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New Forum Archives (2009-)
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PCR--RT-PCR-and-Real-Time-PCR
1621.
CORRECT PCR Incorrect RTPCR -
(reply: 10)
1622.
Interpreting melting curve data in Sybr Green RT-PCR -
(reply: 12)
1623.
Confusing bands from PCR - not primer dimer, not product
(reply: 30)
1624.
Real time PCR results-interpretation -
(reply: 2)
1625.
Opinion on Eppendorf Realplex qPCR machin -
(reply: 2)
1626.
Bands appearing out of no where?! -
(reply: 5)
1627.
2-step or 3-step real time PCR - question about real time PCR
(reply: 6)
1628.
SYBR melting curve vs RT-PCR gel -
(reply: 1)
1629.
searching housekeeping genes -
(reply: 4)
1630.
2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes
(reply: 4)
1631.
primer tm - tm calculation for tagged primers
(reply: 2)
1632.
xChIP qPCR calculation if NoAb ctrl is N.A -
(reply: 1)
1633.
Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins?
(reply: 2)
1634.
Long real-time Amplicon -
(reply: 2)
1635.
PCR + phusion enzyme = massive errors -
(reply: 4)
1636.
help needed in cDNA synthesis - needed help desperately,tq
(reply: 3)
1637.
RNA from MMA embedded calcified bone -
(reply: 1)
1638.
long DNA amplification -
(reply: 11)
1639.
same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al
(reply: 2)
1640.
gDNA pcr product as standard for absolute quantification? -
(reply: 1)
1641.
qPCR - no signal detected but plenty of product on gel -
(reply: 3)
1642.
Amplify cDNA for qRT-PCR? -
(reply: 6)
1643.
PCR for cloning - How to perform a PCR for cloning a gene?
(reply: 5)
1644.
QPCR problem! -
(reply: 9)
1645.
pcr trouble solved but I cant seem to understand why -
(reply: 1)
1646.
Primer design if sequence is unknown for organism -
(reply: 5)
1647.
Reference gene qPCR -
(reply: 4)
1648.
Is Taq polymerase still active after staying at 10C for one day? -
(reply: 2)
1649.
Real time PCR info -
(reply: 4)
1650.
Flox and Cre Primers - PCR Troubleshooting - Please Help
(reply: 2)
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