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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
962. weird QPCR curves - (reply: 2)
963. how many minimum and maximum number of bands in SSCP marker? - (reply: 2)
964. Questions regarding RT-PCR optimization - (reply: 2)
965. Problems with semi-quatitative PCR - (reply: 1)
966. conversion of total RNA to cDNA - problems in total RNA to cDNA (reply: 7)
967. Long-amplicon QPCR of mtDNA - (reply: 5)
968. PCR reaction calculation - (reply: 2)
969. Qiagen rotorgene syber green kit reaction volume - (reply: 1)
970. missing nt at the 5' end - site mutagensis using NEB kit (reply: 1)
971. no increase in fluorescence in my real time PCR - (reply: 3)
972. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
973. Roche second derivative maximum - can be treated according to Pfaffl? (reply: 1)
974. Attenuation of qPCR efficiency? - Established method for reducing efficiency of qPCR reaction? (reply: 3)
975. Pooled RNA from healthy controls - delta-delta Ct method (reply: 1)
976. Probe Dilution Problem = 100uM to 5ng/ul? + Final Volume for FISH - Probe dilution and FISH - Advise on volume for FISH hibridization buff (reply: 2)
977. PCR, using gel extracted band as a template - (reply: 4)
978. Question of nucleic acid extraction - (reply: 1)
979. Multiplex problems - (reply: 6)
980. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
981. Q-PCR and RT-PCR - (reply: 3)
982. inter-run calibration - (reply: 4)
983. Delta Delta Ct method for multiple reference genes? - (reply: 1)
984. FAM and VIC labelling - (reply: 2)
985. Lost cause?? - I made a silly (reply: 4)
986. how to use delta-delta Ct method? - when no expression in healthy controls! (reply: 2)
987. confusing PCR - (reply: 1)
988. difference between PCR primers and sequencing primers - (reply: 1)
989. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
990. making gel electrophoresis - (reply: 1)