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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
541. QuikChange with Different plasmids - (reply: 1)
542. Good melting curve but non-specific products appeared - (reply: 2)
543. Long Non-Specific PCR Products - (reply: 5)
544. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
545. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
546. fold change (how to represent it) - (reply: 1)
547. PCR - consistent false positive results - (reply: 6)
548. geNorm alternative for qPCR housekeeping genes - (reply: 1)
549. PCR no amplification - (reply: 15)
550. positive NTC in real time reaction - (reply: 12)
551. Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
552. How to quantify virus by PCR - (reply: 12)
553. qPCR contamination problem - (reply: 5)
554. Error rate of commecial reverse transcriptase - (reply: 1)
555. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
556. low A260/230 problem - (reply: 2)
557. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
558. Pcr product size determination for a fusion gene - (reply: 8)
559. open ABI 7500 documents in Macbook - (reply: 1)
560. Primer Annealing Temperatures - (reply: 24)
561. Molecular beacons problem - (reply: 6)
562. RT-qPCR plate set up NoRT controls - (reply: 1)
563. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
564. same primers, different product in conventional PCR and qPCR - (reply: 6)
565. NEB Q5 polymerase works very well - (reply: 1)
566. Plateau height qPCR - (reply: 3)
567. UDG in qPCR - (reply: 1)
568. 5'RACE - (reply: 3)
569. Taqman Probes problem - (reply: 3)
570. qPCR : why substracting CT values instead of dividing ? - (reply: 3)