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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1711. Copy number calculations in real time PCR - (reply: 2)
1712. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
1713. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
1714. RT-PCR Negative controls - (reply: 1)
1715. Problems with qRT-PCR - (reply: 3)
1716. PCR with 60 bp primers - results in no product (reply: 6)
1717. Shrimp nuclease to eliminate genomic DNA - (reply: 7)
1718. How do you manually determine Ct value? - (reply: 1)
1719. Running gel after RT-PCR - (reply: 5)
1720. What quantitation method you prefer to use in relative qPCR? - Standard curve, Pfaffl, deltadelta CT? (reply: 3)
1721. Etbr staining methods - (reply: 6)
1722. PCR problems.. - problems with conventional PCR (reply: 3)
1723. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
1724. gDNA or cDNA amplification in RT-PCR - (reply: 3)
1725. Dnase digestion and PCR - (reply: 4)
1726. Understanding qPCR and gene expression changes - (reply: 10)
1727. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
1728. Reverse Transcription - (reply: 3)
1729. Plasmid as standard - Plasmid calculation (reply: 6)
1730. what Ct values are considered ideal? too high? - (reply: 1)
1731. Need an UV260 RT-PCR instrument - (reply: 1)
1732. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
1733. PCR produces products of wrong size - (reply: 4)
1734. PCR with pfu and degenerate primers (?) - (reply: 7)
1735. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
1736. Serious issues with RT-PCR - (reply: 7)
1737. relative quantitation problem plz help - (reply: 4)
1738. No beta actin results at all??? - (reply: 1)
1739. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
1740. dNTP question - (reply: 3)