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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. website for primer design - (reply: 1)
182. PCR Efficiency over 150%! - (reply: 1)
183. problem of amplification - (reply: 2)
184. Rxn not working - amplify/linearize plasmid - (reply: 3)
185. qRT-PCR works one day, and not the next - (reply: 1)
186. PCR not working - (reply: 11)
187. an urgent problem with replicates - (reply: 10)
188. Site-directed mutagenesis: maximum substitution - (reply: 1)
189. Inter run calibration qRT-PCR - (reply: 2)
190. Primer design and alternative transcripts - (reply: 2)
191. qPCR amplification - (reply: 4)
192. Extremely desperate noob question: How do these PCR work? - (reply: 6)
193. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
194. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
195. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
196. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
197. Genomic DNA Isolation from Soft Agar Colonies - (reply: 3)
198. help with total RNA isolation - (reply: 1)
199. Need help to identify bands in AGE result - (reply: 3)
200. COLD-PCR (Dissociation Curve) via qPCR - (reply: 4)
201. how to hasten real-time PCR amplifications - (reply: 2)
202. Please please help me with my Phusion PCR. - (reply: 5)
203. Nanodrop vs Picogreen to quantify post-PCR product - (reply: 3)
204. Need no RT control for all genes? - (reply: 1)
205. Different annealing temp between HKG and target gene - (reply: 2)
206. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
207. help in long pcr - (reply: 1)
208. Reselling a supermix/enzyme in a commercial diagnostic kit - (reply: 2)
209. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
210. PCR inhibitor in template DNA - (reply: 3)