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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1741. RT-PCR Internal Standard - (reply: 2)
1742. Differences in Thermal Cyclers? - (reply: 1)
1743. ssDNA Quantification - (reply: 4)
1744. Standards for The Relative Standard Curve Method - (reply: 3)
1745. RNA contamination in PCR - (reply: 1)
1746. DNase I pre-treatment - how can i be sure that it was efficient??? (reply: 2)
1747. early ct value in ntc - (reply: 2)
1748. PCR without DNA extraction! - (reply: 2)
1749. Primer tm - (reply: 1)
1750. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
1751. RT-PCR - (reply: 3)
1752. Long product amplification (1598bp) - PCR for a product of 1598bp length (reply: 3)
1753. PCR problems on a ligation product - (reply: 5)
1754. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
1755. Contamination in negative control of AFLPs (PCRs) - (reply: 1)
1756. Ct fluctuations in qPCR - (reply: 3)
1757. qPCR efficiency - how to improve (reply: 4)
1758. primer dimers - (reply: 5)
1759. are my RT-primers ruined? accidentally stored at 4 C - (reply: 1)
1760. acceptable range for ΔCt ? - (reply: 1)
1761. RT-PCR vs. conventional PCR - (reply: 1)
1762. qPCR primer design - (reply: 5)
1763. Re-PCR problem - (reply: 3)
1764. PCR and gel purification problem - did I screw this up (reply: 4)
1765. touchdown pcr - (reply: 6)
1766. non specific pcr products - (reply: 4)
1767. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
1768. template concentration qPCR - (reply: 1)
1769. Anyone with experiences in 2 step PCR - (reply: 1)
1770. qRT-PCR Problems - (reply: 1)