Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
631. Validation of PCR primers/ probes - (reply: 6)
632. genomic DNA extraction from formalin fixed tissues (not embedded) for genotyping - (reply: 4)
633. Real time PCR sudenly not working - (reply: 1)
634. Buffer-composition One-Step RT-PCR - (reply: 4)
635. Two reverse primer sequences for a single forward primer - (reply: 1)
636. Tm variation - (reply: 1)
637. PCR with more than one primer - (reply: 1)
638. RAPD - PCR problem - (reply: 5)
639. Relative fold expression and Normalization fold expression - (reply: 2)
640. Strange Bands - (reply: 2)
641. Recommended tamplate DNA concentration for qPCR - (reply: 2)
642. RT / cDNA synthesis - (reply: 7)
643. How to best store PCR product? - (reply: 2)
644. smearing below band of interest - (reply: 5)
645. RT-PCR primer design - (reply: 1)
646. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
647. Odd gel run following PCR - (reply: 28)
648. Qiagen PCR Array Reagents? - (reply: 1)
649. Enough checks for gDNA contamination in qPCR? - (reply: 3)
650. plate stuck in ABI7300 - (reply: 8)
651. No or very weak band using 16s primers - (reply: 1)
652. No PCR amplification with b-actin primers - (reply: 1)
653. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
654. optical qPCR plates - (reply: 1)
655. Fold change significance - (reply: 2)
656. No PCR amplified with long primers - (reply: 10)
657. How many real time-PCR reactions for statistics and how? - (reply: 4)
658. PCR optimization: PCR vs qPCR - (reply: 4)
659. PCR smear for genomic samples - (reply: 4)
660. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)