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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1531. dUTPs in qRTPCR rxn - (reply: 1)
1532. Amplification of human genomic DNA - (reply: 2)
1533. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1534. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1535. make your own gotaq - (reply: 2)
1536. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1537. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1538. primer RE over-hang nucleotides - common sequences? (reply: 5)
1539. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1540. Does RT step smplify RNA - (reply: 2)
1541. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1542. ROX utilization - (who uses ROX?) (reply: 1)
1543. qPCR analysis - (reply: 6)
1544. good housekeeping for ovary - (reply: 2)
1545. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
1546. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
1547. primer design problem - (reply: 1)
1548. The perfect RT??? which control? - (reply: 4)
1549. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
1550. qPCR - qPCR problems.. (reply: 5)
1551. is it necessary to do DNases treatment? - (reply: 2)
1552. problem with site directed mutagenesis - (reply: 4)
1553. allele specific pcr - determining genotypes (reply: 2)
1554. Low 260/230 ratio in my RNA - (reply: 3)
1555. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
1556. Problem with primer efficiency analysis - (reply: 4)
1557. calibrator - what is this in qPCR (reply: 1)
1558. slan real time PCR system for validation of microarray results? - (reply: 1)
1559. PCR unusualband at ~230bps - PCR (reply: 1)
1560. DMSO or BSA for PCR - (reply: 6)