Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
541. Maximum PCR product Tm - (reply: 6)
542. QuikChange with Different plasmids - (reply: 1)
543. Good melting curve but non-specific products appeared - (reply: 2)
544. Long Non-Specific PCR Products - (reply: 5)
545. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
546. 5'RACE of RNA Pol3 transcripts? - (reply: 3)
547. fold change (how to represent it) - (reply: 1)
548. PCR - consistent false positive results - (reply: 6)
549. geNorm alternative for qPCR housekeeping genes - (reply: 1)
550. PCR no amplification - (reply: 15)
551. positive NTC in real time reaction - (reply: 12)
552. Upscaling cDNA synthesis using SuperScript™ III Reverse Transcriptase - (reply: 4)
553. How to quantify virus by PCR - (reply: 12)
554. qPCR contamination problem - (reply: 5)
555. Error rate of commecial reverse transcriptase - (reply: 1)
556. Why do i get DNA of low concentrate after purification with kit - (reply: 9)
557. low A260/230 problem - (reply: 2)
558. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
559. Pcr product size determination for a fusion gene - (reply: 8)
560. open ABI 7500 documents in Macbook - (reply: 1)
561. Primer Annealing Temperatures - (reply: 24)
562. Molecular beacons problem - (reply: 6)
563. RT-qPCR plate set up NoRT controls - (reply: 1)
564. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
565. same primers, different product in conventional PCR and qPCR - (reply: 6)
566. NEB Q5 polymerase works very well - (reply: 1)
567. Plateau height qPCR - (reply: 3)
568. UDG in qPCR - (reply: 1)
569. 5'RACE - (reply: 3)
570. Taqman Probes problem - (reply: 3)