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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. qPCR result of pooled samples - (reply: 1)
302. transcript variant X - (reply: 5)
303. RNA extraction for RT-PCR - (reply: 3)
304. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
305. problems with gDNA doing real time PCR in yeast - (reply: 2)
306. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
307. hybridization probe - (reply: 1)
308. How to get rid of bands in PCR negative control - (reply: 10)
309. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
310. standard curve using genomic dna - (reply: 1)
311. PCR problem - (reply: 2)
312. defining ratio of alternative spliced gene with qPCR - (reply: 14)
313. Poor efficiency standard curve - (reply: 2)
314. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
315. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
316. PCR gene specific amplification problem - (reply: 3)
317. Failure SYBRGREEN PCR - (reply: 4)
318. Pfaffl Method for relative - (reply: 4)
319. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
320. experiences with NuPCR from Illumina? - (reply: 1)
321. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
322. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
323. 100 ng RNA for cDNA synthesis - (reply: 4)
324. Inexplicable qPCR failure in single wells - (reply: 12)
325. negative control well 300 bp band - (reply: 3)
326. Reference gene for normalisation - for different growth rates - (reply: 3)
327. Strange amplification plots with high Ct variability - (reply: 1)
328. How big a role does mixing play in PCR - (reply: 1)
329. Melting curve is irregular for primer optimization - (reply: 5)
330. Designing primers for ABO blood groups - (reply: 1)