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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
362. PCR to get 10kbp product - (reply: 4)
363. site-directed mutagenesis primer Tm - (reply: 4)
364. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
365. RNA concentration to start RT reaction - (reply: 1)
366. Random vs oligo primer in preamplification RT - (reply: 1)
367. Smart Cycler II software and Absolute quantification help - (reply: 1)
368. cDNA amount in qPCR - (reply: 2)
369. mRNA integrity for qPCR - (reply: 6)
370. Strange control contamination in cDNA synthesis - (reply: 2)
371. how does polymerase stop at the required length - (reply: 1)
372. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
373. Real-time efficiency question - (reply: 1)
374. Selective removal of standards post qPCR Run - (reply: 1)
375. Primer concentration - stupid question - (reply: 3)
376. Primer Design, help im New - (reply: 4)
377. qPCR question (no results?) - (reply: 1)
378. Artificial RNA as normalizer (HK gene) - (reply: 1)
379. Sensitivity of RT-PCR and qPCR - (reply: 4)
380. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
381. real time PCR trouble - (reply: 3)
382. removal of ethanol in PCR product purification - (reply: 5)
383. Crazy real time PCR curve - (reply: 4)
384. High fidelity PCR trouble shooting - (reply: 2)
385. Reproducible Non-Specific PCR Product - (reply: 2)
386. How to prepare serial dilutions of RNA or cDNA - (reply: 3)
387. Are these primer products good enough for qPCR? - (reply: 3)
388. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
389. Can't get PCR with large overhang primers to work - (reply: 8)
390. Primer Efficiency across runs - (reply: 1)