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601. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
602. cDNA synthesis - (reply: 6)
603. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
604. Primer seq - (reply: 2)
605. Selection of housekeeping gene in Mycobacterium - (reply: 1)
606. Do I need to pool the base line samples together - (reply: 2)
607. PCR product size - (reply: 6)
608. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
609. Few basic questions about RNA - (reply: 3)
610. oligo dt and random primer - (reply: 3)
611. Ct's of goi higher and lower than ct's hkg - (reply: 1)
612. Effect of collagenase on qpcr results - (reply: 1)
613. RNA Isolation - (reply: 3)
614. mRNA Search for RT-PCR (U to T) - (reply: 2)
615. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
616. PCR triplicates versus one reaction - differences? (reply: 17)
617. cloning of race products - (reply: 1)
618. Single or double-stranded cDNA? - (reply: 2)
619. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
620. qPCR inhibition - (reply: 1)
621. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
622. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
623. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
624. Primers and self and hetero dimers - (reply: 1)
625. Really weird amplification curves - (reply: 1)
626. Primers for qPCR - (reply: 3)
627. copy number calculation from cDNA - (reply: 1)
628. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
629. RT-PCR primer - (reply: 3)
630. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
602. cDNA synthesis - (reply: 6)
603. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
604. Primer seq - (reply: 2)
605. Selection of housekeeping gene in Mycobacterium - (reply: 1)
606. Do I need to pool the base line samples together - (reply: 2)
607. PCR product size - (reply: 6)
608. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
609. Few basic questions about RNA - (reply: 3)
610. oligo dt and random primer - (reply: 3)
611. Ct's of goi higher and lower than ct's hkg - (reply: 1)
612. Effect of collagenase on qpcr results - (reply: 1)
613. RNA Isolation - (reply: 3)
614. mRNA Search for RT-PCR (U to T) - (reply: 2)
615. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
616. PCR triplicates versus one reaction - differences? (reply: 17)
617. cloning of race products - (reply: 1)
618. Single or double-stranded cDNA? - (reply: 2)
619. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
620. qPCR inhibition - (reply: 1)
621. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
622. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
623. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
624. Primers and self and hetero dimers - (reply: 1)
625. Really weird amplification curves - (reply: 1)
626. Primers for qPCR - (reply: 3)
627. copy number calculation from cDNA - (reply: 1)
628. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
629. RT-PCR primer - (reply: 3)
630. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)