Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
541. problem with pcr mutagenesis - (reply: 10)
542. PCR primer design - published primers trustable? - (reply: 8)
543. Polymerase for E. coli screenings - (reply: 5)
544. Taqman assay for detecting expression of genes carrying specific SNPs - (reply: 2)
545. complicated RT-PCR issues - (reply: 4)
546. Problem/qPCR, 2 peaks en melting curve - (reply: 4)
547. question about universal and specific primers for miRNA real time pcr - (reply: 1)
548. How to measure expression the mRNA (quantitative) - (reply: 1)
549. Ethanol contamination in RNA - (reply: 11)
550. Normalization of Real time PCR results using Pfaffl method - (reply: 2)
551. RT-PCR vs plate reader for pathogen detection - (reply: 3)
552. How to check primer sequences using BLAST and other tools? - (reply: 2)
553. real time PCR analysis in patient samples - (reply: 2)
554. Experiment plan for RT-qPCR internal reference search - (reply: 3)
555. Primer Check? - (reply: 2)
556. amplification of 14Kb - (reply: 1)
557. Validation of PCR primers/ probes - (reply: 6)
558. genomic DNA extraction from formalin fixed tissues (not embedded) for genotyping - (reply: 4)
559. Real time PCR sudenly not working - (reply: 1)
560. Buffer-composition One-Step RT-PCR - (reply: 4)
561. Two reverse primer sequences for a single forward primer - (reply: 1)
562. Tm variation - (reply: 1)
563. PCR with more than one primer - (reply: 1)
564. RAPD - PCR problem - (reply: 5)
565. Relative fold expression and Normalization fold expression - (reply: 2)
566. Strange Bands - (reply: 2)
567. Recommended tamplate DNA concentration for qPCR - (reply: 2)
568. RT / cDNA synthesis - (reply: 7)
569. How to best store PCR product? - (reply: 2)
570. smearing below band of interest - (reply: 5)