Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
601. Relative fold expression and Normalization fold expression - (reply: 2)
602. Strange Bands - (reply: 2)
603. Recommended tamplate DNA concentration for qPCR - (reply: 2)
604. RT / cDNA synthesis - (reply: 7)
605. How to best store PCR product? - (reply: 2)
606. smearing below band of interest - (reply: 5)
607. RT-PCR primer design - (reply: 1)
608. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
609. Odd gel run following PCR - (reply: 28)
610. Qiagen PCR Array Reagents? - (reply: 1)
611. Enough checks for gDNA contamination in qPCR? - (reply: 3)
612. plate stuck in ABI7300 - (reply: 7)
613. No or very weak band using 16s primers - (reply: 1)
614. No PCR amplification with b-actin primers - (reply: 1)
615. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
616. optical qPCR plates - (reply: 1)
617. Fold change significance - (reply: 2)
618. No PCR amplified with long primers - (reply: 10)
619. How many real time-PCR reactions for statistics and how? - (reply: 4)
620. PCR optimization: PCR vs qPCR - (reply: 4)
621. PCR smear for genomic samples - (reply: 4)
622. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
623. Saliva and GCF DNA purification - (reply: 1)
624. rtPCR working but the elongated product wont gel! - (reply: 1)
625. another primer dilution question - (reply: 2)
626. TaqMan qPCR low-template issues - (reply: 1)
627. qPCR standard curve slope (m) - (reply: 2)
628. 16s double bands obtained-why?? - (reply: 2)
629. Application of RNase - (reply: 3)
630. i need help with virus pcr - (reply: 2)