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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. qPCR from HEK cells transfected with Plasmid - (reply: 4)
362. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
363. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
364. Negative Control Looks Like the Rest - (reply: 11)
365. Why RNase H treatment after cDNA synthesis - (reply: 2)
366. RNA isolation from Sesame seed - (reply: 3)
367. qPCR with Genomic DNA - (reply: 1)
368. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
369. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
370. Homogenize pancreas for RNA extraction - (reply: 3)
371. Point mutagenesis..... - (reply: 6)
372. No bands in PCR after DNase treatment - (reply: 4)
373. Overlap PCR problem - (reply: 5)
374. PCR product purification - (reply: 4)
375. Overlapping sequence PCR primers - (reply: 1)
376. acceptable melt curve? - (reply: 1)
377. Calibrator sample - (reply: 4)
378. Ct value very high. What can I do to improve this ct? - (reply: 4)
379. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
380. Amount of RNA (ng) for good ct values. - (reply: 3)
381. Normalizing qPCR data - (reply: 1)
382. doing PCR using PCR product , help plz !! - (reply: 5)
383. High GC content RT-qPCR - please help - (reply: 3)
384. Electrophoresis problem , please help pals ! - (reply: 8)
385. Calculating Standard Errors and Normalising to Transgene - (reply: 1)
386. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
387. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
388. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
389. iCycler Calibration Question - (reply: 1)
390. Long PCR (>10kb) polymerase recommendations - (reply: 3)