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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1621. transient expression - best time to detect the mRNA? (reply: 1)
1622. Chosing the best housekeeping gene! - (reply: 5)
1623. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
1624. Unspecific PCR - (reply: 7)
1625. Real-Time PCR as a Microplate Reader - (reply: 1)
1626. RT-PCR Internal Standard - (reply: 2)
1627. Differences in Thermal Cyclers? - (reply: 1)
1628. ssDNA Quantification - (reply: 4)
1629. Standards for The Relative Standard Curve Method - (reply: 3)
1630. RNA contamination in PCR - (reply: 1)
1631. DNase I pre-treatment - how can i be sure that it was efficient??? (reply: 2)
1632. early ct value in ntc - (reply: 2)
1633. PCR without DNA extraction! - (reply: 2)
1634. Primer tm - (reply: 1)
1635. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
1636. RT-PCR - (reply: 3)
1637. Long product amplification (1598bp) - PCR for a product of 1598bp length (reply: 3)
1638. PCR problems on a ligation product - (reply: 5)
1639. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
1640. Contamination in negative control of AFLPs (PCRs) - (reply: 1)
1641. Ct fluctuations in qPCR - (reply: 3)
1642. qPCR efficiency - how to improve (reply: 4)
1643. primer dimers - (reply: 5)
1644. are my RT-primers ruined? accidentally stored at 4 C - (reply: 1)
1645. acceptable range for ΔCt ? - (reply: 1)
1646. RT-PCR vs. conventional PCR - (reply: 1)
1647. qPCR primer design - (reply: 5)
1648. Re-PCR problem - (reply: 3)
1649. PCR and gel purification problem - did I screw this up (reply: 4)
1650. touchdown pcr - (reply: 6)