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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1621. Smearing/ no amplification in PCR - (reply: 3)
1622. qRTPCR (TaqMan) Data Representation - (reply: 1)
1623. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
1624. Lucicferase activity and endogenous transcription - (reply: 7)
1625. need help on primer calcuation soon - (reply: 1)
1626. Heart ache in real time - (reply: 1)
1627. HEX vs. Cy-5 - (reply: 3)
1628. Direct PCR (PCR without DNA extraction) - (reply: 2)
1629. PCR using xt5 - (reply: 2)
1630. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
1631. Problems with qRT-PCR - Lots of unspecific products (reply: 2)
1632. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
1633. 2 bands or smear? 2-step RT-PCR - (reply: 1)
1634. 23S rRNA Primer - need primer sequence (reply: 1)
1635. Lowered extension temps with Phusion - (reply: 7)
1636. RT-PCR - problems with TaqMan PCR (reply: 2)
1637. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
1638. Two annealing temps? - (reply: 4)
1639. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
1640. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
1641. Small product amplification problems - (reply: 4)
1642. ROX and Rotorgene - using a mix with reference dye in Rotorgene (reply: 2)
1643. Freezing primers+SYBR green - (reply: 2)
1644. Tagged Primers - (reply: 5)
1645. no pcr amplification product - (reply: 9)
1646. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
1647. less steep curve and high Ct value! - (reply: 2)
1648. Question about site directed mutagenesis - (reply: 1)
1649. Amplfication of fungal DNA - (reply: 8)
1650. PCR problem - (reply: 4)