Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1621. Primers annealing temperatures - (reply: 8)
1622. Difficulties cloning by PCR - (reply: 5)
1623. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
1624. rna storage at -20 - (reply: 2)
1625. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
1626. high Cp value PCR - (reply: 2)
1627. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
1628. Synthesize own Oligo-dT primers - (reply: 6)
1629. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
1630. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
1631. dNTP or Deoxynucleotide solution Mix - (reply: 1)
1632. Smearing/ no amplification in PCR - (reply: 3)
1633. qRTPCR (TaqMan) Data Representation - (reply: 1)
1634. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
1635. Lucicferase activity and endogenous transcription - (reply: 7)
1636. need help on primer calcuation soon - (reply: 1)
1637. Heart ache in real time - (reply: 1)
1638. HEX vs. Cy-5 - (reply: 3)
1639. Direct PCR (PCR without DNA extraction) - (reply: 2)
1640. PCR using xt5 - (reply: 2)
1641. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
1642. Problems with qRT-PCR - Lots of unspecific products (reply: 2)
1643. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
1644. 2 bands or smear? 2-step RT-PCR - (reply: 1)
1645. 23S rRNA Primer - need primer sequence (reply: 1)
1646. Lowered extension temps with Phusion - (reply: 7)
1647. RT-PCR - problems with TaqMan PCR (reply: 2)
1648. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
1649. Two annealing temps? - (reply: 4)
1650. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)