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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
601. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
602. Odd gel run following PCR - (reply: 28)
603. Qiagen PCR Array Reagents? - (reply: 1)
604. Enough checks for gDNA contamination in qPCR? - (reply: 3)
605. plate stuck in ABI7300 - (reply: 7)
606. No or very weak band using 16s primers - (reply: 1)
607. No PCR amplification with b-actin primers - (reply: 1)
608. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
609. optical qPCR plates - (reply: 1)
610. Fold change significance - (reply: 2)
611. No PCR amplified with long primers - (reply: 10)
612. How many real time-PCR reactions for statistics and how? - (reply: 4)
613. PCR optimization: PCR vs qPCR - (reply: 4)
614. PCR smear for genomic samples - (reply: 4)
615. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
616. Saliva and GCF DNA purification - (reply: 1)
617. rtPCR working but the elongated product wont gel! - (reply: 1)
618. another primer dilution question - (reply: 2)
619. TaqMan qPCR low-template issues - (reply: 1)
620. qPCR standard curve slope (m) - (reply: 2)
621. 16s double bands obtained-why?? - (reply: 2)
622. Application of RNase - (reply: 3)
623. i need help with virus pcr - (reply: 2)
624. Primer Efficiency - (reply: 2)
625. data analysis for RT_PCR - (reply: 4)
626. Experimental set up for qRT PCR - (reply: 13)
627. What temp the lid of the thermocycler must be set? - (reply: 1)
628. How should I optimize my PCR - (reply: 2)
629. Real time data analysis using 2 machines - (reply: 3)
630. Second round of PCR after gel extraction fails miserably - (reply: 3)