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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1201. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
1202. colony PCR - (reply: 2)
1203. copy number variation - (reply: 3)
1204. How to deal with truncated mRNA or mRNA that has secondary structure - (reply: 2)
1205. Very weird gel - I cannot explain these results (reply: 11)
1206. well position effects (edge and corner) effects in qPCR - using Bio-Rad iCycler iQ (reply: 2)
1207. Cytosolic Housekeeping gene - (reply: 1)
1208. PCR reaction without template gives a product - (reply: 6)
1209. PCR wrong product size - (reply: 6)
1210. PCR very faint product - (reply: 1)
1211. proteinase k inhibition/inactivation - method... (reply: 2)
1212. What does melting temperature indicate and how to predict annealing temperature - (reply: 9)
1213. 4 question for real time PCR - (reply: 5)
1214. RNA quality - (reply: 3)
1215. Superscript III - No second strand synthesis? - (reply: 1)
1216. cDNA convertion to DNA - (reply: 4)
1217. PCR didn't work - suspect the enzyme (reply: 2)
1218. How to check RNA quality - (reply: 5)
1219. Colony PCR Specificity - (reply: 2)
1220. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
1221. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 10)
1222. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
1223. Primer Dilution Problem - D'oh! (reply: 2)
1224. Using PCR to create overhangs - (reply: 3)
1225. Site directed mutation - Changing D->K (reply: 2)
1226. Miner groove binder - urgent, help, what is it? (reply: 4)
1227. Problem with PCR on bacmids - (reply: 1)
1228. Copy number assay using Real time Pcr-SYBR green assay? - (reply: 3)
1229. FAM/HEX, lightcyler 480 - (reply: 1)
1230. Running real time pcr product on gel?.. - (reply: 4)