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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1501. How to know in which exon a primer match? - (reply: 1)
1502. dUTPs in qRTPCR rxn - (reply: 1)
1503. Amplification of human genomic DNA - (reply: 2)
1504. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1505. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1506. make your own gotaq - (reply: 2)
1507. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1508. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1509. primer RE over-hang nucleotides - common sequences? (reply: 5)
1510. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1511. Does RT step smplify RNA - (reply: 2)
1512. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1513. ROX utilization - (who uses ROX?) (reply: 1)
1514. qPCR analysis - (reply: 6)
1515. good housekeeping for ovary - (reply: 2)
1516. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
1517. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
1518. primer design problem - (reply: 1)
1519. The perfect RT??? which control? - (reply: 4)
1520. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
1521. qPCR - qPCR problems.. (reply: 5)
1522. is it necessary to do DNases treatment? - (reply: 2)
1523. problem with site directed mutagenesis - (reply: 4)
1524. allele specific pcr - determining genotypes (reply: 2)
1525. Low 260/230 ratio in my RNA - (reply: 3)
1526. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
1527. Problem with primer efficiency analysis - (reply: 4)
1528. calibrator - what is this in qPCR (reply: 1)
1529. slan real time PCR system for validation of microarray results? - (reply: 1)
1530. PCR unusualband at ~230bps - PCR (reply: 1)