Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1231. Analysis HELP!!!! - (reply: 1)
1232. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1233. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1234. Mutagenesis woes - (reply: 3)
1235. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1236. intron/exon spanning primers - (reply: 1)
1237. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
1238. PCR product for sequencing - sample storage?? (reply: 2)
1239. Absolute quantification using Real Time PCR - (reply: 2)
1240. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
1241. High Ct value - what to do next? - (reply: 4)
1242. Biorad Mini Opticon - can use any 0.2ml tubes or Biorad only? (reply: 1)
1243. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
1244. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
1245. Designing Primers for multiple Isoforms - (reply: 1)
1246. GAPDH CT lower than target gene CT - (reply: 2)
1247. standards for absolute quantification qPCR - (reply: 1)
1248. RT-PCR standard curve dilutions - (reply: 2)
1249. more than one band - (reply: 1)
1250. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
1251. qPCR, RT- Control - (reply: 2)
1252. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
1253. real time pcr melt curve and primer efficiency problem - (reply: 3)
1254. real time PCR - base line not zero anymore! (reply: 5)
1255. Pre-amplification of template for qPCR - (reply: 1)
1256. Efficiency estimation using a window of linearity - (reply: 3)
1257. Software for analysis of raw real-time flouresence data - Analysis of tab delimited text flouresence v cycle data (reply: 7)
1258. Real time PCR - Standard curve construction (reply: 2)
1259. issue with taqman MGB probes - (reply: 2)
1260. PCR product dimer issue - (reply: 24)