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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
871. Primer concentration - (reply: 4)
872. nested PCR with low target DNA? - (reply: 2)
873. RT-PCR calibrator - (reply: 2)
874. problem in pcr - (reply: 2)
875. Can someone recommend me a virtual PCR software? - (reply: 6)
876. negative values after GOI - reference gene ?? - (reply: 4)
877. RNA degradation during electrophoresis?? - (reply: 2)
878. Primer 3' mismatch - Strange experience (reply: 9)
879. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
880. Adding loading/tracking dye to PCR mix? - (reply: 10)
881. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
882. internal control in RT-PCR - inquiry (reply: 2)
883. Melting Temp of Standards Changed? - (reply: 3)
884. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
885. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
886. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
887. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
888. Gene on negative strand - two eductional questions. - (reply: 3)
889. Newbie- choose DNA template - (reply: 1)
890. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
891. Total RNA estimation - (reply: 2)
892. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
893. pcr contamination - (reply: 4)
894. Large Non-specfic bands in PCR - (reply: 3)
895. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
896. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
897. Primer dilution --> problem.. - (reply: 16)
898. no bands after pcr - even after we have change most of the reagents (reply: 2)
899. High resolution melting to gene scanning - (reply: 1)
900. Help! Smear in Real-Time PCR Product - (reply: 1)