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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. GAPDH Ct value - (reply: 4)
362. dissociation curves - (reply: 1)
363. RT-PCR primer design - (reply: 7)
364. How to amplify very short PCR template - (reply: 4)
365. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
366. Is this primer okay? - (reply: 4)
367. Dilution calculation - (reply: 3)
368. Common causes for low RNA A260/230 ratios - (reply: 7)
369. Limit of detection for salmon gDNA? - (reply: 7)
370. Intensifying signal from positive control - (reply: 5)
371. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
372. analysing qpcr data and plotting standard curve - (reply: 1)
373. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
374. PCR product as standard curve template - (reply: 6)
375. TaqMan CN assay - control sample duplicated - (reply: 1)
376. qPCR - no amplification curve but suitable melting curve - (reply: 3)
377. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
378. Basic question about qPCR and endogenous RNA control. - (reply: 4)
379. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
380. something very basic, why called "event-specific detection" - (reply: 1)
381. skip 65 degrees protocol of Reverse transcription - (reply: 4)
382. Mystery in my PCR - (reply: 5)
383. Whole genome amplification from cDNA? - (reply: 4)
384. What do you use to check primer secondary structure? - (reply: 3)
385. CT of qPCR - (reply: 3)
386. Need help with my real time RT-PCR Plate Set up - (reply: 8)
387. PCR product as template for in vitro transcription - (reply: 1)
388. Sybr Green vs Taqman -- a practical approach - (reply: 2)
389. Problem with 3 step PCR - (reply: 3)
390. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)