Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
631. urgent help plz with RT-PCR - (reply: 3)
632. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
633. LightCycler 480 Melting Curve Assay - (reply: 1)
634. Problems with two step RT-PCR - (reply: 2)
635. Spliced and unspliced form of a gene - (reply: 3)
636. primer dilutions - (reply: 4)
637. Genotyping PCR primers - (reply: 1)
638. Standard curve using plasmid - (reply: 1)
639. How to compare these results? Please comment. - (reply: 5)
640. Primers too dilute - (reply: 1)
641. Amplification curves jagged - (reply: 1)
642. ddCT issue: no endogenous expression in control. - (reply: 4)
643. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
644. No PCR product in site-directed mutagenesis - (reply: 4)
645. Giardia/Crypto real time PCR late amplification - (reply: 1)
646. How to dilute the template DNA for PCR - (reply: 7)
647. Desired length of overlap region for OE-PCR - (reply: 6)
648. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
649. Need help for PCR - (reply: 5)
650. scorpion probe - (reply: 8)
651. Too low Ct value for reference gene !!! - (reply: 3)
652. unespecific band PCR from mouse genomic DNA - (reply: 9)
653. Help with primer design through PRIMER3 - (reply: 4)
654. Help with unknown band in restriction digest - (reply: 1)
655. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
656. 454 sequencing - (reply: 2)
657. Preference for larger of 2 bands? - (reply: 2)
658. Tm of primers - prediction and verification - (reply: 1)
659. acceptable variation for biological replicate - (reply: 2)
660. RT-PCR contamination in negative control. - (reply: 1)