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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. qPCR result of pooled samples - (reply: 1)
182. transcript variant X - (reply: 5)
183. RNA extraction for RT-PCR - (reply: 3)
184. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
185. problems with gDNA doing real time PCR in yeast - (reply: 2)
186. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
187. hybridization probe - (reply: 1)
188. How to get rid of bands in PCR negative control - (reply: 10)
189. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
190. standard curve using genomic dna - (reply: 1)
191. PCR problem - (reply: 2)
192. defining ratio of alternative spliced gene with qPCR - (reply: 14)
193. Poor efficiency standard curve - (reply: 2)
194. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
195. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
196. PCR gene specific amplification problem - (reply: 3)
197. Failure SYBRGREEN PCR - (reply: 4)
198. Pfaffl Method for relative - (reply: 4)
199. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
200. experiences with NuPCR from Illumina? - (reply: 1)
201. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
202. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
203. 100 ng RNA for cDNA synthesis - (reply: 4)
204. Inexplicable qPCR failure in single wells - (reply: 10)
205. negative control well 300 bp band - (reply: 3)
206. Reference gene for normalisation - for different growth rates - (reply: 3)
207. Strange amplification plots with high Ct variability - (reply: 1)
208. How big a role does mixing play in PCR - (reply: 1)
209. Melting curve is irregular for primer optimization - (reply: 5)
210. Designing primers for ABO blood groups - (reply: 1)