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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1231. trembling dissociation curves in HRM assays - (reply: 3)
1232. Fluorescein storage? - (reply: 2)
1233. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1234. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1235. TELO2 & GAPDH - (reply: 2)
1236. PCR -No band formation - (reply: 1)
1237. 3' race - (reply: 1)
1238. PCR AMPLIFICATION - (reply: 1)
1239. internal control for genomic DNA - (reply: 1)
1240. qPCR Data Analysis - Software (reply: 3)
1241. Gene expression confusion - (reply: 5)
1242. real time pcr need advise - (reply: 2)
1243. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1244. ChIP-qPCR shift in melt curve - (reply: 4)
1245. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
1246. use of housekeeping gene in RT PCR chIP - (reply: 3)
1247. what is the order you follow in the run ??? - in order to make the contamination risk the least (reply: 3)
1248. DNA pooling for PCR - Saving money PCR (reply: 7)
1249. Real Time PCR Standard curves - How many are required??? (reply: 1)
1250. pcr-rflp - (reply: 2)
1251. PCR need some help - (reply: 4)
1252. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
1253. Left polymerase out overnight - Will it still work? (reply: 1)
1254. very low expression of a sample vs standard curve efficiency - (reply: 1)
1255. Primer Reconstitution--does temperature matter? - (reply: 5)
1256. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
1257. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
1258. Oligonucleotide degradation - (reply: 1)
1259. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
1260. never get bands by pfu related enzymes - (reply: 2)