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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1591. Primer-Blast - (reply: 3)
1592. Amplification in NTC and noRT controls - (reply: 5)
1593. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
1594. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
1595. asymmetric PCR - (reply: 1)
1596. Best time to isolate RNA for qPCR? - (reply: 1)
1597. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
1598. CORRECT PCR Incorrect RTPCR - (reply: 10)
1599. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
1600. Confusing bands from PCR - not primer dimer, not product (reply: 30)
1601. Real time PCR results-interpretation - (reply: 2)
1602. Opinion on Eppendorf Realplex qPCR machin - (reply: 2)
1603. Bands appearing out of no where?! - (reply: 5)
1604. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1605. SYBR melting curve vs RT-PCR gel - (reply: 1)
1606. searching housekeeping genes - (reply: 4)
1607. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1608. primer tm - tm calculation for tagged primers (reply: 2)
1609. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1610. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1611. Long real-time Amplicon - (reply: 2)
1612. PCR + phusion enzyme = massive errors - (reply: 4)
1613. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1614. RNA from MMA embedded calcified bone - (reply: 1)
1615. long DNA amplification - (reply: 11)
1616. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1617. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1618. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1619. Amplify cDNA for qRT-PCR? - (reply: 6)
1620. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)