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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
962. Standardization of HRM for methylation analysis - (reply: 4)
963. PCR protocol questions! - (reply: 2)
964. standard curve for qPCR - (reply: 3)
965. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
966. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
967. DNA amount calculation for PCR - (reply: 14)
968. ChIP primer design - (reply: 1)
969. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
970. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
971. weird QPCR curves - (reply: 2)
972. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
973. Questions regarding RT-PCR optimization - (reply: 2)
974. Problems with semi-quatitative PCR - (reply: 1)
975. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
976. Long-amplicon QPCR of mtDNA - (reply: 5)
977. PCR reaction calculation - (reply: 2)
978. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
979. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
980. no increase in fluorescence in my real time PCR - (reply: 3)
981. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
982. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
983. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
984. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
985. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
986. PCR, using gel extracted band as a template - (reply: 4)
987. Question of nucleic acid extraction - (reply: 1)
988. Multiplex problems - (reply: 6)
989. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
990. Q-PCR and RT-PCR - (reply: 3)