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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1621. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
1622. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
1623. heating rna before rt reaction? - (reply: 1)
1624. relative gene expression with multiple reference genes - data analysis (reply: 1)
1625. qRT-PCR no of cycles?? - (reply: 6)
1626. Is there any setting to avoid same base runs in Primer-BLAST? - (reply: 3)
1627. Which techniques should i do? - Which techniques should i do? (reply: 2)
1628. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
1629. what primers should I use for DNA sequencing? please help - (reply: 2)
1630. Standard curve for RT-PCR - (reply: 1)
1631. Problem amplifying plasmid - (reply: 1)
1632. What Factors Affect Ct values of a gene? - (reply: 1)
1633. Sybr Green I in real-time PCR reaction - (reply: 3)
1634. Primers annealing temperatures - (reply: 8)
1635. Difficulties cloning by PCR - (reply: 5)
1636. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
1637. rna storage at -20 - (reply: 2)
1638. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
1639. high Cp value PCR - (reply: 2)
1640. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
1641. Synthesize own Oligo-dT primers - (reply: 6)
1642. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
1643. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
1644. dNTP or Deoxynucleotide solution Mix - (reply: 1)
1645. Smearing/ no amplification in PCR - (reply: 3)
1646. qRTPCR (TaqMan) Data Representation - (reply: 1)
1647. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
1648. Lucicferase activity and endogenous transcription - (reply: 7)
1649. need help on primer calcuation soon - (reply: 1)
1650. Heart ache in real time - (reply: 1)