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New Forum Archives (2009-)
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PCR--RT-PCR-and-Real-Time-PCR
1081.
Running trays cracking -
(reply: 4)
1082.
Premix vendor dependent disssociation curves? -
(reply: 3)
1083.
Human 18s rRNA - Human 18s rRNA and qRTPCR normalization
(reply: 7)
1084.
Which Plasmid DNA for standard curve? -
(reply: 5)
1085.
standard curve/ PCR efficiency problems... -
(reply: 1)
1086.
Why is the y-axis labeled 'relative gene expression'? -
(reply: 5)
1087.
Reverse transcription question - Volume for RT reaction
(reply: 3)
1088.
Help! PCR that used to work doesn't work now! -
(reply: 2)
1089.
PCR and templates -
(reply: 3)
1090.
DeltaDelta Ct method and Statistics -
(reply: 2)
1091.
always run internal control for different genes on the differnet plates? -
(reply: 8)
1092.
HELP! Genomic DNA, RNA-size bands -
(reply: 2)
1093.
Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable
(reply: 1)
1094.
Easy primer question? - Primers
(reply: 1)
1095.
PCR inconsistency -
(reply: 4)
1096.
help needed: PCR a gene from genomic DNA -
(reply: 2)
1097.
Does centrifugual speed of Rotor Gene-Q have to calibration? -
(reply: 1)
1098.
questions about pcr products after pooling -
(reply: 3)
1099.
stiching/linking/sewing pcr -
(reply: 3)
1100.
Human mtDNA amplification problems -
(reply: 3)
1101.
qRT-PCR Product Size -
(reply: 5)
1102.
primer dimer - proplems
(reply: 1)
1103.
Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!!
(reply: 2)
1104.
DATA ANALYSIS for qPCR - Calculating 2^(-Del Del Ct).
(reply: 2)
1105.
18s control in qPCR -
(reply: 2)
1106.
weird PCR ask for help -
(reply: 12)
1107.
Absolute quantification and normalization - Are reference genes necessary?
(reply: 1)
1108.
What's the best electronic pipette (Precision) -
(reply: 5)
1109.
No PCR product at all -
(reply: 8)
1110.
PCR troubleshoooooting - primer dimer
(reply: 1)
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