Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1741. Real time house keeping genes change - (reply: 1)
1742. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
1743. Problem with absolute quatification - absolute quantification (reply: 1)
1744. RNA isolation for real-time RT-PCR - forgot to inactivate the DNAse (reply: 4)
1745. PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
1746. failed PCR on DNA extract from blood - (reply: 6)
1747. Alternative to netprimer - (reply: 5)
1748. Will ligase buffer affect polymerase fidelity? - (reply: 2)
1749. Primer dimer issue in real time PCR - (reply: 21)
1750. Leaky RT-PCR - (reply: 4)
1751. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
1752. expressing data with more than 1 HK gene - (reply: 2)
1753. RT-PCR primer dimers and cDNA degradation - (reply: 2)
1754. Sequencing of scarce real-time amplicons - (reply: 1)
1755. PCR efficiency important in real time absolute quantification? - (reply: 4)
1756. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
1757. Genotyping PCR - (reply: 3)
1758. Is this standard valid? - pcr product as standards (reply: 5)
1759. geNorm NF calculation - normalization in geNorm (reply: 5)
1760. primer binding - (reply: 1)
1761. Real time 70% efficiency and DDCt - (reply: 1)
1762. RNA visualization - (reply: 1)
1763. PCR primers - (reply: 1)
1764. amplification in negative control in RT PCR - (reply: 2)
1765. 18S changes with cDNA dilution - (reply: 2)
1766. Deletion PCR - (reply: 9)
1767. crossing point - crossing point (reply: 2)
1768. Smear problem with my new primer set - (reply: 2)
1769. PCR ghost bands - (reply: 1)
1770. wash buffer for columns - how to make it? (reply: 3)