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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
661. Second round of PCR after gel extraction fails miserably - (reply: 3)
662. urgent help plz with RT-PCR - (reply: 3)
663. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
664. LightCycler 480 Melting Curve Assay - (reply: 1)
665. Problems with two step RT-PCR - (reply: 2)
666. Spliced and unspliced form of a gene - (reply: 3)
667. primer dilutions - (reply: 4)
668. Genotyping PCR primers - (reply: 1)
669. Standard curve using plasmid - (reply: 1)
670. How to compare these results? Please comment. - (reply: 5)
671. Primers too dilute - (reply: 1)
672. Amplification curves jagged - (reply: 1)
673. ddCT issue: no endogenous expression in control. - (reply: 4)
674. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
675. No PCR product in site-directed mutagenesis - (reply: 4)
676. Giardia/Crypto real time PCR late amplification - (reply: 1)
677. How to dilute the template DNA for PCR - (reply: 7)
678. Desired length of overlap region for OE-PCR - (reply: 6)
679. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
680. Need help for PCR - (reply: 5)
681. scorpion probe - (reply: 8)
682. Too low Ct value for reference gene !!! - (reply: 3)
683. unespecific band PCR from mouse genomic DNA - (reply: 9)
684. Help with primer design through PRIMER3 - (reply: 4)
685. Help with unknown band in restriction digest - (reply: 1)
686. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
687. 454 sequencing - (reply: 2)
688. Preference for larger of 2 bands? - (reply: 2)
689. Tm of primers - prediction and verification - (reply: 1)
690. acceptable variation for biological replicate - (reply: 2)