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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1531. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
1532. primer design problem - (reply: 1)
1533. The perfect RT??? which control? - (reply: 4)
1534. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
1535. qPCR - qPCR problems.. (reply: 5)
1536. is it necessary to do DNases treatment? - (reply: 2)
1537. problem with site directed mutagenesis - (reply: 4)
1538. allele specific pcr - determining genotypes (reply: 2)
1539. Low 260/230 ratio in my RNA - (reply: 3)
1540. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
1541. Problem with primer efficiency analysis - (reply: 4)
1542. calibrator - what is this in qPCR (reply: 1)
1543. slan real time PCR system for validation of microarray results? - (reply: 1)
1544. PCR unusualband at ~230bps - PCR (reply: 1)
1545. DMSO or BSA for PCR - (reply: 6)
1546. primer design - (reply: 1)
1547. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
1548. Help me answer this curious student - Many questions on techniques (reply: 1)
1549. Tris Buffer for PCR reaction - why? (reply: 1)
1550. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
1551. PCR a plasmid protein - (reply: 2)
1552. multiplex PCR - (reply: 4)
1553. Realtime PCR machine - (reply: 2)
1554. PCR set-up calculation nightmares. - (reply: 3)
1555. Primer desing - (reply: 2)
1556. minimum amount of cDNA? - molecular biology (reply: 6)
1557. Absolute quantification - (reply: 1)
1558. Fast and Furious: Multiplex PCR - Need expert's opinion and help---perhaps with a bit counseling (reply: 10)
1559. How to compare different expression levels between genes - (reply: 1)
1560. DNA contamination after RNA extraction, which kit works best? - (reply: 6)