Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1501. What Factors Affect Ct values of a gene? - (reply: 1)
1502. Sybr Green I in real-time PCR reaction - (reply: 3)
1503. Primers annealing temperatures - (reply: 8)
1504. Difficulties cloning by PCR - (reply: 5)
1505. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
1506. rna storage at -20 - (reply: 2)
1507. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
1508. high Cp value PCR - (reply: 2)
1509. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
1510. Synthesize own Oligo-dT primers - (reply: 6)
1511. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
1512. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
1513. dNTP or Deoxynucleotide solution Mix - (reply: 1)
1514. Smearing/ no amplification in PCR - (reply: 3)
1515. qRTPCR (TaqMan) Data Representation - (reply: 1)
1516. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
1517. Lucicferase activity and endogenous transcription - (reply: 7)
1518. need help on primer calcuation soon - (reply: 1)
1519. Heart ache in real time - (reply: 1)
1520. HEX vs. Cy-5 - (reply: 3)
1521. Direct PCR (PCR without DNA extraction) - (reply: 2)
1522. PCR using xt5 - (reply: 2)
1523. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
1524. Problems with qRT-PCR - Lots of unspecific products (reply: 2)
1525. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
1526. 2 bands or smear? 2-step RT-PCR - (reply: 1)
1527. 23S rRNA Primer - need primer sequence (reply: 1)
1528. Lowered extension temps with Phusion - (reply: 7)
1529. RT-PCR - problems with TaqMan PCR (reply: 2)
1530. qRT-PCR calculations - Averaging across biological replicates (reply: 2)