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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. minimum replicate for qpcr? - (reply: 15)
962. Primer design - (reply: 2)
963. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
964. qPCR amplification interference fixed with freezing - (reply: 6)
965. Suggest me a mastermix for conventinal PCR... - (reply: 11)
966. reaction efficiency - (reply: 4)
967. software for quantifing PCR product band - (reply: 1)
968. Thermal cycler - (reply: 2)
969. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
970. qPCR volume - Problem with volume difference and results (reply: 1)
971. RT-PCR - (reply: 9)
972. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
973. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
974. cDNA synthesis - (reply: 6)
975. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
976. Primer seq - (reply: 2)
977. Selection of housekeeping gene in Mycobacterium - (reply: 1)
978. Do I need to pool the base line samples together - (reply: 2)
979. PCR product size - (reply: 6)
980. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
981. Few basic questions about RNA - (reply: 3)
982. oligo dt and random primer - (reply: 3)
983. Ct's of goi higher and lower than ct's hkg - (reply: 1)
984. Effect of collagenase on qpcr results - (reply: 1)
985. RNA Isolation - (reply: 3)
986. mRNA Search for RT-PCR (U to T) - (reply: 2)
987. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
988. PCR triplicates versus one reaction - differences? (reply: 17)
989. cloning of race products - (reply: 1)
990. Single or double-stranded cDNA? - (reply: 2)