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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. Homogenize pancreas for RNA extraction - (reply: 3)
362. Point mutagenesis..... - (reply: 6)
363. No bands in PCR after DNase treatment - (reply: 4)
364. Overlap PCR problem - (reply: 5)
365. PCR product purification - (reply: 4)
366. Overlapping sequence PCR primers - (reply: 1)
367. acceptable melt curve? - (reply: 1)
368. Calibrator sample - (reply: 4)
369. Ct value very high. What can I do to improve this ct? - (reply: 4)
370. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
371. Amount of RNA (ng) for good ct values. - (reply: 3)
372. Normalizing qPCR data - (reply: 1)
373. doing PCR using PCR product , help plz !! - (reply: 5)
374. High GC content RT-qPCR - please help - (reply: 3)
375. Electrophoresis problem , please help pals ! - (reply: 8)
376. Calculating Standard Errors and Normalising to Transgene - (reply: 1)
377. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
378. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
379. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
380. iCycler Calibration Question - (reply: 1)
381. Long PCR (>10kb) polymerase recommendations - (reply: 3)
382. thermocycler - real-time versus conventional? - (reply: 2)
383. I need help for designing a pair of primers for this sequence. - (reply: 1)
384. Change in Superscript lot influences transcript level of one gene but not others - (reply: 2)
385. 1/delta Ct vs delta delat CT - (reply: 1)
386. PCR band slightly BELOW expected length ?! - (reply: 14)
387. Does anyone have any problem with Quanta Sybr Green - (reply: 2)
388. Trouble correlating GU/ml qPCR results to cfu/ml plating results - (reply: 2)
389. long primers PCR - (reply: 4)
390. unexpected band in PCR with plasmid - (reply: 4)