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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
302. problems with gDNA doing real time PCR in yeast - (reply: 2)
303. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
304. hybridization probe - (reply: 1)
305. How to get rid of bands in PCR negative control - (reply: 10)
306. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
307. standard curve using genomic dna - (reply: 1)
308. PCR problem - (reply: 2)
309. defining ratio of alternative spliced gene with qPCR - (reply: 14)
310. Poor efficiency standard curve - (reply: 2)
311. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
312. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
313. PCR gene specific amplification problem - (reply: 3)
314. Failure SYBRGREEN PCR - (reply: 4)
315. Pfaffl Method for relative - (reply: 4)
316. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
317. experiences with NuPCR from Illumina? - (reply: 1)
318. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
319. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
320. 100 ng RNA for cDNA synthesis - (reply: 4)
321. Inexplicable qPCR failure in single wells - (reply: 12)
322. negative control well 300 bp band - (reply: 3)
323. Reference gene for normalisation - for different growth rates - (reply: 3)
324. Strange amplification plots with high Ct variability - (reply: 1)
325. How big a role does mixing play in PCR - (reply: 1)
326. Melting curve is irregular for primer optimization - (reply: 5)
327. Designing primers for ABO blood groups - (reply: 1)
328. Methylight Results Analysis - (reply: 1)
329. GAPDH Ct value - (reply: 4)
330. dissociation curves - (reply: 1)