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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
391. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
392. PCR amplification with new restriction sites troubleshooting - (reply: 2)
393. using cell line to generate standard curve - (reply: 6)
394. PCR DNA Concentration - (reply: 1)
395. 3' RACE creates too big band doesn't leave well - (reply: 1)
396. RT-PCR product- no band - (reply: 4)
397. Normalization factor - (reply: 3)
398. Understanding RACE PCR - (reply: 1)
399. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
400. Dye for qPCR - (reply: 3)
401. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
402. Wierd Bands after PCR....Confused - (reply: 9)
403. PCR with Plasmid recovered from filter paper - (reply: 6)
404. PCR amplified product size - (reply: 5)
405. Opinion: What fold change is actually considered meaningful - (reply: 2)
406. Buffers RNase decontamination - (reply: 1)
407. the storage time for primers - (reply: 9)
408. Annealing temperature for PCR - (reply: 8)
409. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
410. Nested PCR - (reply: 2)
411. random primers or oligodT - (reply: 4)
412. Confused about normalization - (reply: 2)
413. To design or use published primers? - (reply: 4)
414. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
415. PCR primer usage (Clonning & cDNA) - (reply: 2)
416. Taq polymerase - (reply: 7)
417. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
418. Which High-Fidelity polymerase is better? - (reply: 2)
419. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
420. reference gene for qPCR in E.coli - (reply: 2)