|
1621. CORRECT PCR Incorrect RTPCR - (reply: 10)
1622. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1623. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1624. Real time PCR results-interpretation - (reply: 2)
1625. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1626. Bands appearing out of no where?! - (reply: 5)
1627. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1628. SYBR melting curve vs RT-PCR gel - (reply: 1)
1629. searching housekeeping genes - (reply: 4)
1630. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1631. primer tm - tm calculation for tagged primers (reply: 2)
1632. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1633. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1634. Long real-time Amplicon - (reply: 2)
1635. PCR + phusion enzyme = massive errors - (reply: 4)
1636. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1637. RNA from MMA embedded calcified bone - (reply: 1)
1638. long DNA amplification - (reply: 11)
1639. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1640. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1641. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1642. Amplify cDNA for qRT-PCR? - (reply: 6)
1643. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1644. QPCR problem! - (reply: 9)
1645. pcr trouble solved but I cant seem to understand why - (reply: 1)
1646. Primer design if sequence is unknown for organism - (reply: 5)
1647. Reference gene qPCR - (reply: 4)
1648. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
1649. Real time PCR info - (reply: 4)
1650. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
1622. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1623. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1624. Real time PCR results-interpretation - (reply: 2)
1625. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1626. Bands appearing out of no where?! - (reply: 5)
1627. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1628. SYBR melting curve vs RT-PCR gel - (reply: 1)
1629. searching housekeeping genes - (reply: 4)
1630. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1631. primer tm - tm calculation for tagged primers (reply: 2)
1632. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1633. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1634. Long real-time Amplicon - (reply: 2)
1635. PCR + phusion enzyme = massive errors - (reply: 4)
1636. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1637. RNA from MMA embedded calcified bone - (reply: 1)
1638. long DNA amplification - (reply: 11)
1639. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1640. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1641. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1642. Amplify cDNA for qRT-PCR? - (reply: 6)
1643. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1644. QPCR problem! - (reply: 9)
1645. pcr trouble solved but I cant seem to understand why - (reply: 1)
1646. Primer design if sequence is unknown for organism - (reply: 5)
1647. Reference gene qPCR - (reply: 4)
1648. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
1649. Real time PCR info - (reply: 4)
1650. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)