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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
541. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
542. qPCR with several BIOLOGICAL REPLICATES, how to compare? - (reply: 1)
543. issues when designing PCR primers using PRIMER3 - (reply: 8)
544. Primer catalog/databasing - (reply: 1)
545. Nonrepetitive nested PCR - (reply: 2)
546. DNA or cDNA? - (reply: 1)
547. Difference in Ct value of replicates - (reply: 1)
548. TDNA Genotyping PCR program - (reply: 1)
549. Shift in peaks at dissociation curve - (reply: 4)
550. High resolution melting analysis-SNP genotyping. - (reply: 1)
551. PCR - (reply: 2)
552. nonspecific band at 50bp in PCR - (reply: 9)
553. cDNA sequencing - (reply: 2)
554. Maximum PCR product Tm - (reply: 6)
555. QuikChange with Different plasmids - (reply: 1)
556. Good melting curve but non-specific products appeared - (reply: 2)
557. Long Non-Specific PCR Products - (reply: 5)
558. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
559. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
560. fold change (how to represent it) - (reply: 1)
561. PCR - consistent false positive results - (reply: 6)
562. geNorm alternative for qPCR housekeeping genes - (reply: 1)
563. PCR no amplification - (reply: 15)
564. positive NTC in real time reaction - (reply: 12)
565. Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
566. How to quantify virus by PCR - (reply: 12)
567. qPCR contamination problem - (reply: 5)
568. Error rate of commecial reverse transcriptase - (reply: 1)
569. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
570. low A260/230 problem - (reply: 2)