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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
961. qPCR virgin, What do I do first? - How to set up experiment (reply: 1)
962. Colony PCR doesn't work anymore - (reply: 5)
963. Question about TM and Annealing temperatures - (reply: 4)
964. RT-PCR t-test, and ANOVA - (reply: 1)
965. PCR basics - I'm new to all things lab and need some help! (reply: 3)
966. the red dye in trizol? - (reply: 2)
967. Is it acceptable that qpcr reaction doesn't reach plateau? - (reply: 3)
968. What are exogeneous and endogeneous controls? - (reply: 4)
969. quantitate wild type and mutant mRNA - (reply: 1)
970. Can very low RNA concentration cause Ct bias..? - (reply: 3)
971. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
972. iScript BioRad cDNA synthesis yield - (reply: 1)
973. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
974. intron spanning primers for non model organisms - (reply: 2)
975. Using DNA as a reference in qRT-PCR - (reply: 1)
976. minimum replicate for qpcr? - (reply: 15)
977. Primer design - (reply: 2)
978. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
979. qPCR amplification interference fixed with freezing - (reply: 6)
980. Suggest me a mastermix for conventinal PCR... - (reply: 11)
981. reaction efficiency - (reply: 4)
982. software for quantifing PCR product band - (reply: 1)
983. Thermal cycler - (reply: 2)
984. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
985. qPCR volume - Problem with volume difference and results (reply: 1)
986. RT-PCR - (reply: 9)
987. Best way to mix qPCR master mix and high ct deviation questions - (reply: 3)
988. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
989. cDNA synthesis - (reply: 6)
990. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)