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Top : New Forum Archives (2009-): : PCR, RT-PCR and Real-Time PCR

1741. Taqman rtPCR primer and probe design - (reply: 3)
1742. Help with Real Time PCR Well To Well Variation - (reply: 10)
1743. dna for real time - (reply: 3)
1744. Mycobacterial housekeeping genes - (reply: 1)
1745. Expression comparision using Cp values - (reply: 1)
1746. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
1747. Which to use for standard curve? - (reply: 2)
1748. How do you calibrate between runs? - (reply: 3)
1749. Houskeeping Gene not steady - (reply: 7)
1750. real time PCR - (reply: 3)
1751. Real-Time RT-PCR one-step and two step issue - (reply: 13)
1752. Plateau Phase - Taqman not reaching plateau (reply: 1)
1753. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
1754. maximum amplicon size - 550bp too long??? (reply: 5)
1755. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
1756. SYBR Green miRNA analysis - (reply: 1)
1757. What went wrong in my qPCR? - please look at the dissociation curve (reply: 27)
1758. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
1759. Can DEPC-water interfere QPCR reaction? - (reply: 4)
1760. Melting Curve Analysis Difficulties - (reply: 7)
1761. SYBR and TaqMan - (reply: 6)
1762. real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
1763. New Technology in Real Time PCR - latest technologies (reply: 2)
1764. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
1765. Important literature for real time PCR users - Important documents for beginners as well as advanced users (reply: 41)
1766. PCR efficiencies - (reply: 4)
1767. Optimizing multiplex RT-qPCR - (reply: 3)
1768. difference between hotstart and taq polymerase - (reply: 7)