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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1741. Exon-Exon Junction ? - (reply: 3)
1742. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1743. Primers and annealing temperature - (reply: 2)
1744. Bacteria gene expression - (reply: 2)
1745. Copy number calculations in real time PCR - (reply: 2)
1746. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1747. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1748. RT-PCR Negative controls - (reply: 1)
1749. Problems with qRT-PCR - (reply: 3)
1750. PCR with 60 bp primers - results in no product (reply: 6)
1751. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1752. How do you manually determine Ct value? - (reply: 1)
1753. Running gel after RT-PCR - (reply: 5)
1754. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1755. Etbr staining methods - (reply: 6)
1756. PCR problems.. - problems with conventional PCR (reply: 3)
1757. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1758. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1759. Dnase digestion and PCR - (reply: 4)
1760. Understanding qPCR and gene expression changes - (reply: 10)
1761. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1762. Reverse Transcription - (reply: 3)
1763. Plasmid as standard - Plasmid calculation (reply: 6)
1764. what Ct values are considered ideal? too high? - (reply: 1)
1765. Need an UV260 RT-PCR instrument - (reply: 1)
1766. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1767. PCR produces products of wrong size - (reply: 4)
1768. PCR with pfu and degenerate primers (?) - (reply: 7)
1769. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1770. Serious issues with RT-PCR - (reply: 7)