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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
481. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
482. Normalization to Standard Gene: what does this mean - (reply: 8)
483. isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)
484. How to Join two PCR products - (reply: 3)
485. Reference RNA for qPCR - (reply: 3)
486. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
487. What's wrong with my PCR - (reply: 5)
488. What does 'prevention of nontemplate-directed nucleotide addition' mean? - (reply: 3)
489. loss or results (ABI prism 7000) - (reply: 6)
490. Ct values - (reply: 3)
491. Problem with UPL real-time PCR - please help !!! - (reply: 1)
492. qRT-PCR data analysis - (reply: 2)
493. Cp, but no amplification - (reply: 2)
494. Strange RT to qPCR results with new kit - (reply: 5)
495. Low PCR product - (reply: 7)
496. SYBR vs FAM probe - (reply: 1)
497. Quantify gDNA in buccal swab - (reply: 2)
498. qPCR: band in gel, but super high Cq? - (reply: 10)
499. Melt curve analysis - (reply: 5)
500. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
501. How to Compare CT values between treated and untreated bacteria with uneven grow - (reply: 1)
502. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
503. wrong dntp concentration - (reply: 2)
504. Which one is better as reference gene base on these data - (reply: 3)
505. About the qPCRs and statistics - (reply: 2)
506. long fragment PCR - (reply: 2)
507. Replacing one reagent by another in a protocol (Please help) - (reply: 5)
508. RT-qPCR normalization - (reply: 3)
509. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
510. I need to break this cycle of PCR issues - (reply: 13)