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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1201. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1202. Mutagenesis woes - (reply: 3)
1203. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1204. intron/exon spanning primers - (reply: 1)
1205. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
1206. PCR product for sequencing - sample storage?? (reply: 2)
1207. Absolute quantification using Real Time PCR - (reply: 2)
1208. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
1209. High Ct value - what to do next? - (reply: 4)
1210. Biorad Mini Opticon - can use any 0.2ml tubes or Biorad only? (reply: 1)
1211. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
1212. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
1213. Designing Primers for multiple Isoforms - (reply: 1)
1214. GAPDH CT lower than target gene CT - (reply: 2)
1215. standards for absolute quantification qPCR - (reply: 1)
1216. RT-PCR standard curve dilutions - (reply: 2)
1217. more than one band - (reply: 1)
1218. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
1219. qPCR, RT- Control - (reply: 2)
1220. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
1221. real time pcr melt curve and primer efficiency problem - (reply: 3)
1222. real time PCR - base line not zero anymore! (reply: 5)
1223. Pre-amplification of template for qPCR - (reply: 1)
1224. Efficiency estimation using a window of linearity - (reply: 3)
1225. Software for analysis of raw real-time flouresence data - Analysis of tab delimited text flouresence v cycle data (reply: 7)
1226. Real time PCR - Standard curve construction (reply: 2)
1227. issue with taqman MGB probes - (reply: 2)
1228. PCR product dimer issue - (reply: 24)
1229. PCR additives Formamide - Formamide which one? (reply: 4)
1230. RNA from Zebrafish - (reply: 3)