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New Forum Archives (2009-): :
1081. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
1083. PCR stopped working - After changing buffer (reply: 5)
1085. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
1087. PCR protocol questions! - (reply: 2)
1089. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
1091. DNA amount calculation for PCR - (reply: 14)
1093. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
1095. weird QPCR curves - (reply: 2)
1097. Questions regarding RT-PCR optimization - (reply: 2)
1099. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
1101. PCR reaction calculation - (reply: 2)
1103. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
1105. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
1107. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
1109. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
1083. PCR stopped working - After changing buffer (reply: 5)
1085. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
1087. PCR protocol questions! - (reply: 2)
1089. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
1091. DNA amount calculation for PCR - (reply: 14)
1093. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
1095. weird QPCR curves - (reply: 2)
1097. Questions regarding RT-PCR optimization - (reply: 2)
1099. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
1101. PCR reaction calculation - (reply: 2)
1103. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
1105. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
1107. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
1109. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)