Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. When to dilute samples for qPCR - (reply: 2)
62. Some info. on PCR products stability w/respect to shipping - (reply: 3)
63. 3' RACE troubles - (reply: 2)
64. haeting RNA before electrophoresis - (reply: 1)
65. PCR Products not run well in the GEL - (reply: 1)
66. Qubit Nanodrop Quantifications - (reply: 1)
67. Decontaminating DNA - Effective Means? - (reply: 4)
68. Consistancy issue with PCR block - (reply: 1)
69. Queries regarding PCR - (reply: 3)
70. Identification of Listeria monocytogenes - (reply: 1)
71. Calculation of Taq Polymerase - (reply: 3)
72. Best Taq for colony PCR? - (reply: 5)
73. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
74. How to test my milleq water in q PCR about contaminants - (reply: 3)
75. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
76. real time PCR primer design - (reply: 1)
77. Plasmid Linearization Problems - (reply: 9)
78. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
79. Increasing RNA yield from very small tissues - (reply: 3)
80. Degenerated Primers and their problems - (reply: 1)
81. Interpreting primer BLAST scores for self-complementary - (reply: 5)
82. Testing primers on 'unknown' tissue - (reply: 1)
83. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
84. nested PCR for low viral load- HBV patient sample - (reply: 2)
85. Finding an answer as to why I have Tm but no Ct - (reply: 6)
86. Amplification in HK gene but not for target gene - (reply: 3)
87. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
88. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
89. how to make this reaction? - (reply: 1)
90. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)