Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
871. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
872. Gene on negative strand - two eductional questions. - (reply: 3)
873. Newbie- choose DNA template - (reply: 1)
874. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
875. Total RNA estimation - (reply: 2)
876. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
877. pcr contamination - (reply: 4)
878. Large Non-specfic bands in PCR - (reply: 3)
879. Pfaffl calculation for qpcr: what to enter for E value? - (reply: 2)
880. Distinguishing homozygous from hemizygous transformed plants - (reply: 13)
881. Primer dilution --> problem.. - (reply: 16)
882. no bands after pcr - even after we have change most of the reagents (reply: 2)
883. High resolution melting to gene scanning - (reply: 1)
884. Help! Smear in Real-Time PCR Product - (reply: 1)
885. Is one reference gene ever appropriate? - (reply: 3)
886. Sybr green RT-qPCR primer - (reply: 1)
887. Sybr green RT-qPCR primer - (reply: 1)
888. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
889. Oligo Question - (reply: 1)
890. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
891. real time rt PCR - bloodyurine - inhibition - (reply: 1)
892. No Amplification in PCR - (reply: 4)
893. DNase treatment after RNA extraction using RNeasy kit of qiagen - DNaes treatment after RNA extraction (reply: 2)
894. Real time PCR and percentage loss - (reply: 1)
895. Problem with smear in PCR with certain templates - (reply: 4)
896. running multiple qPCR with common housekeeping control - (reply: 3)
897. Problems with PCR in general - (reply: 1)
898. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
899. Control inconsistence in Q-PCR - (reply: 1)
900. how to overcome primer contamination - need protocol (reply: 11)