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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1231. bacterial identification using real time PCR - (reply: 1)
1232. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
1233. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
1234. Problem with multiplex PCR - Troubleshooting (reply: 4)
1235. Questions about ChIP protocols - (reply: 1)
1236. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
1237. What’s wrong with my NTC - (reply: 2)
1238. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
1239. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
1240. PCR reamplification - (reply: 2)
1241. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
1242. question about pcr - (reply: 3)
1243. Standarization of HRM for methylation - (reply: 4)
1244. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
1245. How to construct a standard curve for real time PCR - (reply: 1)
1246. PCR with one primer - (reply: 1)
1247. cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
1248. First strand synthesis - (reply: 1)
1249. PCR contamination - (reply: 19)
1250. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1251. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1252. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1253. PCR not working - No amplification - (reply: 5)
1254. Quantification of PCR products - (reply: 1)
1255. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)
1256. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1257. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1258. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1259. Nested PCR question - (reply: 1)
1260. High Tm on primers - cannot get a product - (reply: 8)