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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1501. make your own gotaq - (reply: 2)
1502. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1503. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1504. primer RE over-hang nucleotides - common sequences? (reply: 5)
1505. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1506. Does RT step smplify RNA - (reply: 2)
1507. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1508. ROX utilization - (who uses ROX?) (reply: 1)
1509. qPCR analysis - (reply: 6)
1510. good housekeeping for ovary - (reply: 2)
1511. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
1512. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
1513. primer design problem - (reply: 1)
1514. The perfect RT??? which control? - (reply: 4)
1515. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
1516. qPCR - qPCR problems.. (reply: 5)
1517. is it necessary to do DNases treatment? - (reply: 2)
1518. problem with site directed mutagenesis - (reply: 4)
1519. allele specific pcr - determining genotypes (reply: 2)
1520. Low 260/230 ratio in my RNA - (reply: 3)
1521. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
1522. Problem with primer efficiency analysis - (reply: 4)
1523. calibrator - what is this in qPCR (reply: 1)
1524. slan real time PCR system for validation of microarray results? - (reply: 1)
1525. PCR unusualband at ~230bps - PCR (reply: 1)
1526. DMSO or BSA for PCR - (reply: 6)
1527. primer design - (reply: 1)
1528. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
1529. Help me answer this curious student - Many questions on techniques (reply: 1)
1530. Tris Buffer for PCR reaction - why? (reply: 1)