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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1531. Reference gene qPCR - (reply: 4)
1532. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
1533. Real time PCR info - (reply: 4)
1534. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
1535. standard curve - (reply: 2)
1536. PCR from BAC - PCR from BAC (reply: 1)
1537. Mean versus Mediat Ct Values - Which to use for statistical analsyis (reply: 1)
1538. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
1539. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
1540. heating rna before rt reaction? - (reply: 1)
1541. relative gene expression with multiple reference genes - data analysis (reply: 1)
1542. qRT-PCR no of cycles?? - (reply: 6)
1543. Is there any setting to avoid same base runs in Primer-BLAST? - (reply: 3)
1544. Which techniques should i do? - Which techniques should i do? (reply: 2)
1545. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
1546. what primers should I use for DNA sequencing? please help - (reply: 2)
1547. Standard curve for RT-PCR - (reply: 1)
1548. Problem amplifying plasmid - (reply: 1)
1549. What Factors Affect Ct values of a gene? - (reply: 1)
1550. Sybr Green I in real-time PCR reaction - (reply: 3)
1551. Primers annealing temperatures - (reply: 8)
1552. Difficulties cloning by PCR - (reply: 5)
1553. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
1554. rna storage at -20 - (reply: 2)
1555. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
1556. high Cp value PCR - (reply: 2)
1557. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
1558. Synthesize own Oligo-dT primers - (reply: 6)
1559. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
1560. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)