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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1711. PCR with 60 bp primers - results in no product (reply: 6)
1712. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1713. How do you manually determine Ct value? - (reply: 1)
1714. Running gel after RT-PCR - (reply: 5)
1715. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1716. Etbr staining methods - (reply: 6)
1717. PCR problems.. - problems with conventional PCR (reply: 3)
1718. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1719. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1720. Dnase digestion and PCR - (reply: 4)
1721. Understanding qPCR and gene expression changes - (reply: 10)
1722. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1723. Reverse Transcription - (reply: 3)
1724. Plasmid as standard - Plasmid calculation (reply: 6)
1725. what Ct values are considered ideal? too high? - (reply: 1)
1726. Need an UV260 RT-PCR instrument - (reply: 1)
1727. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1728. PCR produces products of wrong size - (reply: 4)
1729. PCR with pfu and degenerate primers (?) - (reply: 7)
1730. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1731. Serious issues with RT-PCR - (reply: 7)
1732. relative quantitation problem plz help - (reply: 4)
1733. No beta actin results at all??? - (reply: 1)
1734. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
1735. dNTP question - (reply: 3)
1736. Taqman rtPCR primer and probe design - (reply: 3)
1737. Help with Real Time PCR Well To Well Variation - (reply: 10)
1738. dna for real time - (reply: 3)
1739. Mycobacterial housekeeping genes - (reply: 1)
1740. Expression comparision using Cp values - (reply: 1)