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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1231. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1232. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1233. TELO2 & GAPDH - (reply: 2)
1234. PCR -No band formation - (reply: 1)
1235. 3' race - (reply: 1)
1236. PCR AMPLIFICATION - (reply: 1)
1237. internal control for genomic DNA - (reply: 1)
1238. qPCR Data Analysis - Software (reply: 3)
1239. Gene expression confusion - (reply: 5)
1240. real time pcr need advise - (reply: 2)
1241. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1242. ChIP-qPCR shift in melt curve - (reply: 4)
1243. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
1244. use of housekeeping gene in RT PCR chIP - (reply: 3)
1245. what is the order you follow in the run ??? - in order to make the contamination risk the least (reply: 3)
1246. DNA pooling for PCR - Saving money PCR (reply: 7)
1247. Real Time PCR Standard curves - How many are required??? (reply: 1)
1248. pcr-rflp - (reply: 2)
1249. PCR need some help - (reply: 4)
1250. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
1251. Left polymerase out overnight - Will it still work? (reply: 1)
1252. very low expression of a sample vs standard curve efficiency - (reply: 1)
1253. Primer Reconstitution--does temperature matter? - (reply: 5)
1254. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
1255. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
1256. Oligonucleotide degradation - (reply: 1)
1257. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
1258. never get bands by pfu related enzymes - (reply: 2)
1259. Primer design for qPCR - (reply: 1)
1260. Primer-BLAST... - (reply: 4)