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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1711. Long product amplification (1598bp) - PCR for a product of 1598bp length (reply: 3)
1712. PCR problems on a ligation product - (reply: 5)
1713. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
1714. Contamination in negative control of AFLPs (PCRs) - (reply: 1)
1715. Ct fluctuations in qPCR - (reply: 3)
1716. qPCR efficiency - how to improve (reply: 4)
1717. primer dimers - (reply: 5)
1718. are my RT-primers ruined? accidentally stored at 4 C - (reply: 1)
1719. acceptable range for ΔCt ? - (reply: 1)
1720. RT-PCR vs. conventional PCR - (reply: 1)
1721. qPCR primer design - (reply: 5)
1722. Re-PCR problem - (reply: 3)
1723. PCR and gel purification problem - did I screw this up (reply: 4)
1724. touchdown pcr - (reply: 6)
1725. non specific pcr products - (reply: 4)
1726. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
1727. template concentration qPCR - (reply: 1)
1728. Anyone with experiences in 2 step PCR - (reply: 1)
1729. qRT-PCR Problems - (reply: 1)
1730. PCR works on lab strain but not patient sample - HIV related (reply: 4)
1731. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
1732. Genomic DNA copy number qPCR - Help with control (reply: 8)
1733. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
1734. re-amplification of a PCR product - is it recommended? (reply: 7)
1735. PCR machine with accurate temperature control? - (reply: 1)
1736. qRT-PCR with DMSO - (reply: 2)
1737. Gene-specific RT-PCR - (reply: 2)
1738. Gain! - (reply: 4)
1739. selection of internal control gene - (reply: 2)
1740. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)