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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1231. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1232. Nested PCR question - (reply: 1)
1233. High Tm on primers - cannot get a product - (reply: 8)
1234. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1235. Help with Multiplex Nested PCR - (reply: 2)
1236. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1237. trembling dissociation curves in HRM assays - (reply: 3)
1238. Fluorescein storage? - (reply: 2)
1239. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1240. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1241. TELO2 & GAPDH - (reply: 2)
1242. PCR -No band formation - (reply: 1)
1243. 3' race - (reply: 1)
1244. PCR AMPLIFICATION - (reply: 1)
1245. internal control for genomic DNA - (reply: 1)
1246. qPCR Data Analysis - Software (reply: 3)
1247. Gene expression confusion - (reply: 5)
1248. real time pcr need advise - (reply: 2)
1249. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1250. ChIP-qPCR shift in melt curve - (reply: 4)
1251. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
1252. use of housekeeping gene in RT PCR chIP - (reply: 3)
1253. what is the order you follow in the run ??? - in order to make the contamination risk the least (reply: 3)
1254. DNA pooling for PCR - Saving money PCR (reply: 7)
1255. Real Time PCR Standard curves - How many are required??? (reply: 1)
1256. pcr-rflp - (reply: 2)
1257. PCR need some help - (reply: 4)
1258. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
1259. Left polymerase out overnight - Will it still work? (reply: 1)
1260. very low expression of a sample vs standard curve efficiency - (reply: 1)