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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
631. Primer Efficiency - (reply: 2)
632. data analysis for RT_PCR - (reply: 4)
633. Experimental set up for qRT PCR - (reply: 13)
634. What temp the lid of the thermocycler must be set? - (reply: 1)
635. How should I optimize my PCR - (reply: 2)
636. Real time data analysis using 2 machines - (reply: 3)
637. Second round of PCR after gel extraction fails miserably - (reply: 3)
638. urgent help plz with RT-PCR - (reply: 3)
639. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
640. LightCycler 480 Melting Curve Assay - (reply: 1)
641. Problems with two step RT-PCR - (reply: 2)
642. Spliced and unspliced form of a gene - (reply: 3)
643. primer dilutions - (reply: 4)
644. Genotyping PCR primers - (reply: 1)
645. Standard curve using plasmid - (reply: 1)
646. How to compare these results? Please comment. - (reply: 5)
647. Primers too dilute - (reply: 1)
648. Amplification curves jagged - (reply: 1)
649. ddCT issue: no endogenous expression in control. - (reply: 4)
650. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
651. No PCR product in site-directed mutagenesis - (reply: 4)
652. Giardia/Crypto real time PCR late amplification - (reply: 1)
653. How to dilute the template DNA for PCR - (reply: 7)
654. Desired length of overlap region for OE-PCR - (reply: 6)
655. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
656. Need help for PCR - (reply: 5)
657. scorpion probe - (reply: 8)
658. Too low Ct value for reference gene !!! - (reply: 3)
659. unespecific band PCR from mouse genomic DNA - (reply: 9)
660. Help with primer design through PRIMER3 - (reply: 4)