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91. Sec. structure in primers - (reply: 1)
92. Differences in shape of melt curve - (reply: 4)
93. Amount of RNA for cDNA - (reply: 4)
94. Difference in melting temperature - (reply: 5)
95. Changing PCR recipe halfway - (reply: 1)
96. PCR master mix containing primers and Taq - (reply: 4)
97. Primer working with some cDNA samples and not other - (reply: 2)
98. no bands after pcr!!!! - (reply: 4)
99. Help with definitons for qPCR - (reply: 1)
100. Calibrator in qPCR - (reply: 7)
101. Experimental design for RT-PCR - (reply: 8)
102. Primer dilution - (reply: 7)
103. Calculation help - (reply: 6)
104. standards for qPCR and some other doubts - (reply: 9)
105. Plasma cortisol/corticosterone level analysis using qPCR - (reply: 1)
106. Technical Replicates vs Biological Replicates - (reply: 1)
107. pcr - (reply: 1)
108. Difference in synthesis - (reply: 1)
109. Standardizing cDNA concentration before doing PCR - (reply: 4)
110. Agarose Gel Electrophoresis - (reply: 1)
111. Volume of Primers - (reply: 4)
112. qPCR with different genes - (reply: 5)
113. PCR conditions unknown? - (reply: 10)
114. PCR anomaly in a mid-range dilution of the template - (reply: 2)
115. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
116. 2 peaks for identical sequences in HRM - (reply: 2)
117. PCR using very long oligos !!! - (reply: 4)
118. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
119. How to optimize a master mix kit? - (reply: 7)
120. pcr primer design - (reply: 6)
92. Differences in shape of melt curve - (reply: 4)
93. Amount of RNA for cDNA - (reply: 4)
94. Difference in melting temperature - (reply: 5)
95. Changing PCR recipe halfway - (reply: 1)
96. PCR master mix containing primers and Taq - (reply: 4)
97. Primer working with some cDNA samples and not other - (reply: 2)
98. no bands after pcr!!!! - (reply: 4)
99. Help with definitons for qPCR - (reply: 1)
100. Calibrator in qPCR - (reply: 7)
101. Experimental design for RT-PCR - (reply: 8)
102. Primer dilution - (reply: 7)
103. Calculation help - (reply: 6)
104. standards for qPCR and some other doubts - (reply: 9)
105. Plasma cortisol/corticosterone level analysis using qPCR - (reply: 1)
106. Technical Replicates vs Biological Replicates - (reply: 1)
107. pcr - (reply: 1)
108. Difference in synthesis - (reply: 1)
109. Standardizing cDNA concentration before doing PCR - (reply: 4)
110. Agarose Gel Electrophoresis - (reply: 1)
111. Volume of Primers - (reply: 4)
112. qPCR with different genes - (reply: 5)
113. PCR conditions unknown? - (reply: 10)
114. PCR anomaly in a mid-range dilution of the template - (reply: 2)
115. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
116. 2 peaks for identical sequences in HRM - (reply: 2)
117. PCR using very long oligos !!! - (reply: 4)
118. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
119. How to optimize a master mix kit? - (reply: 7)
120. pcr primer design - (reply: 6)