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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1291. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1292. primer dimer - (reply: 2)
1293. Genotyping conditioning-where to start? - (reply: 1)
1294. Marker migrates slower depending where is loaded - (reply: 2)
1295. PCR on degraded templates - (reply: 2)
1296. PCR help needed - (reply: 3)
1297. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
1298. Tm difference - (reply: 2)
1299. Genotyping - PCR not working (reply: 6)
1300. RT qPCR Newbie, Weird Results - (reply: 1)
1301. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
1302. light bands, dark smear, dark dimers - (reply: 2)
1303. PCR, followed by sequencing... - why ?? (reply: 3)
1304. qPCR and varying cDNA concentrations - (reply: 1)
1305. PCR program - strange extension step (reply: 2)
1306. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
1307. Trouble with GAPDH housekeeping gene - (reply: 3)
1308. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
1309. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
1310. Using BLAST to check primers - (reply: 17)
1311. qPCR and quantity of RNA - (reply: 2)
1312. pcr doesnt show any band for DNa walking - (reply: 8)
1313. How & where to prepare qPCR - (reply: 9)
1314. about qPCR plates sealing films - Axygen UC-500 vs. UC-200 (reply: 4)
1315. storing large amounts of PCR mastermix beforehand - question (reply: 3)
1316. questions about semi-quantitive PCR - weird result form semi-quantitive PCR (reply: 2)
1317. Effects of more cDNA in PCR??? - (reply: 1)
1318. PCR product disappears after restriction digestion - (reply: 4)
1319. Real Time PCR - excluding Ct - (reply: 2)
1320. PCR product looks less intense compared to others - should I increase the number of cycles? (reply: 4)