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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. PCR problem - (reply: 2)
302. defining ratio of alternative spliced gene with qPCR - (reply: 14)
303. Poor efficiency standard curve - (reply: 2)
304. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
305. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
306. PCR gene specific amplification problem - (reply: 3)
307. Failure SYBRGREEN PCR - (reply: 4)
308. Pfaffl Method for relative - (reply: 4)
309. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
310. experiences with NuPCR from Illumina? - (reply: 1)
311. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
312. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
313. 100 ng RNA for cDNA synthesis - (reply: 4)
314. Inexplicable qPCR failure in single wells - (reply: 12)
315. negative control well 300 bp band - (reply: 3)
316. Reference gene for normalisation - for different growth rates - (reply: 3)
317. Strange amplification plots with high Ct variability - (reply: 1)
318. How big a role does mixing play in PCR - (reply: 1)
319. Melting curve is irregular for primer optimization - (reply: 5)
320. Designing primers for ABO blood groups - (reply: 1)
321. Methylight Results Analysis - (reply: 1)
322. GAPDH Ct value - (reply: 4)
323. dissociation curves - (reply: 1)
324. RT-PCR primer design - (reply: 7)
325. How to amplify very short PCR template - (reply: 4)
326. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
327. Is this primer okay? - (reply: 4)
328. Dilution calculation - (reply: 3)
329. Common causes for low RNA A260/230 ratios - (reply: 7)
330. Limit of detection for salmon gDNA? - (reply: 7)