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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
481. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
482. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
483. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
484. iCycler Calibration Question - (reply: 1)
485. Long PCR (>10kb) polymerase recommendations - (reply: 3)
486. thermocycler - real-time versus conventional? - (reply: 2)
487. I need help for designing a pair of primers for this sequence. - (reply: 1)
488. Change in Superscript lot influences transcript level of one gene but not others - (reply: 2)
489. 1/delta Ct vs delta delat CT - (reply: 1)
490. PCR band slightly BELOW expected length ?! - (reply: 14)
491. Does anyone have any problem with Quanta Sybr Green - (reply: 2)
492. Trouble correlating GU/ml qPCR results to cfu/ml plating results - (reply: 2)
493. long primers PCR - (reply: 4)
494. unexpected band in PCR with plasmid - (reply: 4)
495. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
496. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
497. DNA extraction - PCR Problem - (reply: 3)
498. Real Time Trouble, low cTs - (reply: 3)
499. interpretation of results - (reply: 8)
500. Multiple Bands in qPCR - (reply: 4)
501. PCR product biotinylation - (reply: 3)
502. Tagged primers and a second PCR targetting the tags - (reply: 5)
503. Question about isolating DNA from the blood sample - (reply: 2)
504. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
505. Universal ProbeLibrary for Human by ROCHE - (reply: 1)
506. role of water in PCR - (reply: 3)
507. minimum amount of RNA for RT_PCR - (reply: 1)
508. Is it possible to compare abundance of isoforms by qPCR - (reply: 6)
509. HIV gene- PCR - (reply: 3)
510. Real time PCR melting curve - (reply: 2)