Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
541. PCR smearing and...problems! - (reply: 7)
542. Message Amount in qPCR - (reply: 1)
543. Running Primers on Agarose Gel - (reply: 6)
544. Explanation needed for melting curve - (reply: 3)
545. cDNA concentration of qPCR - (reply: 1)
546. pGEX vector - (reply: 2)
547. will the template of PCR be added A ? - (reply: 2)
548. qPCR normalization of reference gene - (reply: 1)
549. Primer design: Tm above or below Ta? - (reply: 5)
550. Design primers for expression cloning - (reply: 11)
551. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
552. primer dilution query - (reply: 2)
553. Real Time PCR polymerase enzyme - (reply: 3)
554. housekeeping gene has not uniform pattern - (reply: 3)
555. Using BLAST to check primers - (reply: 1)
556. What is the function of a third primer in a PCR mix?? - (reply: 2)
557. How can we design a primer ? step by step method - (reply: 4)
558. Desperate! qPCR standard curve problems - (reply: 2)
559. cDNA synthesis or RT-PCR - (reply: 3)
560. PCR on cDNA and SGamplification??? - (reply: 1)
561. real time PCR protocol - controls? - (reply: 2)
562. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
563. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
564. SOS: new real time PCR machine needed.... which one? - (reply: 5)
565. Amplicon side products - (reply: 5)
566. Fold change graph - (reply: 1)
567. Statistical analyses of 2 - delta delta Ct values - (reply: 2)
568. Finding the right sequence - (reply: 4)
569. Buy BAC vectors - (reply: 1)
570. meaningless real time results - (reply: 1)