Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1201. PCR contamination - (reply: 19)
1202. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1203. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1204. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1205. PCR not working - No amplification - (reply: 5)
1206. Quantification of PCR products - (reply: 1)
1207. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)
1208. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1209. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1210. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1211. Nested PCR question - (reply: 1)
1212. High Tm on primers - cannot get a product - (reply: 8)
1213. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1214. Help with Multiplex Nested PCR - (reply: 2)
1215. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1216. trembling dissociation curves in HRM assays - (reply: 3)
1217. Fluorescein storage? - (reply: 2)
1218. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1219. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1220. TELO2 & GAPDH - (reply: 2)
1221. PCR -No band formation - (reply: 1)
1222. 3' race - (reply: 1)
1223. PCR AMPLIFICATION - (reply: 1)
1224. internal control for genomic DNA - (reply: 1)
1225. qPCR Data Analysis - Software (reply: 3)
1226. Gene expression confusion - (reply: 5)
1227. real time pcr need advise - (reply: 2)
1228. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1229. ChIP-qPCR shift in melt curve - (reply: 4)
1230. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)