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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. Is this primer okay? - (reply: 4)
362. Dilution calculation - (reply: 3)
363. Common causes for low RNA A260/230 ratios - (reply: 7)
364. Limit of detection for salmon gDNA? - (reply: 7)
365. Intensifying signal from positive control - (reply: 5)
366. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
367. analysing qpcr data and plotting standard curve - (reply: 1)
368. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
369. PCR product as standard curve template - (reply: 6)
370. TaqMan CN assay - control sample duplicated - (reply: 1)
371. qPCR - no amplification curve but suitable melting curve - (reply: 3)
372. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
373. Basic question about qPCR and endogenous RNA control. - (reply: 4)
374. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
375. something very basic, why called "event-specific detection" - (reply: 1)
376. skip 65 degrees protocol of Reverse transcription - (reply: 4)
377. Mystery in my PCR - (reply: 5)
378. Whole genome amplification from cDNA? - (reply: 4)
379. What do you use to check primer secondary structure? - (reply: 3)
380. CT of qPCR - (reply: 3)
381. Need help with my real time RT-PCR Plate Set up - (reply: 8)
382. PCR product as template for in vitro transcription - (reply: 1)
383. Sybr Green vs Taqman -- a practical approach - (reply: 2)
384. Problem with 3 step PCR - (reply: 3)
385. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
386. ARMS-PCR - (reply: 1)
387. Scaling up PCR to get more DNA - (reply: 5)
388. RNA concentration suddenly drops - (reply: 3)
389. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
390. PCR to get 10kbp product - (reply: 4)