Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
691. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
692. Total RNA estimation - (reply: 2)
693. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
694. pcr contamination - (reply: 4)
695. Large Non-specfic bands in PCR - (reply: 3)
696. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
697. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
698. Primer dilution --> problem.. - (reply: 16)
699. no bands after pcr - even after we have change most of the reagents (reply: 2)
700. High resolution melting to gene scanning - (reply: 1)
701. Help! Smear in Real-Time PCR Product - (reply: 1)
702. Is one reference gene ever appropriate? - (reply: 3)
703. Sybr green RT-qPCR primer - (reply: 1)
704. Sybr green RT-qPCR primer - (reply: 1)
705. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
706. Oligo Question - (reply: 1)
707. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
708. real time rt PCR - bloodyurine - inhibition - (reply: 1)
709. No Amplification in PCR - (reply: 4)
710. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
711. Real time PCR and percentage loss - (reply: 1)
712. Problem with smear in PCR with certain templates - (reply: 4)
713. running multiple qPCR with common housekeeping control - (reply: 3)
714. Problems with PCR in general - (reply: 1)
715. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
716. Control inconsistence in Q-PCR - (reply: 1)
717. how to overcome primer contamination - need protocol (reply: 11)
718. To quantify cDNA or total RNA for real-time gene expression analysis? - (reply: 4)
719. sequencing problem - (reply: 1)
720. High Resolution Melting (HRM) Problem - (reply: 4)