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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1291. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
1292. PCR/DNA Extraction Problem - (reply: 5)
1293. Primers in 2 exons - (reply: 2)
1294. NTC appear in real time pcr - (reply: 6)
1295. RNA solutions - Calculating concentrations (reply: 9)
1296. PCR of AT-rich DNA - (reply: 1)
1297. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
1298. TBE Buffer? - (reply: 4)
1299. Overlapping PCR - really need help ! - (reply: 5)
1300. gel documentation sysytem - (reply: 7)
1301. internal standard - (reply: 5)
1302. Checking genomic contamination - different ways (reply: 4)
1303. Probe or Primer info for bovine AQP-1 - (reply: 6)
1304. PCR with Platinum Taq - product yield issues - (reply: 3)
1305. real time PCR - (reply: 7)
1306. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1307. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1308. Sample handling for RNA extraction - (reply: 1)
1309. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1310. Normalising RNA concentrations - Help (reply: 4)
1311. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
1312. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
1313. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
1314. Having abnormal dissociation curve when performing qPCR - (reply: 3)
1315. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
1316. Please help me if you could - (reply: 5)
1317. Tm caculation for different PCRs?? - new, confused, and frustrated beginner (reply: 3)
1318. absolute quantification - (reply: 4)
1319. The Case of a Missing Band: PCR Issue - (reply: 4)
1320. Log 10 fold serial dilutions - (reply: 2)