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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
211. Amplification curve in the negative control samples - (reply: 7)
212. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
213. Primer as limiting reagent in PCR reaction - (reply: 2)
214. Taqman probe in a LightCycler 480? - (reply: 1)
215. Real time PCR for degraded RNA - (reply: 1)
216. Ct value of housekeeping gene - (reply: 4)
217. Efficiency correct fold change -- use all or none? - (reply: 1)
218. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
219. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
220. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
221. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
222. Is this primer-dimer peak in my melting curve? - (reply: 6)
223. Taq / Phusion mix - (reply: 3)
224. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
225. Dilution series between HKG and GOI - (reply: 7)
226. Reference gene(s) selection and validaton for qPCR - (reply: 5)
227. Is that normal the negative control showing signals? - (reply: 13)
228. qPCR cannot detect reaction. - (reply: 1)
229. website for primer design - (reply: 1)
230. PCR Efficiency over 150%! - (reply: 1)
231. problem of amplification - (reply: 2)
232. Rxn not working - amplify/linearize plasmid - (reply: 3)
233. qRT-PCR works one day, and not the next - (reply: 1)
234. PCR not working - (reply: 11)
235. an urgent problem with replicates - (reply: 10)
236. Site-directed mutagenesis: maximum substitution - (reply: 1)
237. Inter run calibration qRT-PCR - (reply: 2)
238. Primer design and alternative transcripts - (reply: 2)
239. qPCR amplification - (reply: 4)
240. Extremely desperate noob question: How do these PCR work? - (reply: 6)