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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
601. Preference for larger of 2 bands? - (reply: 2)
602. Tm of primers - prediction and verification - (reply: 1)
603. acceptable variation for biological replicate - (reply: 2)
604. RT-PCR contamination in negative control. - (reply: 1)
605. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
606. Technical problem with ABI7300 - (reply: 4)
607. cdna synthesis - (reply: 1)
608. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
609. Help with primer design - (reply: 3)
610. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)
611. PCR smearing and...problems! - (reply: 7)
612. Message Amount in qPCR - (reply: 1)
613. Running Primers on Agarose Gel - (reply: 6)
614. Explanation needed for melting curve - (reply: 3)
615. cDNA concentration of qPCR - (reply: 1)
616. pGEX vector - (reply: 2)
617. will the template of PCR be added A ? - (reply: 2)
618. qPCR normalization of reference gene - (reply: 1)
619. Primer design: Tm above or below Ta? - (reply: 5)
620. Design primers for expression cloning - (reply: 11)
621. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
622. primer dilution query - (reply: 2)
623. Real Time PCR polymerase enzyme - (reply: 3)
624. housekeeping gene has not uniform pattern - (reply: 3)
625. Using BLAST to check primers - (reply: 1)
626. What is the function of a third primer in a PCR mix?? - (reply: 2)
627. How can we design a primer ? step by step method - (reply: 4)
628. Desperate! qPCR standard curve problems - (reply: 2)
629. cDNA synthesis or RT-PCR - (reply: 3)
630. PCR on cDNA and SGamplification??? - (reply: 1)