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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. I don't find housekeeping genes: what should I do? - (reply: 6)
302. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
303. excess rna sample affect qpcr? - (reply: 2)
304. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
305. finding the corrosponding primers - (reply: 2)
306. Gene sequence for Real time PCR - (reply: 2)
307. qPCR result of pooled samples - (reply: 1)
308. transcript variant X - (reply: 5)
309. RNA extraction for RT-PCR - (reply: 3)
310. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
311. problems with gDNA doing real time PCR in yeast - (reply: 2)
312. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
313. hybridization probe - (reply: 1)
314. How to get rid of bands in PCR negative control - (reply: 10)
315. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
316. standard curve using genomic dna - (reply: 1)
317. PCR problem - (reply: 2)
318. defining ratio of alternative spliced gene with qPCR - (reply: 14)
319. Poor efficiency standard curve - (reply: 2)
320. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
321. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
322. PCR gene specific amplification problem - (reply: 3)
323. Failure SYBRGREEN PCR - (reply: 4)
324. Pfaffl Method for relative - (reply: 4)
325. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
326. experiences with NuPCR from Illumina? - (reply: 1)
327. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
328. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
329. 100 ng RNA for cDNA synthesis - (reply: 4)
330. Inexplicable qPCR failure in single wells - (reply: 12)