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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
91. Primer as limiting reagent in PCR reaction - (reply: 2)
92. Taqman probe in a LightCycler 480? - (reply: 1)
93. Real time PCR for degraded RNA - (reply: 1)
94. Ct value of housekeeping gene - (reply: 4)
95. Efficiency correct fold change -- use all or none? - (reply: 1)
96. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
97. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
98. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
99. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
100. Is this primer-dimer peak in my melting curve? - (reply: 6)
101. Taq / Phusion mix - (reply: 3)
102. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
103. Dilution series between HKG and GOI - (reply: 7)
104. Reference gene(s) selection and validaton for qPCR - (reply: 2)
105. Is that normal the negative control showing signals? - (reply: 13)
106. qPCR cannot detect reaction. - (reply: 1)
107. website for primer design - (reply: 1)
108. PCR Efficiency over 150%! - (reply: 1)
109. problem of amplification - (reply: 2)
110. Rxn not working - amplify/linearize plasmid - (reply: 3)
111. qRT-PCR works one day, and not the next - (reply: 1)
112. PCR not working - (reply: 11)
113. an urgent problem with replicates - (reply: 10)
114. Site-directed mutagenesis: maximum substitution - (reply: 1)
115. Inter run calibration qRT-PCR - (reply: 2)
116. Primer design and alternative transcripts - (reply: 2)
117. qPCR amplification - (reply: 4)
118. Extremely desperate noob question: How do these PCR work? - (reply: 6)
119. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
120. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)