Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1381. PCR band too thick - PCR troubleshooting question (reply: 5)
1382. PCR-PIRA - (reply: 2)
1383. question qPCR data presentation? - (reply: 1)
1384. RT-PCR problem - (reply: 1)
1385. increasing qPCR reaction volume - Quick and simple question of qPCR reaction volume (reply: 2)
1386. Addition of a long sequence using PCR - HELP , PLEASE (reply: 1)
1387. Measuring gene expression across cell lines - (reply: 2)
1388. How to get enough product from limited samples without generating mutations? - (reply: 8)
1389. long range PCR - (reply: 2)
1390. ITS1/ITS4 and ITS1/ITS2 - (reply: 2)
1391. PCR off plasmid for screening - (reply: 2)
1392. BSA as qPCR enhancer - NEB or Sigma? (reply: 3)
1393. Primer clean-up - ExoSAP-It isn't working (reply: 6)
1394. Data acquisition for qPCR - Endpoint of annealing or endpoint of amplification? (reply: 3)
1395. Can't re-PCR the PCR product - (reply: 5)
1396. Contamination in Multilplex? - (reply: 4)
1397. some questions about the component tab of ABI prism 7000 software and the dissoc - (reply: 2)
1398. Optimal annealing temperature - (reply: 2)
1399. Little to no expression in one group, how to analyze this? - (reply: 1)
1400. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
1401. Taqman real-time PCR - Taqman and contamination (reply: 4)
1402. silly control question - No RT controls (reply: 2)
1403. Long Primers for PCR - (reply: 2)
1404. Data Analysis for Real-time PCR - (reply: 2)
1405. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
1406. argh more pcr headaches! - (reply: 6)
1407. Annealing temperature - (reply: 3)
1408. Replicate inconsistencies in qPCR - (reply: 6)
1409. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
1410. Chlamydia PCR cycling conditions - (reply: 1)