Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. TOUCH DOWN PCR - (reply: 2)
962. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
963. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
964. PCR stopped working - After changing buffer (reply: 5)
965. how to determine the amount of cDNA for qPCR - (reply: 5)
966. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
967. Standardization of HRM for methylation analysis - (reply: 4)
968. PCR protocol questions! - (reply: 2)
969. standard curve for qPCR - (reply: 3)
970. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
971. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
972. DNA amount calculation for PCR - (reply: 14)
973. ChIP primer design - (reply: 1)
974. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
975. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
976. weird QPCR curves - (reply: 2)
977. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
978. Questions regarding RT-PCR optimization - (reply: 2)
979. Problems with semi-quatitative PCR - (reply: 1)
980. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
981. Long-amplicon QPCR of mtDNA - (reply: 5)
982. PCR reaction calculation - (reply: 2)
983. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
984. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
985. no increase in fluorescence in my real time PCR - (reply: 3)
986. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
987. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
988. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
989. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
990. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)