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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
211. Inexplicable qPCR failure in single wells - (reply: 10)
212. negative control well 300 bp band - (reply: 3)
213. Reference gene for normalisation - for different growth rates - (reply: 3)
214. Strange amplification plots with high Ct variability - (reply: 1)
215. How big a role does mixing play in PCR - (reply: 1)
216. Melting curve is irregular for primer optimization - (reply: 5)
217. Designing primers for ABO blood groups - (reply: 1)
218. Methylight Results Analysis - (reply: 1)
219. GAPDH Ct value - (reply: 4)
220. dissociation curves - (reply: 1)
221. RT-PCR primer design - (reply: 7)
222. How to amplify very short PCR template - (reply: 4)
223. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
224. Is this primer okay? - (reply: 4)
225. Dilution calculation - (reply: 3)
226. Common causes for low RNA A260/230 ratios - (reply: 7)
227. Limit of detection for salmon gDNA? - (reply: 7)
228. Intensifying signal from positive control - (reply: 5)
229. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
230. analysing qpcr data and plotting standard curve - (reply: 1)
231. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
232. PCR product as standard curve template - (reply: 6)
233. TaqMan CN assay - control sample duplicated - (reply: 1)
234. qPCR - no amplification curve but suitable melting curve - (reply: 3)
235. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
236. Basic question about qPCR and endogenous RNA control. - (reply: 4)
237. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
238. something very basic, why called "event-specific detection" - (reply: 1)
239. skip 65 degrees protocol of Reverse transcription - (reply: 4)
240. Mystery in my PCR - (reply: 5)