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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
691. Primer design: Tm above or below Ta? - (reply: 5)
692. Design primers for expression cloning - (reply: 11)
693. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
694. primer dilution query - (reply: 2)
695. Real Time PCR polymerase enzyme - (reply: 3)
696. housekeeping gene has not uniform pattern - (reply: 3)
697. Using BLAST to check primers - (reply: 1)
698. What is the function of a third primer in a PCR mix?? - (reply: 2)
699. How can we design a primer ? step by step method - (reply: 4)
700. Desperate! qPCR standard curve problems - (reply: 2)
701. cDNA synthesis or RT-PCR - (reply: 3)
702. PCR on cDNA and SGamplification??? - (reply: 1)
703. real time PCR protocol - controls? - (reply: 2)
704. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
705. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
706. SOS: new real time PCR machine needed.... which one? - (reply: 5)
707. Amplicon side products - (reply: 5)
708. Fold change graph - (reply: 1)
709. Statistical analyses of 2 - delta delta Ct values - (reply: 2)
710. Finding the right sequence - (reply: 4)
711. Buy BAC vectors - (reply: 1)
712. meaningless real time results - (reply: 1)
713. RT-PCR in a single tube - (reply: 3)
714. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
715. annealing temperature for 3 primer pairs? - (reply: 4)
716. Heated-lid - (reply: 3)
717. DEPC treated water Vs HPLC grade water - (reply: 2)
718. PCR Reaction - (reply: 3)
719. How to interpret this agarose gel? - (reply: 6)
720. low pcr product - (reply: 3)