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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1201. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
1202. Smears on PCR products - (reply: 4)
1203. Dumb Reverse Transcription PCR Question - (reply: 2)
1204. qPCR absolute/relative quantification method question - (reply: 4)
1205. PCR-amplicon too short - (reply: 1)
1206. Reverse transcription PCR - (reply: 1)
1207. PCR/RT-PCR beads - (reply: 2)
1208. High Amplification Efficiency in Std. Curve - (reply: 5)
1209. Run PCR amplifications in agarose gels - (reply: 3)
1210. Problems with validating primers and low expression genes - (reply: 3)
1211. Compare different qPCR runs - really need help (reply: 8)
1212. General real time questions. - (reply: 1)
1213. Analysis HELP!!!! - (reply: 1)
1214. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1215. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1216. Mutagenesis woes - (reply: 3)
1217. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1218. intron/exon spanning primers - (reply: 1)
1219. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
1220. PCR product for sequencing - sample storage?? (reply: 2)
1221. Absolute quantification using Real Time PCR - (reply: 2)
1222. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
1223. High Ct value - what to do next? - (reply: 4)
1224. Biorad Mini Opticon - can use any 0.2ml tubes or Biorad only? (reply: 1)
1225. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
1226. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
1227. Designing Primers for multiple Isoforms - (reply: 1)
1228. GAPDH CT lower than target gene CT - (reply: 2)
1229. standards for absolute quantification qPCR - (reply: 1)
1230. RT-PCR standard curve dilutions - (reply: 2)