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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1291. Data acquisition for qPCR - Endpoint of annealing or endpoint of amplification? (reply: 3)
1292. Can't re-PCR the PCR product - (reply: 5)
1293. Contamination in Multilplex? - (reply: 4)
1294. some questions about the component tab of ABI prism 7000 software and the dissoc - (reply: 2)
1295. Optimal annealing temperature - (reply: 2)
1296. Little to no expression in one group, how to analyze this? - (reply: 1)
1297. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
1298. Taqman real-time PCR - Taqman and contamination (reply: 4)
1299. silly control question - No RT controls (reply: 2)
1300. Long Primers for PCR - (reply: 2)
1301. Data Analysis for Real-time PCR - (reply: 2)
1302. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
1303. argh more pcr headaches! - (reply: 6)
1304. Annealing temperature - (reply: 3)
1305. Replicate inconsistencies in qPCR - (reply: 6)
1306. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
1307. Chlamydia PCR cycling conditions - (reply: 1)
1308. On-unit screen or PC…which is better? - (reply: 12)
1309. Primers going "loopy" - (reply: 2)
1310. Biotinylated! - brief explanaion (reply: 2)
1311. what do i say?! - (reply: 2)
1312. Smearing on gel in PCR products - (reply: 5)
1313. 260/230 ratio: how low is good for running PCRs? - (reply: 2)
1314. introducing alanine mutations - (reply: 1)
1315. Hematin - calculation (reply: 1)
1316. How to generate sticky ends? - (reply: 3)
1317. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1318. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1319. Relative RT-PCR, multiplate and calibration - (reply: 4)
1320. Trying to measure small levels of mRNA - (reply: 3)