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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1081. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1082. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1083. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1084. PCR not working - No amplification - (reply: 5)
1085. Quantification of PCR products - (reply: 1)
1086. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)
1087. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1088. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1089. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1090. Nested PCR question - (reply: 1)
1091. High Tm on primers - cannot get a product - (reply: 8)
1092. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1093. Help with Multiplex Nested PCR - (reply: 2)
1094. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1095. trembling dissociation curves in HRM assays - (reply: 3)
1096. Fluorescein storage? - (reply: 2)
1097. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1098. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1099. TELO2 & GAPDH - (reply: 2)
1100. PCR -No band formation - (reply: 1)
1101. 3' race - (reply: 1)
1102. PCR AMPLIFICATION - (reply: 1)
1103. internal control for genomic DNA - (reply: 1)
1104. qPCR Data Analysis - Software (reply: 3)
1105. Gene expression confusion - (reply: 5)
1106. real time pcr need advise - (reply: 2)
1107. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1108. ChIP-qPCR shift in melt curve - (reply: 4)
1109. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
1110. use of housekeeping gene in RT PCR chIP - (reply: 3)