Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. random primers or oligodT - (reply: 4)
302. Confused about normalization - (reply: 2)
303. To design or use published primers? - (reply: 4)
304. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
305. PCR primer usage (Clonning & cDNA) - (reply: 2)
306. Taq polymerase - (reply: 7)
307. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
308. Which High-Fidelity polymerase is better? - (reply: 2)
309. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
310. reference gene for qPCR in E.coli - (reply: 1)
311. Problem amplifying viral gene - (reply: 5)
312. The same CT with different Quantity, why? - (reply: 8)
313. Adding tag using overlapping PCR - (reply: 2)
314. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
315. Design primer from incomplete sequence... - (reply: 2)
316. Long term storage for RNA extracted from pancreas - (reply: 3)
317. How to perform colony PCR - (reply: 1)
318. qPCR from HEK cells transfected with Plasmid - (reply: 4)
319. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
320. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
321. Negative Control Looks Like the Rest - (reply: 11)
322. Why RNase H treatment after cDNA synthesis - (reply: 2)
323. RNA isolation from Sesame seed - (reply: 3)
324. qPCR with Genomic DNA - (reply: 1)
325. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
326. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
327. Homogenize pancreas for RNA extraction - (reply: 3)
328. Point mutagenesis..... - (reply: 6)
329. No bands in PCR after DNase treatment - (reply: 4)
330. Overlap PCR problem - (reply: 5)