Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1411. Contamination in Multilplex? - (reply: 4)
1412. some questions about the component tab of ABI prism 7000 software and the dissoc - (reply: 2)
1413. Optimal annealing temperature - (reply: 2)
1414. Little to no expression in one group, how to analyze this? - (reply: 1)
1415. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
1416. Taqman real-time PCR - Taqman and contamination (reply: 4)
1417. silly control question - No RT controls (reply: 2)
1418. Long Primers for PCR - (reply: 2)
1419. Data Analysis for Real-time PCR - (reply: 2)
1420. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
1421. argh more pcr headaches! - (reply: 6)
1422. Annealing temperature - (reply: 3)
1423. Replicate inconsistencies in qPCR - (reply: 6)
1424. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
1425. Chlamydia PCR cycling conditions - (reply: 1)
1426. On-unit screen or PC…which is better? - (reply: 12)
1427. Primers going "loopy" - (reply: 2)
1428. Biotinylated! - brief explanaion (reply: 2)
1429. what do i say?! - (reply: 2)
1430. Smearing on gel in PCR products - (reply: 5)
1431. 260/230 ratio: how low is good for running PCRs? - (reply: 2)
1432. introducing alanine mutations - (reply: 1)
1433. Hematin - calculation (reply: 1)
1434. How to generate sticky ends? - (reply: 3)
1435. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1436. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1437. Relative RT-PCR, multiplate and calibration - (reply: 4)
1438. Trying to measure small levels of mRNA - (reply: 3)
1439. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1440. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)