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qPCR efficiency calculation - (Jul/03/2010 )

Hello everyone.
I would like to know how to calculate the efficiency of the qPCR. The slope value is -3.46. Please help

-chn09-

hi,

the formula of the efficiency (in %) is E(%) = (10-1/slope -1) *100. so for your slope this should be 94.5 % which is pretty good :D

-tea-test-