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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
91. website for primer design - (reply: 1)
92. PCR Efficiency over 150%! - (reply: 1)
93. problem of amplification - (reply: 2)
94. Rxn not working - amplify/linearize plasmid - (reply: 3)
95. qRT-PCR works one day, and not the next - (reply: 1)
96. PCR not working - (reply: 11)
97. an urgent problem with replicates - (reply: 10)
98. Site-directed mutagenesis: maximum substitution - (reply: 1)
99. Inter run calibration qRT-PCR - (reply: 2)
100. Primer design and alternative transcripts - (reply: 2)
101. qPCR amplification - (reply: 4)
102. Extremely desperate noob question: How do these PCR work? - (reply: 6)
103. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
104. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
105. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
106. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
107. Genomic DNA Isolation from Soft Agar Colonies - (reply: 3)
108. help with total RNA isolation - (reply: 1)
109. Need help to identify bands in AGE result - (reply: 3)
110. COLD-PCR (Dissociation Curve) via qPCR - (reply: 4)
111. how to hasten real-time PCR amplifications - (reply: 2)
112. Please please help me with my Phusion PCR. - (reply: 5)
113. Nanodrop vs Picogreen to quantify post-PCR product - (reply: 3)
114. Need no RT control for all genes? - (reply: 1)
115. Different annealing temp between HKG and target gene - (reply: 2)
116. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
117. help in long pcr - (reply: 1)
118. Reselling a supermix/enzyme in a commercial diagnostic kit - (reply: 2)
119. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
120. PCR inhibitor in template DNA - (reply: 3)