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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. PCR product as template for in vitro transcription - (reply: 1)
302. Sybr Green vs Taqman -- a practical approach - (reply: 2)
303. Problem with 3 step PCR - (reply: 3)
304. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
305. ARMS-PCR - (reply: 1)
306. Scaling up PCR to get more DNA - (reply: 5)
307. RNA concentration suddenly drops - (reply: 3)
308. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
309. PCR to get 10kbp product - (reply: 4)
310. site-directed mutagenesis primer Tm - (reply: 4)
311. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
312. RNA concentration to start RT reaction - (reply: 1)
313. Random vs oligo primer in preamplification RT - (reply: 1)
314. Smart Cycler II software and Absolute quantification help - (reply: 1)
315. cDNA amount in qPCR - (reply: 2)
316. mRNA integrity for qPCR - (reply: 6)
317. Strange control contamination in cDNA synthesis - (reply: 2)
318. how does polymerase stop at the required length - (reply: 1)
319. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)
320. Real-time efficiency question - (reply: 1)
321. Selective removal of standards post qPCR Run - (reply: 1)
322. Primer concentration - stupid question - (reply: 3)
323. Primer Design, help im New - (reply: 4)
324. qPCR question (no results?) - (reply: 1)
325. Artificial RNA as normalizer (HK gene) - (reply: 1)
326. Sensitivity of RT-PCR and qPCR - (reply: 4)
327. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
328. real time PCR trouble - (reply: 3)
329. removal of ethanol in PCR product purification - (reply: 5)
330. Crazy real time PCR curve - (reply: 4)