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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1621. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1622. RNA from MMA embedded calcified bone - (reply: 1)
1623. long DNA amplification - (reply: 11)
1624. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1625. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1626. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1627. Amplify cDNA for qRT-PCR? - (reply: 6)
1628. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1629. QPCR problem! - (reply: 9)
1630. pcr trouble solved but I cant seem to understand why - (reply: 1)
1631. Primer design if sequence is unknown for organism - (reply: 5)
1632. Reference gene qPCR - (reply: 4)
1633. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
1634. Real time PCR info - (reply: 4)
1635. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
1636. standard curve - (reply: 2)
1637. PCR from BAC - PCR from BAC (reply: 1)
1638. Mean versus Mediat Ct Values - Which to use for statistical analsyis (reply: 1)
1639. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
1640. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
1641. heating rna before rt reaction? - (reply: 1)
1642. relative gene expression with multiple reference genes - data analysis (reply: 1)
1643. qRT-PCR no of cycles?? - (reply: 6)
1644. Is there any setting to avoid same base runs in Primer-BLAST? - (reply: 3)
1645. Which techniques should i do? - Which techniques should i do? (reply: 2)
1646. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
1647. what primers should I use for DNA sequencing? please help - (reply: 2)
1648. Standard curve for RT-PCR - (reply: 1)
1649. Problem amplifying plasmid - (reply: 1)
1650. What Factors Affect Ct values of a gene? - (reply: 1)