Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
91. Amount of RNA for cDNA - (reply: 4)
92. Difference in melting temperature - (reply: 5)
93. Changing PCR recipe halfway - (reply: 1)
94. PCR master mix containing primers and Taq - (reply: 4)
95. Primer working with some cDNA samples and not other - (reply: 2)
96. no bands after pcr!!!! - (reply: 4)
97. Help with definitons for qPCR - (reply: 1)
98. Calibrator in qPCR - (reply: 7)
99. Experimental design for RT-PCR - (reply: 8)
100. Primer dilution - (reply: 7)
101. Calculation help - (reply: 6)
102. standards for qPCR and some other doubts - (reply: 9)
103. Plasma cortisol/corticosterone level analysis using qPCR - (reply: 1)
104. Technical Replicates vs Biological Replicates - (reply: 1)
105. pcr - (reply: 1)
106. Difference in synthesis - (reply: 1)
107. Standardizing cDNA concentration before doing PCR - (reply: 4)
108. Agarose Gel Electrophoresis - (reply: 1)
109. Volume of Primers - (reply: 4)
110. qPCR with different genes - (reply: 5)
111. PCR conditions unknown? - (reply: 10)
112. PCR anomaly in a mid-range dilution of the template - (reply: 2)
113. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
114. 2 peaks for identical sequences in HRM - (reply: 2)
115. PCR using very long oligos !!! - (reply: 4)
116. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
117. How to optimize a master mix kit? - (reply: 7)
118. pcr primer design - (reply: 6)
119. Please help (primer/ probe final volume calculations) - (reply: 1)
120. Primer design with EcoRI ends - (reply: 2)