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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1081. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
1082. confusing PCR - (reply: 1)
1083. difference between PCR primers and sequencing primers - (reply: 1)
1084. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
1085. making gel electrophoresis - (reply: 1)
1086. housekeeping genes in PCR - (reply: 1)
1087. Question about RealTime PCR Primer Design - (reply: 1)
1088. Primers stopped working!? - PCR Primers (reply: 6)
1089. NFK beta primers - (reply: 4)
1090. final PCR product - need clarifications (reply: 1)
1091. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
1092. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
1093. Ct value and interpretation - (reply: 3)
1094. 16S rDNA - Bacteria identification (reply: 1)
1095. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
1096. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
1097. gene expressional analysis - (reply: 1)
1098. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
1099. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
1100. why genomic can't be use for pcr? - (reply: 2)
1101. Re-use unamplified PCR product - (reply: 2)
1102. 96 well plate plan that converts into a list automatically - (reply: 2)
1103. Reason for odd PCR conditions - (reply: 3)
1104. pcr - problem in pcr (reply: 2)
1105. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
1106. How to present and analyze these real-time PCR data? - (reply: 2)
1107. Deoxyribosenucleoside vs. Deoxyribosenucleotide - What does dNTP stand for? (reply: 2)
1108. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
1109. delta-delta-Ct method - what to do, when no expression in healthy control? (reply: 1)
1110. Trouble with annealing temperature - Trouble with annealing temperature (reply: 3)