Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1201. What’s wrong with my NTC - (reply: 2)
1202. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
1203. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
1204. PCR reamplification - (reply: 2)
1205. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
1206. question about pcr - (reply: 3)
1207. Standarization of HRM for methylation - (reply: 4)
1208. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
1209. How to construct a standard curve for real time PCR - (reply: 1)
1210. PCR with one primer - (reply: 1)
1211. cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
1212. First strand synthesis - (reply: 1)
1213. PCR contamination - (reply: 19)
1214. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1215. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1216. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1217. PCR not working - No amplification - (reply: 5)
1218. Quantification of PCR products - (reply: 1)
1219. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)
1220. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1221. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1222. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1223. Nested PCR question - (reply: 1)
1224. High Tm on primers - cannot get a product - (reply: 8)
1225. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1226. Help with Multiplex Nested PCR - (reply: 2)
1227. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1228. trembling dissociation curves in HRM assays - (reply: 3)
1229. Fluorescein storage? - (reply: 2)
1230. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)