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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
871. Nuclear or Cytoplasmic RNA Purification - (reply: 1)
872. >100% qPCR efficiency - (reply: 2)
873. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
874. PCR Primers - (reply: 2)
875. Cleaning up RT-PCR product before sequencing - (reply: 1)
876. reference gene - (reply: 2)
877. Improving qRT-PCR detection limits - (reply: 2)
878. What to use for Standard Curve - (reply: 2)
879. gPCR - (reply: 1)
880. RT-PCR cDNA synthesis - (reply: 6)
881. problems amplifying from cDNA - (reply: 4)
882. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
883. Primers... - (reply: 1)
884. 18s as endogenous control for Oligo dT RT - Can we use 18s ? (reply: 2)
885. excel and propagation of error, qpcr - (reply: 1)
886. Reverse transcription (cDNA synthesis) curiosity and confusion - Does the amount of RNA have to be doubled if you double everything els (reply: 4)
887. primer with higher melting temperature - (reply: 2)
888. Omitting Points in Standard Curve - (reply: 2)
889. Triage of miRNA targets by qPCR - (reply: 1)
890. Smallest reaction volume with Roche Lightcycler 480 96-well plate format - (reply: 2)
891. qPCR primer design - possible off-target (reply: 1)
892. Primer dimer formation and real-time PCR - (reply: 7)
893. 5' race - (reply: 2)
894. Identify PCR products - (reply: 3)
895. Maximum size of overhangs in PCR - (reply: 6)
896. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
897. a second small peak to the right to my pricipal peak!!! - (reply: 1)
898. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
899. Colony PCR doesn't work anymore - (reply: 5)
900. Question about TM and Annealing temperatures - (reply: 4)