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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1591. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1592. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1593. Real time PCR results-interpretation - (reply: 2)
1594. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1595. Bands appearing out of no where?! - (reply: 5)
1596. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1597. SYBR melting curve vs RT-PCR gel - (reply: 1)
1598. searching housekeeping genes - (reply: 4)
1599. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1600. primer tm - tm calculation for tagged primers (reply: 2)
1601. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1602. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1603. Long real-time Amplicon - (reply: 2)
1604. PCR + phusion enzyme = massive errors - (reply: 4)
1605. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1606. RNA from MMA embedded calcified bone - (reply: 1)
1607. long DNA amplification - (reply: 11)
1608. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1609. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1610. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1611. Amplify cDNA for qRT-PCR? - (reply: 6)
1612. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1613. QPCR problem! - (reply: 9)
1614. pcr trouble solved but I cant seem to understand why - (reply: 1)
1615. Primer design if sequence is unknown for organism - (reply: 5)
1616. Reference gene qPCR - (reply: 4)
1617. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
1618. Real time PCR info - (reply: 4)
1619. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
1620. standard curve - (reply: 2)