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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. problems with gDNA doing real time PCR in yeast - (reply: 2)
302. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
303. hybridization probe - (reply: 1)
304. How to get rid of bands in PCR negative control - (reply: 10)
305. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
306. standard curve using genomic dna - (reply: 1)
307. PCR problem - (reply: 2)
308. defining ratio of alternative spliced gene with qPCR - (reply: 14)
309. Poor efficiency standard curve - (reply: 2)
310. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
311. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
312. PCR gene specific amplification problem - (reply: 3)
313. Failure SYBRGREEN PCR - (reply: 4)
314. Pfaffl Method for relative - (reply: 4)
315. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
316. experiences with NuPCR from Illumina? - (reply: 1)
317. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
318. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
319. 100 ng RNA for cDNA synthesis - (reply: 4)
320. Inexplicable qPCR failure in single wells - (reply: 12)
321. negative control well 300 bp band - (reply: 3)
322. Reference gene for normalisation - for different growth rates - (reply: 3)
323. Strange amplification plots with high Ct variability - (reply: 1)
324. How big a role does mixing play in PCR - (reply: 1)
325. Melting curve is irregular for primer optimization - (reply: 5)
326. Designing primers for ABO blood groups - (reply: 1)
327. Methylight Results Analysis - (reply: 1)
328. GAPDH Ct value - (reply: 4)
329. dissociation curves - (reply: 1)
330. RT-PCR primer design - (reply: 7)