Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1381. How to know in which exon a primer match? - (reply: 1)
1382. dUTPs in qRTPCR rxn - (reply: 1)
1383. Amplification of human genomic DNA - (reply: 2)
1384. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1385. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1386. make your own gotaq - (reply: 2)
1387. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1388. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1389. primer RE over-hang nucleotides - common sequences? (reply: 5)
1390. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1391. Does RT step smplify RNA - (reply: 2)
1392. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1393. ROX utilization - (who uses ROX?) (reply: 1)
1394. qPCR analysis - (reply: 6)
1395. good housekeeping for ovary - (reply: 2)
1396. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
1397. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
1398. primer design problem - (reply: 1)
1399. The perfect RT??? which control? - (reply: 4)
1400. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
1401. qPCR - qPCR problems.. (reply: 5)
1402. is it necessary to do DNases treatment? - (reply: 2)
1403. problem with site directed mutagenesis - (reply: 4)
1404. allele specific pcr - determining genotypes (reply: 2)
1405. Low 260/230 ratio in my RNA - (reply: 3)
1406. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
1407. Problem with primer efficiency analysis - (reply: 4)
1408. calibrator - what is this in qPCR (reply: 1)
1409. slan real time PCR system for validation of microarray results? - (reply: 1)
1410. PCR unusualband at ~230bps - PCR (reply: 1)