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Top : New Forum Archives (2009-): : PCR, RT-PCR and Real-Time PCR

1891. Mycobacterial housekeeping genes - (reply: 1)
1892. Expression comparision using Cp values - (reply: 1)
1893. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
1894. Which to use for standard curve? - (reply: 2)
1895. How do you calibrate between runs? - (reply: 3)
1896. Houskeeping Gene not steady - (reply: 7)
1897. real time PCR - (reply: 3)
1898. Real-Time RT-PCR one-step and two step issue - (reply: 13)
1899. Plateau Phase - Taqman not reaching plateau (reply: 1)
1900. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
1901. maximum amplicon size - 550bp too long??? (reply: 5)
1902. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
1903. SYBR Green miRNA analysis - (reply: 1)
1904. What went wrong in my qPCR? - please look at the dissociation curve (reply: 27)
1905. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
1906. Can DEPC-water interfere QPCR reaction? - (reply: 4)
1907. Melting Curve Analysis Difficulties - (reply: 7)
1908. SYBR and TaqMan - (reply: 6)
1909. real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
1910. New Technology in Real Time PCR - latest technologies (reply: 2)
1911. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
1912. Important literature for real time PCR users - Important documents for beginners as well as advanced users (reply: 41)
1913. PCR efficiencies - (reply: 4)
1914. Optimizing multiplex RT-qPCR - (reply: 3)
1915. difference between hotstart and taq polymerase - (reply: 7)