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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1501. PCR troubleshoooooting - primer dimer (reply: 1)
1502. Primer efficiency test - (reply: 1)
1503. primer software - (reply: 2)
1504. Question on wired PCR - (reply: 1)
1505. Validation of housekeeping gene expression - (reply: 3)
1506. restriction digestion of PCR product - (reply: 7)
1507. reuse pipette tips - (reply: 2)
1508. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
1509. How to know in which exon a primer match? - (reply: 1)
1510. dUTPs in qRTPCR rxn - (reply: 1)
1511. Amplification of human genomic DNA - (reply: 2)
1512. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1513. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1514. make your own gotaq - (reply: 2)
1515. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1516. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1517. primer RE over-hang nucleotides - common sequences? (reply: 5)
1518. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1519. Does RT step smplify RNA - (reply: 2)
1520. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1521. ROX utilization - (who uses ROX?) (reply: 1)
1522. qPCR analysis - (reply: 6)
1523. good housekeeping for ovary - (reply: 2)
1524. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
1525. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
1526. primer design problem - (reply: 1)
1527. The perfect RT??? which control? - (reply: 4)
1528. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
1529. qPCR - qPCR problems.. (reply: 5)
1530. is it necessary to do DNases treatment? - (reply: 2)