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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1591. no pcr amplification product - (reply: 9)
1592. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
1593. less steep curve and high Ct value! - (reply: 2)
1594. Question about site directed mutagenesis - (reply: 1)
1595. Amplfication of fungal DNA - (reply: 8)
1596. PCR problem - (reply: 4)
1597. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
1598. differently behavioring replicates in real-time PCR - (reply: 1)
1599. Multiplex PCR - (reply: 1)
1600. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
1601. optimal template dna concentrations - (reply: 1)
1602. reconstitution of primers - (reply: 2)
1603. Mysterious PCR Contamination - (reply: 13)
1604. RT-PCR Help - Protocol provided (reply: 4)
1605. Endogenous control in bacterial RT-qPCR - (reply: 4)
1606. Mutagenesis PCR and undesired amplification - (reply: 2)
1607. PCR-how to set 'Extension: ramping from 55 to 72 for 5min' - (reply: 4)
1608. primer dimer - (reply: 2)
1609. Genotyping conditioning-where to start? - (reply: 1)
1610. Marker migrates slower depending where is loaded - (reply: 2)
1611. PCR on degraded templates - (reply: 2)
1612. PCR help needed - (reply: 3)
1613. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
1614. Tm difference - (reply: 2)
1615. Genotyping - PCR not working (reply: 6)
1616. RT qPCR Newbie, Weird Results - (reply: 1)
1617. Low qPCR efficiency with plasmid template, why? - Low qPCR efficiency with plasmid template (reply: 5)
1618. light bands, dark smear, dark dimers - (reply: 2)
1619. PCR, followed by sequencing... - why ?? (reply: 3)
1620. qPCR and varying cDNA concentrations - (reply: 1)