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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. PCR gene specific amplification problem - (reply: 3)
302. Failure SYBRGREEN PCR - (reply: 4)
303. Pfaffl Method for relative - (reply: 4)
304. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
305. experiences with NuPCR from Illumina? - (reply: 1)
306. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
307. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
308. 100 ng RNA for cDNA synthesis - (reply: 4)
309. Inexplicable qPCR failure in single wells - (reply: 12)
310. negative control well 300 bp band - (reply: 3)
311. Reference gene for normalisation - for different growth rates - (reply: 3)
312. Strange amplification plots with high Ct variability - (reply: 1)
313. How big a role does mixing play in PCR - (reply: 1)
314. Melting curve is irregular for primer optimization - (reply: 5)
315. Designing primers for ABO blood groups - (reply: 1)
316. Methylight Results Analysis - (reply: 1)
317. GAPDH Ct value - (reply: 4)
318. dissociation curves - (reply: 1)
319. RT-PCR primer design - (reply: 7)
320. How to amplify very short PCR template - (reply: 4)
321. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
322. Is this primer okay? - (reply: 4)
323. Dilution calculation - (reply: 3)
324. Common causes for low RNA A260/230 ratios - (reply: 7)
325. Limit of detection for salmon gDNA? - (reply: 7)
326. Intensifying signal from positive control - (reply: 5)
327. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
328. analysing qpcr data and plotting standard curve - (reply: 1)
329. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
330. PCR product as standard curve template - (reply: 6)