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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
182. Dilution series between HKG and GOI - (reply: 7)
183. Reference gene(s) selection and validaton for qPCR - (reply: 5)
184. Is that normal the negative control showing signals? - (reply: 13)
185. qPCR cannot detect reaction. - (reply: 1)
186. website for primer design - (reply: 1)
187. PCR Efficiency over 150%! - (reply: 1)
188. problem of amplification - (reply: 2)
189. Rxn not working - amplify/linearize plasmid - (reply: 3)
190. qRT-PCR works one day, and not the next - (reply: 1)
191. PCR not working - (reply: 11)
192. an urgent problem with replicates - (reply: 10)
193. Site-directed mutagenesis: maximum substitution - (reply: 1)
194. Inter run calibration qRT-PCR - (reply: 2)
195. Primer design and alternative transcripts - (reply: 2)
196. qPCR amplification - (reply: 4)
197. Extremely desperate noob question: How do these PCR work? - (reply: 6)
198. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
199. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
200. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
201. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
202. Genomic DNA Isolation from Soft Agar Colonies - (reply: 3)
203. help with total RNA isolation - (reply: 1)
204. Need help to identify bands in AGE result - (reply: 3)
205. COLD-PCR (Dissociation Curve) via qPCR - (reply: 4)
206. how to hasten real-time PCR amplifications - (reply: 2)
207. Please please help me with my Phusion PCR. - (reply: 5)
208. Nanodrop vs Picogreen to quantify post-PCR product - (reply: 3)
209. Need no RT control for all genes? - (reply: 1)
210. Different annealing temp between HKG and target gene - (reply: 2)