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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
631. Real time data analysis using 2 machines - (reply: 3)
632. Second round of PCR after gel extraction fails miserably - (reply: 3)
633. urgent help plz with RT-PCR - (reply: 3)
634. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
635. LightCycler 480 Melting Curve Assay - (reply: 1)
636. Problems with two step RT-PCR - (reply: 2)
637. Spliced and unspliced form of a gene - (reply: 3)
638. primer dilutions - (reply: 4)
639. Genotyping PCR primers - (reply: 1)
640. Standard curve using plasmid - (reply: 1)
641. How to compare these results? Please comment. - (reply: 5)
642. Primers too dilute - (reply: 1)
643. Amplification curves jagged - (reply: 1)
644. ddCT issue: no endogenous expression in control. - (reply: 4)
645. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
646. No PCR product in site-directed mutagenesis - (reply: 4)
647. Giardia/Crypto real time PCR late amplification - (reply: 1)
648. How to dilute the template DNA for PCR - (reply: 7)
649. Desired length of overlap region for OE-PCR - (reply: 6)
650. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
651. Need help for PCR - (reply: 5)
652. scorpion probe - (reply: 8)
653. Too low Ct value for reference gene !!! - (reply: 3)
654. unespecific band PCR from mouse genomic DNA - (reply: 9)
655. Help with primer design through PRIMER3 - (reply: 4)
656. Help with unknown band in restriction digest - (reply: 1)
657. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
658. 454 sequencing - (reply: 2)
659. Preference for larger of 2 bands? - (reply: 2)
660. Tm of primers - prediction and verification - (reply: 1)