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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. Designing primers for ABO blood groups - (reply: 1)
362. Methylight Results Analysis - (reply: 1)
363. GAPDH Ct value - (reply: 4)
364. dissociation curves - (reply: 1)
365. RT-PCR primer design - (reply: 7)
366. How to amplify very short PCR template - (reply: 4)
367. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
368. Is this primer okay? - (reply: 4)
369. Dilution calculation - (reply: 3)
370. Common causes for low RNA A260/230 ratios - (reply: 7)
371. Limit of detection for salmon gDNA? - (reply: 7)
372. Intensifying signal from positive control - (reply: 5)
373. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
374. analysing qpcr data and plotting standard curve - (reply: 1)
375. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
376. PCR product as standard curve template - (reply: 6)
377. TaqMan CN assay - control sample duplicated - (reply: 1)
378. qPCR - no amplification curve but suitable melting curve - (reply: 3)
379. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
380. Basic question about qPCR and endogenous RNA control. - (reply: 4)
381. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
382. something very basic, why called "event-specific detection" - (reply: 1)
383. skip 65 degrees protocol of Reverse transcription - (reply: 4)
384. Mystery in my PCR - (reply: 5)
385. Whole genome amplification from cDNA? - (reply: 4)
386. What do you use to check primer secondary structure? - (reply: 3)
387. CT of qPCR - (reply: 3)
388. Need help with my real time RT-PCR Plate Set up - (reply: 8)
389. PCR product as template for in vitro transcription - (reply: 1)
390. Sybr Green vs Taqman -- a practical approach - (reply: 2)