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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
871. What can I do to increase the banding signal? - (reply: 6)
872. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
873. Tomato Actin PCR+Images included - (reply: 2)
874. single primer PCR - (reply: 5)
875. Weird qPCR curves - (reply: 4)
876. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
877. LacZ PCR Problems - (reply: 1)
878. Primer concentration - (reply: 4)
879. nested PCR with low target DNA? - (reply: 2)
880. RT-PCR calibrator - (reply: 2)
881. problem in pcr - (reply: 2)
882. Can someone recommend me a virtual PCR software? - (reply: 6)
883. negative values after GOI - reference gene ?? - (reply: 4)
884. RNA degradation during electrophoresis?? - (reply: 2)
885. Primer 3' mismatch - Strange experience (reply: 9)
886. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
887. Adding loading/tracking dye to PCR mix? - (reply: 10)
888. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
889. internal control in RT-PCR - inquiry (reply: 2)
890. Melting Temp of Standards Changed? - (reply: 3)
891. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
892. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
893. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
894. genes having different annealing temperatures in the same qPCR run - housekeeping gene and target gene have different annealing temperature (reply: 1)
895. Gene on negative strand - two eductional questions. - (reply: 3)
896. Newbie- choose DNA template - (reply: 1)
897. Help -Water Blank keeps coming up with mutiple bands - (reply: 8)
898. Total RNA estimation - (reply: 2)
899. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
900. pcr contamination - (reply: 4)