Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
182. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
183. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
184. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
185. Is this primer-dimer peak in my melting curve? - (reply: 6)
186. Taq / Phusion mix - (reply: 3)
187. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
188. Dilution series between HKG and GOI - (reply: 7)
189. Reference gene(s) selection and validaton for qPCR - (reply: 5)
190. Is that normal the negative control showing signals? - (reply: 13)
191. qPCR cannot detect reaction. - (reply: 1)
192. website for primer design - (reply: 1)
193. PCR Efficiency over 150%! - (reply: 1)
194. problem of amplification - (reply: 2)
195. Rxn not working - amplify/linearize plasmid - (reply: 3)
196. qRT-PCR works one day, and not the next - (reply: 1)
197. PCR not working - (reply: 11)
198. an urgent problem with replicates - (reply: 10)
199. Site-directed mutagenesis: maximum substitution - (reply: 1)
200. Inter run calibration qRT-PCR - (reply: 2)
201. Primer design and alternative transcripts - (reply: 2)
202. qPCR amplification - (reply: 4)
203. Extremely desperate noob question: How do these PCR work? - (reply: 6)
204. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
205. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
206. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
207. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
208. Genomic DNA Isolation from Soft Agar Colonies - (reply: 3)
209. help with total RNA isolation - (reply: 1)
210. Need help to identify bands in AGE result - (reply: 3)