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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
721. Housekeeping gene for COS-1 cells - (reply: 1)
722. PCR, neg control & no. of cycles - (reply: 2)
723. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
724. efficiency calculation from non-10 fold dilutions - (reply: 1)
725. whole plasmid pcr? - (reply: 6)
726. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
727. Genomic pseudogenes?!? - (reply: 6)
728. Strange amplification plot - (reply: 4)
729. Conventional PCR of different dilutions - (reply: 1)
730. primer resuspension - (reply: 2)
731. Principles of overlap PCR - (reply: 7)
732. Help designing primers with restriction sites - (reply: 3)
733. DNA sequence data came back with lots of wrong bases - (reply: 5)
734. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
735. Abnormal amplification curve - (reply: 1)
736. Problem with Semiquantitative PCR - (reply: 4)
737. false positive/cross reaction with TaqMan - (reply: 1)
738. Problem w/ Genotyping Cre Only - (reply: 3)
739. ROX reference dye for quantitative RT-PCR - (reply: 1)
740. PCR problem - (reply: 9)
741. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
742. why PCR product smeared? - (reply: 2)
743. Tm calculation for primers with RE sites and overhangs - (reply: 4)
744. silincing box, no amplification - (reply: 3)
745. Comparative semiquantitative RT-PCR - (reply: 2)
746. Bands in my PCR Controls - (reply: 3)
747. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)
748. real time qRT-PCR and low expression gene - (reply: 2)
749. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
750. check points while preparing for sequencing - (reply: 10)