Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1711. differently behavioring replicates in real-time PCR - (reply: 1)
1712. Multiplex PCR - (reply: 1)
1713. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1714. optimal template dna concentrations - (reply: 1)
1715. reconstitution of primers - (reply: 2)
1716. Mysterious PCR Contamination - (reply: 13)
1717. RT-PCR Help - Protocol provided (reply: 4)
1718. Endogenous control in bacterial RT-qPCR - (reply: 4)
1719. Mutagenesis PCR and undesired amplification - (reply: 2)
1720. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1721. primer dimer - (reply: 2)
1722. Genotyping conditioning-where to start? - (reply: 1)
1723. Marker migrates slower depending where is loaded - (reply: 2)
1724. PCR on degraded templates - (reply: 2)
1725. PCR help needed - (reply: 3)
1726. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
1727. Tm difference - (reply: 2)
1728. Genotyping - PCR not working (reply: 6)
1729. RT qPCR Newbie, Weird Results - (reply: 1)
1730. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
1731. light bands, dark smear, dark dimers - (reply: 2)
1732. PCR, followed by sequencing... - why ?? (reply: 3)
1733. qPCR and varying cDNA concentrations - (reply: 1)
1734. PCR program - strange extension step (reply: 2)
1735. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
1736. Trouble with GAPDH housekeeping gene - (reply: 3)
1737. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
1738. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
1739. Using BLAST to check primers - (reply: 17)
1740. qPCR and quantity of RNA - (reply: 2)