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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
361. Inexplicable qPCR failure in single wells - (reply: 12)
362. negative control well 300 bp band - (reply: 3)
363. Reference gene for normalisation - for different growth rates - (reply: 3)
364. Strange amplification plots with high Ct variability - (reply: 1)
365. How big a role does mixing play in PCR - (reply: 1)
366. Melting curve is irregular for primer optimization - (reply: 5)
367. Designing primers for ABO blood groups - (reply: 1)
368. Methylight Results Analysis - (reply: 1)
369. GAPDH Ct value - (reply: 4)
370. dissociation curves - (reply: 1)
371. RT-PCR primer design - (reply: 7)
372. How to amplify very short PCR template - (reply: 4)
373. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
374. Is this primer okay? - (reply: 4)
375. Dilution calculation - (reply: 3)
376. Common causes for low RNA A260/230 ratios - (reply: 7)
377. Limit of detection for salmon gDNA? - (reply: 7)
378. Intensifying signal from positive control - (reply: 5)
379. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
380. analysing qpcr data and plotting standard curve - (reply: 1)
381. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
382. PCR product as standard curve template - (reply: 6)
383. TaqMan CN assay - control sample duplicated - (reply: 1)
384. qPCR - no amplification curve but suitable melting curve - (reply: 3)
385. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
386. Basic question about qPCR and endogenous RNA control. - (reply: 4)
387. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
388. something very basic, why called "event-specific detection" - (reply: 1)
389. skip 65 degrees protocol of Reverse transcription - (reply: 4)
390. Mystery in my PCR - (reply: 5)