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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
481. iCycler Calibration Question - (reply: 1)
482. Long PCR (>10kb) polymerase recommendations - (reply: 3)
483. thermocycler - real-time versus conventional? - (reply: 2)
484. I need help for designing a pair of primers for this sequence. - (reply: 1)
485. Change in Superscript lot influences transcript level of one gene but not others - (reply: 2)
486. 1/delta Ct vs delta delat CT - (reply: 1)
487. PCR band slightly BELOW expected length ?! - (reply: 14)
488. Does anyone have any problem with Quanta Sybr Green - (reply: 2)
489. Trouble correlating GU/ml qPCR results to cfu/ml plating results - (reply: 2)
490. long primers PCR - (reply: 4)
491. unexpected band in PCR with plasmid - (reply: 4)
492. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
493. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
494. DNA extraction - PCR Problem - (reply: 3)
495. Real Time Trouble, low cTs - (reply: 3)
496. interpretation of results - (reply: 8)
497. Multiple Bands in qPCR - (reply: 4)
498. PCR product biotinylation - (reply: 3)
499. Tagged primers and a second PCR targetting the tags - (reply: 5)
500. Question about isolating DNA from the blood sample - (reply: 2)
501. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
502. Universal ProbeLibrary for Human by ROCHE - (reply: 1)
503. role of water in PCR - (reply: 3)
504. minimum amount of RNA for RT_PCR - (reply: 1)
505. Is it possible to compare abundance of isoforms by qPCR - (reply: 6)
506. HIV gene- PCR - (reply: 3)
507. Real time PCR melting curve - (reply: 2)
508. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
509. Normalization to Standard Gene: what does this mean - (reply: 8)
510. isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)