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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
541. PCR - (reply: 2)
542. nonspecific band at 50bp in PCR - (reply: 9)
543. cDNA sequencing - (reply: 2)
544. Maximum PCR product Tm - (reply: 6)
545. QuikChange with Different plasmids - (reply: 1)
546. Good melting curve but non-specific products appeared - (reply: 2)
547. Long Non-Specific PCR Products - (reply: 5)
548. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
549. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
550. fold change (how to represent it) - (reply: 1)
551. PCR - consistent false positive results - (reply: 6)
552. geNorm alternative for qPCR housekeeping genes - (reply: 1)
553. PCR no amplification - (reply: 15)
554. positive NTC in real time reaction - (reply: 12)
555. Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
556. How to quantify virus by PCR - (reply: 12)
557. qPCR contamination problem - (reply: 5)
558. Error rate of commecial reverse transcriptase - (reply: 1)
559. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
560. low A260/230 problem - (reply: 2)
561. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
562. Pcr product size determination for a fusion gene - (reply: 8)
563. open ABI 7500 documents in Macbook - (reply: 1)
564. Primer Annealing Temperatures - (reply: 24)
565. Molecular beacons problem - (reply: 6)
566. RT-qPCR plate set up NoRT controls - (reply: 1)
567. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
568. same primers, different product in conventional PCR and qPCR - (reply: 6)
569. NEB Q5 polymerase works very well - (reply: 1)
570. Plateau height qPCR - (reply: 3)