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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1621. relative gene expression with multiple reference genes - data analysis (reply: 1)
1622. qRT-PCR no of cycles?? - (reply: 6)
1623. Is there any setting to avoid same base runs in Primer-BLAST? - (reply: 3)
1624. Which techniques should i do? - Which techniques should i do? (reply: 2)
1625. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
1626. what primers should I use for DNA sequencing? please help - (reply: 2)
1627. Standard curve for RT-PCR - (reply: 1)
1628. Problem amplifying plasmid - (reply: 1)
1629. What Factors Affect Ct values of a gene? - (reply: 1)
1630. Sybr Green I in real-time PCR reaction - (reply: 3)
1631. Primers annealing temperatures - (reply: 8)
1632. Difficulties cloning by PCR - (reply: 5)
1633. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
1634. rna storage at -20 - (reply: 2)
1635. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
1636. high Cp value PCR - (reply: 2)
1637. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
1638. Synthesize own Oligo-dT primers - (reply: 6)
1639. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
1640. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
1641. dNTP or Deoxynucleotide solution Mix - (reply: 1)
1642. Smearing/ no amplification in PCR - (reply: 3)
1643. qRTPCR (TaqMan) Data Representation - (reply: 1)
1644. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
1645. Lucicferase activity and endogenous transcription - (reply: 7)
1646. need help on primer calcuation soon - (reply: 1)
1647. Heart ache in real time - (reply: 1)
1648. HEX vs. Cy-5 - (reply: 3)
1649. Direct PCR (PCR without DNA extraction) - (reply: 2)
1650. PCR using xt5 - (reply: 2)