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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1831. re-amplification of a PCR product - is it recommended? (reply: 7)
1832. PCR machine with accurate temperature control? - (reply: 1)
1833. qRT-PCR with DMSO - (reply: 2)
1834. Gene-specific RT-PCR - (reply: 2)
1835. Gain! - (reply: 4)
1836. selection of internal control gene - (reply: 2)
1837. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1838. primer design - primer design question (reply: 2)
1839. Protocol for 16S rDNA real time PCR - (reply: 2)
1840. qPCR - Standard curves (reply: 3)
1841. cDNA spectrophotometric quantification - (reply: 8)
1842. Standard curve standardization - variation between runs (reply: 4)
1843. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1844. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1845. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1846. low gc primers - help with pcr using low gc primers (reply: 6)
1847. Exon-Exon Junction ? - (reply: 3)
1848. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1849. Primers and annealing temperature - (reply: 2)
1850. Bacteria gene expression - (reply: 2)
1851. Copy number calculations in real time PCR - (reply: 2)
1852. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1853. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1854. RT-PCR Negative controls - (reply: 1)
1855. Problems with qRT-PCR - (reply: 3)
1856. PCR with 60 bp primers - results in no product (reply: 6)
1857. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1858. How do you manually determine Ct value? - (reply: 1)
1859. Running gel after RT-PCR - (reply: 5)
1860. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)