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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
391. Problem with 3 step PCR - (reply: 3)
392. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
393. ARMS-PCR - (reply: 1)
394. Scaling up PCR to get more DNA - (reply: 5)
395. RNA concentration suddenly drops - (reply: 3)
396. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
397. PCR to get 10kbp product - (reply: 4)
398. site-directed mutagenesis primer Tm - (reply: 4)
399. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
400. RNA concentration to start RT reaction - (reply: 1)
401. Random vs oligo primer in preamplification RT - (reply: 1)
402. Smart Cycler II software and Absolute quantification help - (reply: 1)
403. cDNA amount in qPCR - (reply: 2)
404. mRNA integrity for qPCR - (reply: 6)
405. Strange control contamination in cDNA synthesis - (reply: 2)
406. how does polymerase stop at the required length - (reply: 1)
407. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
408. Real-time efficiency question - (reply: 1)
409. Selective removal of standards post qPCR Run - (reply: 1)
410. Primer concentration - stupid question - (reply: 3)
411. Primer Design, help im New - (reply: 4)
412. qPCR question (no results?) - (reply: 1)
413. Artificial RNA as normalizer (HK gene) - (reply: 1)
414. Sensitivity of RT-PCR and qPCR - (reply: 4)
415. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
416. real time PCR trouble - (reply: 3)
417. removal of ethanol in PCR product purification - (reply: 5)
418. Crazy real time PCR curve - (reply: 4)
419. High fidelity PCR trouble shooting - (reply: 2)
420. Reproducible Non-Specific PCR Product - (reply: 2)