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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. how to hasten real-time PCR amplifications - (reply: 2)
62. Please please help me with my Phusion PCR. - (reply: 5)
63. Nanodrop vs Picogreen to quantify post-PCR product - (reply: 3)
64. Need no RT control for all genes? - (reply: 1)
65. Different annealing temp between HKG and target gene - (reply: 2)
66. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
67. help in long pcr - (reply: 1)
68. Reselling a supermix/enzyme in a commercial diagnostic kit - (reply: 2)
69. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
70. PCR inhibitor in template DNA - (reply: 3)
71. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
72. Problem with Real-time PCR results analysis - (reply: 1)
73. Rotor-Gene 6000 data analysis - (reply: 1)
74. PCR from protozoa DNA - (reply: 3)
75. Ligation-mediated PCR: Vent Polymerase, Why? - (reply: 6)
76. tool for comparing many primers pairs - (reply: 4)
77. PCR that leads to protein synthesis - (reply: 18)
78. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
79. Use of DMSO in General PCR - (reply: 1)
80. PCR product size confusion - (reply: 3)
81. Concentration specification in PCR - (reply: 3)
82. help us understand the run - (reply: 1)
83. Different MOI in comparison experiment - (reply: 3)
84. How to determine the size of gene to amplify? - (reply: 1)
85. Guanidine isothiocyanate in PCR - (reply: 1)
86. Untreated samples negative, how to analyze fold change? - (reply: 1)
87. Primers have worked well but now getting primer dimers? - (reply: 2)
88. I cannot design primers on exon-exon junction - (reply: 2)
89. DNA Quantification of PCR Products - (reply: 2)
90. In 2^ minus ddCt method, why is the "minus" sign? - (reply: 1)