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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1291. RT-PCR contamination issue - (reply: 3)
1292. What's the longest overhanging primer seq. you have used? - (reply: 2)
1293. Primer Concentration to lower Ct - (reply: 1)
1294. Primer Concentration Help - (reply: 4)
1295. Can normal sequencing services sequence >1kb product? - sequencing (reply: 1)
1296. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
1297. Primer Degradation - What exactly happens? (reply: 1)
1298. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
1299. PCR/DNA Extraction Problem - (reply: 5)
1300. Primers in 2 exons - (reply: 2)
1301. NTC appear in real time pcr - (reply: 6)
1302. RNA solutions - Calculating concentrations (reply: 9)
1303. PCR of AT-rich DNA - (reply: 1)
1304. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
1305. TBE Buffer? - (reply: 4)
1306. Overlapping PCR - really need help ! - (reply: 5)
1307. gel documentation sysytem - (reply: 7)
1308. internal standard - (reply: 5)
1309. Checking genomic contamination - different ways (reply: 4)
1310. Probe or Primer info for bovine AQP-1 - (reply: 6)
1311. PCR with Platinum Taq - product yield issues - (reply: 3)
1312. real time PCR - (reply: 7)
1313. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1314. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1315. Sample handling for RNA extraction - (reply: 1)
1316. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1317. Normalising RNA concentrations - Help (reply: 4)
1318. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
1319. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
1320. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)