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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
301. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
302. standard curve using genomic dna - (reply: 1)
303. PCR problem - (reply: 2)
304. defining ratio of alternative spliced gene with qPCR - (reply: 14)
305. Poor efficiency standard curve - (reply: 2)
306. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
307. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
308. PCR gene specific amplification problem - (reply: 3)
309. Failure SYBRGREEN PCR - (reply: 4)
310. Pfaffl Method for relative - (reply: 4)
311. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
312. experiences with NuPCR from Illumina? - (reply: 1)
313. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
314. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
315. 100 ng RNA for cDNA synthesis - (reply: 4)
316. Inexplicable qPCR failure in single wells - (reply: 12)
317. negative control well 300 bp band - (reply: 3)
318. Reference gene for normalisation - for different growth rates - (reply: 3)
319. Strange amplification plots with high Ct variability - (reply: 1)
320. How big a role does mixing play in PCR - (reply: 1)
321. Melting curve is irregular for primer optimization - (reply: 5)
322. Designing primers for ABO blood groups - (reply: 1)
323. Methylight Results Analysis - (reply: 1)
324. GAPDH Ct value - (reply: 4)
325. dissociation curves - (reply: 1)
326. RT-PCR primer design - (reply: 7)
327. How to amplify very short PCR template - (reply: 4)
328. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
329. Is this primer okay? - (reply: 4)
330. Dilution calculation - (reply: 3)