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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1831. primer dimers - (reply: 5)
1832. are my RT-primers ruined? accidentally stored at 4 C - (reply: 1)
1833. acceptable range for ΔCt ? - (reply: 1)
1834. RT-PCR vs. conventional PCR - (reply: 1)
1835. qPCR primer design - (reply: 5)
1836. Re-PCR problem - (reply: 3)
1837. PCR and gel purification problem - did I screw this up (reply: 4)
1838. touchdown pcr - (reply: 6)
1839. non specific pcr products - (reply: 4)
1840. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
1841. template concentration qPCR - (reply: 1)
1842. Anyone with experiences in 2 step PCR - (reply: 1)
1843. qRT-PCR Problems - (reply: 1)
1844. PCR works on lab strain but not patient sample - HIV related (reply: 4)
1845. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
1846. Genomic DNA copy number qPCR - Help with control (reply: 8)
1847. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
1848. re-amplification of a PCR product - is it recommended? (reply: 7)
1849. PCR machine with accurate temperature control? - (reply: 1)
1850. qRT-PCR with DMSO - (reply: 2)
1851. Gene-specific RT-PCR - (reply: 2)
1852. Gain! - (reply: 4)
1853. selection of internal control gene - (reply: 2)
1854. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1855. primer design - primer design question (reply: 2)
1856. Protocol for 16S rDNA real time PCR - (reply: 2)
1857. qPCR - Standard curves (reply: 3)
1858. cDNA spectrophotometric quantification - (reply: 8)
1859. Standard curve standardization - variation between runs (reply: 4)
1860. How to recognize primer-dimers? - from a melting curve??... (reply: 4)