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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. Taq polymerase - (reply: 7)
302. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
303. Which High-Fidelity polymerase is better? - (reply: 2)
304. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
305. reference gene for qPCR in E.coli - (reply: 1)
306. Problem amplifying viral gene - (reply: 5)
307. The same CT with different Quantity, why? - (reply: 8)
308. Adding tag using overlapping PCR - (reply: 2)
309. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
310. Design primer from incomplete sequence... - (reply: 2)
311. Long term storage for RNA extracted from pancreas - (reply: 3)
312. How to perform colony PCR - (reply: 1)
313. qPCR from HEK cells transfected with Plasmid - (reply: 4)
314. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
315. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
316. Negative Control Looks Like the Rest - (reply: 11)
317. Why RNase H treatment after cDNA synthesis - (reply: 2)
318. RNA isolation from Sesame seed - (reply: 3)
319. qPCR with Genomic DNA - (reply: 1)
320. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
321. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
322. Homogenize pancreas for RNA extraction - (reply: 3)
323. Point mutagenesis..... - (reply: 6)
324. No bands in PCR after DNase treatment - (reply: 4)
325. Overlap PCR problem - (reply: 5)
326. PCR product purification - (reply: 4)
327. Overlapping sequence PCR primers - (reply: 1)
328. acceptable melt curve? - (reply: 1)
329. Calibrator sample - (reply: 4)
330. Ct value very high. What can I do to improve this ct? - (reply: 4)