Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1591. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1592. asymmetric PCR - (reply: 1)
1593. Best time to isolate RNA for qPCR? - (reply: 1)
1594. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
1595. CORRECT PCR Incorrect RTPCR - (reply: 10)
1596. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1597. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1598. Real time PCR results-interpretation - (reply: 2)
1599. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1600. Bands appearing out of no where?! - (reply: 5)
1601. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1602. SYBR melting curve vs RT-PCR gel - (reply: 1)
1603. searching housekeeping genes - (reply: 4)
1604. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1605. primer tm - tm calculation for tagged primers (reply: 2)
1606. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1607. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1608. Long real-time Amplicon - (reply: 2)
1609. PCR + phusion enzyme = massive errors - (reply: 4)
1610. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1611. RNA from MMA embedded calcified bone - (reply: 1)
1612. long DNA amplification - (reply: 11)
1613. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1614. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1615. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1616. Amplify cDNA for qRT-PCR? - (reply: 6)
1617. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1618. QPCR problem! - (reply: 9)
1619. pcr trouble solved but I cant seem to understand why - (reply: 1)
1620. Primer design if sequence is unknown for organism - (reply: 5)