Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1081. Multiplex problems - (reply: 6)
1082. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
1083. Q-PCR and RT-PCR - (reply: 3)
1084. inter-run calibration - (reply: 4)
1085. Delta Delta Ct method for multiple reference genes? - (reply: 1)
1086. FAM and VIC labelling - (reply: 2)
1087. Lost cause?? - I made a silly (reply: 4)
1088. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
1089. confusing PCR - (reply: 1)
1090. difference between PCR primers and sequencing primers - (reply: 1)
1091. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
1092. making gel electrophoresis - (reply: 1)
1093. housekeeping genes in PCR - (reply: 1)
1094. Question about RealTime PCR Primer Design - (reply: 1)
1095. Primers stopped working!? - PCR Primers (reply: 6)
1096. NFK beta primers - (reply: 4)
1097. final PCR product - need clarifications (reply: 1)
1098. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
1099. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
1100. Ct value and interpretation - (reply: 3)
1101. 16S rDNA - Bacteria identification (reply: 1)
1102. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
1103. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
1104. gene expressional analysis - (reply: 1)
1105. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
1106. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
1107. why genomic can't be use for pcr? - (reply: 2)
1108. Re-use unamplified PCR product - (reply: 2)
1109. 96 well plate plan that converts into a list automatically - (reply: 2)
1110. Reason for odd PCR conditions - (reply: 3)