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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
661. RT-PCR contamination in negative control. - (reply: 1)
662. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
663. Technical problem with ABI7300 - (reply: 4)
664. cdna synthesis - (reply: 1)
665. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
666. Help with primer design - (reply: 3)
667. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)
668. PCR smearing and...problems! - (reply: 7)
669. Message Amount in qPCR - (reply: 1)
670. Running Primers on Agarose Gel - (reply: 6)
671. Explanation needed for melting curve - (reply: 3)
672. cDNA concentration of qPCR - (reply: 1)
673. pGEX vector - (reply: 2)
674. will the template of PCR be added A ? - (reply: 2)
675. qPCR normalization of reference gene - (reply: 1)
676. Primer design: Tm above or below Ta? - (reply: 5)
677. Design primers for expression cloning - (reply: 11)
678. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
679. primer dilution query - (reply: 2)
680. Real Time PCR polymerase enzyme - (reply: 3)
681. housekeeping gene has not uniform pattern - (reply: 3)
682. Using BLAST to check primers - (reply: 1)
683. What is the function of a third primer in a PCR mix?? - (reply: 2)
684. How can we design a primer ? step by step method - (reply: 4)
685. Desperate! qPCR standard curve problems - (reply: 2)
686. cDNA synthesis or RT-PCR - (reply: 3)
687. PCR on cDNA and SGamplification??? - (reply: 1)
688. real time PCR protocol - controls? - (reply: 2)
689. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
690. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)