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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
871. Really weird amplification curves - (reply: 1)
872. Primers for qPCR - (reply: 3)
873. copy number calculation from cDNA - (reply: 1)
874. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
875. RT-PCR primer - (reply: 3)
876. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
877. PCR problem - basic PCR for plasmid amplification (reply: 2)
878. can RNA amplify in two-step qPCR? - (reply: 2)
879. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
880. Nested LA PCR - Any success? (reply: 3)
881. Unpredictablity of PCR product - (reply: 5)
882. total cDNA amplification by PCR - (reply: 2)
883. Detection limit of a conventional PCR - (reply: 2)
884. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
885. RNAi showing upregulation via QPCR - Help needed (reply: 3)
886. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
887. cDNA synthesis - (reply: 3)
888. 2 rounds PCR got problem - (reply: 2)
889. PCR help minus strand - Primer design (reply: 1)
890. cDNA contamination, pls help! - (reply: 1)
891. Smear in long distance PCR - (reply: 39)
892. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
893. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
894. can contaminating gDNA explain this? - (reply: 1)
895. TOUCH DOWN PCR - (reply: 2)
896. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
897. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
898. PCR stopped working - After changing buffer (reply: 5)
899. how to determine the amount of cDNA for qPCR - (reply: 5)
900. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)