Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
691. Housekeeping gene for COS-1 cells - (reply: 1)
692. PCR, neg control & no. of cycles - (reply: 2)
693. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
694. efficiency calculation from non-10 fold dilutions - (reply: 1)
695. whole plasmid pcr? - (reply: 6)
696. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
697. Genomic pseudogenes?!? - (reply: 6)
698. Strange amplification plot - (reply: 4)
699. Conventional PCR of different dilutions - (reply: 1)
700. primer resuspension - (reply: 2)
701. Principles of overlap PCR - (reply: 7)
702. Help designing primers with restriction sites - (reply: 3)
703. DNA sequence data came back with lots of wrong bases - (reply: 5)
704. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
705. Abnormal amplification curve - (reply: 1)
706. Problem with Semiquantitative PCR - (reply: 4)
707. false positive/cross reaction with TaqMan - (reply: 1)
708. Problem w/ Genotyping Cre Only - (reply: 3)
709. ROX reference dye for quantitative RT-PCR - (reply: 1)
710. PCR problem - (reply: 9)
711. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
712. why PCR product smeared? - (reply: 2)
713. Tm calculation for primers with RE sites and overhangs - (reply: 4)
714. silincing box, no amplification - (reply: 3)
715. Comparative semiquantitative RT-PCR - (reply: 2)
716. Bands in my PCR Controls - (reply: 3)
717. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
718. real time qRT-PCR and low expression gene - (reply: 2)
719. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
720. check points while preparing for sequencing - (reply: 10)