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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1291. Biotinylated! - brief explanaion (reply: 2)
1292. what do i say?! - (reply: 2)
1293. Smearing on gel in PCR products - (reply: 5)
1294. 260/230 ratio: how low is good for running PCRs? - (reply: 2)
1295. introducing alanine mutations - (reply: 1)
1296. Hematin - calculation (reply: 1)
1297. How to generate sticky ends? - (reply: 3)
1298. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1299. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1300. Relative RT-PCR, multiplate and calibration - (reply: 4)
1301. Trying to measure small levels of mRNA - (reply: 3)
1302. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1303. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
1304. Relative RT-PCR - (reply: 2)
1305. Relative real time RT PCR - (reply: 2)
1306. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1307. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1308. IQ5 and sybr/FAM - (reply: 3)
1309. a simple primer problem - (reply: 5)
1310. Failure to introduce mutations using Overlap PCR - (reply: 3)
1311. no or poor amplification - (reply: 1)
1312. Primers from 3'UTR - (reply: 2)
1313. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
1314. Designing primers with ESTs - (reply: 2)
1315. Ct variation/Ct limit/rē values/efficiency errors - (reply: 2)
1316. Probe manufacturers, who makes best probes? - Where do you get your probes and are you happy with them? (reply: 3)
1317. Real Time vs Traditional PCR results - (reply: 1)
1318. The problems to identify mouse genotype with PCR - (reply: 1)
1319. non specific amplification - DNA amplification by PCR (reply: 1)
1320. RNA Extraction - Extraction of Yeast RNA from mice blood (reply: 1)