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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1381. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
1382. intron/exon spanning primers - (reply: 1)
1383. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
1384. PCR product for sequencing - sample storage?? (reply: 2)
1385. Absolute quantification using Real Time PCR - (reply: 2)
1386. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
1387. High Ct value - what to do next? - (reply: 4)
1388. Biorad Mini Opticon - can use any 0.2ml tubes or Biorad only? (reply: 1)
1389. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
1390. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
1391. Designing Primers for multiple Isoforms - (reply: 1)
1392. GAPDH CT lower than target gene CT - (reply: 2)
1393. standards for absolute quantification qPCR - (reply: 1)
1394. RT-PCR standard curve dilutions - (reply: 2)
1395. more than one band - (reply: 1)
1396. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
1397. qPCR, RT- Control - (reply: 2)
1398. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
1399. real time pcr melt curve and primer efficiency problem - (reply: 3)
1400. real time PCR - base line not zero anymore! (reply: 5)
1401. Pre-amplification of template for qPCR - (reply: 1)
1402. Efficiency estimation using a window of linearity - (reply: 3)
1403. Software for analysis of raw real-time flouresence data - Analysis of tab delimited text flouresence v cycle data (reply: 7)
1404. Real time PCR - Standard curve construction (reply: 2)
1405. issue with taqman MGB probes - (reply: 2)
1406. PCR product dimer issue - (reply: 24)
1407. PCR additives Formamide - Formamide which one? (reply: 4)
1408. RNA from Zebrafish - (reply: 3)
1409. Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)
1410. conventional v real-time PCR applications - (reply: 1)