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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1591. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1592. primer tm - tm calculation for tagged primers (reply: 2)
1593. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1594. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1595. Long real-time Amplicon - (reply: 2)
1596. PCR + phusion enzyme = massive errors - (reply: 4)
1597. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1598. RNA from MMA embedded calcified bone - (reply: 1)
1599. long DNA amplification - (reply: 11)
1600. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1601. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1602. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1603. Amplify cDNA for qRT-PCR? - (reply: 6)
1604. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1605. QPCR problem! - (reply: 9)
1606. pcr trouble solved but I cant seem to understand why - (reply: 1)
1607. Primer design if sequence is unknown for organism - (reply: 5)
1608. Reference gene qPCR - (reply: 4)
1609. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
1610. Real time PCR info - (reply: 4)
1611. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
1612. standard curve - (reply: 2)
1613. PCR from BAC - PCR from BAC (reply: 1)
1614. Mean versus Mediat Ct Values - Which to use for statistical analsyis (reply: 1)
1615. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
1616. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
1617. heating rna before rt reaction? - (reply: 1)
1618. relative gene expression with multiple reference genes - data analysis (reply: 1)
1619. qRT-PCR no of cycles?? - (reply: 6)
1620. Is there any setting to avoid same base runs in Primer-BLAST? - (reply: 3)