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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. cDNA degradation within a week at -20? - (reply: 2)
62. Primer calculation for qPCR - final reaction - (reply: 1)
63. Sec. structure in primers - (reply: 1)
64. Differences in shape of melt curve - (reply: 4)
65. Amount of RNA for cDNA - (reply: 4)
66. Difference in melting temperature - (reply: 5)
67. Changing PCR recipe halfway - (reply: 1)
68. PCR master mix containing primers and Taq - (reply: 4)
69. Primer working with some cDNA samples and not other - (reply: 2)
70. no bands after pcr!!!! - (reply: 4)
71. Help with definitons for qPCR - (reply: 1)
72. Calibrator in qPCR - (reply: 7)
73. Experimental design for RT-PCR - (reply: 8)
74. Primer dilution - (reply: 7)
75. Calculation help - (reply: 6)
76. standards for qPCR and some other doubts - (reply: 9)
77. Plasma cortisol/corticosterone level analysis using qPCR - (reply: 1)
78. Technical Replicates vs Biological Replicates - (reply: 1)
79. pcr - (reply: 1)
80. Difference in synthesis - (reply: 1)
81. Standardizing cDNA concentration before doing PCR - (reply: 4)
82. Agarose Gel Electrophoresis - (reply: 1)
83. Volume of Primers - (reply: 4)
84. qPCR with different genes - (reply: 5)
85. PCR conditions unknown? - (reply: 10)
86. PCR anomaly in a mid-range dilution of the template - (reply: 2)
87. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
88. 2 peaks for identical sequences in HRM - (reply: 2)
89. PCR using very long oligos !!! - (reply: 4)
90. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)