Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1291. Left polymerase out overnight - Will it still work? (reply: 1)
1292. very low expression of a sample vs standard curve efficiency - (reply: 1)
1293. Primer Reconstitution--does temperature matter? - (reply: 5)
1294. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
1295. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
1296. Oligonucleotide degradation - (reply: 1)
1297. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
1298. never get bands by pfu related enzymes - (reply: 2)
1299. Primer design for qPCR - (reply: 1)
1300. Primer-BLAST... - (reply: 4)
1301. reporting results of qPCR - (reply: 1)
1302. a question about 16s rRNA as reference in RT-qPCR - (reply: 1)
1303. PCR genomic DNA of high GC content - (reply: 6)
1304. question about qRT-PCR preparation - (reply: 1)
1305. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
1306. qPCR efficiency calculation - (reply: 1)
1307. qPCR on chIP product - (reply: 3)
1308. Ghost contamination in RT-PCR - Mystery that i can't solve. Help. (reply: 1)
1309. PCR--11kb fragment - (reply: 4)
1310. primer design tips - (reply: 3)
1311. GroEL from Wolbachia - GroEL from Wolbachia (reply: 2)
1312. PCR with Biotin Incorporation - (reply: 2)
1313. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
1314. pcr product - hello all (reply: 1)
1315. how to save sybr green reagents? - (reply: 3)
1316. amplification of GroEL from Wolbachia! - amplification of GroEL from Wolbachia! (reply: 2)
1317. Forgot to stop reverse transcription reaction - (reply: 4)
1318. PCR with long and complex primers - (reply: 2)
1319. Phospholylation of primers - (reply: 1)
1320. Re-using PCR plates? - (reply: 2)