Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1291. LINE1 OFR2 Primer Design - (reply: 1)
1292. rna isolation manually - (reply: 2)
1293. phosphorylation of primers using PNK - (reply: 1)
1294. RT-PCR contamination issue - (reply: 3)
1295. What's the longest overhanging primer seq. you have used? - (reply: 2)
1296. Primer Concentration to lower Ct - (reply: 1)
1297. Primer Concentration Help - (reply: 4)
1298. Can normal sequencing services sequence >1kb product? - sequencing (reply: 1)
1299. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
1300. Primer Degradation - What exactly happens? (reply: 1)
1301. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
1302. PCR/DNA Extraction Problem - (reply: 5)
1303. Primers in 2 exons - (reply: 2)
1304. NTC appear in real time pcr - (reply: 6)
1305. RNA solutions - Calculating concentrations (reply: 9)
1306. PCR of AT-rich DNA - (reply: 1)
1307. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
1308. TBE Buffer? - (reply: 4)
1309. Overlapping PCR - really need help ! - (reply: 5)
1310. gel documentation sysytem - (reply: 7)
1311. internal standard - (reply: 5)
1312. Checking genomic contamination - different ways (reply: 4)
1313. Probe or Primer info for bovine AQP-1 - (reply: 6)
1314. PCR with Platinum Taq - product yield issues - (reply: 3)
1315. real time PCR - (reply: 7)
1316. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1317. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1318. Sample handling for RNA extraction - (reply: 1)
1319. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1320. Normalising RNA concentrations - Help (reply: 4)