Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1201. PCR reaction without template gives a product - (reply: 6)
1202. PCR wrong product size - (reply: 6)
1203. PCR very faint product - (reply: 1)
1204. proteinase k inhibition/inactivation - method... (reply: 2)
1205. What does melting temperature indicate and how to predict annealing temperature - (reply: 9)
1206. 4 question for real time PCR - (reply: 5)
1207. RNA quality - (reply: 3)
1208. Superscript III - No second strand synthesis? - (reply: 1)
1209. cDNA convertion to DNA - (reply: 4)
1210. PCR didn't work - suspect the enzyme (reply: 2)
1211. How to check RNA quality - (reply: 5)
1212. Colony PCR Specificity - (reply: 2)
1213. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
1214. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 10)
1215. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
1216. Primer Dilution Problem - D'oh! (reply: 2)
1217. Using PCR to create overhangs - (reply: 3)
1218. Site directed mutation - Changing D->K (reply: 2)
1219. Miner groove binder - urgent, help, what is it? (reply: 4)
1220. Problem with PCR on bacmids - (reply: 1)
1221. Copy number assay using Real time Pcr-SYBR green assay? - (reply: 3)
1222. FAM/HEX, lightcyler 480 - (reply: 1)
1223. Running real time pcr product on gel?.. - (reply: 4)
1224. help with ct of reference gene - (reply: 1)
1225. PCR mutagenesis of plasmid - (reply: 5)
1226. Primer designed for ARMS PCR - (reply: 2)
1227. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
1228. methylation specific PCR - Primers - (reply: 1)
1229. bacterial identification using real time PCR - (reply: 1)
1230. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)