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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
151. Interpreting primer BLAST scores for self-complementary - (reply: 5)
152. Testing primers on 'unknown' tissue - (reply: 1)
153. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
154. nested PCR for low viral load- HBV patient sample - (reply: 2)
155. Finding an answer as to why I have Tm but no Ct - (reply: 6)
156. Amplification in HK gene but not for target gene - (reply: 3)
157. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
158. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
159. how to make this reaction? - (reply: 1)
160. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
161. How long can I store at - 30C my PCR mix? - (reply: 6)
162. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
163. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
164. RT-PCR help - (reply: 2)
165. Strange signals in the NTC - (reply: 1)
166. False positive - help - (reply: 3)
167. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
168. PCR Temperature change control malfunctioning - (reply: 1)
169. Assay question - (reply: 1)
170. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
171. HRM instrumentaion - (reply: 4)
172. Can you repeat a thermocycle? - (reply: 3)
173. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
174. Amplification curve in the negative control samples - (reply: 7)
175. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
176. Primer as limiting reagent in PCR reaction - (reply: 2)
177. Taqman probe in a LightCycler 480? - (reply: 1)
178. Real time PCR for degraded RNA - (reply: 1)
179. Ct value of housekeeping gene - (reply: 4)
180. Efficiency correct fold change -- use all or none? - (reply: 1)