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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. PCR product as template for in vitro transcription - (reply: 1)
362. Sybr Green vs Taqman -- a practical approach - (reply: 2)
363. Problem with 3 step PCR - (reply: 3)
364. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
365. ARMS-PCR - (reply: 1)
366. Scaling up PCR to get more DNA - (reply: 5)
367. RNA concentration suddenly drops - (reply: 3)
368. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
369. PCR to get 10kbp product - (reply: 4)
370. site-directed mutagenesis primer Tm - (reply: 4)
371. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
372. RNA concentration to start RT reaction - (reply: 1)
373. Random vs oligo primer in preamplification RT - (reply: 1)
374. Smart Cycler II software and Absolute quantification help - (reply: 1)
375. cDNA amount in qPCR - (reply: 2)
376. mRNA integrity for qPCR - (reply: 6)
377. Strange control contamination in cDNA synthesis - (reply: 2)
378. how does polymerase stop at the required length - (reply: 1)
379. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
380. Real-time efficiency question - (reply: 1)
381. Selective removal of standards post qPCR Run - (reply: 1)
382. Primer concentration - stupid question - (reply: 3)
383. Primer Design, help im New - (reply: 4)
384. qPCR question (no results?) - (reply: 1)
385. Artificial RNA as normalizer (HK gene) - (reply: 1)
386. Sensitivity of RT-PCR and qPCR - (reply: 4)
387. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
388. real time PCR trouble - (reply: 3)
389. removal of ethanol in PCR product purification - (reply: 5)
390. Crazy real time PCR curve - (reply: 4)