Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
962. delta-delta-Ct method - what to do, when no expression in healthy control? (reply: 1)
963. Trouble with annealing temperature - Trouble with annealing temperature (reply: 3)
964. PCR Master mix - (reply: 2)
965. Real Time PCR Primers and Probes - (reply: 2)
966. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
967. Dead genotyping PCR - I can't see s*** (reply: 5)
968. Any one has light cycler 480 system operator's mannual? - Urgent!!!!!!!!!!!! (reply: 3)
969. PCR product wrong size! Need help! - (reply: 9)
970. pooling different pcr cycles? - (reply: 1)
971. Amplification of concatenated linear ligated fragments - (reply: 4)
972. Clinical Real-time PCR assay - (reply: 1)
973. Alternative software to read .sds files? - (reply: 2)
974. primer and promoter sequences - (reply: 1)
975. Standard curve for real-time PCR - (reply: 2)
976. delta Ct values - (reply: 1)
977. Primer Tm differences/ PCR - (reply: 1)
978. Interpretation of Ct values - (reply: 2)
979. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
980. Question about RT... - (reply: 1)
981. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
982. PCR Primer Dilution from F+R - (reply: 1)
983. Plasmid standard problem - (reply: 2)
984. primer reconstitution - primer reconstitution (reply: 4)
985. Not-Quite-Nested PCR - (reply: 2)
986. Primer design for insert amplification - Is this correct? (reply: 2)
987. got smear on the PCR gel !!! - (reply: 4)
988. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
989. Troubleshooting reverse transcriptase-PCR - (reply: 3)
990. "Unzipped" PCR band - (reply: 3)