Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
871. Primers... - (reply: 1)
872. 18s as endogenous control for Oligo dT RT - Can we use 18s ? (reply: 2)
873. excel and propagation of error, qpcr - (reply: 1)
874. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
875. primer with higher melting temperature - (reply: 2)
876. Omitting Points in Standard Curve - (reply: 2)
877. Triage of miRNA targets by qPCR - (reply: 1)
878. Smallest reaction volume with Roche Lightcycler 480 96-well plate format - (reply: 2)
879. qPCR primer design - possible off-target (reply: 1)
880. Primer dimer formation and real-time PCR - (reply: 7)
881. 5' race - (reply: 2)
882. Identify PCR products - (reply: 3)
883. Maximum size of overhangs in PCR - (reply: 6)
884. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
885. a second small peak to the right to my pricipal peak!!! - (reply: 1)
886. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
887. Colony PCR doesn't work anymore - (reply: 5)
888. Question about TM and Annealing temperatures - (reply: 4)
889. RT-PCR t-test, and ANOVA - (reply: 1)
890. PCR basics - I'm new to all things lab and need some help! (reply: 3)
891. the red dye in trizol? - (reply: 2)
892. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
893. What are exogeneous and endogeneous controls? - (reply: 4)
894. quantitate wild type and mutant mRNA - (reply: 1)
895. Can very low RNA concentration cause Ct bias..? - (reply: 3)
896. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
897. iScript BioRad cDNA synthesis yield - (reply: 1)
898. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
899. intron spanning primers for non model organisms - (reply: 2)
900. Using DNA as a reference in qRT-PCR - (reply: 1)