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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1411. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
1412. Chlamydia PCR cycling conditions - (reply: 1)
1413. On-unit screen or PC…which is better? - (reply: 12)
1414. Primers going "loopy" - (reply: 2)
1415. Biotinylated! - brief explanaion (reply: 2)
1416. what do i say?! - (reply: 2)
1417. Smearing on gel in PCR products - (reply: 5)
1418. 260/230 ratio: how low is good for running PCRs? - (reply: 2)
1419. introducing alanine mutations - (reply: 1)
1420. Hematin - calculation (reply: 1)
1421. How to generate sticky ends? - (reply: 3)
1422. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1423. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1424. Relative RT-PCR, multiplate and calibration - (reply: 4)
1425. Trying to measure small levels of mRNA - (reply: 3)
1426. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1427. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
1428. Relative RT-PCR - (reply: 2)
1429. Relative real time RT PCR - (reply: 2)
1430. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1431. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1432. IQ5 and sybr/FAM - (reply: 3)
1433. a simple primer problem - (reply: 5)
1434. Failure to introduce mutations using Overlap PCR - (reply: 3)
1435. no or poor amplification - (reply: 1)
1436. Primers from 3'UTR - (reply: 2)
1437. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
1438. Designing primers with ESTs - (reply: 2)
1439. Ct variation/Ct limit/r˛ values/efficiency errors - (reply: 2)
1440. Probe manufacturers, who makes best probes? - Where do you get your probes and are you happy with them? (reply: 3)