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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
691. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)
692. PCR smearing and...problems! - (reply: 7)
693. Message Amount in qPCR - (reply: 1)
694. Running Primers on Agarose Gel - (reply: 6)
695. Explanation needed for melting curve - (reply: 3)
696. cDNA concentration of qPCR - (reply: 1)
697. pGEX vector - (reply: 2)
698. will the template of PCR be added A ? - (reply: 2)
699. qPCR normalization of reference gene - (reply: 1)
700. Primer design: Tm above or below Ta? - (reply: 5)
701. Design primers for expression cloning - (reply: 11)
702. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
703. primer dilution query - (reply: 2)
704. Real Time PCR polymerase enzyme - (reply: 3)
705. housekeeping gene has not uniform pattern - (reply: 3)
706. Using BLAST to check primers - (reply: 1)
707. What is the function of a third primer in a PCR mix?? - (reply: 2)
708. How can we design a primer ? step by step method - (reply: 4)
709. Desperate! qPCR standard curve problems - (reply: 2)
710. cDNA synthesis or RT-PCR - (reply: 3)
711. PCR on cDNA and SGamplification??? - (reply: 1)
712. real time PCR protocol - controls? - (reply: 2)
713. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
714. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
715. SOS: new real time PCR machine needed.... which one? - (reply: 5)
716. Amplicon side products - (reply: 5)
717. Fold change graph - (reply: 1)
718. Statistical analyses of 2 - delta delta Ct values - (reply: 2)
719. Finding the right sequence - (reply: 4)
720. Buy BAC vectors - (reply: 1)