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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. Queries regarding PCR - (reply: 3)
182. Identification of Listeria monocytogenes - (reply: 1)
183. Calculation of Taq Polymerase - (reply: 3)
184. Best Taq for colony PCR? - (reply: 5)
185. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
186. How to test my milleq water in q PCR about contaminants - (reply: 3)
187. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
188. real time PCR primer design - (reply: 1)
189. Plasmid Linearization Problems - (reply: 9)
190. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
191. Increasing RNA yield from very small tissues - (reply: 3)
192. Degenerated Primers and their problems - (reply: 1)
193. Interpreting primer BLAST scores for self-complementary - (reply: 5)
194. Testing primers on 'unknown' tissue - (reply: 1)
195. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
196. nested PCR for low viral load- HBV patient sample - (reply: 2)
197. Finding an answer as to why I have Tm but no Ct - (reply: 6)
198. Amplification in HK gene but not for target gene - (reply: 3)
199. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
200. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
201. how to make this reaction? - (reply: 1)
202. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
203. How long can I store at - 30C my PCR mix? - (reply: 6)
204. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
205. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
206. RT-PCR help - (reply: 2)
207. Strange signals in the NTC - (reply: 1)
208. False positive - help - (reply: 3)
209. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
210. PCR Temperature change control malfunctioning - (reply: 1)