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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1591. Amplification in NTC and noRT controls - (reply: 5)
1592. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
1593. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1594. asymmetric PCR - (reply: 1)
1595. Best time to isolate RNA for qPCR? - (reply: 1)
1596. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
1597. CORRECT PCR Incorrect RTPCR - (reply: 10)
1598. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1599. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1600. Real time PCR results-interpretation - (reply: 2)
1601. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1602. Bands appearing out of no where?! - (reply: 5)
1603. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1604. SYBR melting curve vs RT-PCR gel - (reply: 1)
1605. searching housekeeping genes - (reply: 4)
1606. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1607. primer tm - tm calculation for tagged primers (reply: 2)
1608. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1609. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1610. Long real-time Amplicon - (reply: 2)
1611. PCR + phusion enzyme = massive errors - (reply: 4)
1612. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1613. RNA from MMA embedded calcified bone - (reply: 1)
1614. long DNA amplification - (reply: 11)
1615. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1616. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1617. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1618. Amplify cDNA for qRT-PCR? - (reply: 6)
1619. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1620. QPCR problem! - (reply: 9)