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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1531. DATA ANALYSIS for qPCR - Calculating 2^(-Del Del Ct). (reply: 2)
1532. 18s control in qPCR - (reply: 2)
1533. weird PCR ask for help - (reply: 12)
1534. Absolute quantification and normalization - Are reference genes necessary? (reply: 1)
1535. What's the best electronic pipette (Precision) - (reply: 5)
1536. No PCR product at all - (reply: 8)
1537. PCR troubleshoooooting - primer dimer (reply: 1)
1538. Primer efficiency test - (reply: 1)
1539. primer software - (reply: 2)
1540. Question on wired PCR - (reply: 1)
1541. Validation of housekeeping gene expression - (reply: 3)
1542. restriction digestion of PCR product - (reply: 7)
1543. reuse pipette tips - (reply: 2)
1544. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
1545. How to know in which exon a primer match? - (reply: 1)
1546. dUTPs in qRTPCR rxn - (reply: 1)
1547. Amplification of human genomic DNA - (reply: 2)
1548. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1549. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1550. make your own gotaq - (reply: 2)
1551. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1552. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1553. primer RE over-hang nucleotides - common sequences? (reply: 5)
1554. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1555. Does RT step smplify RNA - (reply: 2)
1556. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1557. ROX utilization - (who uses ROX?) (reply: 1)
1558. qPCR analysis - (reply: 6)
1559. good housekeeping for ovary - (reply: 2)
1560. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)