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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1621. Real time PCR results-interpretation - (reply: 2)
1622. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1623. Bands appearing out of no where?! - (reply: 5)
1624. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1625. SYBR melting curve vs RT-PCR gel - (reply: 1)
1626. searching housekeeping genes - (reply: 4)
1627. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1628. primer tm - tm calculation for tagged primers (reply: 2)
1629. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1630. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1631. Long real-time Amplicon - (reply: 2)
1632. PCR + phusion enzyme = massive errors - (reply: 4)
1633. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1634. RNA from MMA embedded calcified bone - (reply: 1)
1635. long DNA amplification - (reply: 11)
1636. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1637. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1638. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1639. Amplify cDNA for qRT-PCR? - (reply: 6)
1640. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1641. QPCR problem! - (reply: 9)
1642. pcr trouble solved but I cant seem to understand why - (reply: 1)
1643. Primer design if sequence is unknown for organism - (reply: 5)
1644. Reference gene qPCR - (reply: 4)
1645. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
1646. Real time PCR info - (reply: 4)
1647. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
1648. standard curve - (reply: 2)
1649. PCR from BAC - PCR from BAC (reply: 1)
1650. Mean versus Mediat Ct Values - Which to use for statistical analsyis (reply: 1)