Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1741. are my RT-primers ruined? accidentally stored at 4 C - (reply: 1)
1742. acceptable range for ΔCt ? - (reply: 1)
1743. RT-PCR vs. conventional PCR - (reply: 1)
1744. qPCR primer design - (reply: 5)
1745. Re-PCR problem - (reply: 3)
1746. PCR and gel purification problem - did I screw this up (reply: 4)
1747. touchdown pcr - (reply: 6)
1748. non specific pcr products - (reply: 4)
1749. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
1750. template concentration qPCR - (reply: 1)
1751. Anyone with experiences in 2 step PCR - (reply: 1)
1752. qRT-PCR Problems - (reply: 1)
1753. PCR works on lab strain but not patient sample - HIV related (reply: 4)
1754. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
1755. Genomic DNA copy number qPCR - Help with control (reply: 8)
1756. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
1757. re-amplification of a PCR product - is it recommended? (reply: 7)
1758. PCR machine with accurate temperature control? - (reply: 1)
1759. qRT-PCR with DMSO - (reply: 2)
1760. Gene-specific RT-PCR - (reply: 2)
1761. Gain! - (reply: 4)
1762. selection of internal control gene - (reply: 2)
1763. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1764. primer design - primer design question (reply: 2)
1765. Protocol for 16S rDNA real time PCR - (reply: 2)
1766. qPCR - Standard curves (reply: 3)
1767. cDNA spectrophotometric quantification - (reply: 8)
1768. Standard curve standardization - variation between runs (reply: 4)
1769. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1770. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)