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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. Primer dimer formation and real-time PCR - (reply: 7)
962. 5' race - (reply: 2)
963. Identify PCR products - (reply: 3)
964. Maximum size of overhangs in PCR - (reply: 6)
965. water-droplets in my cycler !!! - experienced such thing ??? (reply: 9)
966. a second small peak to the right to my pricipal peak!!! - (reply: 1)
967. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
968. Colony PCR doesn't work anymore - (reply: 5)
969. Question about TM and Annealing temperatures - (reply: 4)
970. RT-PCR t-test, and ANOVA - (reply: 1)
971. PCR basics - I'm new to all things lab and need some help! (reply: 3)
972. the red dye in trizol? - (reply: 2)
973. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
974. What are exogeneous and endogeneous controls? - (reply: 4)
975. quantitate wild type and mutant mRNA - (reply: 1)
976. Can very low RNA concentration cause Ct bias..? - (reply: 3)
977. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
978. iScript BioRad cDNA synthesis yield - (reply: 1)
979. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
980. intron spanning primers for non model organisms - (reply: 2)
981. Using DNA as a reference in qRT-PCR - (reply: 1)
982. minimum replicate for qpcr? - (reply: 15)
983. Primer design - (reply: 2)
984. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
985. qPCR amplification interference fixed with freezing - (reply: 6)
986. Suggest me a mastermix for conventinal PCR... - (reply: 11)
987. reaction efficiency - (reply: 4)
988. software for quantifing PCR product band - (reply: 1)
989. Thermal cycler - (reply: 2)
990. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)