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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
871. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
872. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
873. Gene on negative strand - two eductional questions. - (reply: 3)
874. Newbie- choose DNA template - (reply: 1)
875. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
876. Total RNA estimation - (reply: 2)
877. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
878. pcr contamination - (reply: 4)
879. Large Non-specfic bands in PCR - (reply: 3)
880. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
881. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
882. Primer dilution --> problem.. - (reply: 16)
883. no bands after pcr - even after we have change most of the reagents (reply: 2)
884. High resolution melting to gene scanning - (reply: 1)
885. Help! Smear in Real-Time PCR Product - (reply: 1)
886. Is one reference gene ever appropriate? - (reply: 3)
887. Sybr green RT-qPCR primer - (reply: 1)
888. Sybr green RT-qPCR primer - (reply: 1)
889. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
890. Oligo Question - (reply: 1)
891. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
892. real time rt PCR - bloodyurine - inhibition - (reply: 1)
893. No Amplification in PCR - (reply: 4)
894. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
895. Real time PCR and percentage loss - (reply: 1)
896. Problem with smear in PCR with certain templates - (reply: 4)
897. running multiple qPCR with common housekeeping control - (reply: 3)
898. Problems with PCR in general - (reply: 1)
899. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
900. Control inconsistence in Q-PCR - (reply: 1)