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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1591. DNA contamination after RNA extraction, which kit works best? - (reply: 6)
1592. primer-blast from ncbi - (reply: 3)
1593. Page or HPLC primers - What is the difference (reply: 2)
1594. Standard curves for PCR efficiency. - (reply: 2)
1595. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
1596. Looking for help with my PCR! - (reply: 3)
1597. HKG validation - interplate normalisation (reply: 2)
1598. PCR without thermal cycler? - (reply: 9)
1599. primer contamination - primer contaminated with ice (reply: 3)
1600. Blue Juice - I think I need to redo... (reply: 3)
1601. Loss of volume in PCR reaction in 96 well plate - (reply: 7)
1602. RT-PCR problem - (reply: 4)
1603. home made sybr green mix for qpcr - (reply: 2)
1604. reference genes for qPCR - (reply: 3)
1605. PCR Efficiency - (reply: 2)
1606. DNA and RNA contamination in RT PCR water controls - (reply: 2)
1607. Optimal target length - (reply: 3)
1608. Fermentas SYBR Green vs. Qiagen SYBR Green - (reply: 2)
1609. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
1610. Different types/Brands of BSA? - (reply: 2)
1611. NTC with specific amplification - (reply: 1)
1612. Lost a Band by changing thermocycler - (reply: 9)
1613. Primer3 vs Primer BLAST - (reply: 3)
1614. qPCR using standard curve method - how to do correct setup? (reply: 3)
1615. Primer-Blast - (reply: 3)
1616. Amplification in NTC and noRT controls - (reply: 5)
1617. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
1618. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1619. asymmetric PCR - (reply: 1)
1620. Best time to isolate RNA for qPCR? - (reply: 1)