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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1831. Gain! - (reply: 4)
1832. selection of internal control gene - (reply: 2)
1833. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1834. primer design - primer design question (reply: 2)
1835. Protocol for 16S rDNA real time PCR - (reply: 2)
1836. qPCR - Standard curves (reply: 3)
1837. cDNA spectrophotometric quantification - (reply: 8)
1838. Standard curve standardization - variation between runs (reply: 4)
1839. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1840. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1841. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1842. low gc primers - help with pcr using low gc primers (reply: 6)
1843. Exon-Exon Junction ? - (reply: 3)
1844. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1845. Primers and annealing temperature - (reply: 2)
1846. Bacteria gene expression - (reply: 2)
1847. Copy number calculations in real time PCR - (reply: 2)
1848. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1849. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1850. RT-PCR Negative controls - (reply: 1)
1851. Problems with qRT-PCR - (reply: 3)
1852. PCR with 60 bp primers - results in no product (reply: 6)
1853. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1854. How do you manually determine Ct value? - (reply: 1)
1855. Running gel after RT-PCR - (reply: 5)
1856. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1857. Etbr staining methods - (reply: 6)
1858. PCR problems.. - problems with conventional PCR (reply: 3)
1859. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1860. gDNA or cDNA amplification in RT-PCR - (reply: 3)