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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1531. Fast and Furious: Multiplex PCR - Need expert's opinion and help---perhaps with a bit counseling (reply: 10)
1532. How to compare different expression levels between genes - (reply: 1)
1533. DNA contamination after RNA extraction, which kit works best? - (reply: 6)
1534. primer-blast from ncbi - (reply: 3)
1535. Page or HPLC primers - What is the difference (reply: 2)
1536. Standard curves for PCR efficiency. - (reply: 2)
1537. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
1538. Looking for help with my PCR! - (reply: 3)
1539. HKG validation - interplate normalisation (reply: 2)
1540. PCR without thermal cycler? - (reply: 9)
1541. primer contamination - primer contaminated with ice (reply: 3)
1542. Blue Juice - I think I need to redo... (reply: 3)
1543. Loss of volume in PCR reaction in 96 well plate - (reply: 7)
1544. RT-PCR problem - (reply: 4)
1545. home made sybr green mix for qpcr - (reply: 2)
1546. reference genes for qPCR - (reply: 3)
1547. PCR Efficiency - (reply: 2)
1548. DNA and RNA contamination in RT PCR water controls - (reply: 2)
1549. Optimal target length - (reply: 3)
1550. Fermentas SYBR Green vs. Qiagen SYBR Green - (reply: 2)
1551. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
1552. Different types/Brands of BSA? - (reply: 2)
1553. NTC with specific amplification - (reply: 1)
1554. Lost a Band by changing thermocycler - (reply: 9)
1555. Primer3 vs Primer BLAST - (reply: 3)
1556. qPCR using standard curve method - how to do correct setup? (reply: 3)
1557. Primer-Blast - (reply: 3)
1558. Amplification in NTC and noRT controls - (reply: 5)
1559. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
1560. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)