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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. oligo dt and random primer - (reply: 3)
962. Ct's of goi higher and lower than ct's hkg - (reply: 1)
963. Effect of collagenase on qpcr results - (reply: 1)
964. RNA Isolation - (reply: 3)
965. mRNA Search for RT-PCR (U to T) - (reply: 2)
966. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
967. PCR triplicates versus one reaction - differences? (reply: 17)
968. cloning of race products - (reply: 1)
969. Single or double-stranded cDNA? - (reply: 2)
970. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
971. qPCR inhibition - (reply: 1)
972. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
973. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
974. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
975. Primers and self and hetero dimers - (reply: 1)
976. Really weird amplification curves - (reply: 1)
977. Primers for qPCR - (reply: 3)
978. copy number calculation from cDNA - (reply: 1)
979. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
980. RT-PCR primer - (reply: 3)
981. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
982. PCR problem - basic PCR for plasmid amplification (reply: 2)
983. can RNA amplify in two-step qPCR? - (reply: 2)
984. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
985. Nested LA PCR - Any success? (reply: 3)
986. Unpredictablity of PCR product - (reply: 5)
987. total cDNA amplification by PCR - (reply: 2)
988. Detection limit of a conventional PCR - (reply: 2)
989. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
990. RNAi showing upregulation via QPCR - Help needed (reply: 3)