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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
362. Basic question about qPCR and endogenous RNA control. - (reply: 4)
363. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
364. something very basic, why called "event-specific detection" - (reply: 1)
365. skip 65 degrees protocol of Reverse transcription - (reply: 4)
366. Mystery in my PCR - (reply: 5)
367. Whole genome amplification from cDNA? - (reply: 4)
368. What do you use to check primer secondary structure? - (reply: 3)
369. CT of qPCR - (reply: 3)
370. Need help with my real time RT-PCR Plate Set up - (reply: 8)
371. PCR product as template for in vitro transcription - (reply: 1)
372. Sybr Green vs Taqman -- a practical approach - (reply: 2)
373. Problem with 3 step PCR - (reply: 3)
374. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
375. ARMS-PCR - (reply: 1)
376. Scaling up PCR to get more DNA - (reply: 5)
377. RNA concentration suddenly drops - (reply: 3)
378. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
379. PCR to get 10kbp product - (reply: 4)
380. site-directed mutagenesis primer Tm - (reply: 4)
381. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
382. RNA concentration to start RT reaction - (reply: 1)
383. Random vs oligo primer in preamplification RT - (reply: 1)
384. Smart Cycler II software and Absolute quantification help - (reply: 1)
385. cDNA amount in qPCR - (reply: 2)
386. mRNA integrity for qPCR - (reply: 6)
387. Strange control contamination in cDNA synthesis - (reply: 2)
388. how does polymerase stop at the required length - (reply: 1)
389. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
390. Real-time efficiency question - (reply: 1)