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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. Strange amplification plots with high Ct variability - (reply: 1)
362. How big a role does mixing play in PCR - (reply: 1)
363. Melting curve is irregular for primer optimization - (reply: 5)
364. Designing primers for ABO blood groups - (reply: 1)
365. Methylight Results Analysis - (reply: 1)
366. GAPDH Ct value - (reply: 4)
367. dissociation curves - (reply: 1)
368. RT-PCR primer design - (reply: 7)
369. How to amplify very short PCR template - (reply: 4)
370. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
371. Is this primer okay? - (reply: 4)
372. Dilution calculation - (reply: 3)
373. Common causes for low RNA A260/230 ratios - (reply: 7)
374. Limit of detection for salmon gDNA? - (reply: 7)
375. Intensifying signal from positive control - (reply: 5)
376. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
377. analysing qpcr data and plotting standard curve - (reply: 1)
378. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
379. PCR product as standard curve template - (reply: 6)
380. TaqMan CN assay - control sample duplicated - (reply: 1)
381. qPCR - no amplification curve but suitable melting curve - (reply: 3)
382. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
383. Basic question about qPCR and endogenous RNA control. - (reply: 4)
384. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
385. something very basic, why called "event-specific detection" - (reply: 1)
386. skip 65 degrees protocol of Reverse transcription - (reply: 4)
387. Mystery in my PCR - (reply: 5)
388. Whole genome amplification from cDNA? - (reply: 4)
389. What do you use to check primer secondary structure? - (reply: 3)
390. CT of qPCR - (reply: 3)