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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
302. 100 ng RNA for cDNA synthesis - (reply: 4)
303. Inexplicable qPCR failure in single wells - (reply: 12)
304. negative control well 300 bp band - (reply: 3)
305. Reference gene for normalisation - for different growth rates - (reply: 3)
306. Strange amplification plots with high Ct variability - (reply: 1)
307. How big a role does mixing play in PCR - (reply: 1)
308. Melting curve is irregular for primer optimization - (reply: 5)
309. Designing primers for ABO blood groups - (reply: 1)
310. Methylight Results Analysis - (reply: 1)
311. GAPDH Ct value - (reply: 4)
312. dissociation curves - (reply: 1)
313. RT-PCR primer design - (reply: 7)
314. How to amplify very short PCR template - (reply: 4)
315. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
316. Is this primer okay? - (reply: 4)
317. Dilution calculation - (reply: 3)
318. Common causes for low RNA A260/230 ratios - (reply: 7)
319. Limit of detection for salmon gDNA? - (reply: 7)
320. Intensifying signal from positive control - (reply: 5)
321. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
322. analysing qpcr data and plotting standard curve - (reply: 1)
323. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
324. PCR product as standard curve template - (reply: 6)
325. TaqMan CN assay - control sample duplicated - (reply: 1)
326. qPCR - no amplification curve but suitable melting curve - (reply: 3)
327. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
328. Basic question about qPCR and endogenous RNA control. - (reply: 4)
329. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
330. something very basic, why called "event-specific detection" - (reply: 1)