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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1711. qRT-PCR Problems - (reply: 1)
1712. PCR works on lab strain but not patient sample - HIV related (reply: 4)
1713. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
1714. Genomic DNA copy number qPCR - Help with control (reply: 8)
1715. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
1716. re-amplification of a PCR product - is it recommended? (reply: 7)
1717. PCR machine with accurate temperature control? - (reply: 1)
1718. qRT-PCR with DMSO - (reply: 2)
1719. Gene-specific RT-PCR - (reply: 2)
1720. Gain! - (reply: 4)
1721. selection of internal control gene - (reply: 2)
1722. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1723. primer design - primer design question (reply: 2)
1724. Protocol for 16S rDNA real time PCR - (reply: 2)
1725. qPCR - Standard curves (reply: 3)
1726. cDNA spectrophotometric quantification - (reply: 8)
1727. Standard curve standardization - variation between runs (reply: 4)
1728. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1729. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
1730. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1731. low gc primers - help with pcr using low gc primers (reply: 6)
1732. Exon-Exon Junction ? - (reply: 3)
1733. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1734. Primers and annealing temperature - (reply: 2)
1735. Bacteria gene expression - (reply: 2)
1736. Copy number calculations in real time PCR - (reply: 2)
1737. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1738. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1739. RT-PCR Negative controls - (reply: 1)
1740. Problems with qRT-PCR - (reply: 3)