|
1621. Primer3 vs Primer BLAST - (reply: 3)
1622. qPCR using standard curve method - how to do correct setup? (reply: 3)
1623. Primer-Blast - (reply: 3)
1624. Amplification in NTC and noRT controls - (reply: 5)
1625. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
1626. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1627. asymmetric PCR - (reply: 1)
1628. Best time to isolate RNA for qPCR? - (reply: 1)
1629. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
1630. CORRECT PCR Incorrect RTPCR - (reply: 10)
1631. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1632. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1633. Real time PCR results-interpretation - (reply: 2)
1634. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1635. Bands appearing out of no where?! - (reply: 5)
1636. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1637. SYBR melting curve vs RT-PCR gel - (reply: 1)
1638. searching housekeeping genes - (reply: 4)
1639. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1640. primer tm - tm calculation for tagged primers (reply: 2)
1641. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1642. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1643. Long real-time Amplicon - (reply: 2)
1644. PCR + phusion enzyme = massive errors - (reply: 4)
1645. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1646. RNA from MMA embedded calcified bone - (reply: 1)
1647. long DNA amplification - (reply: 11)
1648. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1649. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1650. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1622. qPCR using standard curve method - how to do correct setup? (reply: 3)
1623. Primer-Blast - (reply: 3)
1624. Amplification in NTC and noRT controls - (reply: 5)
1625. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
1626. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1627. asymmetric PCR - (reply: 1)
1628. Best time to isolate RNA for qPCR? - (reply: 1)
1629. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
1630. CORRECT PCR Incorrect RTPCR - (reply: 10)
1631. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1632. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1633. Real time PCR results-interpretation - (reply: 2)
1634. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1635. Bands appearing out of no where?! - (reply: 5)
1636. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1637. SYBR melting curve vs RT-PCR gel - (reply: 1)
1638. searching housekeeping genes - (reply: 4)
1639. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1640. primer tm - tm calculation for tagged primers (reply: 2)
1641. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1642. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1643. Long real-time Amplicon - (reply: 2)
1644. PCR + phusion enzyme = massive errors - (reply: 4)
1645. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1646. RNA from MMA embedded calcified bone - (reply: 1)
1647. long DNA amplification - (reply: 11)
1648. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1649. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1650. qPCR - no signal detected but plenty of product on gel - (reply: 3)