Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
91. No bands despite different primers and conditions and TAqs - (reply: 8)
92. Multiplex TaqMan-like Assay PCR Efficiency - (reply: 3)
93. Wrong PCR product - (reply: 2)
94. Primer design - (reply: 5)
95. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
96. Quantitative RT-PCR statistics help - (reply: 1)
97. PCR Primer trouble - (reply: 2)
98. I don't find housekeeping genes: what should I do? - (reply: 6)
99. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
100. excess rna sample affect qpcr? - (reply: 2)
101. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
102. finding the corrosponding primers - (reply: 2)
103. Gene sequence for Real time PCR - (reply: 2)
104. qPCR result of pooled samples - (reply: 1)
105. transcript variant X - (reply: 5)
106. RNA extraction for RT-PCR - (reply: 3)
107. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
108. problems with gDNA doing real time PCR in yeast - (reply: 2)
109. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
110. hybridization probe - (reply: 1)
111. How to get rid of bands in PCR negative control - (reply: 10)
112. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
113. standard curve using genomic dna - (reply: 1)
114. PCR problem - (reply: 2)
115. defining ratio of alternative spliced gene with qPCR - (reply: 13)
116. Poor efficiency standard curve - (reply: 2)
117. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
118. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
119. PCR gene specific amplification problem - (reply: 3)
120. Failure SYBRGREEN PCR - (reply: 4)