Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1081. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
1082. Degenerate primers PCR problem, Please help! - (reply: 2)
1083. degenerated Taqman probes? - (reply: 1)
1084. error message on qPCR mashine - (reply: 1)
1085. Hotstart PCR and unspecific Amplification - (reply: 3)
1086. Primer design help needed - (reply: 1)
1087. Primers no longer work - (reply: 1)
1088. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
1089. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
1090. colony PCR - (reply: 2)
1091. copy number variation - (reply: 3)
1092. How to deal with truncated mRNA or mRNA that has secondary structure - (reply: 2)
1093. Very weird gel - I cannot explain these results (reply: 11)
1094. well position effects (edge and corner) effects in qPCR - using Bio-Rad iCycler iQ (reply: 2)
1095. Cytosolic Housekeeping gene - (reply: 1)
1096. PCR reaction without template gives a product - (reply: 6)
1097. PCR wrong product size - (reply: 6)
1098. PCR very faint product - (reply: 1)
1099. proteinase k inhibition/inactivation - method... (reply: 2)
1100. What does melting temperature indicate and how to predict annealing temperature - (reply: 9)
1101. 4 question for real time PCR - (reply: 5)
1102. RNA quality - (reply: 3)
1103. Superscript III - No second strand synthesis? - (reply: 1)
1104. cDNA convertion to DNA - (reply: 4)
1105. PCR didn't work - suspect the enzyme (reply: 2)
1106. How to check RNA quality - (reply: 5)
1107. Colony PCR Specificity - (reply: 2)
1108. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
1109. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 9)
1110. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)