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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
691. Preference for larger of 2 bands? - (reply: 2)
692. Tm of primers - prediction and verification - (reply: 1)
693. acceptable variation for biological replicate - (reply: 2)
694. RT-PCR contamination in negative control. - (reply: 1)
695. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
696. Technical problem with ABI7300 - (reply: 4)
697. cdna synthesis - (reply: 1)
698. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
699. Help with primer design - (reply: 3)
700. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)
701. PCR smearing and...problems! - (reply: 7)
702. Message Amount in qPCR - (reply: 1)
703. Running Primers on Agarose Gel - (reply: 6)
704. Explanation needed for melting curve - (reply: 3)
705. cDNA concentration of qPCR - (reply: 1)
706. pGEX vector - (reply: 2)
707. will the template of PCR be added A ? - (reply: 2)
708. qPCR normalization of reference gene - (reply: 1)
709. Primer design: Tm above or below Ta? - (reply: 5)
710. Design primers for expression cloning - (reply: 11)
711. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
712. primer dilution query - (reply: 2)
713. Real Time PCR polymerase enzyme - (reply: 3)
714. housekeeping gene has not uniform pattern - (reply: 3)
715. Using BLAST to check primers - (reply: 1)
716. What is the function of a third primer in a PCR mix?? - (reply: 2)
717. How can we design a primer ? step by step method - (reply: 4)
718. Desperate! qPCR standard curve problems - (reply: 2)
719. cDNA synthesis or RT-PCR - (reply: 3)
720. PCR on cDNA and SGamplification??? - (reply: 1)