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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1081. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
1082. delta-delta-Ct method - what to do, when no expression in healthy control? (reply: 1)
1083. Trouble with annealing temperature - Trouble with annealing temperature (reply: 3)
1084. PCR Master mix - (reply: 2)
1085. Real Time PCR Primers and Probes - (reply: 2)
1086. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
1087. Dead genotyping PCR - I can't see s*** (reply: 5)
1088. Any one has light cycler 480 system operator's mannual? - Urgent!!!!!!!!!!!! (reply: 3)
1089. PCR product wrong size! Need help! - (reply: 9)
1090. pooling different pcr cycles? - (reply: 1)
1091. Amplification of concatenated linear ligated fragments - (reply: 4)
1092. Clinical Real-time PCR assay - (reply: 1)
1093. Alternative software to read .sds files? - (reply: 2)
1094. primer and promoter sequences - (reply: 1)
1095. Standard curve for real-time PCR - (reply: 2)
1096. delta Ct values - (reply: 1)
1097. Primer Tm differences/ PCR - (reply: 1)
1098. Interpretation of Ct values - (reply: 2)
1099. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
1100. Question about RT... - (reply: 1)
1101. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
1102. PCR Primer Dilution from F+R - (reply: 1)
1103. Plasmid standard problem - (reply: 2)
1104. primer reconstitution - primer reconstitution (reply: 4)
1105. Not-Quite-Nested PCR - (reply: 2)
1106. Primer design for insert amplification - Is this correct? (reply: 2)
1107. got smear on the PCR gel !!! - (reply: 4)
1108. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
1109. Troubleshooting reverse transcriptase-PCR - (reply: 3)
1110. "Unzipped" PCR band - (reply: 3)