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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. PCR basics - I'm new to all things lab and need some help! (reply: 3)
962. the red dye in trizol? - (reply: 2)
963. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
964. What are exogeneous and endogeneous controls? - (reply: 4)
965. quantitate wild type and mutant mRNA - (reply: 1)
966. Can very low RNA concentration cause Ct bias..? - (reply: 3)
967. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
968. iScript BioRad cDNA synthesis yield - (reply: 1)
969. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
970. intron spanning primers for non model organisms - (reply: 2)
971. Using DNA as a reference in qRT-PCR - (reply: 1)
972. minimum replicate for qpcr? - (reply: 15)
973. Primer design - (reply: 2)
974. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
975. qPCR amplification interference fixed with freezing - (reply: 6)
976. Suggest me a mastermix for conventinal PCR... - (reply: 11)
977. reaction efficiency - (reply: 4)
978. software for quantifing PCR product band - (reply: 1)
979. Thermal cycler - (reply: 2)
980. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
981. qPCR volume - Problem with volume difference and results (reply: 1)
982. RT-PCR - (reply: 9)
983. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
984. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
985. cDNA synthesis - (reply: 6)
986. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
987. Primer seq - (reply: 2)
988. Selection of housekeeping gene in Mycobacterium - (reply: 1)
989. Do I need to pool the base line samples together - (reply: 2)
990. PCR product size - (reply: 6)