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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1231. help with ct of reference gene - (reply: 1)
1232. PCR mutagenesis of plasmid - (reply: 5)
1233. Primer designed for ARMS PCR - (reply: 2)
1234. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
1235. methylation specific PCR - Primers - (reply: 1)
1236. bacterial identification using real time PCR - (reply: 1)
1237. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
1238. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
1239. Problem with multiplex PCR - Troubleshooting (reply: 4)
1240. Questions about ChIP protocols - (reply: 1)
1241. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
1242. What’s wrong with my NTC - (reply: 2)
1243. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
1244. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
1245. PCR reamplification - (reply: 2)
1246. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
1247. question about pcr - (reply: 3)
1248. Standarization of HRM for methylation - (reply: 4)
1249. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
1250. How to construct a standard curve for real time PCR - (reply: 1)
1251. PCR with one primer - (reply: 1)
1252. cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
1253. First strand synthesis - (reply: 1)
1254. PCR contamination - (reply: 19)
1255. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1256. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1257. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1258. PCR not working - No amplification - (reply: 5)
1259. Quantification of PCR products - (reply: 1)
1260. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)