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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
631. primer dilutions - (reply: 4)
632. Genotyping PCR primers - (reply: 1)
633. Standard curve using plasmid - (reply: 1)
634. How to compare these results? Please comment. - (reply: 5)
635. Primers too dilute - (reply: 1)
636. Amplification curves jagged - (reply: 1)
637. ddCT issue: no endogenous expression in control. - (reply: 4)
638. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
639. No PCR product in site-directed mutagenesis - (reply: 4)
640. Giardia/Crypto real time PCR late amplification - (reply: 1)
641. How to dilute the template DNA for PCR - (reply: 7)
642. Desired length of overlap region for OE-PCR - (reply: 6)
643. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
644. Need help for PCR - (reply: 5)
645. scorpion probe - (reply: 8)
646. Too low Ct value for reference gene !!! - (reply: 3)
647. unespecific band PCR from mouse genomic DNA - (reply: 9)
648. Help with primer design through PRIMER3 - (reply: 4)
649. Help with unknown band in restriction digest - (reply: 1)
650. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
651. 454 sequencing - (reply: 2)
652. Preference for larger of 2 bands? - (reply: 2)
653. Tm of primers - prediction and verification - (reply: 1)
654. acceptable variation for biological replicate - (reply: 2)
655. RT-PCR contamination in negative control. - (reply: 1)
656. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
657. Technical problem with ABI7300 - (reply: 4)
658. cdna synthesis - (reply: 1)
659. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
660. Help with primer design - (reply: 3)