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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
391. site-directed mutagenesis primer Tm - (reply: 4)
392. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
393. RNA concentration to start RT reaction - (reply: 1)
394. Random vs oligo primer in preamplification RT - (reply: 1)
395. Smart Cycler II software and Absolute quantification help - (reply: 1)
396. cDNA amount in qPCR - (reply: 2)
397. mRNA integrity for qPCR - (reply: 6)
398. Strange control contamination in cDNA synthesis - (reply: 2)
399. how does polymerase stop at the required length - (reply: 1)
400. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
401. Real-time efficiency question - (reply: 1)
402. Selective removal of standards post qPCR Run - (reply: 1)
403. Primer concentration - stupid question - (reply: 3)
404. Primer Design, help im New - (reply: 4)
405. qPCR question (no results?) - (reply: 1)
406. Artificial RNA as normalizer (HK gene) - (reply: 1)
407. Sensitivity of RT-PCR and qPCR - (reply: 4)
408. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
409. real time PCR trouble - (reply: 3)
410. removal of ethanol in PCR product purification - (reply: 5)
411. Crazy real time PCR curve - (reply: 4)
412. High fidelity PCR trouble shooting - (reply: 2)
413. Reproducible Non-Specific PCR Product - (reply: 2)
414. How to prepare serial dilutions of RNA or cDNA - (reply: 3)
415. Are these primer products good enough for qPCR? - (reply: 3)
416. mtDNA to nDNA Ratio gDNA - qPCR - (reply: 1)
417. Can't get PCR with large overhang primers to work - (reply: 8)
418. Primer Efficiency across runs - (reply: 1)
419. Question about pipet tips for PCR and rtPCR - (reply: 4)
420. Taqman Probe sequence problem - (reply: 4)