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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
631. LightCycler 480 Melting Curve Assay - (reply: 1)
632. Problems with two step RT-PCR - (reply: 2)
633. Spliced and unspliced form of a gene - (reply: 3)
634. primer dilutions - (reply: 4)
635. Genotyping PCR primers - (reply: 1)
636. Standard curve using plasmid - (reply: 1)
637. How to compare these results? Please comment. - (reply: 5)
638. Primers too dilute - (reply: 1)
639. Amplification curves jagged - (reply: 1)
640. ddCT issue: no endogenous expression in control. - (reply: 4)
641. Convering 1:5 serial dilutions to logarithm scale for efficiency curves - (reply: 3)
642. No PCR product in site-directed mutagenesis - (reply: 4)
643. Giardia/Crypto real time PCR late amplification - (reply: 1)
644. How to dilute the template DNA for PCR - (reply: 7)
645. Desired length of overlap region for OE-PCR - (reply: 6)
646. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
647. Need help for PCR - (reply: 5)
648. scorpion probe - (reply: 8)
649. Too low Ct value for reference gene !!! - (reply: 3)
650. unespecific band PCR from mouse genomic DNA - (reply: 9)
651. Help with primer design through PRIMER3 - (reply: 4)
652. Help with unknown band in restriction digest - (reply: 1)
653. how to chose right subsequent for her2(erbb2)gene ? - (reply: 3)
654. 454 sequencing - (reply: 2)
655. Preference for larger of 2 bands? - (reply: 2)
656. Tm of primers - prediction and verification - (reply: 1)
657. acceptable variation for biological replicate - (reply: 2)
658. RT-PCR contamination in negative control. - (reply: 1)
659. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
660. Technical problem with ABI7300 - (reply: 4)