Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. Reference gene(s) selection and validaton for qPCR - (reply: 2)
62. Is that normal the negative control showing signals? - (reply: 13)
63. qPCR cannot detect reaction. - (reply: 1)
64. website for primer design - (reply: 1)
65. PCR Efficiency over 150%! - (reply: 1)
66. problem of amplification - (reply: 2)
67. Rxn not working - amplify/linearize plasmid - (reply: 3)
68. qRT-PCR works one day, and not the next - (reply: 1)
69. PCR not working - (reply: 11)
70. an urgent problem with replicates - (reply: 10)
71. Site-directed mutagenesis: maximum substitution - (reply: 1)
72. Inter run calibration qRT-PCR - (reply: 2)
73. Primer design and alternative transcripts - (reply: 2)
74. qPCR amplification - (reply: 4)
75. Extremely desperate noob question: How do these PCR work? - (reply: 6)
76. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
77. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
78. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
79. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
80. Genomic DNA Isolation from Soft Agar Colonies - (reply: 3)
81. help with total RNA isolation - (reply: 1)
82. Need help to identify bands in AGE result - (reply: 3)
83. COLD-PCR (Dissociation Curve) via qPCR - (reply: 4)
84. how to hasten real-time PCR amplifications - (reply: 2)
85. Please please help me with my Phusion PCR. - (reply: 5)
86. Nanodrop vs Picogreen to quantify post-PCR product - (reply: 3)
87. Need no RT control for all genes? - (reply: 1)
88. Different annealing temp between HKG and target gene - (reply: 2)
89. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
90. help in long pcr - (reply: 1)