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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1081. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
1082. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
1083. Ct value and interpretation - (reply: 3)
1084. 16S rDNA - Bacteria identification (reply: 1)
1085. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
1086. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
1087. gene expressional analysis - (reply: 1)
1088. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
1089. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
1090. why genomic can't be use for pcr? - (reply: 2)
1091. Re-use unamplified PCR product - (reply: 2)
1092. 96 well plate plan that converts into a list automatically - (reply: 2)
1093. Reason for odd PCR conditions - (reply: 3)
1094. pcr - problem in pcr (reply: 2)
1095. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
1096. How to present and analyze these real-time PCR data? - (reply: 2)
1097. Deoxyribosenucleoside vs. Deoxyribosenucleotide - What does dNTP stand for? (reply: 2)
1098. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
1099. delta-delta-Ct method - what to do, when no expression in healthy control? (reply: 1)
1100. Trouble with annealing temperature - Trouble with annealing temperature (reply: 3)
1101. PCR Master mix - (reply: 2)
1102. Real Time PCR Primers and Probes - (reply: 2)
1103. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
1104. Dead genotyping PCR - I can't see s*** (reply: 5)
1105. Any one has light cycler 480 system operator's mannual? - Urgent!!!!!!!!!!!! (reply: 3)
1106. PCR product wrong size! Need help! - (reply: 9)
1107. pooling different pcr cycles? - (reply: 1)
1108. Amplification of concatenated linear ligated fragments - (reply: 4)
1109. Clinical Real-time PCR assay - (reply: 1)
1110. Alternative software to read .sds files? - (reply: 2)