Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
362. PCR product as standard curve template - (reply: 6)
363. TaqMan CN assay - control sample duplicated - (reply: 1)
364. qPCR - no amplification curve but suitable melting curve - (reply: 3)
365. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
366. Basic question about qPCR and endogenous RNA control. - (reply: 4)
367. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
368. something very basic, why called "event-specific detection" - (reply: 1)
369. skip 65 degrees protocol of Reverse transcription - (reply: 4)
370. Mystery in my PCR - (reply: 5)
371. Whole genome amplification from cDNA? - (reply: 4)
372. What do you use to check primer secondary structure? - (reply: 3)
373. CT of qPCR - (reply: 3)
374. Need help with my real time RT-PCR Plate Set up - (reply: 8)
375. PCR product as template for in vitro transcription - (reply: 1)
376. Sybr Green vs Taqman -- a practical approach - (reply: 2)
377. Problem with 3 step PCR - (reply: 3)
378. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
379. ARMS-PCR - (reply: 1)
380. Scaling up PCR to get more DNA - (reply: 5)
381. RNA concentration suddenly drops - (reply: 3)
382. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
383. PCR to get 10kbp product - (reply: 4)
384. site-directed mutagenesis primer Tm - (reply: 4)
385. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
386. RNA concentration to start RT reaction - (reply: 1)
387. Random vs oligo primer in preamplification RT - (reply: 1)
388. Smart Cycler II software and Absolute quantification help - (reply: 1)
389. cDNA amount in qPCR - (reply: 2)
390. mRNA integrity for qPCR - (reply: 6)