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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
151. Degenerated Primers and their problems - (reply: 1)
152. Interpreting primer BLAST scores for self-complementary - (reply: 5)
153. Testing primers on 'unknown' tissue - (reply: 1)
154. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
155. nested PCR for low viral load- HBV patient sample - (reply: 2)
156. Finding an answer as to why I have Tm but no Ct - (reply: 6)
157. Amplification in HK gene but not for target gene - (reply: 3)
158. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
159. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
160. how to make this reaction? - (reply: 1)
161. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
162. How long can I store at - 30C my PCR mix? - (reply: 6)
163. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
164. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
165. RT-PCR help - (reply: 2)
166. Strange signals in the NTC - (reply: 1)
167. False positive - help - (reply: 3)
168. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
169. PCR Temperature change control malfunctioning - (reply: 1)
170. Assay question - (reply: 1)
171. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
172. HRM instrumentaion - (reply: 4)
173. Can you repeat a thermocycle? - (reply: 3)
174. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
175. Amplification curve in the negative control samples - (reply: 7)
176. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
177. Primer as limiting reagent in PCR reaction - (reply: 2)
178. Taqman probe in a LightCycler 480? - (reply: 1)
179. Real time PCR for degraded RNA - (reply: 1)
180. Ct value of housekeeping gene - (reply: 4)