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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
661. What temp the lid of the thermocycler must be set? - (reply: 1)
662. How should I optimize my PCR - (reply: 2)
663. Real time data analysis using 2 machines - (reply: 3)
664. Second round of PCR after gel extraction fails miserably - (reply: 3)
665. urgent help plz with RT-PCR - (reply: 3)
666. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
667. LightCycler 480 Melting Curve Assay - (reply: 1)
668. Problems with two step RT-PCR - (reply: 2)
669. Spliced and unspliced form of a gene - (reply: 3)
670. primer dilutions - (reply: 4)
671. Genotyping PCR primers - (reply: 1)
672. Standard curve using plasmid - (reply: 1)
673. How to compare these results? Please comment. - (reply: 5)
674. Primers too dilute - (reply: 1)
675. Amplification curves jagged - (reply: 1)
676. ddCT issue: no endogenous expression in control. - (reply: 4)
677. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
678. No PCR product in site-directed mutagenesis - (reply: 4)
679. Giardia/Crypto real time PCR late amplification - (reply: 1)
680. How to dilute the template DNA for PCR - (reply: 7)
681. Desired length of overlap region for OE-PCR - (reply: 6)
682. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
683. Need help for PCR - (reply: 5)
684. scorpion probe - (reply: 8)
685. Too low Ct value for reference gene !!! - (reply: 3)
686. unespecific band PCR from mouse genomic DNA - (reply: 9)
687. Help with primer design through PRIMER3 - (reply: 4)
688. Help with unknown band in restriction digest - (reply: 1)
689. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
690. 454 sequencing - (reply: 2)