Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. Detection limit of a conventional PCR - (reply: 2)
962. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
963. RNAi showing upregulation via QPCR - Help needed (reply: 3)
964. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
965. cDNA synthesis - (reply: 3)
966. 2 rounds PCR got problem - (reply: 2)
967. PCR help minus strand - Primer design (reply: 1)
968. cDNA contamination, pls help! - (reply: 1)
969. Smear in long distance PCR - (reply: 39)
970. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
971. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
972. can contaminating gDNA explain this? - (reply: 1)
973. TOUCH DOWN PCR - (reply: 2)
974. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
975. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
976. PCR stopped working - After changing buffer (reply: 5)
977. how to determine the amount of cDNA for qPCR - (reply: 5)
978. Problems with SYBR Green assay - bad melting curves and bad amp efficiency (reply: 12)
979. Standardization of HRM for methylation analysis - (reply: 4)
980. PCR protocol questions! - (reply: 2)
981. standard curve for qPCR - (reply: 3)
982. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
983. About serial dilution of cDNA were amplified by real-time PCR - How to do serial dilution of cDNA (reply: 1)
984. DNA amount calculation for PCR - (reply: 14)
985. ChIP primer design - (reply: 1)
986. QPCR melting curves peaks - Standard curve has one peak, samples have another (reply: 5)
987. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
988. weird QPCR curves - (reply: 2)
989. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
990. Questions regarding RT-PCR optimization - (reply: 2)