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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1381. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
1382. DATA ANALYSIS for qPCR - Calculating 2^(-Del Del Ct). (reply: 2)
1383. 18s control in qPCR - (reply: 2)
1384. weird PCR ask for help - (reply: 12)
1385. Absolute quantification and normalization - Are reference genes necessary? (reply: 1)
1386. What's the best electronic pipette (Precision) - (reply: 5)
1387. No PCR product at all - (reply: 8)
1388. PCR troubleshoooooting - primer dimer (reply: 1)
1389. Primer efficiency test - (reply: 1)
1390. primer software - (reply: 2)
1391. Question on wired PCR - (reply: 1)
1392. Validation of housekeeping gene expression - (reply: 3)
1393. restriction digestion of PCR product - (reply: 7)
1394. reuse pipette tips - (reply: 2)
1395. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
1396. How to know in which exon a primer match? - (reply: 1)
1397. dUTPs in qRTPCR rxn - (reply: 1)
1398. Amplification of human genomic DNA - (reply: 2)
1399. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1400. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1401. make your own gotaq - (reply: 2)
1402. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1403. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1404. primer RE over-hang nucleotides - common sequences? (reply: 5)
1405. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1406. Does RT step smplify RNA - (reply: 2)
1407. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1408. ROX utilization - (who uses ROX?) (reply: 1)
1409. qPCR analysis - (reply: 6)
1410. good housekeeping for ovary - (reply: 2)