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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1501. Human mtDNA amplification problems - (reply: 3)
1502. qRT-PCR Product Size - (reply: 5)
1503. primer dimer - proplems (reply: 1)
1504. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
1505. DATA ANALYSIS for qPCR - Calculating 2^(-Del Del Ct). (reply: 2)
1506. 18s control in qPCR - (reply: 2)
1507. weird PCR ask for help - (reply: 12)
1508. Absolute quantification and normalization - Are reference genes necessary? (reply: 1)
1509. What's the best electronic pipette (Precision) - (reply: 5)
1510. No PCR product at all - (reply: 8)
1511. PCR troubleshoooooting - primer dimer (reply: 1)
1512. Primer efficiency test - (reply: 1)
1513. primer software - (reply: 2)
1514. Question on wired PCR - (reply: 1)
1515. Validation of housekeeping gene expression - (reply: 3)
1516. restriction digestion of PCR product - (reply: 7)
1517. reuse pipette tips - (reply: 2)
1518. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
1519. How to know in which exon a primer match? - (reply: 1)
1520. dUTPs in qRTPCR rxn - (reply: 1)
1521. Amplification of human genomic DNA - (reply: 2)
1522. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1523. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1524. make your own gotaq - (reply: 2)
1525. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1526. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1527. primer RE over-hang nucleotides - common sequences? (reply: 5)
1528. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1529. Does RT step smplify RNA - (reply: 2)
1530. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)