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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1591. Standard curve for RT-PCR - (reply: 1)
1592. Problem amplifying plasmid - (reply: 1)
1593. What Factors Affect Ct values of a gene? - (reply: 1)
1594. Sybr Green I in real-time PCR reaction - (reply: 3)
1595. Primers annealing temperatures - (reply: 8)
1596. Difficulties cloning by PCR - (reply: 5)
1597. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
1598. rna storage at -20 - (reply: 2)
1599. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
1600. high Cp value PCR - (reply: 2)
1601. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
1602. Synthesize own Oligo-dT primers - (reply: 6)
1603. RNA extraction for qPCR: Trizol vs. column? - (reply: 5)
1604. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
1605. dNTP or Deoxynucleotide solution Mix - (reply: 1)
1606. Smearing/ no amplification in PCR - (reply: 3)
1607. qRTPCR (TaqMan) Data Representation - (reply: 1)
1608. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
1609. Lucicferase activity and endogenous transcription - (reply: 7)
1610. need help on primer calcuation soon - (reply: 1)
1611. Heart ache in real time - (reply: 1)
1612. HEX vs. Cy-5 - (reply: 3)
1613. Direct PCR (PCR without DNA extraction) - (reply: 2)
1614. PCR using xt5 - (reply: 2)
1615. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
1616. Problems with qRT-PCR - Lots of unspecific products (reply: 2)
1617. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
1618. 2 bands or smear? 2-step RT-PCR - (reply: 1)
1619. 23S rRNA Primer - need primer sequence (reply: 1)
1620. Lowered extension temps with Phusion - (reply: 7)