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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1291. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
1292. Chlamydia PCR cycling conditions - (reply: 1)
1293. On-unit screen or PC…which is better? - (reply: 12)
1294. Primers going "loopy" - (reply: 2)
1295. Biotinylated! - brief explanaion (reply: 2)
1296. what do i say?! - (reply: 2)
1297. Smearing on gel in PCR products - (reply: 5)
1298. 260/230 ratio: how low is good for running PCRs? - (reply: 2)
1299. introducing alanine mutations - (reply: 1)
1300. Hematin - calculation (reply: 1)
1301. How to generate sticky ends? - (reply: 3)
1302. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1303. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1304. Relative RT-PCR, multiplate and calibration - (reply: 4)
1305. Trying to measure small levels of mRNA - (reply: 3)
1306. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1307. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
1308. Relative RT-PCR - (reply: 2)
1309. Relative real time RT PCR - (reply: 2)
1310. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1311. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1312. IQ5 and sybr/FAM - (reply: 3)
1313. a simple primer problem - (reply: 5)
1314. Failure to introduce mutations using Overlap PCR - (reply: 3)
1315. no or poor amplification - (reply: 1)
1316. Primers from 3'UTR - (reply: 2)
1317. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
1318. Designing primers with ESTs - (reply: 2)
1319. Ct variation/Ct limit/r˛ values/efficiency errors - (reply: 2)
1320. Probe manufacturers, who makes best probes? - Where do you get your probes and are you happy with them? (reply: 3)