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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. Finding an answer as to why I have Tm but no Ct - (reply: 6)
62. Amplification in HK gene but not for target gene - (reply: 3)
63. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
64. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
65. how to make this reaction? - (reply: 1)
66. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
67. How long can I store at - 30C my PCR mix? - (reply: 6)
68. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
69. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
70. RT-PCR help - (reply: 2)
71. Strange signals in the NTC - (reply: 1)
72. False positive - help - (reply: 3)
73. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
74. PCR Temperature change control malfunctioning - (reply: 1)
75. Assay question - (reply: 1)
76. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
77. HRM instrumentaion - (reply: 4)
78. Can you repeat a thermocycle? - (reply: 3)
79. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
80. Amplification curve in the negative control samples - (reply: 7)
81. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
82. Primer as limiting reagent in PCR reaction - (reply: 2)
83. Taqman probe in a LightCycler 480? - (reply: 1)
84. Real time PCR for degraded RNA - (reply: 1)
85. Ct value of housekeeping gene - (reply: 4)
86. Efficiency correct fold change -- use all or none? - (reply: 1)
87. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
88. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
89. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
90. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)