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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1741. RT-PCR - PCR conditions optimization (reply: 16)
1742. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
1743. way to know if my UNOII pcr machine is working properlly - (reply: 3)
1744. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
1745. denaturation step of melting surve analysis - (reply: 2)
1746. contamination - contamination (reply: 2)
1747. pcr product quantification - (reply: 5)
1748. PCR Sample Prep - (reply: 4)
1749. PCR from roots - I know this sounds a little crazy... (reply: 2)
1750. All gradient samples give bands! How come? - (reply: 4)
1751. 2 Tm peaks with SyBr experiment - (reply: 6)
1752. can mRNA levels and MFI values be correlated - (reply: 3)
1753. PCR Cloning-large primers - (reply: 4)
1754. How can I have same plateau fluorescence? - (reply: 1)
1755. Interpreting QPCR DATA - (reply: 3)
1756. what does SQ mean? - (reply: 4)
1757. Free software for oligo analysis - (reply: 3)
1758. Real time house keeping genes change - (reply: 1)
1759. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
1760. Problem with absolute quatification - absolute quantification (reply: 1)
1761. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
1762. PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
1763. failed PCR on DNA extract from blood - (reply: 6)
1764. Alternative to netprimer - (reply: 5)
1765. Will ligase buffer affect polymerase fidelity? - (reply: 2)
1766. Primer dimer issue in real time PCR - (reply: 21)
1767. Leaky RT-PCR - (reply: 4)
1768. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
1769. expressing data with more than 1 HK gene - (reply: 2)
1770. RT-PCR primer dimers and cDNA degradation - (reply: 2)