Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1741. Alternative to netprimer - (reply: 5)
1742. Will ligase buffer affect polymerase fidelity? - (reply: 2)
1743. Primer dimer issue in real time PCR - (reply: 21)
1744. Leaky RT-PCR - (reply: 4)
1745. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
1746. expressing data with more than 1 HK gene - (reply: 2)
1747. RT-PCR primer dimers and cDNA degradation - (reply: 2)
1748. Sequencing of scarce real-time amplicons - (reply: 1)
1749. PCR efficiency important in real time absolute quantification? - (reply: 4)
1750. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
1751. Genotyping PCR - (reply: 3)
1752. Is this standard valid? - pcr product as standards (reply: 5)
1753. geNorm NF calculation - normalization in geNorm (reply: 5)
1754. primer binding - (reply: 1)
1755. Real time 70% efficiency and DDCt - (reply: 1)
1756. RNA visualization - (reply: 1)
1757. PCR primers - (reply: 1)
1758. amplification in negative control in RT PCR - (reply: 2)
1759. 18S changes with cDNA dilution - (reply: 2)
1760. Deletion PCR - (reply: 9)
1761. crossing point - crossing point (reply: 2)
1762. Smear problem with my new primer set - (reply: 2)
1763. PCR ghost bands - (reply: 1)
1764. wash buffer for columns - how to make it? (reply: 3)
1765. amplicon storage - amplicon storage (reply: 3)
1766. CP of real time PCR by LC480 - (reply: 1)
1767. transient expression - best time to detect the mRNA? (reply: 1)
1768. Chosing the best housekeeping gene! - (reply: 5)
1769. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
1770. Unspecific PCR - (reply: 7)