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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1591. Fast and Furious: Multiplex PCR - Need expert's opinion and help---perhaps with a bit counseling (reply: 10)
1592. How to compare different expression levels between genes - (reply: 1)
1593. DNA contamination after RNA extraction, which kit works best? - (reply: 6)
1594. primer-blast from ncbi - (reply: 3)
1595. Page or HPLC primers - What is the difference (reply: 2)
1596. Standard curves for PCR efficiency. - (reply: 2)
1597. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
1598. Looking for help with my PCR! - (reply: 3)
1599. HKG validation - interplate normalisation (reply: 2)
1600. PCR without thermal cycler? - (reply: 9)
1601. primer contamination - primer contaminated with ice (reply: 3)
1602. Blue Juice - I think I need to redo... (reply: 3)
1603. Loss of volume in PCR reaction in 96 well plate - (reply: 7)
1604. RT-PCR problem - (reply: 4)
1605. home made sybr green mix for qpcr - (reply: 2)
1606. reference genes for qPCR - (reply: 3)
1607. PCR Efficiency - (reply: 2)
1608. DNA and RNA contamination in RT PCR water controls - (reply: 2)
1609. Optimal target length - (reply: 3)
1610. Fermentas SYBR Green vs. Qiagen SYBR Green - (reply: 2)
1611. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
1612. Different types/Brands of BSA? - (reply: 2)
1613. NTC with specific amplification - (reply: 1)
1614. Lost a Band by changing thermocycler - (reply: 9)
1615. Primer3 vs Primer BLAST - (reply: 3)
1616. qPCR using standard curve method - how to do correct setup? (reply: 3)
1617. Primer-Blast - (reply: 3)
1618. Amplification in NTC and noRT controls - (reply: 5)
1619. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
1620. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)