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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
151. Calculation of Taq Polymerase - (reply: 3)
152. Best Taq for colony PCR? - (reply: 5)
153. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
154. How to test my milleq water in q PCR about contaminants - (reply: 3)
155. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
156. real time PCR primer design - (reply: 1)
157. Plasmid Linearization Problems - (reply: 9)
158. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
159. Increasing RNA yield from very small tissues - (reply: 3)
160. Degenerated Primers and their problems - (reply: 1)
161. Interpreting primer BLAST scores for self-complementary - (reply: 5)
162. Testing primers on 'unknown' tissue - (reply: 1)
163. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
164. nested PCR for low viral load- HBV patient sample - (reply: 2)
165. Finding an answer as to why I have Tm but no Ct - (reply: 6)
166. Amplification in HK gene but not for target gene - (reply: 3)
167. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
168. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
169. how to make this reaction? - (reply: 1)
170. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
171. How long can I store at - 30C my PCR mix? - (reply: 6)
172. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
173. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
174. RT-PCR help - (reply: 2)
175. Strange signals in the NTC - (reply: 1)
176. False positive - help - (reply: 3)
177. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
178. PCR Temperature change control malfunctioning - (reply: 1)
179. Assay question - (reply: 1)
180. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)