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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. Is this primer-dimer peak in my melting curve? - (reply: 6)
62. Taq / Phusion mix - (reply: 3)
63. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
64. Dilution series between HKG and GOI - (reply: 7)
65. Reference gene(s) selection and validaton for qPCR - (reply: 2)
66. Is that normal the negative control showing signals? - (reply: 13)
67. qPCR cannot detect reaction. - (reply: 1)
68. website for primer design - (reply: 1)
69. PCR Efficiency over 150%! - (reply: 1)
70. problem of amplification - (reply: 2)
71. Rxn not working - amplify/linearize plasmid - (reply: 3)
72. qRT-PCR works one day, and not the next - (reply: 1)
73. PCR not working - (reply: 11)
74. an urgent problem with replicates - (reply: 10)
75. Site-directed mutagenesis: maximum substitution - (reply: 1)
76. Inter run calibration qRT-PCR - (reply: 2)
77. Primer design and alternative transcripts - (reply: 2)
78. qPCR amplification - (reply: 4)
79. Extremely desperate noob question: How do these PCR work? - (reply: 6)
80. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
81. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
82. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
83. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
84. Genomic DNA Isolation from Soft Agar Colonies - (reply: 3)
85. help with total RNA isolation - (reply: 1)
86. Need help to identify bands in AGE result - (reply: 3)
87. COLD-PCR (Dissociation Curve) via qPCR - (reply: 4)
88. how to hasten real-time PCR amplifications - (reply: 2)
89. Please please help me with my Phusion PCR. - (reply: 5)
90. Nanodrop vs Picogreen to quantify post-PCR product - (reply: 3)