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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
541. primer dilutions - (reply: 4)
542. Genotyping PCR primers - (reply: 1)
543. Standard curve using plasmid - (reply: 1)
544. How to compare these results? Please comment. - (reply: 5)
545. Primers too dilute - (reply: 1)
546. Amplification curves jagged - (reply: 1)
547. ddCT issue: no endogenous expression in control. - (reply: 4)
548. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
549. No PCR product in site-directed mutagenesis - (reply: 4)
550. Giardia/Crypto real time PCR late amplification - (reply: 1)
551. How to dilute the template DNA for PCR - (reply: 7)
552. Desired length of overlap region for OE-PCR - (reply: 6)
553. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
554. Need help for PCR - (reply: 5)
555. scorpion probe - (reply: 8)
556. Too low Ct value for reference gene !!! - (reply: 3)
557. unespecific band PCR from mouse genomic DNA - (reply: 9)
558. Help with primer design through PRIMER3 - (reply: 4)
559. Help with unknown band in restriction digest - (reply: 1)
560. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
561. 454 sequencing - (reply: 2)
562. Preference for larger of 2 bands? - (reply: 2)
563. Tm of primers - prediction and verification - (reply: 1)
564. acceptable variation for biological replicate - (reply: 2)
565. RT-PCR contamination in negative control. - (reply: 1)
566. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
567. Technical problem with ABI7300 - (reply: 4)
568. cdna synthesis - (reply: 1)
569. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
570. Help with primer design - (reply: 3)