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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1291. Primer Degradation - What exactly happens? (reply: 1)
1292. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
1293. PCR/DNA Extraction Problem - (reply: 5)
1294. Primers in 2 exons - (reply: 2)
1295. NTC appear in real time pcr - (reply: 6)
1296. RNA solutions - Calculating concentrations (reply: 9)
1297. PCR of AT-rich DNA - (reply: 1)
1298. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
1299. TBE Buffer? - (reply: 4)
1300. Overlapping PCR - really need help ! - (reply: 5)
1301. gel documentation sysytem - (reply: 7)
1302. internal standard - (reply: 5)
1303. Checking genomic contamination - different ways (reply: 4)
1304. Probe or Primer info for bovine AQP-1 - (reply: 6)
1305. PCR with Platinum Taq - product yield issues - (reply: 3)
1306. real time PCR - (reply: 7)
1307. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1308. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1309. Sample handling for RNA extraction - (reply: 1)
1310. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1311. Normalising RNA concentrations - Help (reply: 4)
1312. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
1313. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
1314. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
1315. Having abnormal dissociation curve when performing qPCR - (reply: 3)
1316. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
1317. Please help me if you could - (reply: 5)
1318. Tm caculation for different PCRs?? - new, confused, and frustrated beginner (reply: 3)
1319. absolute quantification - (reply: 4)
1320. The Case of a Missing Band: PCR Issue - (reply: 4)