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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. reference gene for qPCR in E.coli - (reply: 1)
302. Problem amplifying viral gene - (reply: 5)
303. The same CT with different Quantity, why? - (reply: 8)
304. Adding tag using overlapping PCR - (reply: 2)
305. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
306. Design primer from incomplete sequence... - (reply: 2)
307. Long term storage for RNA extracted from pancreas - (reply: 3)
308. How to perform colony PCR - (reply: 1)
309. qPCR from HEK cells transfected with Plasmid - (reply: 4)
310. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
311. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
312. Negative Control Looks Like the Rest - (reply: 11)
313. Why RNase H treatment after cDNA synthesis - (reply: 2)
314. RNA isolation from Sesame seed - (reply: 3)
315. qPCR with Genomic DNA - (reply: 1)
316. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
317. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
318. Homogenize pancreas for RNA extraction - (reply: 3)
319. Point mutagenesis..... - (reply: 6)
320. No bands in PCR after DNase treatment - (reply: 4)
321. Overlap PCR problem - (reply: 5)
322. PCR product purification - (reply: 4)
323. Overlapping sequence PCR primers - (reply: 1)
324. acceptable melt curve? - (reply: 1)
325. Calibrator sample - (reply: 4)
326. Ct value very high. What can I do to improve this ct? - (reply: 4)
327. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
328. Amount of RNA (ng) for good ct values. - (reply: 3)
329. Normalizing qPCR data - (reply: 1)
330. doing PCR using PCR product , help plz !! - (reply: 5)