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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
661. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
662. Help with primer design - (reply: 3)
663. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)
664. PCR smearing and...problems! - (reply: 7)
665. Message Amount in qPCR - (reply: 1)
666. Running Primers on Agarose Gel - (reply: 6)
667. Explanation needed for melting curve - (reply: 3)
668. cDNA concentration of qPCR - (reply: 1)
669. pGEX vector - (reply: 2)
670. will the template of PCR be added A ? - (reply: 2)
671. qPCR normalization of reference gene - (reply: 1)
672. Primer design: Tm above or below Ta? - (reply: 5)
673. Design primers for expression cloning - (reply: 11)
674. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
675. primer dilution query - (reply: 2)
676. Real Time PCR polymerase enzyme - (reply: 3)
677. housekeeping gene has not uniform pattern - (reply: 3)
678. Using BLAST to check primers - (reply: 1)
679. What is the function of a third primer in a PCR mix?? - (reply: 2)
680. How can we design a primer ? step by step method - (reply: 4)
681. Desperate! qPCR standard curve problems - (reply: 2)
682. cDNA synthesis or RT-PCR - (reply: 3)
683. PCR on cDNA and SGamplification??? - (reply: 1)
684. real time PCR protocol - controls? - (reply: 2)
685. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
686. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
687. SOS: new real time PCR machine needed.... which one? - (reply: 5)
688. Amplicon side products - (reply: 5)
689. Fold change graph - (reply: 1)
690. Statistical analyses of 2 - delta delta Ct values - (reply: 2)