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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. Amplification curve in the negative control samples - (reply: 7)
182. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
183. Primer as limiting reagent in PCR reaction - (reply: 2)
184. Taqman probe in a LightCycler 480? - (reply: 1)
185. Real time PCR for degraded RNA - (reply: 1)
186. Ct value of housekeeping gene - (reply: 4)
187. Efficiency correct fold change -- use all or none? - (reply: 1)
188. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
189. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
190. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
191. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
192. Is this primer-dimer peak in my melting curve? - (reply: 6)
193. Taq / Phusion mix - (reply: 3)
194. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
195. Dilution series between HKG and GOI - (reply: 7)
196. Reference gene(s) selection and validaton for qPCR - (reply: 5)
197. Is that normal the negative control showing signals? - (reply: 13)
198. qPCR cannot detect reaction. - (reply: 1)
199. website for primer design - (reply: 1)
200. PCR Efficiency over 150%! - (reply: 1)
201. problem of amplification - (reply: 2)
202. Rxn not working - amplify/linearize plasmid - (reply: 3)
203. qRT-PCR works one day, and not the next - (reply: 1)
204. PCR not working - (reply: 11)
205. an urgent problem with replicates - (reply: 10)
206. Site-directed mutagenesis: maximum substitution - (reply: 1)
207. Inter run calibration qRT-PCR - (reply: 2)
208. Primer design and alternative transcripts - (reply: 2)
209. qPCR amplification - (reply: 4)
210. Extremely desperate noob question: How do these PCR work? - (reply: 6)