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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
182. Amplification curve in the negative control samples - (reply: 7)
183. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
184. Primer as limiting reagent in PCR reaction - (reply: 2)
185. Taqman probe in a LightCycler 480? - (reply: 1)
186. Real time PCR for degraded RNA - (reply: 1)
187. Ct value of housekeeping gene - (reply: 4)
188. Efficiency correct fold change -- use all or none? - (reply: 1)
189. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
190. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
191. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
192. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
193. Is this primer-dimer peak in my melting curve? - (reply: 6)
194. Taq / Phusion mix - (reply: 3)
195. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
196. Dilution series between HKG and GOI - (reply: 7)
197. Reference gene(s) selection and validaton for qPCR - (reply: 5)
198. Is that normal the negative control showing signals? - (reply: 13)
199. qPCR cannot detect reaction. - (reply: 1)
200. website for primer design - (reply: 1)
201. PCR Efficiency over 150%! - (reply: 1)
202. problem of amplification - (reply: 2)
203. Rxn not working - amplify/linearize plasmid - (reply: 3)
204. qRT-PCR works one day, and not the next - (reply: 1)
205. PCR not working - (reply: 11)
206. an urgent problem with replicates - (reply: 10)
207. Site-directed mutagenesis: maximum substitution - (reply: 1)
208. Inter run calibration qRT-PCR - (reply: 2)
209. Primer design and alternative transcripts - (reply: 2)
210. qPCR amplification - (reply: 4)