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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
151. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
152. How long can I store at - 30C my PCR mix? - (reply: 6)
153. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
154. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
155. RT-PCR help - (reply: 2)
156. Strange signals in the NTC - (reply: 1)
157. False positive - help - (reply: 3)
158. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
159. PCR Temperature change control malfunctioning - (reply: 1)
160. Assay question - (reply: 1)
161. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
162. HRM instrumentaion - (reply: 4)
163. Can you repeat a thermocycle? - (reply: 3)
164. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
165. Amplification curve in the negative control samples - (reply: 7)
166. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
167. Primer as limiting reagent in PCR reaction - (reply: 2)
168. Taqman probe in a LightCycler 480? - (reply: 1)
169. Real time PCR for degraded RNA - (reply: 1)
170. Ct value of housekeeping gene - (reply: 4)
171. Efficiency correct fold change -- use all or none? - (reply: 1)
172. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
173. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
174. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
175. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
176. Is this primer-dimer peak in my melting curve? - (reply: 6)
177. Taq / Phusion mix - (reply: 3)
178. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
179. Dilution series between HKG and GOI - (reply: 7)
180. Reference gene(s) selection and validaton for qPCR - (reply: 5)