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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. Amount of RNA for cDNA - (reply: 4)
62. Difference in melting temperature - (reply: 5)
63. Changing PCR recipe halfway - (reply: 1)
64. PCR master mix containing primers and Taq - (reply: 4)
65. Primer working with some cDNA samples and not other - (reply: 2)
66. no bands after pcr!!!! - (reply: 4)
67. Help with definitons for qPCR - (reply: 1)
68. Calibrator in qPCR - (reply: 7)
69. Experimental design for RT-PCR - (reply: 8)
70. Primer dilution - (reply: 7)
71. Calculation help - (reply: 6)
72. standards for qPCR and some other doubts - (reply: 9)
73. Plasma cortisol/corticosterone level analysis using qPCR - (reply: 1)
74. Technical Replicates vs Biological Replicates - (reply: 1)
75. pcr - (reply: 1)
76. Difference in synthesis - (reply: 1)
77. Standardizing cDNA concentration before doing PCR - (reply: 4)
78. Agarose Gel Electrophoresis - (reply: 1)
79. Volume of Primers - (reply: 4)
80. qPCR with different genes - (reply: 5)
81. PCR conditions unknown? - (reply: 10)
82. PCR anomaly in a mid-range dilution of the template - (reply: 2)
83. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
84. 2 peaks for identical sequences in HRM - (reply: 2)
85. PCR using very long oligos !!! - (reply: 4)
86. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
87. How to optimize a master mix kit? - (reply: 7)
88. pcr primer design - (reply: 6)
89. Please help (primer/ probe final volume calculations) - (reply: 1)
90. Primer design with EcoRI ends - (reply: 2)