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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
541. How to measure expression the mRNA (quantitative) - (reply: 1)
542. Ethanol contamination in RNA - (reply: 11)
543. Normalization of Real time PCR results using Pfaffl method - (reply: 2)
544. RT-PCR vs plate reader for pathogen detection - (reply: 3)
545. How to check primer sequences using BLAST and other tools? - (reply: 2)
546. real time PCR analysis in patient samples - (reply: 2)
547. Experiment plan for RT-qPCR internal reference search - (reply: 3)
548. Primer Check? - (reply: 2)
549. amplification of 14Kb - (reply: 1)
550. Validation of PCR primers/ probes - (reply: 6)
551. genomic DNA extraction from formalin fixed tissues (not embedded) for genotyping - (reply: 4)
552. Real time PCR sudenly not working - (reply: 1)
553. Buffer-composition One-Step RT-PCR - (reply: 4)
554. Two reverse primer sequences for a single forward primer - (reply: 1)
555. Tm variation - (reply: 1)
556. PCR with more than one primer - (reply: 1)
557. RAPD - PCR problem - (reply: 5)
558. Relative fold expression and Normalization fold expression - (reply: 2)
559. Strange Bands - (reply: 2)
560. Recommended tamplate DNA concentration for qPCR - (reply: 2)
561. RT / cDNA synthesis - (reply: 7)
562. How to best store PCR product? - (reply: 2)
563. smearing below band of interest - (reply: 5)
564. RT-PCR primer design - (reply: 1)
565. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
566. Odd gel run following PCR - (reply: 28)
567. Qiagen PCR Array Reagents? - (reply: 1)
568. Enough checks for gDNA contamination in qPCR? - (reply: 3)
569. plate stuck in ABI7300 - (reply: 7)
570. No or very weak band using 16s primers - (reply: 1)