Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. Can very low RNA concentration cause Ct bias..? - (reply: 3)
962. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
963. iScript BioRad cDNA synthesis yield - (reply: 1)
964. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
965. intron spanning primers for non model organisms - (reply: 2)
966. Using DNA as a reference in qRT-PCR - (reply: 1)
967. minimum replicate for qpcr? - (reply: 15)
968. Primer design - (reply: 2)
969. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
970. qPCR amplification interference fixed with freezing - (reply: 6)
971. Suggest me a mastermix for conventinal PCR... - (reply: 11)
972. reaction efficiency - (reply: 4)
973. software for quantifing PCR product band - (reply: 1)
974. Thermal cycler - (reply: 2)
975. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
976. qPCR volume - Problem with volume difference and results (reply: 1)
977. RT-PCR - (reply: 9)
978. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
979. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
980. cDNA synthesis - (reply: 6)
981. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
982. Primer seq - (reply: 2)
983. Selection of housekeeping gene in Mycobacterium - (reply: 1)
984. Do I need to pool the base line samples together - (reply: 2)
985. PCR product size - (reply: 6)
986. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
987. Few basic questions about RNA - (reply: 3)
988. oligo dt and random primer - (reply: 3)
989. Ct's of goi higher and lower than ct's hkg - (reply: 1)
990. Effect of collagenase on qpcr results - (reply: 1)