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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1291. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
1292. TBE Buffer? - (reply: 4)
1293. Overlapping PCR - really need help ! - (reply: 5)
1294. gel documentation sysytem - (reply: 7)
1295. internal standard - (reply: 5)
1296. Checking genomic contamination - different ways (reply: 4)
1297. Probe or Primer info for bovine AQP-1 - (reply: 6)
1298. PCR with Platinum Taq - product yield issues - (reply: 3)
1299. real time PCR - (reply: 7)
1300. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1301. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1302. Sample handling for RNA extraction - (reply: 1)
1303. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1304. Normalising RNA concentrations - Help (reply: 4)
1305. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
1306. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
1307. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
1308. Having abnormal dissociation curve when performing qPCR - (reply: 3)
1309. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
1310. Please help me if you could - (reply: 5)
1311. Tm caculation for different PCRs?? - new, confused, and frustrated beginner (reply: 3)
1312. absolute quantification - (reply: 4)
1313. The Case of a Missing Band: PCR Issue - (reply: 4)
1314. Log 10 fold serial dilutions - (reply: 2)
1315. paranormal qpcr amplification activity - melting curves (reply: 3)
1316. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
1317. PCR template concentration - (reply: 6)
1318. PCR efficiency calculated by Linreg - (reply: 3)
1319. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
1320. Stability of genomic DNA in 1x PBS - (reply: 2)