Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1501. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
1502. rna storage at -20 - (reply: 2)
1503. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
1504. high Cp value PCR - (reply: 2)
1505. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
1506. Synthesize own Oligo-dT primers - (reply: 6)
1507. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
1508. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
1509. dNTP or Deoxynucleotide solution Mix - (reply: 1)
1510. Smearing/ no amplification in PCR - (reply: 3)
1511. qRTPCR (TaqMan) Data Representation - (reply: 1)
1512. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
1513. Lucicferase activity and endogenous transcription - (reply: 7)
1514. need help on primer calcuation soon - (reply: 1)
1515. Heart ache in real time - (reply: 1)
1516. HEX vs. Cy-5 - (reply: 3)
1517. Direct PCR (PCR without DNA extraction) - (reply: 2)
1518. PCR using xt5 - (reply: 2)
1519. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
1520. Problems with qRT-PCR - Lots of unspecific products (reply: 2)
1521. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
1522. 2 bands or smear? 2-step RT-PCR - (reply: 1)
1523. 23S rRNA Primer - need primer sequence (reply: 1)
1524. Lowered extension temps with Phusion - (reply: 7)
1525. RT-PCR - problems with TaqMan PCR (reply: 2)
1526. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
1527. Two annealing temps? - (reply: 4)
1528. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
1529. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
1530. Small product amplification problems - (reply: 4)