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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1501. 23S rRNA Primer - need primer sequence (reply: 1)
1502. Lowered extension temps with Phusion - (reply: 7)
1503. RT-PCR - problems with TaqMan PCR (reply: 2)
1504. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
1505. Two annealing temps? - (reply: 4)
1506. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
1507. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
1508. Small product amplification problems - (reply: 4)
1509. ROX and Rotorgene - using a mix with reference dye in Rotorgene (reply: 2)
1510. Freezing primers+SYBR green - (reply: 2)
1511. Tagged Primers - (reply: 5)
1512. no pcr amplification product - (reply: 9)
1513. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
1514. less steep curve and high Ct value! - (reply: 2)
1515. Question about site directed mutagenesis - (reply: 1)
1516. Amplfication of fungal DNA - (reply: 8)
1517. PCR problem - (reply: 4)
1518. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
1519. differently behavioring replicates in real-time PCR - (reply: 1)
1520. Multiplex PCR - (reply: 1)
1521. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1522. optimal template dna concentrations - (reply: 1)
1523. reconstitution of primers - (reply: 2)
1524. Mysterious PCR Contamination - (reply: 13)
1525. RT-PCR Help - Protocol provided (reply: 4)
1526. Endogenous control in bacterial RT-qPCR - (reply: 4)
1527. Mutagenesis PCR and undesired amplification - (reply: 2)
1528. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1529. primer dimer - (reply: 2)
1530. Genotyping conditioning-where to start? - (reply: 1)