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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1231. PCR reamplification - (reply: 2)
1232. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
1233. question about pcr - (reply: 3)
1234. Standarization of HRM for methylation - (reply: 4)
1235. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
1236. How to construct a standard curve for real time PCR - (reply: 1)
1237. PCR with one primer - (reply: 1)
1238. cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
1239. First strand synthesis - (reply: 1)
1240. PCR contamination - (reply: 19)
1241. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1242. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1243. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1244. PCR not working - No amplification - (reply: 5)
1245. Quantification of PCR products - (reply: 1)
1246. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)
1247. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1248. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1249. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1250. Nested PCR question - (reply: 1)
1251. High Tm on primers - cannot get a product - (reply: 8)
1252. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1253. Help with Multiplex Nested PCR - (reply: 2)
1254. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1255. trembling dissociation curves in HRM assays - (reply: 3)
1256. Fluorescein storage? - (reply: 2)
1257. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1258. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1259. TELO2 & GAPDH - (reply: 2)
1260. PCR -No band formation - (reply: 1)