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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
601. amplification of 14Kb - (reply: 1)
602. Validation of PCR primers/ probes - (reply: 6)
603. genomic DNA extraction from formalin fixed tissues (not embedded) for genotyping - (reply: 4)
604. Real time PCR sudenly not working - (reply: 1)
605. Buffer-composition One-Step RT-PCR - (reply: 4)
606. Two reverse primer sequences for a single forward primer - (reply: 1)
607. Tm variation - (reply: 1)
608. PCR with more than one primer - (reply: 1)
609. RAPD - PCR problem - (reply: 5)
610. Relative fold expression and Normalization fold expression - (reply: 2)
611. Strange Bands - (reply: 2)
612. Recommended tamplate DNA concentration for qPCR - (reply: 2)
613. RT / cDNA synthesis - (reply: 7)
614. How to best store PCR product? - (reply: 2)
615. smearing below band of interest - (reply: 5)
616. RT-PCR primer design - (reply: 1)
617. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
618. Odd gel run following PCR - (reply: 28)
619. Qiagen PCR Array Reagents? - (reply: 1)
620. Enough checks for gDNA contamination in qPCR? - (reply: 3)
621. plate stuck in ABI7300 - (reply: 8)
622. No or very weak band using 16s primers - (reply: 1)
623. No PCR amplification with b-actin primers - (reply: 1)
624. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
625. optical qPCR plates - (reply: 1)
626. Fold change significance - (reply: 2)
627. No PCR amplified with long primers - (reply: 10)
628. How many real time-PCR reactions for statistics and how? - (reply: 4)
629. PCR optimization: PCR vs qPCR - (reply: 4)
630. PCR smear for genomic samples - (reply: 4)