Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. Standardizing cDNA concentration before doing PCR - (reply: 4)
62. Agarose Gel Electrophoresis - (reply: 1)
63. Volume of Primers - (reply: 4)
64. qPCR with different genes - (reply: 5)
65. PCR conditions unknown? - (reply: 10)
66. PCR anomaly in a mid-range dilution of the template - (reply: 2)
67. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
68. 2 peaks for identical sequences in HRM - (reply: 2)
69. PCR using very long oligos !!! - (reply: 4)
70. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
71. How to optimize a master mix kit? - (reply: 7)
72. pcr primer design - (reply: 6)
73. Please help (primer/ probe final volume calculations) - (reply: 1)
74. Primer design with EcoRI ends - (reply: 2)
75. qPCR normalization - (reply: 4)
76. Reference Gene/HKG- every plate - (reply: 2)
77. qPCR standard for lipase genes - (reply: 2)
78. PCR with overlapping primers only (primer extention) - (reply: 3)
79. Random chuck of nonsense in middle of PCR - (reply: 6)
80. Campylobacter jejuni qPCR targeting VS1 gene - (reply: 2)
81. RT-PCR analysis - (reply: 1)
82. Newbie to qPCR - (reply: 4)
83. real time qPCR standard range plasmid - (reply: 3)
84. New to PCR - (reply: 3)
85. How can I check the quality of bacterial primers and their specificity on blast? - (reply: 1)
86. Who can advise me a kit that would allow me to purify amplicons from quantitativ - (reply: 3)
87. 3-primer PCR Genotyping - problem in hemizygotes - (reply: 2)
88. t-test & qRT-PCR - (reply: 1)
89. Primer design - (reply: 1)
90. What % of colon cancer patients does a general colon PCR array detect - (reply: 1)