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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1291. Stability of genomic DNA in 1x PBS - (reply: 2)
1292. primer design by generunner DG - (reply: 1)
1293. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
1294. degenerate pcr - (reply: 1)
1295. DNA degradation - (reply: 1)
1296. DNase 1 treratment of RNA - (reply: 11)
1297. Secondary structure in real time - (reply: 1)
1298. primers with fluoresent dye PCR cycle question - (reply: 4)
1299. House keeping gene question? - Thanks for any help! (reply: 1)
1300. Questions about Tween 20 - (reply: 1)
1301. General qRT-PCR question - (reply: 3)
1302. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
1303. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
1304. Smears on PCR products - (reply: 4)
1305. Dumb Reverse Transcription PCR Question - (reply: 2)
1306. qPCR absolute/relative quantification method question - (reply: 4)
1307. PCR-amplicon too short - (reply: 1)
1308. Reverse transcription PCR - (reply: 1)
1309. PCR/RT-PCR beads - (reply: 2)
1310. High Amplification Efficiency in Std. Curve - (reply: 5)
1311. Run PCR amplifications in agarose gels - (reply: 3)
1312. Problems with validating primers and low expression genes - (reply: 3)
1313. Compare different qPCR runs - really need help (reply: 8)
1314. General real time questions. - (reply: 1)
1315. Analysis HELP!!!! - (reply: 1)
1316. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1317. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1318. Mutagenesis woes - (reply: 3)
1319. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1320. intron/exon spanning primers - (reply: 1)