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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
151. How to test my milleq water in q PCR about contaminants - (reply: 3)
152. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
153. real time PCR primer design - (reply: 1)
154. Plasmid Linearization Problems - (reply: 9)
155. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
156. Increasing RNA yield from very small tissues - (reply: 3)
157. Degenerated Primers and their problems - (reply: 1)
158. Interpreting primer BLAST scores for self-complementary - (reply: 5)
159. Testing primers on 'unknown' tissue - (reply: 1)
160. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
161. nested PCR for low viral load- HBV patient sample - (reply: 2)
162. Finding an answer as to why I have Tm but no Ct - (reply: 6)
163. Amplification in HK gene but not for target gene - (reply: 3)
164. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
165. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
166. how to make this reaction? - (reply: 1)
167. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
168. How long can I store at - 30C my PCR mix? - (reply: 6)
169. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
170. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
171. RT-PCR help - (reply: 2)
172. Strange signals in the NTC - (reply: 1)
173. False positive - help - (reply: 3)
174. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
175. PCR Temperature change control malfunctioning - (reply: 1)
176. Assay question - (reply: 1)
177. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
178. HRM instrumentaion - (reply: 4)
179. Can you repeat a thermocycle? - (reply: 3)
180. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)