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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
631. Experiment plan for RT-qPCR internal reference search - (reply: 3)
632. Primer Check? - (reply: 2)
633. amplification of 14Kb - (reply: 1)
634. Validation of PCR primers/ probes - (reply: 6)
635. genomic DNA extraction from formalin fixed tissues (not embedded) for genotyping - (reply: 4)
636. Real time PCR sudenly not working - (reply: 1)
637. Buffer-composition One-Step RT-PCR - (reply: 4)
638. Two reverse primer sequences for a single forward primer - (reply: 1)
639. Tm variation - (reply: 1)
640. PCR with more than one primer - (reply: 1)
641. RAPD - PCR problem - (reply: 5)
642. Relative fold expression and Normalization fold expression - (reply: 2)
643. Strange Bands - (reply: 2)
644. Recommended tamplate DNA concentration for qPCR - (reply: 2)
645. RT / cDNA synthesis - (reply: 7)
646. How to best store PCR product? - (reply: 2)
647. smearing below band of interest - (reply: 5)
648. RT-PCR primer design - (reply: 1)
649. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
650. Odd gel run following PCR - (reply: 28)
651. Qiagen PCR Array Reagents? - (reply: 1)
652. Enough checks for gDNA contamination in qPCR? - (reply: 3)
653. plate stuck in ABI7300 - (reply: 8)
654. No or very weak band using 16s primers - (reply: 1)
655. No PCR amplification with b-actin primers - (reply: 1)
656. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
657. optical qPCR plates - (reply: 1)
658. Fold change significance - (reply: 2)
659. No PCR amplified with long primers - (reply: 10)
660. How many real time-PCR reactions for statistics and how? - (reply: 4)