Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
631. another primer dilution question - (reply: 2)
632. TaqMan qPCR low-template issues - (reply: 1)
633. qPCR standard curve slope (m) - (reply: 2)
634. 16s double bands obtained-why?? - (reply: 2)
635. Application of RNase - (reply: 3)
636. i need help with virus pcr - (reply: 2)
637. Primer Efficiency - (reply: 2)
638. data analysis for RT_PCR - (reply: 4)
639. Experimental set up for qRT PCR - (reply: 13)
640. What temp the lid of the thermocycler must be set? - (reply: 1)
641. How should I optimize my PCR - (reply: 2)
642. Real time data analysis using 2 machines - (reply: 3)
643. Second round of PCR after gel extraction fails miserably - (reply: 3)
644. urgent help plz with RT-PCR - (reply: 3)
645. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
646. LightCycler 480 Melting Curve Assay - (reply: 1)
647. Problems with two step RT-PCR - (reply: 2)
648. Spliced and unspliced form of a gene - (reply: 3)
649. primer dilutions - (reply: 4)
650. Genotyping PCR primers - (reply: 1)
651. Standard curve using plasmid - (reply: 1)
652. How to compare these results? Please comment. - (reply: 5)
653. Primers too dilute - (reply: 1)
654. Amplification curves jagged - (reply: 1)
655. ddCT issue: no endogenous expression in control. - (reply: 4)
656. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
657. No PCR product in site-directed mutagenesis - (reply: 4)
658. Giardia/Crypto real time PCR late amplification - (reply: 1)
659. How to dilute the template DNA for PCR - (reply: 7)
660. Desired length of overlap region for OE-PCR - (reply: 6)