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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
691. Using BLAST to check primers - (reply: 1)
692. What is the function of a third primer in a PCR mix?? - (reply: 2)
693. How can we design a primer ? step by step method - (reply: 4)
694. Desperate! qPCR standard curve problems - (reply: 2)
695. cDNA synthesis or RT-PCR - (reply: 3)
696. PCR on cDNA and SGamplification??? - (reply: 1)
697. real time PCR protocol - controls? - (reply: 2)
698. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
699. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
700. SOS: new real time PCR machine needed.... which one? - (reply: 5)
701. Amplicon side products - (reply: 5)
702. Fold change graph - (reply: 1)
703. Statistical analyses of 2 - delta delta Ct values - (reply: 2)
704. Finding the right sequence - (reply: 4)
705. Buy BAC vectors - (reply: 1)
706. meaningless real time results - (reply: 1)
707. RT-PCR in a single tube - (reply: 3)
708. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
709. annealing temperature for 3 primer pairs? - (reply: 4)
710. Heated-lid - (reply: 3)
711. DEPC treated water Vs HPLC grade water - (reply: 2)
712. PCR Reaction - (reply: 3)
713. How to interpret this agarose gel? - (reply: 6)
714. low pcr product - (reply: 3)
715. PCR to amplify my insert not working - (reply: 6)
716. HRM analysis, equipment requirements - (reply: 3)
717. AQ vs RQ - (reply: 3)
718. isolation of an already frozen tissue sample - (reply: 1)
719. Problem with PCR amplification- high 3' stability - (reply: 3)
720. Home made real-time PCR problem - (reply: 4)