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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. real time PCR primer design - (reply: 1)
62. Plasmid Linearization Problems - (reply: 9)
63. Plasmid calculation question (Standard Curve Quantification) - (reply: 6)
64. Increasing RNA yield from very small tissues - (reply: 3)
65. Degenerated Primers and their problems - (reply: 1)
66. Interpreting primer BLAST scores for self-complementary - (reply: 5)
67. Testing primers on 'unknown' tissue - (reply: 1)
68. Second Hand Roche Lightcycler II maching - to buy or not to buy - (reply: 1)
69. nested PCR for low viral load- HBV patient sample - (reply: 2)
70. Finding an answer as to why I have Tm but no Ct - (reply: 6)
71. Amplification in HK gene but not for target gene - (reply: 3)
72. Missing DNA Target and Effect on Non-Specific Amplicons - (reply: 1)
73. 3 RACE-PCR of cDNA ends yielded a piece of 2 different genes? - (reply: 2)
74. how to make this reaction? - (reply: 1)
75. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
76. How long can I store at - 30C my PCR mix? - (reply: 6)
77. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
78. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
79. RT-PCR help - (reply: 2)
80. Strange signals in the NTC - (reply: 1)
81. False positive - help - (reply: 3)
82. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
83. PCR Temperature change control malfunctioning - (reply: 1)
84. Assay question - (reply: 1)
85. WHY NO ONE REPLIES, is this a stupid question ? - (reply: 5)
86. HRM instrumentaion - (reply: 4)
87. Can you repeat a thermocycle? - (reply: 3)
88. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
89. Amplification curve in the negative control samples - (reply: 7)
90. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)