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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1621. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
1622. what primers should I use for DNA sequencing? please help - (reply: 2)
1623. Standard curve for RT-PCR - (reply: 1)
1624. Problem amplifying plasmid - (reply: 1)
1625. What Factors Affect Ct values of a gene? - (reply: 1)
1626. Sybr Green I in real-time PCR reaction - (reply: 3)
1627. Primers annealing temperatures - (reply: 8)
1628. Difficulties cloning by PCR - (reply: 5)
1629. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
1630. rna storage at -20 - (reply: 2)
1631. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
1632. high Cp value PCR - (reply: 2)
1633. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
1634. Synthesize own Oligo-dT primers - (reply: 6)
1635. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
1636. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
1637. dNTP or Deoxynucleotide solution Mix - (reply: 1)
1638. Smearing/ no amplification in PCR - (reply: 3)
1639. qRTPCR (TaqMan) Data Representation - (reply: 1)
1640. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
1641. Lucicferase activity and endogenous transcription - (reply: 7)
1642. need help on primer calcuation soon - (reply: 1)
1643. Heart ache in real time - (reply: 1)
1644. HEX vs. Cy-5 - (reply: 3)
1645. Direct PCR (PCR without DNA extraction) - (reply: 2)
1646. PCR using xt5 - (reply: 2)
1647. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
1648. Problems with qRT-PCR - Lots of unspecific products (reply: 2)
1649. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
1650. 2 bands or smear? 2-step RT-PCR - (reply: 1)