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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
691. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
692. Abnormal amplification curve - (reply: 1)
693. Problem with Semiquantitative PCR - (reply: 4)
694. false positive/cross reaction with TaqMan - (reply: 1)
695. Problem w/ Genotyping Cre Only - (reply: 3)
696. ROX reference dye for quantitative RT-PCR - (reply: 1)
697. PCR problem - (reply: 9)
698. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
699. why PCR product smeared? - (reply: 2)
700. Tm calculation for primers with RE sites and overhangs - (reply: 4)
701. silincing box, no amplification - (reply: 3)
702. Comparative semiquantitative RT-PCR - (reply: 2)
703. Bands in my PCR Controls - (reply: 3)
704. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
705. real time qRT-PCR and low expression gene - (reply: 2)
706. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
707. check points while preparing for sequencing - (reply: 10)
708. Disagreement between triplicate results in Q -PCR - (reply: 5)
709. 40 cT values any idea how to lower it? - (reply: 1)
710. Trizol and Protein extraction - (reply: 2)
711. RT-PCR-CELL NUMBER - (reply: 2)
712. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
713. RNA extraction and preparation - RTqPCR - (reply: 3)
714. Mysterious -NRT control - (reply: 4)
715. PYO pcr stopped working. - (reply: 1)
716. Bands seen with qPCR missing in regular PCR - (reply: 5)
717. PCR product one day, none the next day - (reply: 4)
718. PCR using CDNA as template - (reply: 2)
719. why cant get the band? - (reply: 4)
720. CT value too small - (reply: 1)