Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
391. Which one is better as reference gene base on these data - (reply: 3)
392. About the qPCRs and statistics - (reply: 2)
393. long fragment PCR - (reply: 2)
394. Replacing one reagent by another in a protocol (Please help) - (reply: 5)
395. RT-qPCR normalization - (reply: 3)
396. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
397. I need to break this cycle of PCR issues - (reply: 13)
398. Questions / Help with Fusion PCR - (reply: 8)
399. Controls for RT-PCR reactions - (reply: 2)
400. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
401. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
402. issues when designing PCR primers using PRIMER3 - (reply: 8)
403. Primer catalog/databasing - (reply: 1)
404. Nonrepetitive nested PCR - (reply: 2)
405. DNA or cDNA? - (reply: 1)
406. Difference in Ct value of replicates - (reply: 1)
407. TDNA Genotyping PCR program - (reply: 1)
408. Shift in peaks at dissociation curve - (reply: 4)
409. High resolution melting analysis-SNP genotyping. - (reply: 1)
410. PCR - (reply: 2)
411. nonspecific band at 50bp in PCR - (reply: 9)
412. cDNA sequencing - (reply: 2)
413. Maximum PCR product Tm - (reply: 6)
414. QuikChange with Different plasmids - (reply: 1)
415. Good melting curve but non-specific products appeared - (reply: 2)
416. Long Non-Specific PCR Products - (reply: 5)
417. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
418. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
419. fold change (how to represent it) - (reply: 1)
420. PCR - consistent false positive results - (reply: 6)