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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
541. Too low Ct value for reference gene !!! - (reply: 3)
542. unespecific band PCR from mouse genomic DNA - (reply: 9)
543. Help with primer design through PRIMER3 - (reply: 4)
544. Help with unknown band in restriction digest - (reply: 1)
545. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
546. 454 sequencing - (reply: 2)
547. Preference for larger of 2 bands? - (reply: 2)
548. Tm of primers - prediction and verification - (reply: 1)
549. acceptable variation for biological replicate - (reply: 2)
550. RT-PCR contamination in negative control. - (reply: 1)
551. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
552. Technical problem with ABI7300 - (reply: 4)
553. cdna synthesis - (reply: 1)
554. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
555. Help with primer design - (reply: 3)
556. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)
557. PCR smearing and...problems! - (reply: 7)
558. Message Amount in qPCR - (reply: 1)
559. Running Primers on Agarose Gel - (reply: 6)
560. Explanation needed for melting curve - (reply: 3)
561. cDNA concentration of qPCR - (reply: 1)
562. pGEX vector - (reply: 2)
563. will the template of PCR be added A ? - (reply: 2)
564. qPCR normalization of reference gene - (reply: 1)
565. Primer design: Tm above or below Ta? - (reply: 5)
566. Design primers for expression cloning - (reply: 11)
567. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
568. primer dilution query - (reply: 2)
569. Real Time PCR polymerase enzyme - (reply: 3)
570. housekeeping gene has not uniform pattern - (reply: 3)