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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. Adding tag using overlapping PCR - (reply: 2)
362. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
363. Design primer from incomplete sequence... - (reply: 2)
364. Long term storage for RNA extracted from pancreas - (reply: 3)
365. How to perform colony PCR - (reply: 1)
366. qPCR from HEK cells transfected with Plasmid - (reply: 4)
367. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
368. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
369. Negative Control Looks Like the Rest - (reply: 11)
370. Why RNase H treatment after cDNA synthesis - (reply: 2)
371. RNA isolation from Sesame seed - (reply: 3)
372. qPCR with Genomic DNA - (reply: 1)
373. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
374. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
375. Homogenize pancreas for RNA extraction - (reply: 3)
376. Point mutagenesis..... - (reply: 6)
377. No bands in PCR after DNase treatment - (reply: 4)
378. Overlap PCR problem - (reply: 5)
379. PCR product purification - (reply: 4)
380. Overlapping sequence PCR primers - (reply: 1)
381. acceptable melt curve? - (reply: 1)
382. Calibrator sample - (reply: 4)
383. Ct value very high. What can I do to improve this ct? - (reply: 4)
384. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
385. Amount of RNA (ng) for good ct values. - (reply: 3)
386. Normalizing qPCR data - (reply: 1)
387. doing PCR using PCR product , help plz !! - (reply: 5)
388. High GC content RT-qPCR - please help - (reply: 3)
389. Electrophoresis problem , please help pals ! - (reply: 8)
390. Calculating Standard Errors and Normalising to Transgene - (reply: 1)