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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
182. standard curve using genomic dna - (reply: 1)
183. PCR problem - (reply: 2)
184. defining ratio of alternative spliced gene with qPCR - (reply: 14)
185. Poor efficiency standard curve - (reply: 2)
186. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
187. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
188. PCR gene specific amplification problem - (reply: 3)
189. Failure SYBRGREEN PCR - (reply: 4)
190. Pfaffl Method for relative - (reply: 4)
191. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
192. experiences with NuPCR from Illumina? - (reply: 1)
193. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
194. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
195. 100 ng RNA for cDNA synthesis - (reply: 4)
196. Inexplicable qPCR failure in single wells - (reply: 10)
197. negative control well 300 bp band - (reply: 3)
198. Reference gene for normalisation - for different growth rates - (reply: 3)
199. Strange amplification plots with high Ct variability - (reply: 1)
200. How big a role does mixing play in PCR - (reply: 1)
201. Melting curve is irregular for primer optimization - (reply: 5)
202. Designing primers for ABO blood groups - (reply: 1)
203. Methylight Results Analysis - (reply: 1)
204. GAPDH Ct value - (reply: 4)
205. dissociation curves - (reply: 1)
206. RT-PCR primer design - (reply: 7)
207. How to amplify very short PCR template - (reply: 4)
208. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
209. Is this primer okay? - (reply: 4)
210. Dilution calculation - (reply: 3)