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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1081. ChIP primer design - (reply: 1)
1082. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
1083. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
1084. weird QPCR curves - (reply: 2)
1085. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
1086. Questions regarding RT-PCR optimization - (reply: 2)
1087. Problems with semi-quatitative PCR - (reply: 1)
1088. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
1089. Long-amplicon QPCR of mtDNA - (reply: 5)
1090. PCR reaction calculation - (reply: 2)
1091. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
1092. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
1093. no increase in fluorescence in my real time PCR - (reply: 3)
1094. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
1095. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
1096. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
1097. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
1098. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
1099. PCR, using gel extracted band as a template - (reply: 4)
1100. Question of nucleic acid extraction - (reply: 1)
1101. Multiplex problems - (reply: 6)
1102. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
1103. Q-PCR and RT-PCR - (reply: 3)
1104. inter-run calibration - (reply: 4)
1105. Delta Delta Ct method for multiple reference genes? - (reply: 1)
1106. FAM and VIC labelling - (reply: 2)
1107. Lost cause?? - I made a silly (reply: 4)
1108. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
1109. confusing PCR - (reply: 1)
1110. difference between PCR primers and sequencing primers - (reply: 1)