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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
391. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
392. Dye for qPCR - (reply: 3)
393. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
394. Wierd Bands after PCR....Confused - (reply: 9)
395. PCR with Plasmid recovered from filter paper - (reply: 6)
396. PCR amplified product size - (reply: 5)
397. Opinion: What fold change is actually considered meaningful - (reply: 2)
398. Buffers RNase decontamination - (reply: 1)
399. the storage time for primers - (reply: 9)
400. Annealing temperature for PCR - (reply: 8)
401. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
402. Nested PCR - (reply: 2)
403. random primers or oligodT - (reply: 4)
404. Confused about normalization - (reply: 2)
405. To design or use published primers? - (reply: 4)
406. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
407. PCR primer usage (Clonning & cDNA) - (reply: 2)
408. Taq polymerase - (reply: 7)
409. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
410. Which High-Fidelity polymerase is better? - (reply: 2)
411. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
412. reference gene for qPCR in E.coli - (reply: 2)
413. Problem amplifying viral gene - (reply: 5)
414. The same CT with different Quantity, why? - (reply: 8)
415. Adding tag using overlapping PCR - (reply: 2)
416. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
417. Design primer from incomplete sequence... - (reply: 2)
418. Long term storage for RNA extracted from pancreas - (reply: 3)
419. How to perform colony PCR - (reply: 1)
420. qPCR from HEK cells transfected with Plasmid - (reply: 4)