Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. Testing primers on 'unknown' tissue - (reply: 1)
182. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
183. nested PCR for low viral load- HBV patient sample - (reply: 2)
184. Finding an answer as to why I have Tm but no Ct - (reply: 6)
185. Amplification in HK gene but not for target gene - (reply: 3)
186. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
187. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
188. how to make this reaction? - (reply: 1)
189. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
190. How long can I store at - 30C my PCR mix? - (reply: 6)
191. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
192. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
193. RT-PCR help - (reply: 2)
194. Strange signals in the NTC - (reply: 1)
195. False positive - help - (reply: 3)
196. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
197. PCR Temperature change control malfunctioning - (reply: 1)
198. Assay question - (reply: 1)
199. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
200. HRM instrumentaion - (reply: 4)
201. Can you repeat a thermocycle? - (reply: 3)
202. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
203. Amplification curve in the negative control samples - (reply: 7)
204. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
205. Primer as limiting reagent in PCR reaction - (reply: 2)
206. Taqman probe in a LightCycler 480? - (reply: 1)
207. Real time PCR for degraded RNA - (reply: 1)
208. Ct value of housekeeping gene - (reply: 4)
209. Efficiency correct fold change -- use all or none? - (reply: 1)
210. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)