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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1291. primers with fluoresent dye PCR cycle question - (reply: 4)
1292. House keeping gene question? - Thanks for any help! (reply: 1)
1293. Questions about Tween 20 - (reply: 1)
1294. General qRT-PCR question - (reply: 3)
1295. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
1296. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
1297. Smears on PCR products - (reply: 4)
1298. Dumb Reverse Transcription PCR Question - (reply: 2)
1299. qPCR absolute/relative quantification method question - (reply: 4)
1300. PCR-amplicon too short - (reply: 1)
1301. Reverse transcription PCR - (reply: 1)
1302. PCR/RT-PCR beads - (reply: 2)
1303. High Amplification Efficiency in Std. Curve - (reply: 5)
1304. Run PCR amplifications in agarose gels - (reply: 3)
1305. Problems with validating primers and low expression genes - (reply: 3)
1306. Compare different qPCR runs - really need help (reply: 8)
1307. General real time questions. - (reply: 1)
1308. Analysis HELP!!!! - (reply: 1)
1309. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1310. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1311. Mutagenesis woes - (reply: 3)
1312. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1313. intron/exon spanning primers - (reply: 1)
1314. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
1315. PCR product for sequencing - sample storage?? (reply: 2)
1316. Absolute quantification using Real Time PCR - (reply: 2)
1317. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
1318. High Ct value - what to do next? - (reply: 4)
1319. Biorad Mini Opticon - can use any 0.2ml tubes or Biorad only? (reply: 1)
1320. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)