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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
151. transcript variant X - (reply: 5)
152. RNA extraction for RT-PCR - (reply: 3)
153. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
154. problems with gDNA doing real time PCR in yeast - (reply: 2)
155. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
156. hybridization probe - (reply: 1)
157. How to get rid of bands in PCR negative control - (reply: 10)
158. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
159. standard curve using genomic dna - (reply: 1)
160. PCR problem - (reply: 2)
161. defining ratio of alternative spliced gene with qPCR - (reply: 14)
162. Poor efficiency standard curve - (reply: 2)
163. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
164. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
165. PCR gene specific amplification problem - (reply: 3)
166. Failure SYBRGREEN PCR - (reply: 4)
167. Pfaffl Method for relative - (reply: 4)
168. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
169. experiences with NuPCR from Illumina? - (reply: 1)
170. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
171. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
172. 100 ng RNA for cDNA synthesis - (reply: 4)
173. Inexplicable qPCR failure in single wells - (reply: 8)
174. negative control well 300 bp band - (reply: 3)
175. Reference gene for normalisation - for different growth rates - (reply: 3)
176. Strange amplification plots with high Ct variability - (reply: 1)
177. How big a role does mixing play in PCR - (reply: 1)
178. Melting curve is irregular for primer optimization - (reply: 5)
179. Designing primers for ABO blood groups - (reply: 1)
180. Methylight Results Analysis - (reply: 1)