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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
661. RNA extraction from a 1mm brain tissue punch - uncertain about homogenisation - (reply: 2)
662. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
663. PCR is amplifying non-specific fragment - (reply: 6)
664. Anybody know about temperature sensitivity of the AB TaqMan universal Mastermix - (reply: 4)
665. Measuring of cDNA bands density - (reply: 3)
666. real time RT-PCR problems - (reply: 2)
667. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
668. Real-Time efficiencies and the pfaffl method - (reply: 1)
669. should I design primers spanning both intron and exon - (reply: 2)
670. RT-PCR - (reply: 1)
671. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
672. PCR with a very long and a short primer - (reply: 5)
673. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
674. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
675. Dilution of 18s. How much? - (reply: 1)
676. Multiplex PCR does not work - (reply: 3)
677. Alternative to Sybr-green? - (reply: 12)
678. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)
679. ct value of neg - (reply: 1)
680. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
681. Standard curve - (reply: 3)
682. Discuss about COLD-PCR - (reply: 3)
683. Comparison between two primers pairs? Is it possible? - (reply: 3)
684. Rt-PCR primer design - (reply: 7)
685. Length of non-binding sequence in primers with RE site - (reply: 6)
686. qPCR Calc Help - (reply: 6)
687. ABI Prism 7700 software - (reply: 1)
688. Difficult sequences in PCR - (reply: 2)
689. qPCR result interpretation - when your 'relative' is 0?? - (reply: 2)
690. why is real time pcr curve like this? - (reply: 3)