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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
721. housekeeping gene has not uniform pattern - (reply: 3)
722. Using BLAST to check primers - (reply: 1)
723. What is the function of a third primer in a PCR mix?? - (reply: 2)
724. How can we design a primer ? step by step method - (reply: 4)
725. Desperate! qPCR standard curve problems - (reply: 2)
726. cDNA synthesis or RT-PCR - (reply: 3)
727. PCR on cDNA and SGamplification??? - (reply: 1)
728. real time PCR protocol - controls? - (reply: 2)
729. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
730. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
731. SOS: new real time PCR machine needed.... which one? - (reply: 5)
732. Amplicon side products - (reply: 5)
733. Fold change graph - (reply: 1)
734. Statistical analyses of 2 - delta delta Ct values - (reply: 2)
735. Finding the right sequence - (reply: 4)
736. Buy BAC vectors - (reply: 1)
737. meaningless real time results - (reply: 1)
738. RT-PCR in a single tube - (reply: 3)
739. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
740. annealing temperature for 3 primer pairs? - (reply: 4)
741. Heated-lid - (reply: 3)
742. DEPC treated water Vs HPLC grade water - (reply: 2)
743. PCR Reaction - (reply: 3)
744. How to interpret this agarose gel? - (reply: 6)
745. low pcr product - (reply: 3)
746. PCR to amplify my insert not working - (reply: 6)
747. HRM analysis, equipment requirements - (reply: 3)
748. AQ vs RQ - (reply: 3)
749. isolation of an already frozen tissue sample - (reply: 1)
750. Problem with PCR amplification- high 3' stability - (reply: 3)