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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. Scaling up PCR to get more DNA - (reply: 5)
362. RNA concentration suddenly drops - (reply: 3)
363. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
364. PCR to get 10kbp product - (reply: 4)
365. site-directed mutagenesis primer Tm - (reply: 4)
366. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
367. RNA concentration to start RT reaction - (reply: 1)
368. Random vs oligo primer in preamplification RT - (reply: 1)
369. Smart Cycler II software and Absolute quantification help - (reply: 1)
370. cDNA amount in qPCR - (reply: 2)
371. mRNA integrity for qPCR - (reply: 6)
372. Strange control contamination in cDNA synthesis - (reply: 2)
373. how does polymerase stop at the required length - (reply: 1)
374. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
375. Real-time efficiency question - (reply: 1)
376. Selective removal of standards post qPCR Run - (reply: 1)
377. Primer concentration - stupid question - (reply: 3)
378. Primer Design, help im New - (reply: 4)
379. qPCR question (no results?) - (reply: 1)
380. Artificial RNA as normalizer (HK gene) - (reply: 1)
381. Sensitivity of RT-PCR and qPCR - (reply: 4)
382. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
383. real time PCR trouble - (reply: 3)
384. removal of ethanol in PCR product purification - (reply: 5)
385. Crazy real time PCR curve - (reply: 4)
386. High fidelity PCR trouble shooting - (reply: 2)
387. Reproducible Non-Specific PCR Product - (reply: 2)
388. How to prepare serial dilutions of RNA or cDNA - (reply: 3)
389. Are these primer products good enough for qPCR? - (reply: 3)
390. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)