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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1831. Re-PCR problem - (reply: 3)
1832. PCR and gel purification problem - did I screw this up (reply: 4)
1833. touchdown pcr - (reply: 6)
1834. non specific pcr products - (reply: 4)
1835. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
1836. template concentration qPCR - (reply: 1)
1837. Anyone with experiences in 2 step PCR - (reply: 1)
1838. qRT-PCR Problems - (reply: 1)
1839. PCR works on lab strain but not patient sample - HIV related (reply: 4)
1840. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
1841. Genomic DNA copy number qPCR - Help with control (reply: 8)
1842. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
1843. re-amplification of a PCR product - is it recommended? (reply: 7)
1844. PCR machine with accurate temperature control? - (reply: 1)
1845. qRT-PCR with DMSO - (reply: 2)
1846. Gene-specific RT-PCR - (reply: 2)
1847. Gain! - (reply: 4)
1848. selection of internal control gene - (reply: 2)
1849. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1850. primer design - primer design question (reply: 2)
1851. Protocol for 16S rDNA real time PCR - (reply: 2)
1852. qPCR - Standard curves (reply: 3)
1853. cDNA spectrophotometric quantification - (reply: 8)
1854. Standard curve standardization - variation between runs (reply: 4)
1855. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1856. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1857. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1858. low gc primers - help with pcr using low gc primers (reply: 6)
1859. Exon-Exon Junction ? - (reply: 3)
1860. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)