Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. qPCR reference gene testing help - (reply: 4)
302. protocol to relieve melanin inhibition of PCR - (reply: 4)
303. No bands despite different primers and conditions and TAqs - (reply: 8)
304. Multiplex TaqMan-like Assay PCR Efficiency - (reply: 3)
305. Wrong PCR product - (reply: 2)
306. Primer design - (reply: 5)
307. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
308. Quantitative RT-PCR statistics help - (reply: 1)
309. PCR Primer trouble - (reply: 2)
310. I don't find housekeeping genes: what should I do? - (reply: 6)
311. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
312. excess rna sample affect qpcr? - (reply: 2)
313. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
314. finding the corrosponding primers - (reply: 2)
315. Gene sequence for Real time PCR - (reply: 2)
316. qPCR result of pooled samples - (reply: 1)
317. transcript variant X - (reply: 5)
318. RNA extraction for RT-PCR - (reply: 3)
319. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
320. problems with gDNA doing real time PCR in yeast - (reply: 2)
321. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
322. hybridization probe - (reply: 1)
323. How to get rid of bands in PCR negative control - (reply: 10)
324. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
325. standard curve using genomic dna - (reply: 1)
326. PCR problem - (reply: 2)
327. defining ratio of alternative spliced gene with qPCR - (reply: 14)
328. Poor efficiency standard curve - (reply: 2)
329. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
330. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)