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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1201. Log 10 fold serial dilutions - (reply: 2)
1202. paranormal qpcr amplification activity - melting curves (reply: 3)
1203. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
1204. PCR template concentration - (reply: 6)
1205. PCR efficiency calculated by Linreg - (reply: 3)
1206. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
1207. Stability of genomic DNA in 1x PBS - (reply: 2)
1208. primer design by generunner DG - (reply: 1)
1209. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
1210. degenerate pcr - (reply: 1)
1211. DNA degradation - (reply: 1)
1212. DNase 1 treratment of RNA - (reply: 11)
1213. Secondary structure in real time - (reply: 1)
1214. primers with fluoresent dye PCR cycle question - (reply: 4)
1215. House keeping gene question? - Thanks for any help! (reply: 1)
1216. Questions about Tween 20 - (reply: 1)
1217. General qRT-PCR question - (reply: 3)
1218. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
1219. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
1220. Smears on PCR products - (reply: 4)
1221. Dumb Reverse Transcription PCR Question - (reply: 2)
1222. qPCR absolute/relative quantification method question - (reply: 4)
1223. PCR-amplicon too short - (reply: 1)
1224. Reverse transcription PCR - (reply: 1)
1225. PCR/RT-PCR beads - (reply: 2)
1226. High Amplification Efficiency in Std. Curve - (reply: 5)
1227. Run PCR amplifications in agarose gels - (reply: 3)
1228. Problems with validating primers and low expression genes - (reply: 3)
1229. Compare different qPCR runs - really need help (reply: 8)
1230. General real time questions. - (reply: 1)