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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. defining ratio of alternative spliced gene with qPCR - (reply: 14)
302. Poor efficiency standard curve - (reply: 2)
303. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
304. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
305. PCR gene specific amplification problem - (reply: 3)
306. Failure SYBRGREEN PCR - (reply: 4)
307. Pfaffl Method for relative - (reply: 4)
308. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
309. experiences with NuPCR from Illumina? - (reply: 1)
310. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
311. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
312. 100 ng RNA for cDNA synthesis - (reply: 4)
313. Inexplicable qPCR failure in single wells - (reply: 12)
314. negative control well 300 bp band - (reply: 3)
315. Reference gene for normalisation - for different growth rates - (reply: 3)
316. Strange amplification plots with high Ct variability - (reply: 1)
317. How big a role does mixing play in PCR - (reply: 1)
318. Melting curve is irregular for primer optimization - (reply: 5)
319. Designing primers for ABO blood groups - (reply: 1)
320. Methylight Results Analysis - (reply: 1)
321. GAPDH Ct value - (reply: 4)
322. dissociation curves - (reply: 1)
323. RT-PCR primer design - (reply: 7)
324. How to amplify very short PCR template - (reply: 4)
325. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
326. Is this primer okay? - (reply: 4)
327. Dilution calculation - (reply: 3)
328. Common causes for low RNA A260/230 ratios - (reply: 7)
329. Limit of detection for salmon gDNA? - (reply: 7)
330. Intensifying signal from positive control - (reply: 5)