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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
661. pGEX vector - (reply: 2)
662. will the template of PCR be added A ? - (reply: 2)
663. qPCR normalization of reference gene - (reply: 1)
664. Primer design: Tm above or below Ta? - (reply: 5)
665. Design primers for expression cloning - (reply: 11)
666. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
667. primer dilution query - (reply: 2)
668. Real Time PCR polymerase enzyme - (reply: 3)
669. housekeeping gene has not uniform pattern - (reply: 3)
670. Using BLAST to check primers - (reply: 1)
671. What is the function of a third primer in a PCR mix?? - (reply: 2)
672. How can we design a primer ? step by step method - (reply: 4)
673. Desperate! qPCR standard curve problems - (reply: 2)
674. cDNA synthesis or RT-PCR - (reply: 3)
675. PCR on cDNA and SGamplification??? - (reply: 1)
676. real time PCR protocol - controls? - (reply: 2)
677. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
678. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
679. SOS: new real time PCR machine needed.... which one? - (reply: 5)
680. Amplicon side products - (reply: 5)
681. Fold change graph - (reply: 1)
682. Statistical analyses of 2 - delta delta Ct values - (reply: 2)
683. Finding the right sequence - (reply: 4)
684. Buy BAC vectors - (reply: 1)
685. meaningless real time results - (reply: 1)
686. RT-PCR in a single tube - (reply: 3)
687. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
688. annealing temperature for 3 primer pairs? - (reply: 4)
689. Heated-lid - (reply: 3)
690. DEPC treated water Vs HPLC grade water - (reply: 2)