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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
151. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
152. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
153. Is this primer-dimer peak in my melting curve? - (reply: 6)
154. Taq / Phusion mix - (reply: 3)
155. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
156. Dilution series between HKG and GOI - (reply: 7)
157. Reference gene(s) selection and validaton for qPCR - (reply: 3)
158. Is that normal the negative control showing signals? - (reply: 13)
159. qPCR cannot detect reaction. - (reply: 1)
160. website for primer design - (reply: 1)
161. PCR Efficiency over 150%! - (reply: 1)
162. problem of amplification - (reply: 2)
163. Rxn not working - amplify/linearize plasmid - (reply: 3)
164. qRT-PCR works one day, and not the next - (reply: 1)
165. PCR not working - (reply: 11)
166. an urgent problem with replicates - (reply: 10)
167. Site-directed mutagenesis: maximum substitution - (reply: 1)
168. Inter run calibration qRT-PCR - (reply: 2)
169. Primer design and alternative transcripts - (reply: 2)
170. qPCR amplification - (reply: 4)
171. Extremely desperate noob question: How do these PCR work? - (reply: 6)
172. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
173. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
174. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
175. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
176. Genomic DNA Isolation from Soft Agar Colonies - (reply: 3)
177. help with total RNA isolation - (reply: 1)
178. Need help to identify bands in AGE result - (reply: 3)
179. COLD-PCR (Dissociation Curve) via qPCR - (reply: 4)
180. how to hasten real-time PCR amplifications - (reply: 2)