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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1501. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1502. asymmetric PCR - (reply: 1)
1503. Best time to isolate RNA for qPCR? - (reply: 1)
1504. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
1505. CORRECT PCR Incorrect RTPCR - (reply: 10)
1506. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1507. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1508. Real time PCR results-interpretation - (reply: 2)
1509. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1510. Bands appearing out of no where?! - (reply: 5)
1511. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1512. SYBR melting curve vs RT-PCR gel - (reply: 1)
1513. searching housekeeping genes - (reply: 4)
1514. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1515. primer tm - tm calculation for tagged primers (reply: 2)
1516. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1517. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1518. Long real-time Amplicon - (reply: 2)
1519. PCR + phusion enzyme = massive errors - (reply: 4)
1520. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1521. RNA from MMA embedded calcified bone - (reply: 1)
1522. long DNA amplification - (reply: 11)
1523. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1524. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1525. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1526. Amplify cDNA for qRT-PCR? - (reply: 6)
1527. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1528. QPCR problem! - (reply: 9)
1529. pcr trouble solved but I cant seem to understand why - (reply: 1)
1530. Primer design if sequence is unknown for organism - (reply: 5)