Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
601. Normalization of Real time PCR results using Pfaffl method - (reply: 2)
602. RT-PCR vs plate reader for pathogen detection - (reply: 3)
603. How to check primer sequences using BLAST and other tools? - (reply: 2)
604. real time PCR analysis in patient samples - (reply: 2)
605. Experiment plan for RT-qPCR internal reference search - (reply: 3)
606. Primer Check? - (reply: 2)
607. amplification of 14Kb - (reply: 1)
608. Validation of PCR primers/ probes - (reply: 6)
609. genomic DNA extraction from formalin fixed tissues (not embedded) for genotyping - (reply: 4)
610. Real time PCR sudenly not working - (reply: 1)
611. Buffer-composition One-Step RT-PCR - (reply: 4)
612. Two reverse primer sequences for a single forward primer - (reply: 1)
613. Tm variation - (reply: 1)
614. PCR with more than one primer - (reply: 1)
615. RAPD - PCR problem - (reply: 5)
616. Relative fold expression and Normalization fold expression - (reply: 2)
617. Strange Bands - (reply: 2)
618. Recommended tamplate DNA concentration for qPCR - (reply: 2)
619. RT / cDNA synthesis - (reply: 7)
620. How to best store PCR product? - (reply: 2)
621. smearing below band of interest - (reply: 5)
622. RT-PCR primer design - (reply: 1)
623. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
624. Odd gel run following PCR - (reply: 28)
625. Qiagen PCR Array Reagents? - (reply: 1)
626. Enough checks for gDNA contamination in qPCR? - (reply: 3)
627. plate stuck in ABI7300 - (reply: 8)
628. No or very weak band using 16s primers - (reply: 1)
629. No PCR amplification with b-actin primers - (reply: 1)
630. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)