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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1831. Anyone with experiences in 2 step PCR - (reply: 1)
1832. qRT-PCR Problems - (reply: 1)
1833. PCR works on lab strain but not patient sample - HIV related (reply: 4)
1834. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
1835. Genomic DNA copy number qPCR - Help with control (reply: 8)
1836. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
1837. re-amplification of a PCR product - is it recommended? (reply: 7)
1838. PCR machine with accurate temperature control? - (reply: 1)
1839. qRT-PCR with DMSO - (reply: 2)
1840. Gene-specific RT-PCR - (reply: 2)
1841. Gain! - (reply: 4)
1842. selection of internal control gene - (reply: 2)
1843. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1844. primer design - primer design question (reply: 2)
1845. Protocol for 16S rDNA real time PCR - (reply: 2)
1846. qPCR - Standard curves (reply: 3)
1847. cDNA spectrophotometric quantification - (reply: 8)
1848. Standard curve standardization - variation between runs (reply: 4)
1849. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1850. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1851. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1852. low gc primers - help with pcr using low gc primers (reply: 6)
1853. Exon-Exon Junction ? - (reply: 3)
1854. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1855. Primers and annealing temperature - (reply: 2)
1856. Bacteria gene expression - (reply: 2)
1857. Copy number calculations in real time PCR - (reply: 2)
1858. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1859. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1860. RT-PCR Negative controls - (reply: 1)