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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1081. PCR with one primer - (reply: 1)
1082. cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
1083. First strand synthesis - (reply: 1)
1084. PCR contamination - (reply: 19)
1085. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1086. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1087. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1088. PCR not working - No amplification - (reply: 5)
1089. Quantification of PCR products - (reply: 1)
1090. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)
1091. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1092. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1093. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1094. Nested PCR question - (reply: 1)
1095. High Tm on primers - cannot get a product - (reply: 8)
1096. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1097. Help with Multiplex Nested PCR - (reply: 2)
1098. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1099. trembling dissociation curves in HRM assays - (reply: 3)
1100. Fluorescein storage? - (reply: 2)
1101. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1102. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1103. TELO2 & GAPDH - (reply: 2)
1104. PCR -No band formation - (reply: 1)
1105. 3' race - (reply: 1)
1106. PCR AMPLIFICATION - (reply: 1)
1107. internal control for genomic DNA - (reply: 1)
1108. qPCR Data Analysis - Software (reply: 3)
1109. Gene expression confusion - (reply: 5)
1110. real time pcr need advise - (reply: 2)