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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
62. High background fluorescence detected before starting PCR - (reply: 3)
63. Real-time pcr - (reply: 1)
64. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
65. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
66. Combining standard error of normalised fold change values - Qbase method - (reply: 2)
67. What cycle is best for analysing data? - (reply: 2)
68. Mineral oil quality in PCR tubes - (reply: 5)
69. Internal control for absolute quantification - (reply: 3)
70. Diagnostic PCR troubleshooting - (reply: 2)
71. QPCR replicates issue - (reply: 1)
72. RT-PCR Kit's Validation - (reply: 1)
73. Rt pcr - (reply: 4)
74. Viral Quantification - (reply: 1)
75. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
76. Housekeeping genes - Cq values - (reply: 4)
77. typical mRNA test with huge fold change? Any suggestions - (reply: 1)
78. PCR Amplification Issues and Primer dimer - (reply: 4)
79. Two consecutive ATG - (reply: 2)
80. How to make PCR analysis using the 22DDCT Method - (reply: 1)
81. qPCR data analysis from raw data - (reply: 1)
82. cDNA degradation within a week at -20? - (reply: 2)
83. Primer calculation for qPCR - final reaction - (reply: 1)
84. Sec. structure in primers - (reply: 1)
85. Differences in shape of melt curve - (reply: 4)
86. Amount of RNA for cDNA - (reply: 4)
87. Difference in melting temperature - (reply: 5)
88. Changing PCR recipe halfway - (reply: 1)
89. PCR master mix containing primers and Taq - (reply: 4)
90. Primer working with some cDNA samples and not other - (reply: 2)