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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
631. Primer dimers - (reply: 2)
632. Error bars for qPCR; technical vs. biological replicate - (reply: 2)
633. HaCaT reference gene - (reply: 1)
634. Need help for PCR for AT rich gene - (reply: 3)
635. Amplify fragment in plasmid? - (reply: 4)
636. Relative quantification of different genes in single sample - same threshold val - (reply: 1)
637. Primer validation and PCR efficiency - (reply: 4)
638. How to calculate the price of PCR test per sample? - (reply: 6)
639. dilution help - (reply: 6)
640. rityProbe Integrity in Mastermix during loading 96 well plate - (reply: 2)
641. cloning with nested pcr - (reply: 3)
642. how to find 16srRNA sequences? - (reply: 3)
643. RNA extraction from a 1mm brain tissue punch - uncertain about homogenisation - (reply: 2)
644. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
645. PCR is amplifying non-specific fragment - (reply: 6)
646. Anybody know about temperature sensitivity of the AB TaqMan universal Mastermix - (reply: 4)
647. Measuring of cDNA bands density - (reply: 3)
648. real time RT-PCR problems - (reply: 2)
649. Maximum Annealing-Temperature for Real-Time? - (reply: 3)
650. Real-Time efficiencies and the pfaffl method - (reply: 1)
651. should I design primers spanning both intron and exon - (reply: 2)
652. RT-PCR - (reply: 1)
653. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
654. PCR with a very long and a short primer - (reply: 5)
655. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
656. How to find that my RNA is not contaminated with gDNA, before running Real-Time - (reply: 5)
657. Dilution of 18s. How much? - (reply: 1)
658. Multiplex PCR does not work - (reply: 3)
659. Alternative to Sybr-green? - (reply: 12)
660. Multiple internal controls in using qPCR to measure gene duplication - (reply: 1)