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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
181. RT-PCR help - (reply: 2)
182. Strange signals in the NTC - (reply: 1)
183. False positive - help - (reply: 3)
184. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
185. PCR Temperature change control malfunctioning - (reply: 1)
186. Assay question - (reply: 1)
187. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
188. HRM instrumentaion - (reply: 4)
189. Can you repeat a thermocycle? - (reply: 3)
190. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
191. Amplification curve in the negative control samples - (reply: 7)
192. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
193. Primer as limiting reagent in PCR reaction - (reply: 2)
194. Taqman probe in a LightCycler 480? - (reply: 1)
195. Real time PCR for degraded RNA - (reply: 1)
196. Ct value of housekeeping gene - (reply: 4)
197. Efficiency correct fold change -- use all or none? - (reply: 1)
198. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
199. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
200. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
201. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
202. Is this primer-dimer peak in my melting curve? - (reply: 6)
203. Taq / Phusion mix - (reply: 3)
204. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
205. Dilution series between HKG and GOI - (reply: 7)
206. Reference gene(s) selection and validaton for qPCR - (reply: 5)
207. Is that normal the negative control showing signals? - (reply: 13)
208. qPCR cannot detect reaction. - (reply: 1)
209. website for primer design - (reply: 1)
210. PCR Efficiency over 150%! - (reply: 1)