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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
962. intron spanning primers for non model organisms - (reply: 2)
963. Using DNA as a reference in qRT-PCR - (reply: 1)
964. minimum replicate for qpcr? - (reply: 15)
965. Primer design - (reply: 2)
966. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
967. qPCR amplification interference fixed with freezing - (reply: 6)
968. Suggest me a mastermix for conventinal PCR... - (reply: 11)
969. reaction efficiency - (reply: 4)
970. software for quantifing PCR product band - (reply: 1)
971. Thermal cycler - (reply: 2)
972. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
973. qPCR volume - Problem with volume difference and results (reply: 1)
974. RT-PCR - (reply: 9)
975. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
976. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
977. cDNA synthesis - (reply: 6)
978. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
979. Primer seq - (reply: 2)
980. Selection of housekeeping gene in Mycobacterium - (reply: 1)
981. Do I need to pool the base line samples together - (reply: 2)
982. PCR product size - (reply: 6)
983. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
984. Few basic questions about RNA - (reply: 3)
985. oligo dt and random primer - (reply: 3)
986. Ct's of goi higher and lower than ct's hkg - (reply: 1)
987. Effect of collagenase on qpcr results - (reply: 1)
988. RNA Isolation - (reply: 3)
989. mRNA Search for RT-PCR (U to T) - (reply: 2)
990. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)