Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1621. Best time to isolate RNA for qPCR? - (reply: 1)
1622. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
1623. CORRECT PCR Incorrect RTPCR - (reply: 10)
1624. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1625. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1626. Real time PCR results-interpretation - (reply: 2)
1627. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1628. Bands appearing out of no where?! - (reply: 5)
1629. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1630. SYBR melting curve vs RT-PCR gel - (reply: 1)
1631. searching housekeeping genes - (reply: 4)
1632. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1633. primer tm - tm calculation for tagged primers (reply: 2)
1634. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1635. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1636. Long real-time Amplicon - (reply: 2)
1637. PCR + phusion enzyme = massive errors - (reply: 4)
1638. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1639. RNA from MMA embedded calcified bone - (reply: 1)
1640. long DNA amplification - (reply: 11)
1641. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1642. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1643. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1644. Amplify cDNA for qRT-PCR? - (reply: 6)
1645. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1646. QPCR problem! - (reply: 9)
1647. pcr trouble solved but I cant seem to understand why - (reply: 1)
1648. Primer design if sequence is unknown for organism - (reply: 5)
1649. Reference gene qPCR - (reply: 4)
1650. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)