Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
661. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)
662. PCR smearing and...problems! - (reply: 7)
663. Message Amount in qPCR - (reply: 1)
664. Running Primers on Agarose Gel - (reply: 6)
665. Explanation needed for melting curve - (reply: 3)
666. cDNA concentration of qPCR - (reply: 1)
667. pGEX vector - (reply: 2)
668. will the template of PCR be added A ? - (reply: 2)
669. qPCR normalization of reference gene - (reply: 1)
670. Primer design: Tm above or below Ta? - (reply: 5)
671. Design primers for expression cloning - (reply: 11)
672. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
673. primer dilution query - (reply: 2)
674. Real Time PCR polymerase enzyme - (reply: 3)
675. housekeeping gene has not uniform pattern - (reply: 3)
676. Using BLAST to check primers - (reply: 1)
677. What is the function of a third primer in a PCR mix?? - (reply: 2)
678. How can we design a primer ? step by step method - (reply: 4)
679. Desperate! qPCR standard curve problems - (reply: 2)
680. cDNA synthesis or RT-PCR - (reply: 3)
681. PCR on cDNA and SGamplification??? - (reply: 1)
682. real time PCR protocol - controls? - (reply: 2)
683. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
684. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
685. SOS: new real time PCR machine needed.... which one? - (reply: 5)
686. Amplicon side products - (reply: 5)
687. Fold change graph - (reply: 1)
688. Statistical analyses of 2 - delta delta Ct values - (reply: 2)
689. Finding the right sequence - (reply: 4)
690. Buy BAC vectors - (reply: 1)