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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. something very basic, why called "event-specific detection" - (reply: 1)
362. skip 65 degrees protocol of Reverse transcription - (reply: 4)
363. Mystery in my PCR - (reply: 5)
364. Whole genome amplification from cDNA? - (reply: 4)
365. What do you use to check primer secondary structure? - (reply: 3)
366. CT of qPCR - (reply: 3)
367. Need help with my real time RT-PCR Plate Set up - (reply: 8)
368. PCR product as template for in vitro transcription - (reply: 1)
369. Sybr Green vs Taqman -- a practical approach - (reply: 2)
370. Problem with 3 step PCR - (reply: 3)
371. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
372. ARMS-PCR - (reply: 1)
373. Scaling up PCR to get more DNA - (reply: 5)
374. RNA concentration suddenly drops - (reply: 3)
375. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
376. PCR to get 10kbp product - (reply: 4)
377. site-directed mutagenesis primer Tm - (reply: 4)
378. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
379. RNA concentration to start RT reaction - (reply: 1)
380. Random vs oligo primer in preamplification RT - (reply: 1)
381. Smart Cycler II software and Absolute quantification help - (reply: 1)
382. cDNA amount in qPCR - (reply: 2)
383. mRNA integrity for qPCR - (reply: 6)
384. Strange control contamination in cDNA synthesis - (reply: 2)
385. how does polymerase stop at the required length - (reply: 1)
386. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
387. Real-time efficiency question - (reply: 1)
388. Selective removal of standards post qPCR Run - (reply: 1)
389. Primer concentration - stupid question - (reply: 3)
390. Primer Design, help im New - (reply: 4)