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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1231. Dumb Reverse Transcription PCR Question - (reply: 2)
1232. qPCR absolute/relative quantification method question - (reply: 4)
1233. PCR-amplicon too short - (reply: 1)
1234. Reverse transcription PCR - (reply: 1)
1235. PCR/RT-PCR beads - (reply: 2)
1236. High Amplification Efficiency in Std. Curve - (reply: 5)
1237. Run PCR amplifications in agarose gels - (reply: 3)
1238. Problems with validating primers and low expression genes - (reply: 3)
1239. Compare different qPCR runs - really need help (reply: 8)
1240. General real time questions. - (reply: 1)
1241. Analysis HELP!!!! - (reply: 1)
1242. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1243. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1244. Mutagenesis woes - (reply: 3)
1245. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1246. intron/exon spanning primers - (reply: 1)
1247. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
1248. PCR product for sequencing - sample storage?? (reply: 2)
1249. Absolute quantification using Real Time PCR - (reply: 2)
1250. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
1251. High Ct value - what to do next? - (reply: 4)
1252. Biorad Mini Opticon - can use any 0.2ml tubes or Biorad only? (reply: 1)
1253. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
1254. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
1255. Designing Primers for multiple Isoforms - (reply: 1)
1256. GAPDH CT lower than target gene CT - (reply: 2)
1257. standards for absolute quantification qPCR - (reply: 1)
1258. RT-PCR standard curve dilutions - (reply: 2)
1259. more than one band - (reply: 1)
1260. Relative gene expression - Newbie at RT-qPCR! (reply: 3)