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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1501. Question on wired PCR - (reply: 1)
1502. Validation of housekeeping gene expression - (reply: 3)
1503. restriction digestion of PCR product - (reply: 7)
1504. reuse pipette tips - (reply: 2)
1505. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
1506. How to know in which exon a primer match? - (reply: 1)
1507. dUTPs in qRTPCR rxn - (reply: 1)
1508. Amplification of human genomic DNA - (reply: 2)
1509. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1510. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1511. make your own gotaq - (reply: 2)
1512. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1513. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1514. primer RE over-hang nucleotides - common sequences? (reply: 5)
1515. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1516. Does RT step smplify RNA - (reply: 2)
1517. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1518. ROX utilization - (who uses ROX?) (reply: 1)
1519. qPCR analysis - (reply: 6)
1520. good housekeeping for ovary - (reply: 2)
1521. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
1522. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
1523. primer design problem - (reply: 1)
1524. The perfect RT??? which control? - (reply: 4)
1525. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
1526. qPCR - qPCR problems.. (reply: 5)
1527. is it necessary to do DNases treatment? - (reply: 2)
1528. problem with site directed mutagenesis - (reply: 4)
1529. allele specific pcr - determining genotypes (reply: 2)
1530. Low 260/230 ratio in my RNA - (reply: 3)