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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
481. Controls for RT-PCR reactions - (reply: 2)
482. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
483. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
484. issues when designing PCR primers using PRIMER3 - (reply: 8)
485. Primer catalog/databasing - (reply: 1)
486. Nonrepetitive nested PCR - (reply: 2)
487. DNA or cDNA? - (reply: 1)
488. Difference in Ct value of replicates - (reply: 1)
489. TDNA Genotyping PCR program - (reply: 1)
490. Shift in peaks at dissociation curve - (reply: 4)
491. High resolution melting analysis-SNP genotyping. - (reply: 1)
492. PCR - (reply: 2)
493. nonspecific band at 50bp in PCR - (reply: 9)
494. cDNA sequencing - (reply: 2)
495. Maximum PCR product Tm - (reply: 6)
496. QuikChange with Different plasmids - (reply: 1)
497. Good melting curve but non-specific products appeared - (reply: 2)
498. Long Non-Specific PCR Products - (reply: 5)
499. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
500. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
501. fold change (how to represent it) - (reply: 1)
502. PCR - consistent false positive results - (reply: 6)
503. geNorm alternative for qPCR housekeeping genes - (reply: 1)
504. PCR no amplification - (reply: 15)
505. positive NTC in real time reaction - (reply: 12)
506. Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
507. How to quantify virus by PCR - (reply: 12)
508. qPCR contamination problem - (reply: 5)
509. Error rate of commecial reverse transcriptase - (reply: 1)
510. Why do i get DNA of low concentrate after purification with kit - (reply: 9)