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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
481. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
482. Negative Control Looks Like the Rest - (reply: 11)
483. Why RNase H treatment after cDNA synthesis - (reply: 2)
484. RNA isolation from Sesame seed - (reply: 3)
485. qPCR with Genomic DNA - (reply: 1)
486. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
487. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
488. Homogenize pancreas for RNA extraction - (reply: 3)
489. Point mutagenesis..... - (reply: 6)
490. No bands in PCR after DNase treatment - (reply: 4)
491. Overlap PCR problem - (reply: 5)
492. PCR product purification - (reply: 4)
493. Overlapping sequence PCR primers - (reply: 1)
494. acceptable melt curve? - (reply: 1)
495. Calibrator sample - (reply: 4)
496. Ct value very high. What can I do to improve this ct? - (reply: 4)
497. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
498. Amount of RNA (ng) for good ct values. - (reply: 3)
499. Normalizing qPCR data - (reply: 1)
500. doing PCR using PCR product , help plz !! - (reply: 5)
501. High GC content RT-qPCR - please help - (reply: 3)
502. Electrophoresis problem , please help pals ! - (reply: 8)
503. Calculating Standard Errors and Normalising to Transgene - (reply: 1)
504. add template first or Syber mix first to reduce contamination in qPCR? - (reply: 3)
505. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
506. PCR-screening of T-DNA inserted in Arabidopsis gene - (reply: 1)
507. iCycler Calibration Question - (reply: 1)
508. Long PCR (>10kb) polymerase recommendations - (reply: 3)
509. thermocycler - real-time versus conventional? - (reply: 2)
510. I need help for designing a pair of primers for this sequence. - (reply: 1)