previous editions refered to as Maniatis.