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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
721. Difficult sequences in PCR - (reply: 2)
722. qPCR result interpretation - when your 'relative' is 0?? - (reply: 2)
723. why is real time pcr curve like this? - (reply: 3)
724. Housekeeping gene for COS-1 cells - (reply: 1)
725. PCR, neg control & no. of cycles - (reply: 2)
726. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
727. efficiency calculation from non-10 fold dilutions - (reply: 1)
728. whole plasmid pcr? - (reply: 6)
729. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
730. Genomic pseudogenes?!? - (reply: 6)
731. Strange amplification plot - (reply: 4)
732. Conventional PCR of different dilutions - (reply: 1)
733. primer resuspension - (reply: 2)
734. Principles of overlap PCR - (reply: 7)
735. Help designing primers with restriction sites - (reply: 3)
736. DNA sequence data came back with lots of wrong bases - (reply: 5)
737. Why primers shouldn't have Tm difference greater than 5C? - (reply: 5)
738. Abnormal amplification curve - (reply: 1)
739. Problem with Semiquantitative PCR - (reply: 4)
740. false positive/cross reaction with TaqMan - (reply: 1)
741. Problem w/ Genotyping Cre Only - (reply: 3)
742. ROX reference dye for quantitative RT-PCR - (reply: 1)
743. PCR problem - (reply: 9)
744. Didint get any band during gel electrophoresis even though DNA was there - (reply: 5)
745. why PCR product smeared? - (reply: 2)
746. Tm calculation for primers with RE sites and overhangs - (reply: 4)
747. silincing box, no amplification - (reply: 3)
748. Comparative semiquantitative RT-PCR - (reply: 2)
749. Bands in my PCR Controls - (reply: 3)
750. RTPCR - housekeeping genes: can I use them more intelligently? - (reply: 4)