Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
151. PCR Temperature change control malfunctioning - (reply: 1)
152. Assay question - (reply: 1)
153. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
154. HRM instrumentaion - (reply: 4)
155. Can you repeat a thermocycle? - (reply: 3)
156. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
157. Amplification curve in the negative control samples - (reply: 7)
158. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
159. Primer as limiting reagent in PCR reaction - (reply: 2)
160. Taqman probe in a LightCycler 480? - (reply: 1)
161. Real time PCR for degraded RNA - (reply: 1)
162. Ct value of housekeeping gene - (reply: 4)
163. Efficiency correct fold change -- use all or none? - (reply: 1)
164. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
165. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
166. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
167. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
168. Is this primer-dimer peak in my melting curve? - (reply: 6)
169. Taq / Phusion mix - (reply: 3)
170. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
171. Dilution series between HKG and GOI - (reply: 7)
172. Reference gene(s) selection and validaton for qPCR - (reply: 3)
173. Is that normal the negative control showing signals? - (reply: 13)
174. qPCR cannot detect reaction. - (reply: 1)
175. website for primer design - (reply: 1)
176. PCR Efficiency over 150%! - (reply: 1)
177. problem of amplification - (reply: 2)
178. Rxn not working - amplify/linearize plasmid - (reply: 3)
179. qRT-PCR works one day, and not the next - (reply: 1)
180. PCR not working - (reply: 11)