Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
721. PCR products form with DNA, but not with c-DNA - (reply: 2)
722. question about pcr amplification efficiency? - (reply: 1)
723. Which Cycler to buy? - Can you recommend any of these cyclers? (reply: 2)
724. a question about the annealing temperature - (reply: 6)
725. what's the function of ROX reference dye? - (reply: 2)
726. Gene Expression Issues - (reply: 3)
727. clear bands on agarose gel but problems in melting curve after qPCR - (reply: 2)
728. No gene amplification, 18s amplification is fine - (reply: 1)
729. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
730. What can I do to increase the banding signal? - (reply: 6)
731. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
732. Tomato Actin PCR+Images included - (reply: 2)
733. single primer PCR - (reply: 5)
734. Weird qPCR curves - (reply: 4)
735. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
736. LacZ PCR Problems - (reply: 1)
737. Primer concentration - (reply: 4)
738. nested PCR with low target DNA? - (reply: 2)
739. RT-PCR calibrator - (reply: 2)
740. problem in pcr - (reply: 2)
741. Can someone recommend me a virtual PCR software? - (reply: 6)
742. negative values after GOI - reference gene ?? - (reply: 4)
743. RNA degradation during electrophoresis?? - (reply: 2)
744. Primer 3' mismatch - Strange experience (reply: 9)
745. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
746. Adding loading/tracking dye to PCR mix? - (reply: 10)
747. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
748. internal control in RT-PCR - inquiry (reply: 2)
749. Melting Temp of Standards Changed? - (reply: 3)
750. DNA polymerase with single G 3'-end overhang activity - (reply: 3)