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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1531. primer dimer - proplems (reply: 1)
1532. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
1533. DATA ANALYSIS for qPCR - Calculating 2^(-Del Del Ct). (reply: 2)
1534. 18s control in qPCR - (reply: 2)
1535. weird PCR ask for help - (reply: 12)
1536. Absolute quantification and normalization - Are reference genes necessary? (reply: 1)
1537. What's the best electronic pipette (Precision) - (reply: 5)
1538. No PCR product at all - (reply: 8)
1539. PCR troubleshoooooting - primer dimer (reply: 1)
1540. Primer efficiency test - (reply: 1)
1541. primer software - (reply: 2)
1542. Question on wired PCR - (reply: 1)
1543. Validation of housekeeping gene expression - (reply: 3)
1544. restriction digestion of PCR product - (reply: 7)
1545. reuse pipette tips - (reply: 2)
1546. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
1547. How to know in which exon a primer match? - (reply: 1)
1548. dUTPs in qRTPCR rxn - (reply: 1)
1549. Amplification of human genomic DNA - (reply: 2)
1550. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1551. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1552. make your own gotaq - (reply: 2)
1553. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1554. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1555. primer RE over-hang nucleotides - common sequences? (reply: 5)
1556. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1557. Does RT step smplify RNA - (reply: 2)
1558. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1559. ROX utilization - (who uses ROX?) (reply: 1)
1560. qPCR analysis - (reply: 6)