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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1891. Need an UV260 RT-PCR instrument - (reply: 1)
1892. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1893. PCR produces products of wrong size - (reply: 4)
1894. PCR with pfu and degenerate primers (?) - (reply: 7)
1895. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1896. Serious issues with RT-PCR - (reply: 7)
1897. relative quantitation problem plz help - (reply: 4)
1898. No beta actin results at all??? - (reply: 1)
1899. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
1900. dNTP question - (reply: 3)
1901. Taqman rtPCR primer and probe design - (reply: 3)
1902. Help with Real Time PCR Well To Well Variation - (reply: 10)
1903. dna for real time - (reply: 3)
1904. Mycobacterial housekeeping genes - (reply: 1)
1905. Expression comparision using Cp values - (reply: 1)
1906. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
1907. Which to use for standard curve? - (reply: 2)
1908. How do you calibrate between runs? - (reply: 3)
1909. Houskeeping Gene not steady - (reply: 7)
1910. real time PCR - (reply: 3)
1911. Real-Time RT-PCR one-step and two step issue - (reply: 13)
1912. Plateau Phase - Taqman not reaching plateau (reply: 1)
1913. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
1914. maximum amplicon size - 550bp too long??? (reply: 5)
1915. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
1916. SYBR Green miRNA analysis - (reply: 1)
1917. What went wrong in my qPCR? - please look at the dissociation curve (reply: 27)
1918. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
1919. Can DEPC-water interfere QPCR reaction? - (reply: 4)
1920. Melting Curve Analysis Difficulties - (reply: 7)