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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1621. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
1622. Problem with absolute quatification - absolute quantification (reply: 1)
1623. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
1624. PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
1625. failed PCR on DNA extract from blood - (reply: 6)
1626. Alternative to netprimer - (reply: 5)
1627. Will ligase buffer affect polymerase fidelity? - (reply: 2)
1628. Primer dimer issue in real time PCR - (reply: 21)
1629. Leaky RT-PCR - (reply: 4)
1630. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
1631. expressing data with more than 1 HK gene - (reply: 2)
1632. RT-PCR primer dimers and cDNA degradation - (reply: 2)
1633. Sequencing of scarce real-time amplicons - (reply: 1)
1634. PCR efficiency important in real time absolute quantification? - (reply: 4)
1635. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
1636. Genotyping PCR - (reply: 3)
1637. Is this standard valid? - pcr product as standards (reply: 5)
1638. geNorm NF calculation - normalization in geNorm (reply: 5)
1639. primer binding - (reply: 1)
1640. Real time 70% efficiency and DDCt - (reply: 1)
1641. RNA visualization - (reply: 1)
1642. PCR primers - (reply: 1)
1643. amplification in negative control in RT PCR - (reply: 2)
1644. 18S changes with cDNA dilution - (reply: 2)
1645. Deletion PCR - (reply: 9)
1646. crossing point - crossing point (reply: 2)
1647. Smear problem with my new primer set - (reply: 2)
1648. PCR ghost bands - (reply: 1)
1649. wash buffer for columns - how to make it? (reply: 3)
1650. amplicon storage - amplicon storage (reply: 3)