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PCR--RT-PCR-and-Real-Time-PCR
691.
primer dilutions -
(reply: 4)
692.
Genotyping PCR primers -
(reply: 1)
693.
Standard curve using plasmid -
(reply: 1)
694.
How to compare these results? Please comment. -
(reply: 5)
695.
Primers too dilute -
(reply: 1)
696.
Amplification curves jagged -
(reply: 1)
697.
ddCT issue: no endogenous expression in control. -
(reply: 4)
698.
Convering 1:5 serial dilutions to logarithm scale for efficiency curves -
(reply: 3)
699.
No PCR product in site-directed mutagenesis -
(reply: 4)
700.
Giardia/Crypto real time PCR late amplification -
(reply: 1)
701.
How to dilute the template DNA for PCR -
(reply: 7)
702.
Desired length of overlap region for OE-PCR -
(reply: 6)
703.
After I run my pcr on agarose the DNA is still in the well -
(reply: 3)
704.
Need help for PCR -
(reply: 5)
705.
scorpion probe -
(reply: 8)
706.
Too low Ct value for reference gene !!! -
(reply: 3)
707.
unespecific band PCR from mouse genomic DNA -
(reply: 9)
708.
Help with primer design through PRIMER3 -
(reply: 4)
709.
Help with unknown band in restriction digest -
(reply: 1)
710.
how to chose right subsequent for her2(erbb2)gene ? -
(reply: 3)
711.
454 sequencing -
(reply: 2)
712.
Preference for larger of 2 bands? -
(reply: 2)
713.
Tm of primers - prediction and verification -
(reply: 1)
714.
acceptable variation for biological replicate -
(reply: 2)
715.
RT-PCR contamination in negative control. -
(reply: 1)
716.
PCR malaria diagnosis nested PCR smear and non specific bands -
(reply: 3)
717.
Technical problem with ABI7300 -
(reply: 4)
718.
cdna synthesis -
(reply: 1)
719.
how to do RT-PCR for16srRNA of a certain bacteria? -
(reply: 4)
720.
Help with primer design -
(reply: 3)
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