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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
302. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
303. PCR gene specific amplification problem - (reply: 3)
304. Failure SYBRGREEN PCR - (reply: 4)
305. Pfaffl Method for relative - (reply: 4)
306. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
307. experiences with NuPCR from Illumina? - (reply: 1)
308. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
309. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
310. 100 ng RNA for cDNA synthesis - (reply: 4)
311. Inexplicable qPCR failure in single wells - (reply: 12)
312. negative control well 300 bp band - (reply: 3)
313. Reference gene for normalisation - for different growth rates - (reply: 3)
314. Strange amplification plots with high Ct variability - (reply: 1)
315. How big a role does mixing play in PCR - (reply: 1)
316. Melting curve is irregular for primer optimization - (reply: 5)
317. Designing primers for ABO blood groups - (reply: 1)
318. Methylight Results Analysis - (reply: 1)
319. GAPDH Ct value - (reply: 4)
320. dissociation curves - (reply: 1)
321. RT-PCR primer design - (reply: 7)
322. How to amplify very short PCR template - (reply: 4)
323. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
324. Is this primer okay? - (reply: 4)
325. Dilution calculation - (reply: 3)
326. Common causes for low RNA A260/230 ratios - (reply: 7)
327. Limit of detection for salmon gDNA? - (reply: 7)
328. Intensifying signal from positive control - (reply: 5)
329. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
330. analysing qpcr data and plotting standard curve - (reply: 1)