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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1741. qRT-PCR with DMSO - (reply: 2)
1742. Gene-specific RT-PCR - (reply: 2)
1743. Gain! - (reply: 4)
1744. selection of internal control gene - (reply: 2)
1745. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1746. primer design - primer design question (reply: 2)
1747. Protocol for 16S rDNA real time PCR - (reply: 2)
1748. qPCR - Standard curves (reply: 3)
1749. cDNA spectrophotometric quantification - (reply: 8)
1750. Standard curve standardization - variation between runs (reply: 4)
1751. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1752. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1753. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1754. low gc primers - help with pcr using low gc primers (reply: 6)
1755. Exon-Exon Junction ? - (reply: 3)
1756. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1757. Primers and annealing temperature - (reply: 2)
1758. Bacteria gene expression - (reply: 2)
1759. Copy number calculations in real time PCR - (reply: 2)
1760. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1761. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1762. RT-PCR Negative controls - (reply: 1)
1763. Problems with qRT-PCR - (reply: 3)
1764. PCR with 60 bp primers - results in no product (reply: 6)
1765. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1766. How do you manually determine Ct value? - (reply: 1)
1767. Running gel after RT-PCR - (reply: 5)
1768. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1769. Etbr staining methods - (reply: 6)
1770. PCR problems.. - problems with conventional PCR (reply: 3)