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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1411. No PCR product at all - (reply: 8)
1412. PCR troubleshoooooting - primer dimer (reply: 1)
1413. Primer efficiency test - (reply: 1)
1414. primer software - (reply: 2)
1415. Question on wired PCR - (reply: 1)
1416. Validation of housekeeping gene expression - (reply: 3)
1417. restriction digestion of PCR product - (reply: 7)
1418. reuse pipette tips - (reply: 2)
1419. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
1420. How to know in which exon a primer match? - (reply: 1)
1421. dUTPs in qRTPCR rxn - (reply: 1)
1422. Amplification of human genomic DNA - (reply: 2)
1423. Relative qPCR dCt analysis - Different ways to analyze data? (reply: 1)
1424. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
1425. make your own gotaq - (reply: 2)
1426. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
1427. isolation of RNA from insect cells - to isolate RNA from insect larvae and synthesize cDNA (reply: 2)
1428. primer RE over-hang nucleotides - common sequences? (reply: 5)
1429. Forward and reverse primers got very different Tm - what to do? (reply: 11)
1430. Does RT step smplify RNA - (reply: 2)
1431. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
1432. ROX utilization - (who uses ROX?) (reply: 1)
1433. qPCR analysis - (reply: 6)
1434. good housekeeping for ovary - (reply: 2)
1435. Can I use genomic DNA to make a standard curve for qPCR - (reply: 5)
1436. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
1437. primer design problem - (reply: 1)
1438. The perfect RT??? which control? - (reply: 4)
1439. Rotor Gene 3000 and Rotor Gene Q - (reply: 1)
1440. qPCR - qPCR problems.. (reply: 5)