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91. something very basic, why called "event-specific detection" - (reply: 1)
92. skip 65 degrees protocol of Reverse transcription - (reply: 4)
93. Mystery in my PCR - (reply: 5)
94. Whole genome amplification from cDNA? - (reply: 4)
95. What do you use to check primer secondary structure? - (reply: 3)
96. CT of qPCR - (reply: 3)
97. Need help with my real time RT-PCR Plate Set up - (reply: 8)
98. PCR product as template for in vitro transcription - (reply: 1)
99. Sybr Green vs Taqman -- a practical approach - (reply: 2)
100. Problem with 3 step PCR - (reply: 3)
101. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
102. ARMS-PCR - (reply: 1)
103. Scaling up PCR to get more DNA - (reply: 5)
104. RNA concentration suddenly drops - (reply: 3)
105. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
106. PCR to get 10kbp product - (reply: 4)
107. site-directed mutagenesis primer Tm - (reply: 4)
108. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
109. RNA concentration to start RT reaction - (reply: 1)
110. Random vs oligo primer in preamplification RT - (reply: 1)
111. Smart Cycler II software and Absolute quantification help - (reply: 1)
112. cDNA amount in qPCR - (reply: 2)
113. mRNA integrity for qPCR - (reply: 6)
114. Strange control contamination in cDNA synthesis - (reply: 2)
115. how does polymerase stop at the required length - (reply: 1)
116. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 6)
117. Real-time efficiency question - (reply: 1)
118. Selective removal of standards post qPCR Run - (reply: 1)
119. Primer concentration - stupid question - (reply: 3)
120. Primer Design, help i´m New - (reply: 4)
92. skip 65 degrees protocol of Reverse transcription - (reply: 4)
93. Mystery in my PCR - (reply: 5)
94. Whole genome amplification from cDNA? - (reply: 4)
95. What do you use to check primer secondary structure? - (reply: 3)
96. CT of qPCR - (reply: 3)
97. Need help with my real time RT-PCR Plate Set up - (reply: 8)
98. PCR product as template for in vitro transcription - (reply: 1)
99. Sybr Green vs Taqman -- a practical approach - (reply: 2)
100. Problem with 3 step PCR - (reply: 3)
101. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
102. ARMS-PCR - (reply: 1)
103. Scaling up PCR to get more DNA - (reply: 5)
104. RNA concentration suddenly drops - (reply: 3)
105. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
106. PCR to get 10kbp product - (reply: 4)
107. site-directed mutagenesis primer Tm - (reply: 4)
108. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
109. RNA concentration to start RT reaction - (reply: 1)
110. Random vs oligo primer in preamplification RT - (reply: 1)
111. Smart Cycler II software and Absolute quantification help - (reply: 1)
112. cDNA amount in qPCR - (reply: 2)
113. mRNA integrity for qPCR - (reply: 6)
114. Strange control contamination in cDNA synthesis - (reply: 2)
115. how does polymerase stop at the required length - (reply: 1)
116. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 6)
117. Real-time efficiency question - (reply: 1)
118. Selective removal of standards post qPCR Run - (reply: 1)
119. Primer concentration - stupid question - (reply: 3)
120. Primer Design, help i´m New - (reply: 4)