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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. HRM instrumentaion - (reply: 4)
182. Can you repeat a thermocycle? - (reply: 3)
183. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
184. Amplification curve in the negative control samples - (reply: 7)
185. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
186. Primer as limiting reagent in PCR reaction - (reply: 2)
187. Taqman probe in a LightCycler 480? - (reply: 1)
188. Real time PCR for degraded RNA - (reply: 1)
189. Ct value of housekeeping gene - (reply: 4)
190. Efficiency correct fold change -- use all or none? - (reply: 1)
191. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
192. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
193. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
194. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
195. Is this primer-dimer peak in my melting curve? - (reply: 6)
196. Taq / Phusion mix - (reply: 3)
197. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
198. Dilution series between HKG and GOI - (reply: 7)
199. Reference gene(s) selection and validaton for qPCR - (reply: 5)
200. Is that normal the negative control showing signals? - (reply: 13)
201. qPCR cannot detect reaction. - (reply: 1)
202. website for primer design - (reply: 1)
203. PCR Efficiency over 150%! - (reply: 1)
204. problem of amplification - (reply: 2)
205. Rxn not working - amplify/linearize plasmid - (reply: 3)
206. qRT-PCR works one day, and not the next - (reply: 1)
207. PCR not working - (reply: 11)
208. an urgent problem with replicates - (reply: 10)
209. Site-directed mutagenesis: maximum substitution - (reply: 1)
210. Inter run calibration qRT-PCR - (reply: 2)