Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1501. Problem cDNA synthesis/ 2 step RT - (reply: 6)
1502. High MW PCR band seen...help needed - (reply: 7)
1503. QPCR - Primer and Probe question - QPCR (reply: 2)
1504. Urgent Help needed: RNA-Interference, rt-pcr and Western-Blot do not match - (reply: 2)
1505. Running trays cracking - (reply: 4)
1506. Premix vendor dependent disssociation curves? - (reply: 3)
1507. Human 18s rRNA - Human 18s rRNA and qRTPCR normalization (reply: 7)
1508. Which Plasmid DNA for standard curve? - (reply: 5)
1509. standard curve/ PCR efficiency problems... - (reply: 1)
1510. Why is the y-axis labeled 'relative gene expression'? - (reply: 5)
1511. Reverse transcription question - Volume for RT reaction (reply: 3)
1512. Help! PCR that used to work doesn't work now! - (reply: 2)
1513. PCR and templates - (reply: 3)
1514. DeltaDelta Ct method and Statistics - (reply: 2)
1515. always run internal control for different genes on the differnet plates? - (reply: 8)
1516. HELP! Genomic DNA, RNA-size bands - (reply: 2)
1517. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
1518. Easy primer question? - Primers (reply: 1)
1519. PCR inconsistency - (reply: 4)
1520. help needed: PCR a gene from genomic DNA - (reply: 2)
1521. Does centrifugual speed of Rotor Gene-Q have to calibration? - (reply: 1)
1522. questions about pcr products after pooling - (reply: 3)
1523. stiching/linking/sewing pcr - (reply: 3)
1524. Human mtDNA amplification problems - (reply: 3)
1525. qRT-PCR Product Size - (reply: 5)
1526. primer dimer - proplems (reply: 1)
1527. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
1528. DATA ANALYSIS for qPCR - Calculating 2^(-Del Del Ct). (reply: 2)
1529. 18s control in qPCR - (reply: 2)
1530. weird PCR ask for help - (reply: 12)