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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. PCR, using gel extracted band as a template - (reply: 4)
962. Question of nucleic acid extraction - (reply: 1)
963. Multiplex problems - (reply: 6)
964. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
965. Q-PCR and RT-PCR - (reply: 3)
966. inter-run calibration - (reply: 4)
967. Delta Delta Ct method for multiple reference genes? - (reply: 1)
968. FAM and VIC labelling - (reply: 2)
969. Lost cause?? - I made a silly (reply: 4)
970. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
971. confusing PCR - (reply: 1)
972. difference between PCR primers and sequencing primers - (reply: 1)
973. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
974. making gel electrophoresis - (reply: 1)
975. housekeeping genes in PCR - (reply: 1)
976. Question about RealTime PCR Primer Design - (reply: 1)
977. Primers stopped working!? - PCR Primers (reply: 6)
978. NFK beta primers - (reply: 4)
979. final PCR product - need clarifications (reply: 1)
980. Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su - Hi, I'm trying to sequence a gene from the cDNA of an insect (reply: 2)
981. Can I use Hot Start Taq polimerase for any target? - (reply: 6)
982. Ct value and interpretation - (reply: 3)
983. 16S rDNA - Bacteria identification (reply: 1)
984. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
985. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
986. gene expressional analysis - (reply: 1)
987. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
988. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
989. why genomic can't be use for pcr? - (reply: 2)
990. Re-use unamplified PCR product - (reply: 2)