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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. real time PCR primer design - (reply: 1)
182. Plasmid Linearization Problems - (reply: 9)
183. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
184. Increasing RNA yield from very small tissues - (reply: 3)
185. Degenerated Primers and their problems - (reply: 1)
186. Interpreting primer BLAST scores for self-complementary - (reply: 5)
187. Testing primers on 'unknown' tissue - (reply: 1)
188. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
189. nested PCR for low viral load- HBV patient sample - (reply: 2)
190. Finding an answer as to why I have Tm but no Ct - (reply: 6)
191. Amplification in HK gene but not for target gene - (reply: 3)
192. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
193. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
194. how to make this reaction? - (reply: 1)
195. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
196. How long can I store at - 30C my PCR mix? - (reply: 6)
197. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
198. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
199. RT-PCR help - (reply: 2)
200. Strange signals in the NTC - (reply: 1)
201. False positive - help - (reply: 3)
202. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
203. PCR Temperature change control malfunctioning - (reply: 1)
204. Assay question - (reply: 1)
205. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
206. HRM instrumentaion - (reply: 4)
207. Can you repeat a thermocycle? - (reply: 3)
208. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
209. Amplification curve in the negative control samples - (reply: 7)
210. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)