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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
151. Best Taq for colony PCR? - (reply: 5)
152. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
153. How to test my milleq water in q PCR about contaminants - (reply: 3)
154. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
155. real time PCR primer design - (reply: 1)
156. Plasmid Linearization Problems - (reply: 9)
157. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
158. Increasing RNA yield from very small tissues - (reply: 3)
159. Degenerated Primers and their problems - (reply: 1)
160. Interpreting primer BLAST scores for self-complementary - (reply: 5)
161. Testing primers on 'unknown' tissue - (reply: 1)
162. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
163. nested PCR for low viral load- HBV patient sample - (reply: 2)
164. Finding an answer as to why I have Tm but no Ct - (reply: 6)
165. Amplification in HK gene but not for target gene - (reply: 3)
166. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
167. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
168. how to make this reaction? - (reply: 1)
169. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
170. How long can I store at - 30C my PCR mix? - (reply: 6)
171. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
172. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
173. RT-PCR help - (reply: 2)
174. Strange signals in the NTC - (reply: 1)
175. False positive - help - (reply: 3)
176. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
177. PCR Temperature change control malfunctioning - (reply: 1)
178. Assay question - (reply: 1)
179. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
180. HRM instrumentaion - (reply: 4)