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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
391. Normalization of Ct of interest to a reference Ct - (reply: 3)
392. urgent: need help with qPCR statistics - (reply: 2)
393. A question for Reverse transcription experts - (reply: 5)
394. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
395. PCR amplification with new restriction sites troubleshooting - (reply: 2)
396. using cell line to generate standard curve - (reply: 6)
397. PCR DNA Concentration - (reply: 1)
398. 3' RACE creates too big band doesn't leave well - (reply: 1)
399. RT-PCR product- no band - (reply: 4)
400. Normalization factor - (reply: 3)
401. Understanding RACE PCR - (reply: 1)
402. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
403. Dye for qPCR - (reply: 3)
404. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
405. Wierd Bands after PCR....Confused - (reply: 9)
406. PCR with Plasmid recovered from filter paper - (reply: 6)
407. PCR amplified product size - (reply: 5)
408. Opinion: What fold change is actually considered meaningful - (reply: 2)
409. Buffers RNase decontamination - (reply: 1)
410. the storage time for primers - (reply: 9)
411. Annealing temperature for PCR - (reply: 8)
412. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
413. Nested PCR - (reply: 2)
414. random primers or oligodT - (reply: 4)
415. Confused about normalization - (reply: 2)
416. To design or use published primers? - (reply: 4)
417. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
418. PCR primer usage (Clonning & cDNA) - (reply: 2)
419. Taq polymerase - (reply: 7)
420. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)