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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1711. cDNA spectrophotometric quantification - (reply: 8)
1712. Standard curve standardization - variation between runs (reply: 4)
1713. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1714. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1715. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1716. low gc primers - help with pcr using low gc primers (reply: 6)
1717. Exon-Exon Junction ? - (reply: 3)
1718. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1719. Primers and annealing temperature - (reply: 2)
1720. Bacteria gene expression - (reply: 2)
1721. Copy number calculations in real time PCR - (reply: 2)
1722. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1723. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1724. RT-PCR Negative controls - (reply: 1)
1725. Problems with qRT-PCR - (reply: 3)
1726. PCR with 60 bp primers - results in no product (reply: 6)
1727. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1728. How do you manually determine Ct value? - (reply: 1)
1729. Running gel after RT-PCR - (reply: 5)
1730. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1731. Etbr staining methods - (reply: 6)
1732. PCR problems.. - problems with conventional PCR (reply: 3)
1733. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1734. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1735. Dnase digestion and PCR - (reply: 4)
1736. Understanding qPCR and gene expression changes - (reply: 10)
1737. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1738. Reverse Transcription - (reply: 3)
1739. Plasmid as standard - Plasmid calculation (reply: 6)
1740. what Ct values are considered ideal? too high? - (reply: 1)