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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. Addition of DNase after RNA extraction - (reply: 4)
62. RT-PCR electrophorasis - (reply: 1)
63. PCR contamination - (reply: 6)
64. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
65. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
66. High background fluorescence detected before starting PCR - (reply: 3)
67. Real-time pcr - (reply: 1)
68. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
69. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
70. Combining standard error of normalised fold change values - Qbase method - (reply: 2)
71. What cycle is best for analysing data? - (reply: 2)
72. Mineral oil quality in PCR tubes - (reply: 5)
73. Internal control for absolute quantification - (reply: 3)
74. Diagnostic PCR troubleshooting - (reply: 2)
75. QPCR replicates issue - (reply: 1)
76. RT-PCR Kit's Validation - (reply: 1)
77. Rt pcr - (reply: 4)
78. Viral Quantification - (reply: 1)
79. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
80. Housekeeping genes - Cq values - (reply: 4)
81. typical mRNA test with huge fold change? Any suggestions - (reply: 1)
82. PCR Amplification Issues and Primer dimer - (reply: 4)
83. Two consecutive ATG - (reply: 2)
84. How to make PCR analysis using the 22DDCT Method - (reply: 1)
85. qPCR data analysis from raw data - (reply: 1)
86. cDNA degradation within a week at -20? - (reply: 2)
87. Primer calculation for qPCR - final reaction - (reply: 1)
88. Sec. structure in primers - (reply: 1)
89. Differences in shape of melt curve - (reply: 4)
90. Amount of RNA for cDNA - (reply: 4)