Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
362. ARMS-PCR - (reply: 1)
363. Scaling up PCR to get more DNA - (reply: 5)
364. RNA concentration suddenly drops - (reply: 3)
365. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
366. PCR to get 10kbp product - (reply: 4)
367. site-directed mutagenesis primer Tm - (reply: 4)
368. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
369. RNA concentration to start RT reaction - (reply: 1)
370. Random vs oligo primer in preamplification RT - (reply: 1)
371. Smart Cycler II software and Absolute quantification help - (reply: 1)
372. cDNA amount in qPCR - (reply: 2)
373. mRNA integrity for qPCR - (reply: 6)
374. Strange control contamination in cDNA synthesis - (reply: 2)
375. how does polymerase stop at the required length - (reply: 1)
376. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
377. Real-time efficiency question - (reply: 1)
378. Selective removal of standards post qPCR Run - (reply: 1)
379. Primer concentration - stupid question - (reply: 3)
380. Primer Design, help im New - (reply: 4)
381. qPCR question (no results?) - (reply: 1)
382. Artificial RNA as normalizer (HK gene) - (reply: 1)
383. Sensitivity of RT-PCR and qPCR - (reply: 4)
384. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
385. real time PCR trouble - (reply: 3)
386. removal of ethanol in PCR product purification - (reply: 5)
387. Crazy real time PCR curve - (reply: 4)
388. High fidelity PCR trouble shooting - (reply: 2)
389. Reproducible Non-Specific PCR Product - (reply: 2)
390. How to prepare serial dilutions of RNA or cDNA - (reply: 3)