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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
91. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
92. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
93. RT-PCR help - (reply: 2)
94. Strange signals in the NTC - (reply: 1)
95. False positive - help - (reply: 3)
96. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
97. PCR Temperature change control malfunctioning - (reply: 1)
98. Assay question - (reply: 1)
99. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
100. HRM instrumentaion - (reply: 4)
101. Can you repeat a thermocycle? - (reply: 3)
102. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
103. Amplification curve in the negative control samples - (reply: 7)
104. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
105. Primer as limiting reagent in PCR reaction - (reply: 2)
106. Taqman probe in a LightCycler 480? - (reply: 1)
107. Real time PCR for degraded RNA - (reply: 1)
108. Ct value of housekeeping gene - (reply: 4)
109. Efficiency correct fold change -- use all or none? - (reply: 1)
110. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
111. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
112. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
113. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
114. Is this primer-dimer peak in my melting curve? - (reply: 6)
115. Taq / Phusion mix - (reply: 3)
116. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
117. Dilution series between HKG and GOI - (reply: 7)
118. Reference gene(s) selection and validaton for qPCR - (reply: 2)
119. Is that normal the negative control showing signals? - (reply: 13)
120. qPCR cannot detect reaction. - (reply: 1)