Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1231. The Case of a Missing Band: PCR Issue - (reply: 4)
1232. Log 10 fold serial dilutions - (reply: 2)
1233. paranormal qpcr amplification activity - melting curves (reply: 3)
1234. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
1235. PCR template concentration - (reply: 6)
1236. PCR efficiency calculated by Linreg - (reply: 3)
1237. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
1238. Stability of genomic DNA in 1x PBS - (reply: 2)
1239. primer design by generunner DG - (reply: 1)
1240. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
1241. degenerate pcr - (reply: 1)
1242. DNA degradation - (reply: 1)
1243. DNase 1 treratment of RNA - (reply: 11)
1244. Secondary structure in real time - (reply: 1)
1245. primers with fluoresent dye PCR cycle question - (reply: 4)
1246. House keeping gene question? - Thanks for any help! (reply: 1)
1247. Questions about Tween 20 - (reply: 1)
1248. General qRT-PCR question - (reply: 3)
1249. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
1250. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
1251. Smears on PCR products - (reply: 4)
1252. Dumb Reverse Transcription PCR Question - (reply: 2)
1253. qPCR absolute/relative quantification method question - (reply: 4)
1254. PCR-amplicon too short - (reply: 1)
1255. Reverse transcription PCR - (reply: 1)
1256. PCR/RT-PCR beads - (reply: 2)
1257. High Amplification Efficiency in Std. Curve - (reply: 5)
1258. Run PCR amplifications in agarose gels - (reply: 3)
1259. Problems with validating primers and low expression genes - (reply: 3)
1260. Compare different qPCR runs - really need help (reply: 8)