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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1531. ROX and Rotorgene - using a mix with reference dye in Rotorgene (reply: 2)
1532. Freezing primers+SYBR green - (reply: 2)
1533. Tagged Primers - (reply: 5)
1534. no pcr amplification product - (reply: 9)
1535. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
1536. less steep curve and high Ct value! - (reply: 2)
1537. Question about site directed mutagenesis - (reply: 1)
1538. Amplfication of fungal DNA - (reply: 8)
1539. PCR problem - (reply: 4)
1540. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
1541. differently behavioring replicates in real-time PCR - (reply: 1)
1542. Multiplex PCR - (reply: 1)
1543. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1544. optimal template dna concentrations - (reply: 1)
1545. reconstitution of primers - (reply: 2)
1546. Mysterious PCR Contamination - (reply: 13)
1547. RT-PCR Help - Protocol provided (reply: 4)
1548. Endogenous control in bacterial RT-qPCR - (reply: 4)
1549. Mutagenesis PCR and undesired amplification - (reply: 2)
1550. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1551. primer dimer - (reply: 2)
1552. Genotyping conditioning-where to start? - (reply: 1)
1553. Marker migrates slower depending where is loaded - (reply: 2)
1554. PCR on degraded templates - (reply: 2)
1555. PCR help needed - (reply: 3)
1556. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
1557. Tm difference - (reply: 2)
1558. Genotyping - PCR not working (reply: 6)
1559. RT qPCR Newbie, Weird Results - (reply: 1)
1560. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)