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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
661. primer dilutions - (reply: 4)
662. Genotyping PCR primers - (reply: 1)
663. Standard curve using plasmid - (reply: 1)
664. How to compare these results? Please comment. - (reply: 5)
665. Primers too dilute - (reply: 1)
666. Amplification curves jagged - (reply: 1)
667. ddCT issue: no endogenous expression in control. - (reply: 4)
668. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
669. No PCR product in site-directed mutagenesis - (reply: 4)
670. Giardia/Crypto real time PCR late amplification - (reply: 1)
671. How to dilute the template DNA for PCR - (reply: 7)
672. Desired length of overlap region for OE-PCR - (reply: 6)
673. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
674. Need help for PCR - (reply: 5)
675. scorpion probe - (reply: 8)
676. Too low Ct value for reference gene !!! - (reply: 3)
677. unespecific band PCR from mouse genomic DNA - (reply: 9)
678. Help with primer design through PRIMER3 - (reply: 4)
679. Help with unknown band in restriction digest - (reply: 1)
680. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
681. 454 sequencing - (reply: 2)
682. Preference for larger of 2 bands? - (reply: 2)
683. Tm of primers - prediction and verification - (reply: 1)
684. acceptable variation for biological replicate - (reply: 2)
685. RT-PCR contamination in negative control. - (reply: 1)
686. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
687. Technical problem with ABI7300 - (reply: 4)
688. cdna synthesis - (reply: 1)
689. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
690. Help with primer design - (reply: 3)