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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1741. primer design - primer design question (reply: 2)
1742. Protocol for 16S rDNA real time PCR - (reply: 2)
1743. qPCR - Standard curves (reply: 3)
1744. cDNA spectrophotometric quantification - (reply: 8)
1745. Standard curve standardization - variation between runs (reply: 4)
1746. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1747. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1748. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1749. low gc primers - help with pcr using low gc primers (reply: 6)
1750. Exon-Exon Junction ? - (reply: 3)
1751. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1752. Primers and annealing temperature - (reply: 2)
1753. Bacteria gene expression - (reply: 2)
1754. Copy number calculations in real time PCR - (reply: 2)
1755. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1756. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1757. RT-PCR Negative controls - (reply: 1)
1758. Problems with qRT-PCR - (reply: 3)
1759. PCR with 60 bp primers - results in no product (reply: 6)
1760. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1761. How do you manually determine Ct value? - (reply: 1)
1762. Running gel after RT-PCR - (reply: 5)
1763. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1764. Etbr staining methods - (reply: 6)
1765. PCR problems.. - problems with conventional PCR (reply: 3)
1766. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1767. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1768. Dnase digestion and PCR - (reply: 4)
1769. Understanding qPCR and gene expression changes - (reply: 10)
1770. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)