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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. How to make PCR analysis using the 22DDCT Method - (reply: 1)
62. qPCR data analysis from raw data - (reply: 1)
63. cDNA degradation within a week at -20? - (reply: 2)
64. Primer calculation for qPCR - final reaction - (reply: 1)
65. Sec. structure in primers - (reply: 1)
66. Differences in shape of melt curve - (reply: 4)
67. Amount of RNA for cDNA - (reply: 4)
68. Difference in melting temperature - (reply: 5)
69. Changing PCR recipe halfway - (reply: 1)
70. PCR master mix containing primers and Taq - (reply: 4)
71. Primer working with some cDNA samples and not other - (reply: 2)
72. no bands after pcr!!!! - (reply: 4)
73. Help with definitons for qPCR - (reply: 1)
74. Calibrator in qPCR - (reply: 7)
75. Experimental design for RT-PCR - (reply: 8)
76. Primer dilution - (reply: 7)
77. Calculation help - (reply: 6)
78. standards for qPCR and some other doubts - (reply: 9)
79. Plasma cortisol/corticosterone level analysis using qPCR - (reply: 1)
80. Technical Replicates vs Biological Replicates - (reply: 1)
81. pcr - (reply: 1)
82. Difference in synthesis - (reply: 1)
83. Standardizing cDNA concentration before doing PCR - (reply: 4)
84. Agarose Gel Electrophoresis - (reply: 1)
85. Volume of Primers - (reply: 4)
86. qPCR with different genes - (reply: 5)
87. PCR conditions unknown? - (reply: 10)
88. PCR anomaly in a mid-range dilution of the template - (reply: 2)
89. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
90. 2 peaks for identical sequences in HRM - (reply: 2)