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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1531. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
1532. Lucicferase activity and endogenous transcription - (reply: 7)
1533. need help on primer calcuation soon - (reply: 1)
1534. Heart ache in real time - (reply: 1)
1535. HEX vs. Cy-5 - (reply: 3)
1536. Direct PCR (PCR without DNA extraction) - (reply: 2)
1537. PCR using xt5 - (reply: 2)
1538. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
1539. Problems with qRT-PCR - Lots of unspecific products (reply: 2)
1540. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
1541. 2 bands or smear? 2-step RT-PCR - (reply: 1)
1542. 23S rRNA Primer - need primer sequence (reply: 1)
1543. Lowered extension temps with Phusion - (reply: 7)
1544. RT-PCR - problems with TaqMan PCR (reply: 2)
1545. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
1546. Two annealing temps? - (reply: 4)
1547. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
1548. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
1549. Small product amplification problems - (reply: 4)
1550. ROX and Rotorgene - using a mix with reference dye in Rotorgene (reply: 2)
1551. Freezing primers+SYBR green - (reply: 2)
1552. Tagged Primers - (reply: 5)
1553. no pcr amplification product - (reply: 9)
1554. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
1555. less steep curve and high Ct value! - (reply: 2)
1556. Question about site directed mutagenesis - (reply: 1)
1557. Amplfication of fungal DNA - (reply: 8)
1558. PCR problem - (reply: 4)
1559. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
1560. differently behavioring replicates in real-time PCR - (reply: 1)