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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
721. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
722. What can I do to increase the banding signal? - (reply: 6)
723. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
724. Tomato Actin PCR+Images included - (reply: 2)
725. single primer PCR - (reply: 5)
726. Weird qPCR curves - (reply: 4)
727. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
728. LacZ PCR Problems - (reply: 1)
729. Primer concentration - (reply: 4)
730. nested PCR with low target DNA? - (reply: 2)
731. RT-PCR calibrator - (reply: 2)
732. problem in pcr - (reply: 2)
733. Can someone recommend me a virtual PCR software? - (reply: 6)
734. negative values after GOI - reference gene ?? - (reply: 4)
735. RNA degradation during electrophoresis?? - (reply: 2)
736. Primer 3' mismatch - Strange experience (reply: 9)
737. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
738. Adding loading/tracking dye to PCR mix? - (reply: 10)
739. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
740. internal control in RT-PCR - inquiry (reply: 2)
741. Melting Temp of Standards Changed? - (reply: 3)
742. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
743. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
744. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
745. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
746. Gene on negative strand - two eductional questions. - (reply: 3)
747. Newbie- choose DNA template - (reply: 1)
748. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
749. Total RNA estimation - (reply: 2)
750. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)