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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
871. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
872. qPCR volume - Problem with volume difference and results (reply: 1)
873. RT-PCR - (reply: 9)
874. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
875. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
876. cDNA synthesis - (reply: 6)
877. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
878. Primer seq - (reply: 2)
879. Selection of housekeeping gene in Mycobacterium - (reply: 1)
880. Do I need to pool the base line samples together - (reply: 2)
881. PCR product size - (reply: 6)
882. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
883. Few basic questions about RNA - (reply: 3)
884. oligo dt and random primer - (reply: 3)
885. Ct's of goi higher and lower than ct's hkg - (reply: 1)
886. Effect of collagenase on qpcr results - (reply: 1)
887. RNA Isolation - (reply: 3)
888. mRNA Search for RT-PCR (U to T) - (reply: 2)
889. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
890. PCR triplicates versus one reaction - differences? (reply: 17)
891. cloning of race products - (reply: 1)
892. Single or double-stranded cDNA? - (reply: 2)
893. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
894. qPCR inhibition - (reply: 1)
895. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
896. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
897. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
898. Primers and self and hetero dimers - (reply: 1)
899. Really weird amplification curves - (reply: 1)
900. Primers for qPCR - (reply: 3)