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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. RNA Isolation - (reply: 3)
962. mRNA Search for RT-PCR (U to T) - (reply: 2)
963. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
964. PCR triplicates versus one reaction - differences? (reply: 17)
965. cloning of race products - (reply: 1)
966. Single or double-stranded cDNA? - (reply: 2)
967. Comparing SYBR and probe-based Ct values - Is it valid? (reply: 2)
968. qPCR inhibition - (reply: 1)
969. what reference gene will you use to compare neuroblastoma, glioblastoma? - (reply: 1)
970. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
971. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
972. Primers and self and hetero dimers - (reply: 1)
973. Really weird amplification curves - (reply: 1)
974. Primers for qPCR - (reply: 3)
975. copy number calculation from cDNA - (reply: 1)
976. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
977. RT-PCR primer - (reply: 3)
978. CT value>25 with GAPDH in 50-fold dilution! - (reply: 3)
979. PCR problem - basic PCR for plasmid amplification (reply: 2)
980. can RNA amplify in two-step qPCR? - (reply: 2)
981. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
982. Nested LA PCR - Any success? (reply: 3)
983. Unpredictablity of PCR product - (reply: 5)
984. total cDNA amplification by PCR - (reply: 2)
985. Detection limit of a conventional PCR - (reply: 2)
986. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
987. RNAi showing upregulation via QPCR - Help needed (reply: 3)
988. DNA detection limit of the PCR - Calculating detection limits (reply: 4)
989. cDNA synthesis - (reply: 3)
990. 2 rounds PCR got problem - (reply: 2)