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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
601. Genotyping PCR primers - (reply: 1)
602. Standard curve using plasmid - (reply: 1)
603. How to compare these results? Please comment. - (reply: 5)
604. Primers too dilute - (reply: 1)
605. Amplification curves jagged - (reply: 1)
606. ddCT issue: no endogenous expression in control. - (reply: 4)
607. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
608. No PCR product in site-directed mutagenesis - (reply: 4)
609. Giardia/Crypto real time PCR late amplification - (reply: 1)
610. How to dilute the template DNA for PCR - (reply: 7)
611. Desired length of overlap region for OE-PCR - (reply: 6)
612. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
613. Need help for PCR - (reply: 5)
614. scorpion probe - (reply: 8)
615. Too low Ct value for reference gene !!! - (reply: 3)
616. unespecific band PCR from mouse genomic DNA - (reply: 9)
617. Help with primer design through PRIMER3 - (reply: 4)
618. Help with unknown band in restriction digest - (reply: 1)
619. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
620. 454 sequencing - (reply: 2)
621. Preference for larger of 2 bands? - (reply: 2)
622. Tm of primers - prediction and verification - (reply: 1)
623. acceptable variation for biological replicate - (reply: 2)
624. RT-PCR contamination in negative control. - (reply: 1)
625. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
626. Technical problem with ABI7300 - (reply: 4)
627. cdna synthesis - (reply: 1)
628. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
629. Help with primer design - (reply: 3)
630. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)