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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1711. RT-PCR vs. conventional PCR - (reply: 1)
1712. qPCR primer design - (reply: 5)
1713. Re-PCR problem - (reply: 3)
1714. PCR and gel purification problem - did I screw this up (reply: 4)
1715. touchdown pcr - (reply: 6)
1716. non specific pcr products - (reply: 4)
1717. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
1718. template concentration qPCR - (reply: 1)
1719. Anyone with experiences in 2 step PCR - (reply: 1)
1720. qRT-PCR Problems - (reply: 1)
1721. PCR works on lab strain but not patient sample - HIV related (reply: 4)
1722. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
1723. Genomic DNA copy number qPCR - Help with control (reply: 8)
1724. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
1725. re-amplification of a PCR product - is it recommended? (reply: 7)
1726. PCR machine with accurate temperature control? - (reply: 1)
1727. qRT-PCR with DMSO - (reply: 2)
1728. Gene-specific RT-PCR - (reply: 2)
1729. Gain! - (reply: 4)
1730. selection of internal control gene - (reply: 2)
1731. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1732. primer design - primer design question (reply: 2)
1733. Protocol for 16S rDNA real time PCR - (reply: 2)
1734. qPCR - Standard curves (reply: 3)
1735. cDNA spectrophotometric quantification - (reply: 8)
1736. Standard curve standardization - variation between runs (reply: 4)
1737. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1738. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1739. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1740. low gc primers - help with pcr using low gc primers (reply: 6)