Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
541. TDNA Genotyping PCR program - (reply: 1)
542. Shift in peaks at dissociation curve - (reply: 4)
543. High resolution melting analysis-SNP genotyping. - (reply: 1)
544. PCR - (reply: 2)
545. nonspecific band at 50bp in PCR - (reply: 9)
546. cDNA sequencing - (reply: 2)
547. Maximum PCR product Tm - (reply: 6)
548. QuikChange with Different plasmids - (reply: 1)
549. Good melting curve but non-specific products appeared - (reply: 2)
550. Long Non-Specific PCR Products - (reply: 5)
551. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
552. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
553. fold change (how to represent it) - (reply: 1)
554. PCR - consistent false positive results - (reply: 6)
555. geNorm alternative for qPCR housekeeping genes - (reply: 1)
556. PCR no amplification - (reply: 15)
557. positive NTC in real time reaction - (reply: 12)
558. Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
559. How to quantify virus by PCR - (reply: 12)
560. qPCR contamination problem - (reply: 5)
561. Error rate of commecial reverse transcriptase - (reply: 1)
562. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
563. low A260/230 problem - (reply: 2)
564. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
565. Pcr product size determination for a fusion gene - (reply: 8)
566. open ABI 7500 documents in Macbook - (reply: 1)
567. Primer Annealing Temperatures - (reply: 24)
568. Molecular beacons problem - (reply: 6)
569. RT-qPCR plate set up NoRT controls - (reply: 1)
570. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)