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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
691. Message Amount in qPCR - (reply: 1)
692. Running Primers on Agarose Gel - (reply: 6)
693. Explanation needed for melting curve - (reply: 3)
694. cDNA concentration of qPCR - (reply: 1)
695. pGEX vector - (reply: 2)
696. will the template of PCR be added A ? - (reply: 2)
697. qPCR normalization of reference gene - (reply: 1)
698. Primer design: Tm above or below Ta? - (reply: 5)
699. Design primers for expression cloning - (reply: 11)
700. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
701. primer dilution query - (reply: 2)
702. Real Time PCR polymerase enzyme - (reply: 3)
703. housekeeping gene has not uniform pattern - (reply: 3)
704. Using BLAST to check primers - (reply: 1)
705. What is the function of a third primer in a PCR mix?? - (reply: 2)
706. How can we design a primer ? step by step method - (reply: 4)
707. Desperate! qPCR standard curve problems - (reply: 2)
708. cDNA synthesis or RT-PCR - (reply: 3)
709. PCR on cDNA and SGamplification??? - (reply: 1)
710. real time PCR protocol - controls? - (reply: 2)
711. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
712. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
713. SOS: new real time PCR machine needed.... which one? - (reply: 5)
714. Amplicon side products - (reply: 5)
715. Fold change graph - (reply: 1)
716. Statistical analyses of 2 - delta delta Ct values - (reply: 2)
717. Finding the right sequence - (reply: 4)
718. Buy BAC vectors - (reply: 1)
719. meaningless real time results - (reply: 1)
720. RT-PCR in a single tube - (reply: 3)