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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1741. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1742. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1743. Dnase digestion and PCR - (reply: 4)
1744. Understanding qPCR and gene expression changes - (reply: 10)
1745. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1746. Reverse Transcription - (reply: 3)
1747. Plasmid as standard - Plasmid calculation (reply: 6)
1748. what Ct values are considered ideal? too high? - (reply: 1)
1749. Need an UV260 RT-PCR instrument - (reply: 1)
1750. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1751. PCR produces products of wrong size - (reply: 4)
1752. PCR with pfu and degenerate primers (?) - (reply: 7)
1753. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1754. Serious issues with RT-PCR - (reply: 7)
1755. relative quantitation problem plz help - (reply: 4)
1756. No beta actin results at all??? - (reply: 1)
1757. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
1758. dNTP question - (reply: 3)
1759. Taqman rtPCR primer and probe design - (reply: 3)
1760. Help with Real Time PCR Well To Well Variation - (reply: 10)
1761. dna for real time - (reply: 3)
1762. Mycobacterial housekeeping genes - (reply: 1)
1763. Expression comparision using Cp values - (reply: 1)
1764. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
1765. Which to use for standard curve? - (reply: 2)
1766. How do you calibrate between runs? - (reply: 3)
1767. Houskeeping Gene not steady - (reply: 7)
1768. real time PCR - (reply: 3)
1769. Real-Time RT-PCR one-step and two step issue - (reply: 13)
1770. Plateau Phase - Taqman not reaching plateau (reply: 1)