Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
61. Taqman probe in a LightCycler 480? - (reply: 1)
62. Real time PCR for degraded RNA - (reply: 1)
63. Ct value of housekeeping gene - (reply: 4)
64. Efficiency correct fold change -- use all or none? - (reply: 1)
65. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
66. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
67. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
68. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
69. Is this primer-dimer peak in my melting curve? - (reply: 6)
70. Taq / Phusion mix - (reply: 3)
71. I'm working on an automated DD2CT value generator for Life tech qPCR machine - (reply: 2)
72. Dilution series between HKG and GOI - (reply: 7)
73. Reference gene(s) selection and validaton for qPCR - (reply: 2)
74. Is that normal the negative control showing signals? - (reply: 13)
75. qPCR cannot detect reaction. - (reply: 1)
76. website for primer design - (reply: 1)
77. PCR Efficiency over 150%! - (reply: 1)
78. problem of amplification - (reply: 2)
79. Rxn not working - amplify/linearize plasmid - (reply: 3)
80. qRT-PCR works one day, and not the next - (reply: 1)
81. PCR not working - (reply: 11)
82. an urgent problem with replicates - (reply: 10)
83. Site-directed mutagenesis: maximum substitution - (reply: 1)
84. Inter run calibration qRT-PCR - (reply: 2)
85. Primer design and alternative transcripts - (reply: 2)
86. qPCR amplification - (reply: 4)
87. Extremely desperate noob question: How do these PCR work? - (reply: 6)
88. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
89. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
90. RT-PCR - High Ct Values and Laser Capture - (reply: 1)