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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1771. PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
1772. failed PCR on DNA extract from blood - (reply: 6)
1773. Alternative to netprimer - (reply: 5)
1774. Will ligase buffer affect polymerase fidelity? - (reply: 2)
1775. Primer dimer issue in real time PCR - (reply: 21)
1776. Leaky RT-PCR - (reply: 4)
1777. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
1778. expressing data with more than 1 HK gene - (reply: 2)
1779. RT-PCR primer dimers and cDNA degradation - (reply: 2)
1780. Sequencing of scarce real-time amplicons - (reply: 1)
1781. PCR efficiency important in real time absolute quantification? - (reply: 4)
1782. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
1783. Genotyping PCR - (reply: 3)
1784. Is this standard valid? - pcr product as standards (reply: 5)
1785. geNorm NF calculation - normalization in geNorm (reply: 5)
1786. primer binding - (reply: 1)
1787. Real time 70% efficiency and DDCt - (reply: 1)
1788. RNA visualization - (reply: 1)
1789. PCR primers - (reply: 1)
1790. amplification in negative control in RT PCR - (reply: 2)
1791. 18S changes with cDNA dilution - (reply: 2)
1792. Deletion PCR - (reply: 9)
1793. crossing point - crossing point (reply: 2)
1794. Smear problem with my new primer set - (reply: 2)
1795. PCR ghost bands - (reply: 1)
1796. wash buffer for columns - how to make it? (reply: 3)
1797. amplicon storage - amplicon storage (reply: 3)
1798. CP of real time PCR by LC480 - (reply: 1)
1799. transient expression - best time to detect the mRNA? (reply: 1)
1800. Chosing the best housekeeping gene! - (reply: 5)