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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
211. Assay question - (reply: 1)
212. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
213. HRM instrumentaion - (reply: 4)
214. Can you repeat a thermocycle? - (reply: 3)
215. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
216. Amplification curve in the negative control samples - (reply: 7)
217. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
218. Primer as limiting reagent in PCR reaction - (reply: 2)
219. Taqman probe in a LightCycler 480? - (reply: 1)
220. Real time PCR for degraded RNA - (reply: 1)
221. Ct value of housekeeping gene - (reply: 4)
222. Efficiency correct fold change -- use all or none? - (reply: 1)
223. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
224. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
225. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
226. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
227. Is this primer-dimer peak in my melting curve? - (reply: 6)
228. Taq / Phusion mix - (reply: 3)
229. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
230. Dilution series between HKG and GOI - (reply: 7)
231. Reference gene(s) selection and validaton for qPCR - (reply: 5)
232. Is that normal the negative control showing signals? - (reply: 13)
233. qPCR cannot detect reaction. - (reply: 1)
234. website for primer design - (reply: 1)
235. PCR Efficiency over 150%! - (reply: 1)
236. problem of amplification - (reply: 2)
237. Rxn not working - amplify/linearize plasmid - (reply: 3)
238. qRT-PCR works one day, and not the next - (reply: 1)
239. PCR not working - (reply: 11)
240. an urgent problem with replicates - (reply: 10)