Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1711. CP of real time PCR by LC480 - (reply: 1)
1712. transient expression - best time to detect the mRNA? (reply: 1)
1713. Chosing the best housekeeping gene! - (reply: 5)
1714. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
1715. Unspecific PCR - (reply: 7)
1716. Real-Time PCR as a Microplate Reader - (reply: 1)
1717. RT-PCR Internal Standard - (reply: 2)
1718. Differences in Thermal Cyclers? - (reply: 1)
1719. ssDNA Quantification - (reply: 4)
1720. Standards for The Relative Standard Curve Method - (reply: 3)
1721. RNA contamination in PCR - (reply: 1)
1722. DNase I pre-treatment - how can i be sure that it was efficient??? (reply: 2)
1723. early ct value in ntc - (reply: 2)
1724. PCR without DNA extraction! - (reply: 2)
1725. Primer tm - (reply: 1)
1726. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
1727. RT-PCR - (reply: 3)
1728. Long product amplification (1598bp) - PCR for a product of 1598bp length (reply: 3)
1729. PCR problems on a ligation product - (reply: 5)
1730. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
1731. Contamination in negative control of AFLPs (PCRs) - (reply: 1)
1732. Ct fluctuations in qPCR - (reply: 3)
1733. qPCR efficiency - how to improve (reply: 4)
1734. primer dimers - (reply: 5)
1735. are my RT-primers ruined? accidentally stored at 4 C - (reply: 1)
1736. acceptable range for ΔCt ? - (reply: 1)
1737. RT-PCR vs. conventional PCR - (reply: 1)
1738. qPCR primer design - (reply: 5)
1739. Re-PCR problem - (reply: 3)
1740. PCR and gel purification problem - did I screw this up (reply: 4)