Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1081. PCR reaction calculation - (reply: 2)
1082. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
1083. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
1084. no increase in fluorescence in my real time PCR - (reply: 3)
1085. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
1086. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
1087. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
1088. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
1089. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
1090. PCR, using gel extracted band as a template - (reply: 4)
1091. Question of nucleic acid extraction - (reply: 1)
1092. Multiplex problems - (reply: 6)
1093. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
1094. Q-PCR and RT-PCR - (reply: 3)
1095. inter-run calibration - (reply: 4)
1096. Delta Delta Ct method for multiple reference genes? - (reply: 1)
1097. FAM and VIC labelling - (reply: 2)
1098. Lost cause?? - I made a silly (reply: 4)
1099. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
1100. confusing PCR - (reply: 1)
1101. difference between PCR primers and sequencing primers - (reply: 1)
1102. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
1103. making gel electrophoresis - (reply: 1)
1104. housekeeping genes in PCR - (reply: 1)
1105. Question about RealTime PCR Primer Design - (reply: 1)
1106. Primers stopped working!? - PCR Primers (reply: 6)
1107. NFK beta primers - (reply: 4)
1108. final PCR product - need clarifications (reply: 1)
1109. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
1110. Can I use Hot Start Taq polimerase for any target? - (reply: 6)