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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
182. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
183. RT-PCR help - (reply: 2)
184. Strange signals in the NTC - (reply: 1)
185. False positive - help - (reply: 3)
186. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
187. PCR Temperature change control malfunctioning - (reply: 1)
188. Assay question - (reply: 1)
189. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
190. HRM instrumentaion - (reply: 4)
191. Can you repeat a thermocycle? - (reply: 3)
192. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
193. Amplification curve in the negative control samples - (reply: 7)
194. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
195. Primer as limiting reagent in PCR reaction - (reply: 2)
196. Taqman probe in a LightCycler 480? - (reply: 1)
197. Real time PCR for degraded RNA - (reply: 1)
198. Ct value of housekeeping gene - (reply: 4)
199. Efficiency correct fold change -- use all or none? - (reply: 1)
200. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
201. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
202. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
203. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
204. Is this primer-dimer peak in my melting curve? - (reply: 6)
205. Taq / Phusion mix - (reply: 3)
206. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
207. Dilution series between HKG and GOI - (reply: 7)
208. Reference gene(s) selection and validaton for qPCR - (reply: 5)
209. Is that normal the negative control showing signals? - (reply: 13)
210. qPCR cannot detect reaction. - (reply: 1)