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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
391. RNA extraction - (reply: 3)
392. Normalization of Ct of interest to a reference Ct - (reply: 3)
393. urgent: need help with qPCR statistics - (reply: 2)
394. A question for Reverse transcription experts - (reply: 5)
395. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
396. PCR amplification with new restriction sites troubleshooting - (reply: 2)
397. using cell line to generate standard curve - (reply: 6)
398. PCR DNA Concentration - (reply: 1)
399. 3' RACE creates too big band doesn't leave well - (reply: 1)
400. RT-PCR product- no band - (reply: 4)
401. Normalization factor - (reply: 3)
402. Understanding RACE PCR - (reply: 1)
403. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
404. Dye for qPCR - (reply: 3)
405. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
406. Wierd Bands after PCR....Confused - (reply: 9)
407. PCR with Plasmid recovered from filter paper - (reply: 6)
408. PCR amplified product size - (reply: 5)
409. Opinion: What fold change is actually considered meaningful - (reply: 2)
410. Buffers RNase decontamination - (reply: 1)
411. the storage time for primers - (reply: 9)
412. Annealing temperature for PCR - (reply: 8)
413. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
414. Nested PCR - (reply: 2)
415. random primers or oligodT - (reply: 4)
416. Confused about normalization - (reply: 2)
417. To design or use published primers? - (reply: 4)
418. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
419. PCR primer usage (Clonning & cDNA) - (reply: 2)
420. Taq polymerase - (reply: 7)