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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. What do you use to check primer secondary structure? - (reply: 3)
362. CT of qPCR - (reply: 3)
363. Need help with my real time RT-PCR Plate Set up - (reply: 8)
364. PCR product as template for in vitro transcription - (reply: 1)
365. Sybr Green vs Taqman -- a practical approach - (reply: 2)
366. Problem with 3 step PCR - (reply: 3)
367. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
368. ARMS-PCR - (reply: 1)
369. Scaling up PCR to get more DNA - (reply: 5)
370. RNA concentration suddenly drops - (reply: 3)
371. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
372. PCR to get 10kbp product - (reply: 4)
373. site-directed mutagenesis primer Tm - (reply: 4)
374. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
375. RNA concentration to start RT reaction - (reply: 1)
376. Random vs oligo primer in preamplification RT - (reply: 1)
377. Smart Cycler II software and Absolute quantification help - (reply: 1)
378. cDNA amount in qPCR - (reply: 2)
379. mRNA integrity for qPCR - (reply: 6)
380. Strange control contamination in cDNA synthesis - (reply: 2)
381. how does polymerase stop at the required length - (reply: 1)
382. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
383. Real-time efficiency question - (reply: 1)
384. Selective removal of standards post qPCR Run - (reply: 1)
385. Primer concentration - stupid question - (reply: 3)
386. Primer Design, help im New - (reply: 4)
387. qPCR question (no results?) - (reply: 1)
388. Artificial RNA as normalizer (HK gene) - (reply: 1)
389. Sensitivity of RT-PCR and qPCR - (reply: 4)
390. Mixing two cDNA samples into one for realtime PCR - (reply: 3)