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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1741. PCR Cloning-large primers - (reply: 4)
1742. How can I have same plateau fluorescence? - (reply: 1)
1743. Interpreting QPCR DATA - (reply: 3)
1744. what does SQ mean? - (reply: 4)
1745. Free software for oligo analysis - (reply: 3)
1746. Real time house keeping genes change - (reply: 1)
1747. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
1748. Problem with absolute quatification - absolute quantification (reply: 1)
1749. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
1750. PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
1751. failed PCR on DNA extract from blood - (reply: 6)
1752. Alternative to netprimer - (reply: 5)
1753. Will ligase buffer affect polymerase fidelity? - (reply: 2)
1754. Primer dimer issue in real time PCR - (reply: 21)
1755. Leaky RT-PCR - (reply: 4)
1756. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
1757. expressing data with more than 1 HK gene - (reply: 2)
1758. RT-PCR primer dimers and cDNA degradation - (reply: 2)
1759. Sequencing of scarce real-time amplicons - (reply: 1)
1760. PCR efficiency important in real time absolute quantification? - (reply: 4)
1761. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
1762. Genotyping PCR - (reply: 3)
1763. Is this standard valid? - pcr product as standards (reply: 5)
1764. geNorm NF calculation - normalization in geNorm (reply: 5)
1765. primer binding - (reply: 1)
1766. Real time 70% efficiency and DDCt - (reply: 1)
1767. RNA visualization - (reply: 1)
1768. PCR primers - (reply: 1)
1769. amplification in negative control in RT PCR - (reply: 2)
1770. 18S changes with cDNA dilution - (reply: 2)