Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
302. Wierd Bands after PCR....Confused - (reply: 9)
303. PCR with Plasmid recovered from filter paper - (reply: 6)
304. PCR amplified product size - (reply: 5)
305. Opinion: What fold change is actually considered meaningful - (reply: 2)
306. Buffers RNase decontamination - (reply: 1)
307. the storage time for primers - (reply: 9)
308. Annealing temperature for PCR - (reply: 8)
309. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
310. Nested PCR - (reply: 2)
311. random primers or oligodT - (reply: 4)
312. Confused about normalization - (reply: 2)
313. To design or use published primers? - (reply: 4)
314. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
315. PCR primer usage (Clonning & cDNA) - (reply: 2)
316. Taq polymerase - (reply: 7)
317. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
318. Which High-Fidelity polymerase is better? - (reply: 2)
319. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
320. reference gene for qPCR in E.coli - (reply: 1)
321. Problem amplifying viral gene - (reply: 5)
322. The same CT with different Quantity, why? - (reply: 8)
323. Adding tag using overlapping PCR - (reply: 2)
324. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
325. Design primer from incomplete sequence... - (reply: 2)
326. Long term storage for RNA extracted from pancreas - (reply: 3)
327. How to perform colony PCR - (reply: 1)
328. qPCR from HEK cells transfected with Plasmid - (reply: 4)
329. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
330. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)