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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1201. bacterial identification using real time PCR - (reply: 1)
1202. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
1203. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
1204. Problem with multiplex PCR - Troubleshooting (reply: 4)
1205. Questions about ChIP protocols - (reply: 1)
1206. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
1207. What’s wrong with my NTC - (reply: 2)
1208. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
1209. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
1210. PCR reamplification - (reply: 2)
1211. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
1212. question about pcr - (reply: 3)
1213. Standarization of HRM for methylation - (reply: 4)
1214. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
1215. How to construct a standard curve for real time PCR - (reply: 1)
1216. PCR with one primer - (reply: 1)
1217. cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
1218. First strand synthesis - (reply: 1)
1219. PCR contamination - (reply: 19)
1220. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1221. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1222. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1223. PCR not working - No amplification - (reply: 5)
1224. Quantification of PCR products - (reply: 1)
1225. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)
1226. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1227. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1228. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1229. Nested PCR question - (reply: 1)
1230. High Tm on primers - cannot get a product - (reply: 8)