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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. Poor efficiency standard curve - (reply: 2)
182. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
183. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
184. PCR gene specific amplification problem - (reply: 3)
185. Failure SYBRGREEN PCR - (reply: 4)
186. Pfaffl Method for relative - (reply: 4)
187. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
188. experiences with NuPCR from Illumina? - (reply: 1)
189. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
190. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
191. 100 ng RNA for cDNA synthesis - (reply: 4)
192. Inexplicable qPCR failure in single wells - (reply: 8)
193. negative control well 300 bp band - (reply: 3)
194. Reference gene for normalisation - for different growth rates - (reply: 3)
195. Strange amplification plots with high Ct variability - (reply: 1)
196. How big a role does mixing play in PCR - (reply: 1)
197. Melting curve is irregular for primer optimization - (reply: 5)
198. Designing primers for ABO blood groups - (reply: 1)
199. Methylight Results Analysis - (reply: 1)
200. GAPDH Ct value - (reply: 4)
201. dissociation curves - (reply: 1)
202. RT-PCR primer design - (reply: 7)
203. How to amplify very short PCR template - (reply: 4)
204. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
205. Is this primer okay? - (reply: 4)
206. Dilution calculation - (reply: 3)
207. Common causes for low RNA A260/230 ratios - (reply: 7)
208. Limit of detection for salmon gDNA? - (reply: 7)
209. Intensifying signal from positive control - (reply: 5)
210. How to avoid nonspecific amplification (band) in PCR - (reply: 5)