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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1531. Two annealing temps? - (reply: 4)
1532. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
1533. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
1534. Small product amplification problems - (reply: 4)
1535. ROX and Rotorgene - using a mix with reference dye in Rotorgene (reply: 2)
1536. Freezing primers+SYBR green - (reply: 2)
1537. Tagged Primers - (reply: 5)
1538. no pcr amplification product - (reply: 9)
1539. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
1540. less steep curve and high Ct value! - (reply: 2)
1541. Question about site directed mutagenesis - (reply: 1)
1542. Amplfication of fungal DNA - (reply: 8)
1543. PCR problem - (reply: 4)
1544. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
1545. differently behavioring replicates in real-time PCR - (reply: 1)
1546. Multiplex PCR - (reply: 1)
1547. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1548. optimal template dna concentrations - (reply: 1)
1549. reconstitution of primers - (reply: 2)
1550. Mysterious PCR Contamination - (reply: 13)
1551. RT-PCR Help - Protocol provided (reply: 4)
1552. Endogenous control in bacterial RT-qPCR - (reply: 4)
1553. Mutagenesis PCR and undesired amplification - (reply: 2)
1554. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1555. primer dimer - (reply: 2)
1556. Genotyping conditioning-where to start? - (reply: 1)
1557. Marker migrates slower depending where is loaded - (reply: 2)
1558. PCR on degraded templates - (reply: 2)
1559. PCR help needed - (reply: 3)
1560. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)