Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
631. ddCT issue: no endogenous expression in control. - (reply: 4)
632. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
633. No PCR product in site-directed mutagenesis - (reply: 4)
634. Giardia/Crypto real time PCR late amplification - (reply: 1)
635. How to dilute the template DNA for PCR - (reply: 7)
636. Desired length of overlap region for OE-PCR - (reply: 6)
637. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
638. Need help for PCR - (reply: 5)
639. scorpion probe - (reply: 8)
640. Too low Ct value for reference gene !!! - (reply: 3)
641. unespecific band PCR from mouse genomic DNA - (reply: 9)
642. Help with primer design through PRIMER3 - (reply: 4)
643. Help with unknown band in restriction digest - (reply: 1)
644. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
645. 454 sequencing - (reply: 2)
646. Preference for larger of 2 bands? - (reply: 2)
647. Tm of primers - prediction and verification - (reply: 1)
648. acceptable variation for biological replicate - (reply: 2)
649. RT-PCR contamination in negative control. - (reply: 1)
650. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
651. Technical problem with ABI7300 - (reply: 4)
652. cdna synthesis - (reply: 1)
653. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
654. Help with primer design - (reply: 3)
655. SPUD Assay Standard Curve "bell-shaped" - (reply: 5)
656. PCR smearing and...problems! - (reply: 7)
657. Message Amount in qPCR - (reply: 1)
658. Running Primers on Agarose Gel - (reply: 6)
659. Explanation needed for melting curve - (reply: 3)
660. cDNA concentration of qPCR - (reply: 1)