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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1201. Primer designed for ARMS PCR - (reply: 2)
1202. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
1203. methylation specific PCR - Primers - (reply: 1)
1204. bacterial identification using real time PCR - (reply: 1)
1205. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
1206. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
1207. Problem with multiplex PCR - Troubleshooting (reply: 4)
1208. Questions about ChIP protocols - (reply: 1)
1209. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
1210. What’s wrong with my NTC - (reply: 2)
1211. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
1212. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
1213. PCR reamplification - (reply: 2)
1214. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
1215. question about pcr - (reply: 3)
1216. Standarization of HRM for methylation - (reply: 4)
1217. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
1218. How to construct a standard curve for real time PCR - (reply: 1)
1219. PCR with one primer - (reply: 1)
1220. cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
1221. First strand synthesis - (reply: 1)
1222. PCR contamination - (reply: 19)
1223. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1224. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1225. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1226. PCR not working - No amplification - (reply: 5)
1227. Quantification of PCR products - (reply: 1)
1228. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)
1229. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1230. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)