Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
151. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
152. Increasing RNA yield from very small tissues - (reply: 3)
153. Degenerated Primers and their problems - (reply: 1)
154. Interpreting primer BLAST scores for self-complementary - (reply: 5)
155. Testing primers on 'unknown' tissue - (reply: 1)
156. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
157. nested PCR for low viral load- HBV patient sample - (reply: 2)
158. Finding an answer as to why I have Tm but no Ct - (reply: 6)
159. Amplification in HK gene but not for target gene - (reply: 3)
160. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
161. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
162. how to make this reaction? - (reply: 1)
163. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
164. How long can I store at - 30C my PCR mix? - (reply: 6)
165. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
166. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
167. RT-PCR help - (reply: 2)
168. Strange signals in the NTC - (reply: 1)
169. False positive - help - (reply: 3)
170. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
171. PCR Temperature change control malfunctioning - (reply: 1)
172. Assay question - (reply: 1)
173. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
174. HRM instrumentaion - (reply: 4)
175. Can you repeat a thermocycle? - (reply: 3)
176. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
177. Amplification curve in the negative control samples - (reply: 7)
178. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
179. Primer as limiting reagent in PCR reaction - (reply: 2)
180. Taqman probe in a LightCycler 480? - (reply: 1)