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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1621. Sybr Green I in real-time PCR reaction - (reply: 3)
1622. Primers annealing temperatures - (reply: 8)
1623. Difficulties cloning by PCR - (reply: 5)
1624. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
1625. rna storage at -20 - (reply: 2)
1626. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
1627. high Cp value PCR - (reply: 2)
1628. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
1629. Synthesize own Oligo-dT primers - (reply: 6)
1630. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
1631. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
1632. dNTP or Deoxynucleotide solution Mix - (reply: 1)
1633. Smearing/ no amplification in PCR - (reply: 3)
1634. qRTPCR (TaqMan) Data Representation - (reply: 1)
1635. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
1636. Lucicferase activity and endogenous transcription - (reply: 7)
1637. need help on primer calcuation soon - (reply: 1)
1638. Heart ache in real time - (reply: 1)
1639. HEX vs. Cy-5 - (reply: 3)
1640. Direct PCR (PCR without DNA extraction) - (reply: 2)
1641. PCR using xt5 - (reply: 2)
1642. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
1643. Problems with qRT-PCR - Lots of unspecific products (reply: 2)
1644. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
1645. 2 bands or smear? 2-step RT-PCR - (reply: 1)
1646. 23S rRNA Primer - need primer sequence (reply: 1)
1647. Lowered extension temps with Phusion - (reply: 7)
1648. RT-PCR - problems with TaqMan PCR (reply: 2)
1649. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
1650. Two annealing temps? - (reply: 4)