Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1621. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1622. Long real-time Amplicon - (reply: 2)
1623. PCR + phusion enzyme = massive errors - (reply: 4)
1624. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1625. RNA from MMA embedded calcified bone - (reply: 1)
1626. long DNA amplification - (reply: 11)
1627. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1628. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1629. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1630. Amplify cDNA for qRT-PCR? - (reply: 6)
1631. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1632. QPCR problem! - (reply: 9)
1633. pcr trouble solved but I cant seem to understand why - (reply: 1)
1634. Primer design if sequence is unknown for organism - (reply: 5)
1635. Reference gene qPCR - (reply: 4)
1636. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
1637. Real time PCR info - (reply: 4)
1638. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
1639. standard curve - (reply: 2)
1640. PCR from BAC - PCR from BAC (reply: 1)
1641. Mean versus Mediat Ct Values - Which to use for statistical analsyis (reply: 1)
1642. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
1643. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
1644. heating rna before rt reaction? - (reply: 1)
1645. relative gene expression with multiple reference genes - data analysis (reply: 1)
1646. qRT-PCR no of cycles?? - (reply: 6)
1647. Is there any setting to avoid same base runs in Primer-BLAST? - (reply: 3)
1648. Which techniques should i do? - Which techniques should i do? (reply: 2)
1649. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
1650. what primers should I use for DNA sequencing? please help - (reply: 2)