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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1201. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
1202. Stability of genomic DNA in 1x PBS - (reply: 2)
1203. primer design by generunner DG - (reply: 1)
1204. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
1205. degenerate pcr - (reply: 1)
1206. DNA degradation - (reply: 1)
1207. DNase 1 treratment of RNA - (reply: 11)
1208. Secondary structure in real time - (reply: 1)
1209. primers with fluoresent dye PCR cycle question - (reply: 4)
1210. House keeping gene question? - Thanks for any help! (reply: 1)
1211. Questions about Tween 20 - (reply: 1)
1212. General qRT-PCR question - (reply: 3)
1213. qPCR stats problem - Vandesompele's, DDct or other methods?? (reply: 1)
1214. Taqman probe not easily synthesized - What sequence features make probe synthesis difficult? (reply: 3)
1215. Smears on PCR products - (reply: 4)
1216. Dumb Reverse Transcription PCR Question - (reply: 2)
1217. qPCR absolute/relative quantification method question - (reply: 4)
1218. PCR-amplicon too short - (reply: 1)
1219. Reverse transcription PCR - (reply: 1)
1220. PCR/RT-PCR beads - (reply: 2)
1221. High Amplification Efficiency in Std. Curve - (reply: 5)
1222. Run PCR amplifications in agarose gels - (reply: 3)
1223. Problems with validating primers and low expression genes - (reply: 3)
1224. Compare different qPCR runs - really need help (reply: 8)
1225. General real time questions. - (reply: 1)
1226. Analysis HELP!!!! - (reply: 1)
1227. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1228. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1229. Mutagenesis woes - (reply: 3)
1230. KpnI Restriction enzyme - how to detect the SNP (reply: 1)