Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
661. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
662. Technical problem with ABI7300 - (reply: 4)
663. cdna synthesis - (reply: 1)
664. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
665. Help with primer design - (reply: 3)
666. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)
667. PCR smearing and...problems! - (reply: 7)
668. Message Amount in qPCR - (reply: 1)
669. Running Primers on Agarose Gel - (reply: 6)
670. Explanation needed for melting curve - (reply: 3)
671. cDNA concentration of qPCR - (reply: 1)
672. pGEX vector - (reply: 2)
673. will the template of PCR be added A ? - (reply: 2)
674. qPCR normalization of reference gene - (reply: 1)
675. Primer design: Tm above or below Ta? - (reply: 5)
676. Design primers for expression cloning - (reply: 11)
677. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
678. primer dilution query - (reply: 2)
679. Real Time PCR polymerase enzyme - (reply: 3)
680. housekeeping gene has not uniform pattern - (reply: 3)
681. Using BLAST to check primers - (reply: 1)
682. What is the function of a third primer in a PCR mix?? - (reply: 2)
683. How can we design a primer ? step by step method - (reply: 4)
684. Desperate! qPCR standard curve problems - (reply: 2)
685. cDNA synthesis or RT-PCR - (reply: 3)
686. PCR on cDNA and SGamplification??? - (reply: 1)
687. real time PCR protocol - controls? - (reply: 2)
688. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
689. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
690. SOS: new real time PCR machine needed.... which one? - (reply: 5)