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New Forum Archives (2009-): :
1621. qPCR using standard curve method - how to do correct setup? (reply: 3)
1623. Amplification in NTC and noRT controls - (reply: 5)
1625. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1627. Best time to isolate RNA for qPCR? - (reply: 1)
1629. CORRECT PCR Incorrect RTPCR - (reply: 10)
1631. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1633. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1635. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1637. searching housekeeping genes - (reply: 4)
1639. primer tm - tm calculation for tagged primers (reply: 2)
1641. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1643. PCR + phusion enzyme = massive errors - (reply: 4)
1645. RNA from MMA embedded calcified bone - (reply: 1)
1647. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1649. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1623. Amplification in NTC and noRT controls - (reply: 5)
1625. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1627. Best time to isolate RNA for qPCR? - (reply: 1)
1629. CORRECT PCR Incorrect RTPCR - (reply: 10)
1631. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1633. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1635. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1637. searching housekeeping genes - (reply: 4)
1639. primer tm - tm calculation for tagged primers (reply: 2)
1641. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1643. PCR + phusion enzyme = massive errors - (reply: 4)
1645. RNA from MMA embedded calcified bone - (reply: 1)
1647. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1649. qPCR - no signal detected but plenty of product on gel - (reply: 3)