Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1831. RT-PCR vs. conventional PCR - (reply: 1)
1832. qPCR primer design - (reply: 5)
1833. Re-PCR problem - (reply: 3)
1834. PCR and gel purification problem - did I screw this up (reply: 4)
1835. touchdown pcr - (reply: 6)
1836. non specific pcr products - (reply: 4)
1837. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
1838. template concentration qPCR - (reply: 1)
1839. Anyone with experiences in 2 step PCR - (reply: 1)
1840. qRT-PCR Problems - (reply: 1)
1841. PCR works on lab strain but not patient sample - HIV related (reply: 4)
1842. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
1843. Genomic DNA copy number qPCR - Help with control (reply: 8)
1844. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
1845. re-amplification of a PCR product - is it recommended? (reply: 7)
1846. PCR machine with accurate temperature control? - (reply: 1)
1847. qRT-PCR with DMSO - (reply: 2)
1848. Gene-specific RT-PCR - (reply: 2)
1849. Gain! - (reply: 4)
1850. selection of internal control gene - (reply: 2)
1851. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1852. primer design - primer design question (reply: 2)
1853. Protocol for 16S rDNA real time PCR - (reply: 2)
1854. qPCR - Standard curves (reply: 3)
1855. cDNA spectrophotometric quantification - (reply: 8)
1856. Standard curve standardization - variation between runs (reply: 4)
1857. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1858. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1859. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1860. low gc primers - help with pcr using low gc primers (reply: 6)