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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1381. Dumb Reverse Transcription PCR Question - (reply: 2)
1382. qPCR absolute/relative quantification method question - (reply: 4)
1383. PCR-amplicon too short - (reply: 1)
1384. Reverse transcription PCR - (reply: 1)
1385. PCR/RT-PCR beads - (reply: 2)
1386. High Amplification Efficiency in Std. Curve - (reply: 5)
1387. Run PCR amplifications in agarose gels - (reply: 3)
1388. Problems with validating primers and low expression genes - (reply: 3)
1389. Compare different qPCR runs - really need help (reply: 8)
1390. General real time questions. - (reply: 1)
1391. Analysis HELP!!!! - (reply: 1)
1392. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
1393. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
1394. Mutagenesis woes - (reply: 3)
1395. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1396. intron/exon spanning primers - (reply: 1)
1397. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
1398. PCR product for sequencing - sample storage?? (reply: 2)
1399. Absolute quantification using Real Time PCR - (reply: 2)
1400. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
1401. High Ct value - what to do next? - (reply: 4)
1402. Biorad Mini Opticon - can use any 0.2ml tubes or Biorad only? (reply: 1)
1403. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
1404. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
1405. Designing Primers for multiple Isoforms - (reply: 1)
1406. GAPDH CT lower than target gene CT - (reply: 2)
1407. standards for absolute quantification qPCR - (reply: 1)
1408. RT-PCR standard curve dilutions - (reply: 2)
1409. more than one band - (reply: 1)
1410. Relative gene expression - Newbie at RT-qPCR! (reply: 3)