Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
721. will the template of PCR be added A ? - (reply: 2)
722. qPCR normalization of reference gene - (reply: 1)
723. Primer design: Tm above or below Ta? - (reply: 5)
724. Design primers for expression cloning - (reply: 11)
725. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
726. primer dilution query - (reply: 2)
727. Real Time PCR polymerase enzyme - (reply: 3)
728. housekeeping gene has not uniform pattern - (reply: 3)
729. Using BLAST to check primers - (reply: 1)
730. What is the function of a third primer in a PCR mix?? - (reply: 2)
731. How can we design a primer ? step by step method - (reply: 4)
732. Desperate! qPCR standard curve problems - (reply: 2)
733. cDNA synthesis or RT-PCR - (reply: 3)
734. PCR on cDNA and SGamplification??? - (reply: 1)
735. real time PCR protocol - controls? - (reply: 2)
736. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
737. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
738. SOS: new real time PCR machine needed.... which one? - (reply: 5)
739. Amplicon side products - (reply: 5)
740. Fold change graph - (reply: 1)
741. Statistical analyses of 2 - delta delta Ct values - (reply: 2)
742. Finding the right sequence - (reply: 4)
743. Buy BAC vectors - (reply: 1)
744. meaningless real time results - (reply: 1)
745. RT-PCR in a single tube - (reply: 3)
746. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
747. annealing temperature for 3 primer pairs? - (reply: 4)
748. Heated-lid - (reply: 3)
749. DEPC treated water Vs HPLC grade water - (reply: 2)
750. PCR Reaction - (reply: 3)