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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
481. Homogenize pancreas for RNA extraction - (reply: 3)
482. Point mutagenesis..... - (reply: 6)
483. No bands in PCR after DNase treatment - (reply: 4)
484. Overlap PCR problem - (reply: 5)
485. PCR product purification - (reply: 4)
486. Overlapping sequence PCR primers - (reply: 1)
487. acceptable melt curve? - (reply: 1)
488. Calibrator sample - (reply: 4)
489. Ct value very high. What can I do to improve this ct? - (reply: 4)
490. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
491. Amount of RNA (ng) for good ct values. - (reply: 3)
492. Normalizing qPCR data - (reply: 1)
493. doing PCR using PCR product , help plz !! - (reply: 5)
494. High GC content RT-qPCR - please help - (reply: 3)
495. Electrophoresis problem , please help pals ! - (reply: 8)
496. Calculating Standard Errors and Normalising to Transgene - (reply: 1)
497. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
498. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
499. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
500. iCycler Calibration Question - (reply: 1)
501. Long PCR (>10kb) polymerase recommendations - (reply: 3)
502. thermocycler - real-time versus conventional? - (reply: 2)
503. I need help for designing a pair of primers for this sequence. - (reply: 1)
504. Change in Superscript lot influences transcript level of one gene but not others - (reply: 2)
505. 1/delta Ct vs delta delat CT - (reply: 1)
506. PCR band slightly BELOW expected length ?! - (reply: 14)
507. Does anyone have any problem with Quanta Sybr Green - (reply: 2)
508. Trouble correlating GU/ml qPCR results to cfu/ml plating results - (reply: 2)
509. long primers PCR - (reply: 4)
510. unexpected band in PCR with plasmid - (reply: 4)