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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1591. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
1592. SYBR melting curve vs RT-PCR gel - (reply: 1)
1593. searching housekeeping genes - (reply: 4)
1594. 2^-ddCT method for multiple genes - 2^-ddCT method for multiple genes (reply: 4)
1595. primer tm - tm calculation for tagged primers (reply: 2)
1596. xChIP qPCR calculation if NoAb ctrl is N.A - (reply: 1)
1597. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
1598. Long real-time Amplicon - (reply: 2)
1599. PCR + phusion enzyme = massive errors - (reply: 4)
1600. help needed in cDNA synthesis - needed help desperately,tq (reply: 3)
1601. RNA from MMA embedded calcified bone - (reply: 1)
1602. long DNA amplification - (reply: 11)
1603. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
1604. gDNA pcr product as standard for absolute quantification? - (reply: 1)
1605. qPCR - no signal detected but plenty of product on gel - (reply: 3)
1606. Amplify cDNA for qRT-PCR? - (reply: 6)
1607. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
1608. QPCR problem! - (reply: 9)
1609. pcr trouble solved but I cant seem to understand why - (reply: 1)
1610. Primer design if sequence is unknown for organism - (reply: 5)
1611. Reference gene qPCR - (reply: 4)
1612. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
1613. Real time PCR info - (reply: 4)
1614. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
1615. standard curve - (reply: 2)
1616. PCR from BAC - PCR from BAC (reply: 1)
1617. Mean versus Mediat Ct Values - Which to use for statistical analsyis (reply: 1)
1618. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
1619. High Molecular weight artifact LA-PCR 2-step - Artifact or real band - higher than expected Mw amplified product (reply: 3)
1620. heating rna before rt reaction? - (reply: 1)