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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
661. Too low Ct value for reference gene !!! - (reply: 3)
662. unespecific band PCR from mouse genomic DNA - (reply: 9)
663. Help with primer design through PRIMER3 - (reply: 4)
664. Help with unknown band in restriction digest - (reply: 1)
665. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
666. 454 sequencing - (reply: 2)
667. Preference for larger of 2 bands? - (reply: 2)
668. Tm of primers - prediction and verification - (reply: 1)
669. acceptable variation for biological replicate - (reply: 2)
670. RT-PCR contamination in negative control. - (reply: 1)
671. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
672. Technical problem with ABI7300 - (reply: 4)
673. cdna synthesis - (reply: 1)
674. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
675. Help with primer design - (reply: 3)
676. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)
677. PCR smearing and...problems! - (reply: 7)
678. Message Amount in qPCR - (reply: 1)
679. Running Primers on Agarose Gel - (reply: 6)
680. Explanation needed for melting curve - (reply: 3)
681. cDNA concentration of qPCR - (reply: 1)
682. pGEX vector - (reply: 2)
683. will the template of PCR be added A ? - (reply: 2)
684. qPCR normalization of reference gene - (reply: 1)
685. Primer design: Tm above or below Ta? - (reply: 5)
686. Design primers for expression cloning - (reply: 11)
687. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
688. primer dilution query - (reply: 2)
689. Real Time PCR polymerase enzyme - (reply: 3)
690. housekeeping gene has not uniform pattern - (reply: 3)