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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1621. Alternative to netprimer - (reply: 5)
1622. Will ligase buffer affect polymerase fidelity? - (reply: 2)
1623. Primer dimer issue in real time PCR - (reply: 21)
1624. Leaky RT-PCR - (reply: 4)
1625. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
1626. expressing data with more than 1 HK gene - (reply: 2)
1627. RT-PCR primer dimers and cDNA degradation - (reply: 2)
1628. Sequencing of scarce real-time amplicons - (reply: 1)
1629. PCR efficiency important in real time absolute quantification? - (reply: 4)
1630. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
1631. Genotyping PCR - (reply: 3)
1632. Is this standard valid? - pcr product as standards (reply: 5)
1633. geNorm NF calculation - normalization in geNorm (reply: 5)
1634. primer binding - (reply: 1)
1635. Real time 70% efficiency and DDCt - (reply: 1)
1636. RNA visualization - (reply: 1)
1637. PCR primers - (reply: 1)
1638. amplification in negative control in RT PCR - (reply: 2)
1639. 18S changes with cDNA dilution - (reply: 2)
1640. Deletion PCR - (reply: 9)
1641. crossing point - crossing point (reply: 2)
1642. Smear problem with my new primer set - (reply: 2)
1643. PCR ghost bands - (reply: 1)
1644. wash buffer for columns - how to make it? (reply: 3)
1645. amplicon storage - amplicon storage (reply: 3)
1646. CP of real time PCR by LC480 - (reply: 1)
1647. transient expression - best time to detect the mRNA? (reply: 1)
1648. Chosing the best housekeeping gene! - (reply: 5)
1649. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
1650. Unspecific PCR - (reply: 7)