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91. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
92. standard curve using genomic dna - (reply: 1)
93. PCR problem - (reply: 2)
94. defining ratio of alternative spliced gene with qPCR - (reply: 13)
95. Poor efficiency standard curve - (reply: 2)
96. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
97. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
98. PCR gene specific amplification problem - (reply: 3)
99. Failure SYBRGREEN PCR - (reply: 4)
100. Pfaffl Method for relative - (reply: 4)
101. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
102. experiences with NuPCR from Illumina? - (reply: 1)
103. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
104. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
105. 100 ng RNA for cDNA synthesis - (reply: 4)
106. Inexplicable qPCR failure in single wells - (reply: 7)
107. negative control well 300 bp band - (reply: 3)
108. Reference gene for normalisation - for different growth rates - (reply: 3)
109. Strange amplification plots with high Ct variability - (reply: 1)
110. How big a role does mixing play in PCR - (reply: 1)
111. Melting curve is irregular for primer optimization - (reply: 5)
112. Designing primers for ABO blood groups - (reply: 1)
113. Methylight Results Analysis - (reply: 1)
114. GAPDH Ct value - (reply: 4)
115. dissociation curves - (reply: 1)
116. RT-PCR primer design - (reply: 7)
117. How to amplify very short PCR template - (reply: 4)
118. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
119. Is this primer okay? - (reply: 4)
120. Dilution calculation - (reply: 3)
92. standard curve using genomic dna - (reply: 1)
93. PCR problem - (reply: 2)
94. defining ratio of alternative spliced gene with qPCR - (reply: 13)
95. Poor efficiency standard curve - (reply: 2)
96. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
97. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
98. PCR gene specific amplification problem - (reply: 3)
99. Failure SYBRGREEN PCR - (reply: 4)
100. Pfaffl Method for relative - (reply: 4)
101. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
102. experiences with NuPCR from Illumina? - (reply: 1)
103. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
104. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
105. 100 ng RNA for cDNA synthesis - (reply: 4)
106. Inexplicable qPCR failure in single wells - (reply: 7)
107. negative control well 300 bp band - (reply: 3)
108. Reference gene for normalisation - for different growth rates - (reply: 3)
109. Strange amplification plots with high Ct variability - (reply: 1)
110. How big a role does mixing play in PCR - (reply: 1)
111. Melting curve is irregular for primer optimization - (reply: 5)
112. Designing primers for ABO blood groups - (reply: 1)
113. Methylight Results Analysis - (reply: 1)
114. GAPDH Ct value - (reply: 4)
115. dissociation curves - (reply: 1)
116. RT-PCR primer design - (reply: 7)
117. How to amplify very short PCR template - (reply: 4)
118. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
119. Is this primer okay? - (reply: 4)
120. Dilution calculation - (reply: 3)