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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1711. Endogenous control in bacterial RT-qPCR - (reply: 4)
1712. Mutagenesis PCR and undesired amplification - (reply: 2)
1713. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1714. primer dimer - (reply: 2)
1715. Genotyping conditioning-where to start? - (reply: 1)
1716. Marker migrates slower depending where is loaded - (reply: 2)
1717. PCR on degraded templates - (reply: 2)
1718. PCR help needed - (reply: 3)
1719. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
1720. Tm difference - (reply: 2)
1721. Genotyping - PCR not working (reply: 6)
1722. RT qPCR Newbie, Weird Results - (reply: 1)
1723. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
1724. light bands, dark smear, dark dimers - (reply: 2)
1725. PCR, followed by sequencing... - why ?? (reply: 3)
1726. qPCR and varying cDNA concentrations - (reply: 1)
1727. PCR program - strange extension step (reply: 2)
1728. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
1729. Trouble with GAPDH housekeeping gene - (reply: 3)
1730. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
1731. qPCR lacks reproduceability - perfect runs, no reproduceability, no explaination, desperate... (reply: 5)
1732. Using BLAST to check primers - (reply: 17)
1733. qPCR and quantity of RNA - (reply: 2)
1734. pcr doesnt show any band for DNa walking - (reply: 8)
1735. How & where to prepare qPCR - (reply: 9)
1736. about qPCR plates sealing films - Axygen UC-500 vs. UC-200 (reply: 4)
1737. storing large amounts of PCR mastermix beforehand - question (reply: 3)
1738. questions about semi-quantitive PCR - weird result form semi-quantitive PCR (reply: 2)
1739. Effects of more cDNA in PCR??? - (reply: 1)
1740. PCR product disappears after restriction digestion - (reply: 4)