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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. Ct value of housekeeping gene - (reply: 4)
182. Efficiency correct fold change -- use all or none? - (reply: 1)
183. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
184. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
185. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
186. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
187. Is this primer-dimer peak in my melting curve? - (reply: 6)
188. Taq / Phusion mix - (reply: 3)
189. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
190. Dilution series between HKG and GOI - (reply: 7)
191. Reference gene(s) selection and validaton for qPCR - (reply: 5)
192. Is that normal the negative control showing signals? - (reply: 13)
193. qPCR cannot detect reaction. - (reply: 1)
194. website for primer design - (reply: 1)
195. PCR Efficiency over 150%! - (reply: 1)
196. problem of amplification - (reply: 2)
197. Rxn not working - amplify/linearize plasmid - (reply: 3)
198. qRT-PCR works one day, and not the next - (reply: 1)
199. PCR not working - (reply: 11)
200. an urgent problem with replicates - (reply: 10)
201. Site-directed mutagenesis: maximum substitution - (reply: 1)
202. Inter run calibration qRT-PCR - (reply: 2)
203. Primer design and alternative transcripts - (reply: 2)
204. qPCR amplification - (reply: 4)
205. Extremely desperate noob question: How do these PCR work? - (reply: 6)
206. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
207. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
208. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
209. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
210. Genomic DNA Isolation from Soft Agar Colonies - (reply: 3)