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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
601. How to check primer sequences using BLAST and other tools? - (reply: 2)
602. real time PCR analysis in patient samples - (reply: 2)
603. Experiment plan for RT-qPCR internal reference search - (reply: 3)
604. Primer Check? - (reply: 2)
605. amplification of 14Kb - (reply: 1)
606. Validation of PCR primers/ probes - (reply: 6)
607. genomic DNA extraction from formalin fixed tissues (not embedded) for genotyping - (reply: 4)
608. Real time PCR sudenly not working - (reply: 1)
609. Buffer-composition One-Step RT-PCR - (reply: 4)
610. Two reverse primer sequences for a single forward primer - (reply: 1)
611. Tm variation - (reply: 1)
612. PCR with more than one primer - (reply: 1)
613. RAPD - PCR problem - (reply: 5)
614. Relative fold expression and Normalization fold expression - (reply: 2)
615. Strange Bands - (reply: 2)
616. Recommended tamplate DNA concentration for qPCR - (reply: 2)
617. RT / cDNA synthesis - (reply: 7)
618. How to best store PCR product? - (reply: 2)
619. smearing below band of interest - (reply: 5)
620. RT-PCR primer design - (reply: 1)
621. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
622. Odd gel run following PCR - (reply: 28)
623. Qiagen PCR Array Reagents? - (reply: 1)
624. Enough checks for gDNA contamination in qPCR? - (reply: 3)
625. plate stuck in ABI7300 - (reply: 8)
626. No or very weak band using 16s primers - (reply: 1)
627. No PCR amplification with b-actin primers - (reply: 1)
628. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
629. optical qPCR plates - (reply: 1)
630. Fold change significance - (reply: 2)