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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
302. finding the corrosponding primers - (reply: 2)
303. Gene sequence for Real time PCR - (reply: 2)
304. qPCR result of pooled samples - (reply: 1)
305. transcript variant X - (reply: 5)
306. RNA extraction for RT-PCR - (reply: 3)
307. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
308. problems with gDNA doing real time PCR in yeast - (reply: 2)
309. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
310. hybridization probe - (reply: 1)
311. How to get rid of bands in PCR negative control - (reply: 10)
312. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
313. standard curve using genomic dna - (reply: 1)
314. PCR problem - (reply: 2)
315. defining ratio of alternative spliced gene with qPCR - (reply: 14)
316. Poor efficiency standard curve - (reply: 2)
317. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
318. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
319. PCR gene specific amplification problem - (reply: 3)
320. Failure SYBRGREEN PCR - (reply: 4)
321. Pfaffl Method for relative - (reply: 4)
322. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
323. experiences with NuPCR from Illumina? - (reply: 1)
324. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
325. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
326. 100 ng RNA for cDNA synthesis - (reply: 4)
327. Inexplicable qPCR failure in single wells - (reply: 12)
328. negative control well 300 bp band - (reply: 3)
329. Reference gene for normalisation - for different growth rates - (reply: 3)
330. Strange amplification plots with high Ct variability - (reply: 1)