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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1081. inter-run calibration - (reply: 4)
1082. Delta Delta Ct method for multiple reference genes? - (reply: 1)
1083. FAM and VIC labelling - (reply: 2)
1084. Lost cause?? - I made a silly (reply: 4)
1085. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
1086. confusing PCR - (reply: 1)
1087. difference between PCR primers and sequencing primers - (reply: 1)
1088. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
1089. making gel electrophoresis - (reply: 1)
1090. housekeeping genes in PCR - (reply: 1)
1091. Question about RealTime PCR Primer Design - (reply: 1)
1092. Primers stopped working!? - PCR Primers (reply: 6)
1093. NFK beta primers - (reply: 4)
1094. final PCR product - need clarifications (reply: 1)
1095. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
1096. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
1097. Ct value and interpretation - (reply: 3)
1098. 16S rDNA - Bacteria identification (reply: 1)
1099. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
1100. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
1101. gene expressional analysis - (reply: 1)
1102. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
1103. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
1104. why genomic can't be use for pcr? - (reply: 2)
1105. Re-use unamplified PCR product - (reply: 2)
1106. 96 well plate plan that converts into a list automatically - (reply: 2)
1107. Reason for odd PCR conditions - (reply: 3)
1108. pcr - problem in pcr (reply: 2)
1109. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
1110. How to present and analyze these real-time PCR data? - (reply: 2)