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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
362. 100 ng RNA for cDNA synthesis - (reply: 4)
363. Inexplicable qPCR failure in single wells - (reply: 12)
364. negative control well 300 bp band - (reply: 3)
365. Reference gene for normalisation - for different growth rates - (reply: 3)
366. Strange amplification plots with high Ct variability - (reply: 1)
367. How big a role does mixing play in PCR - (reply: 1)
368. Melting curve is irregular for primer optimization - (reply: 5)
369. Designing primers for ABO blood groups - (reply: 1)
370. Methylight Results Analysis - (reply: 1)
371. GAPDH Ct value - (reply: 4)
372. dissociation curves - (reply: 1)
373. RT-PCR primer design - (reply: 7)
374. How to amplify very short PCR template - (reply: 4)
375. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
376. Is this primer okay? - (reply: 4)
377. Dilution calculation - (reply: 3)
378. Common causes for low RNA A260/230 ratios - (reply: 7)
379. Limit of detection for salmon gDNA? - (reply: 7)
380. Intensifying signal from positive control - (reply: 5)
381. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
382. analysing qpcr data and plotting standard curve - (reply: 1)
383. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
384. PCR product as standard curve template - (reply: 6)
385. TaqMan CN assay - control sample duplicated - (reply: 1)
386. qPCR - no amplification curve but suitable melting curve - (reply: 3)
387. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
388. Basic question about qPCR and endogenous RNA control. - (reply: 4)
389. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
390. something very basic, why called "event-specific detection" - (reply: 1)