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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. Taqman probe in a LightCycler 480? - (reply: 1)
182. Real time PCR for degraded RNA - (reply: 1)
183. Ct value of housekeeping gene - (reply: 4)
184. Efficiency correct fold change -- use all or none? - (reply: 1)
185. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
186. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
187. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
188. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
189. Is this primer-dimer peak in my melting curve? - (reply: 6)
190. Taq / Phusion mix - (reply: 3)
191. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
192. Dilution series between HKG and GOI - (reply: 7)
193. Reference gene(s) selection and validaton for qPCR - (reply: 5)
194. Is that normal the negative control showing signals? - (reply: 13)
195. qPCR cannot detect reaction. - (reply: 1)
196. website for primer design - (reply: 1)
197. PCR Efficiency over 150%! - (reply: 1)
198. problem of amplification - (reply: 2)
199. Rxn not working - amplify/linearize plasmid - (reply: 3)
200. qRT-PCR works one day, and not the next - (reply: 1)
201. PCR not working - (reply: 11)
202. an urgent problem with replicates - (reply: 10)
203. Site-directed mutagenesis: maximum substitution - (reply: 1)
204. Inter run calibration qRT-PCR - (reply: 2)
205. Primer design and alternative transcripts - (reply: 2)
206. qPCR amplification - (reply: 4)
207. Extremely desperate noob question: How do these PCR work? - (reply: 6)
208. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
209. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
210. RT-PCR - High Ct Values and Laser Capture - (reply: 1)