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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
601. cDNA concentration of qPCR - (reply: 1)
602. pGEX vector - (reply: 2)
603. will the template of PCR be added A ? - (reply: 2)
604. qPCR normalization of reference gene - (reply: 1)
605. Primer design: Tm above or below Ta? - (reply: 5)
606. Design primers for expression cloning - (reply: 11)
607. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
608. primer dilution query - (reply: 2)
609. Real Time PCR polymerase enzyme - (reply: 3)
610. housekeeping gene has not uniform pattern - (reply: 3)
611. Using BLAST to check primers - (reply: 1)
612. What is the function of a third primer in a PCR mix?? - (reply: 2)
613. How can we design a primer ? step by step method - (reply: 4)
614. Desperate! qPCR standard curve problems - (reply: 2)
615. cDNA synthesis or RT-PCR - (reply: 3)
616. PCR on cDNA and SGamplification??? - (reply: 1)
617. real time PCR protocol - controls? - (reply: 2)
618. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
619. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
620. SOS: new real time PCR machine needed.... which one? - (reply: 5)
621. Amplicon side products - (reply: 5)
622. Fold change graph - (reply: 1)
623. Statistical analyses of 2 - delta delta Ct values - (reply: 2)
624. Finding the right sequence - (reply: 4)
625. Buy BAC vectors - (reply: 1)
626. meaningless real time results - (reply: 1)
627. RT-PCR in a single tube - (reply: 3)
628. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
629. annealing temperature for 3 primer pairs? - (reply: 4)
630. Heated-lid - (reply: 3)