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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
151. haeting RNA before electrophoresis - (reply: 1)
152. PCR Products not run well in the GEL - (reply: 1)
153. Qubit Nanodrop Quantifications - (reply: 1)
154. Decontaminating DNA - Effective Means? - (reply: 4)
155. Consistancy issue with PCR block - (reply: 1)
156. Queries regarding PCR - (reply: 3)
157. Identification of Listeria monocytogenes - (reply: 1)
158. Calculation of Taq Polymerase - (reply: 3)
159. Best Taq for colony PCR? - (reply: 5)
160. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
161. How to test my milleq water in q PCR about contaminants - (reply: 3)
162. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
163. real time PCR primer design - (reply: 1)
164. Plasmid Linearization Problems - (reply: 9)
165. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
166. Increasing RNA yield from very small tissues - (reply: 3)
167. Degenerated Primers and their problems - (reply: 1)
168. Interpreting primer BLAST scores for self-complementary - (reply: 5)
169. Testing primers on 'unknown' tissue - (reply: 1)
170. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
171. nested PCR for low viral load- HBV patient sample - (reply: 2)
172. Finding an answer as to why I have Tm but no Ct - (reply: 6)
173. Amplification in HK gene but not for target gene - (reply: 3)
174. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
175. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
176. how to make this reaction? - (reply: 1)
177. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
178. How long can I store at - 30C my PCR mix? - (reply: 6)
179. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
180. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)