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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
211. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
212. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
213. Is this primer-dimer peak in my melting curve? - (reply: 6)
214. Taq / Phusion mix - (reply: 3)
215. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
216. Dilution series between HKG and GOI - (reply: 7)
217. Reference gene(s) selection and validaton for qPCR - (reply: 5)
218. Is that normal the negative control showing signals? - (reply: 13)
219. qPCR cannot detect reaction. - (reply: 1)
220. website for primer design - (reply: 1)
221. PCR Efficiency over 150%! - (reply: 1)
222. problem of amplification - (reply: 2)
223. Rxn not working - amplify/linearize plasmid - (reply: 3)
224. qRT-PCR works one day, and not the next - (reply: 1)
225. PCR not working - (reply: 11)
226. an urgent problem with replicates - (reply: 10)
227. Site-directed mutagenesis: maximum substitution - (reply: 1)
228. Inter run calibration qRT-PCR - (reply: 2)
229. Primer design and alternative transcripts - (reply: 2)
230. qPCR amplification - (reply: 4)
231. Extremely desperate noob question: How do these PCR work? - (reply: 6)
232. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
233. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
234. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
235. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
236. Genomic DNA Isolation from Soft Agar Colonies - (reply: 3)
237. help with total RNA isolation - (reply: 1)
238. Need help to identify bands in AGE result - (reply: 3)
239. COLD-PCR (Dissociation Curve) via qPCR - (reply: 4)
240. how to hasten real-time PCR amplifications - (reply: 2)