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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. RNA extraction for RT-PCR - (reply: 3)
302. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
303. problems with gDNA doing real time PCR in yeast - (reply: 2)
304. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
305. hybridization probe - (reply: 1)
306. How to get rid of bands in PCR negative control - (reply: 10)
307. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
308. standard curve using genomic dna - (reply: 1)
309. PCR problem - (reply: 2)
310. defining ratio of alternative spliced gene with qPCR - (reply: 14)
311. Poor efficiency standard curve - (reply: 2)
312. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
313. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
314. PCR gene specific amplification problem - (reply: 3)
315. Failure SYBRGREEN PCR - (reply: 4)
316. Pfaffl Method for relative - (reply: 4)
317. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
318. experiences with NuPCR from Illumina? - (reply: 1)
319. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
320. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
321. 100 ng RNA for cDNA synthesis - (reply: 4)
322. Inexplicable qPCR failure in single wells - (reply: 12)
323. negative control well 300 bp band - (reply: 3)
324. Reference gene for normalisation - for different growth rates - (reply: 3)
325. Strange amplification plots with high Ct variability - (reply: 1)
326. How big a role does mixing play in PCR - (reply: 1)
327. Melting curve is irregular for primer optimization - (reply: 5)
328. Designing primers for ABO blood groups - (reply: 1)
329. Methylight Results Analysis - (reply: 1)
330. GAPDH Ct value - (reply: 4)