|
1621. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1622. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1623. RT-PCR Negative controls - (reply: 1)
1624. Problems with qRT-PCR - (reply: 3)
1625. PCR with 60 bp primers - results in no product (reply: 6)
1626. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1627. How do you manually determine Ct value? - (reply: 1)
1628. Running gel after RT-PCR - (reply: 5)
1629. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1630. Etbr staining methods - (reply: 6)
1631. PCR problems.. - problems with conventional PCR (reply: 3)
1632. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1633. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1634. Dnase digestion and PCR - (reply: 4)
1635. Understanding qPCR and gene expression changes - (reply: 10)
1636. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1637. Reverse Transcription - (reply: 3)
1638. Plasmid as standard - Plasmid calculation (reply: 6)
1639. what Ct values are considered ideal? too high? - (reply: 1)
1640. Need an UV260 RT-PCR instrument - (reply: 1)
1641. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1642. PCR produces products of wrong size - (reply: 4)
1643. PCR with pfu and degenerate primers (?) - (reply: 7)
1644. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1645. Serious issues with RT-PCR - (reply: 7)
1646. relative quantitation problem plz help - (reply: 4)
1647. No beta actin results at all??? - (reply: 1)
1648. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
1649. dNTP question - (reply: 3)
1650. Taqman rtPCR primer and probe design - (reply: 3)
1622. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1623. RT-PCR Negative controls - (reply: 1)
1624. Problems with qRT-PCR - (reply: 3)
1625. PCR with 60 bp primers - results in no product (reply: 6)
1626. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1627. How do you manually determine Ct value? - (reply: 1)
1628. Running gel after RT-PCR - (reply: 5)
1629. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1630. Etbr staining methods - (reply: 6)
1631. PCR problems.. - problems with conventional PCR (reply: 3)
1632. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1633. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1634. Dnase digestion and PCR - (reply: 4)
1635. Understanding qPCR and gene expression changes - (reply: 10)
1636. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1637. Reverse Transcription - (reply: 3)
1638. Plasmid as standard - Plasmid calculation (reply: 6)
1639. what Ct values are considered ideal? too high? - (reply: 1)
1640. Need an UV260 RT-PCR instrument - (reply: 1)
1641. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1642. PCR produces products of wrong size - (reply: 4)
1643. PCR with pfu and degenerate primers (?) - (reply: 7)
1644. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1645. Serious issues with RT-PCR - (reply: 7)
1646. relative quantitation problem plz help - (reply: 4)
1647. No beta actin results at all??? - (reply: 1)
1648. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
1649. dNTP question - (reply: 3)
1650. Taqman rtPCR primer and probe design - (reply: 3)