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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. PCR contamination - (reply: 6)
62. RNA agarose gel blurry fuzzy. RNase contaminaton? - (reply: 2)
63. cDNA serial dilution trouble in qPCR: help needed - (reply: 2)
64. High background fluorescence detected before starting PCR - (reply: 3)
65. Real-time pcr - (reply: 1)
66. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
67. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
68. Combining standard error of normalised fold change values - Qbase method - (reply: 2)
69. What cycle is best for analysing data? - (reply: 2)
70. Mineral oil quality in PCR tubes - (reply: 5)
71. Internal control for absolute quantification - (reply: 3)
72. Diagnostic PCR troubleshooting - (reply: 2)
73. QPCR replicates issue - (reply: 1)
74. RT-PCR Kit's Validation - (reply: 1)
75. Rt pcr - (reply: 4)
76. Viral Quantification - (reply: 1)
77. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
78. Housekeeping genes - Cq values - (reply: 4)
79. typical mRNA test with huge fold change? Any suggestions - (reply: 1)
80. PCR Amplification Issues and Primer dimer - (reply: 4)
81. Two consecutive ATG - (reply: 2)
82. How to make PCR analysis using the 22DDCT Method - (reply: 1)
83. qPCR data analysis from raw data - (reply: 1)
84. cDNA degradation within a week at -20? - (reply: 2)
85. Primer calculation for qPCR - final reaction - (reply: 1)
86. Sec. structure in primers - (reply: 1)
87. Differences in shape of melt curve - (reply: 4)
88. Amount of RNA for cDNA - (reply: 4)
89. Difference in melting temperature - (reply: 5)
90. Changing PCR recipe halfway - (reply: 1)