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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. qPCR - no amplification curve but suitable melting curve - (reply: 3)
302. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
303. Basic question about qPCR and endogenous RNA control. - (reply: 4)
304. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
305. something very basic, why called "event-specific detection" - (reply: 1)
306. skip 65 degrees protocol of Reverse transcription - (reply: 4)
307. Mystery in my PCR - (reply: 5)
308. Whole genome amplification from cDNA? - (reply: 4)
309. What do you use to check primer secondary structure? - (reply: 3)
310. CT of qPCR - (reply: 3)
311. Need help with my real time RT-PCR Plate Set up - (reply: 8)
312. PCR product as template for in vitro transcription - (reply: 1)
313. Sybr Green vs Taqman -- a practical approach - (reply: 2)
314. Problem with 3 step PCR - (reply: 3)
315. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
316. ARMS-PCR - (reply: 1)
317. Scaling up PCR to get more DNA - (reply: 5)
318. RNA concentration suddenly drops - (reply: 3)
319. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
320. PCR to get 10kbp product - (reply: 4)
321. site-directed mutagenesis primer Tm - (reply: 4)
322. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
323. RNA concentration to start RT reaction - (reply: 1)
324. Random vs oligo primer in preamplification RT - (reply: 1)
325. Smart Cycler II software and Absolute quantification help - (reply: 1)
326. cDNA amount in qPCR - (reply: 2)
327. mRNA integrity for qPCR - (reply: 6)
328. Strange control contamination in cDNA synthesis - (reply: 2)
329. how does polymerase stop at the required length - (reply: 1)
330. Perfect dilution curve, but double peak melting curve on SYBR qPCR - (reply: 7)