|
1411. Re-PCR problem - (reply: 3)
1412. PCR and gel purification problem - did I screw this up (reply: 4)
1413. touchdown pcr - (reply: 6)
1414. non specific pcr products - (reply: 4)
1415. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
1416. template concentration qPCR - (reply: 1)
1417. Anyone with experiences in 2 step PCR - (reply: 1)
1418. qRT-PCR Problems - (reply: 1)
1419. PCR works on lab strain but not patient sample - HIV related (reply: 4)
1420. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
1421. Genomic DNA copy number qPCR - Help with control (reply: 8)
1422. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
1423. re-amplification of a PCR product - is it recommended? (reply: 7)
1424. PCR machine with accurate temperature control? - (reply: 1)
1425. qRT-PCR with DMSO - (reply: 2)
1426. Gene-specific RT-PCR - (reply: 2)
1427. Gain! - (reply: 4)
1428. selection of internal control gene - (reply: 2)
1429. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1430. primer design - primer design question (reply: 2)
1431. Protocol for 16S rDNA real time PCR - (reply: 2)
1432. qPCR - Standard curves (reply: 3)
1433. cDNA spectrophotometric quantification - (reply: 8)
1434. Standard curve standardization - variation between runs (reply: 4)
1435. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1436. real time PCR primer designing problems,again - is NCBI's primer blast believable enough？ (reply: 3)
1437. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1438. low gc primers - help with pcr using low gc primers (reply: 6)
1439. Exon-Exon Junction ? - (reply: 3)
1440. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
1412. PCR and gel purification problem - did I screw this up (reply: 4)
1413. touchdown pcr - (reply: 6)
1414. non specific pcr products - (reply: 4)
1415. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
1416. template concentration qPCR - (reply: 1)
1417. Anyone with experiences in 2 step PCR - (reply: 1)
1418. qRT-PCR Problems - (reply: 1)
1419. PCR works on lab strain but not patient sample - HIV related (reply: 4)
1420. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
1421. Genomic DNA copy number qPCR - Help with control (reply: 8)
1422. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
1423. re-amplification of a PCR product - is it recommended? (reply: 7)
1424. PCR machine with accurate temperature control? - (reply: 1)
1425. qRT-PCR with DMSO - (reply: 2)
1426. Gene-specific RT-PCR - (reply: 2)
1427. Gain! - (reply: 4)
1428. selection of internal control gene - (reply: 2)
1429. 2-ΔΔCT interpretation of gene copy number - What does a 2-ΔΔCT value of 1.84 or 0.7 mean? (reply: 3)
1430. primer design - primer design question (reply: 2)
1431. Protocol for 16S rDNA real time PCR - (reply: 2)
1432. qPCR - Standard curves (reply: 3)
1433. cDNA spectrophotometric quantification - (reply: 8)
1434. Standard curve standardization - variation between runs (reply: 4)
1435. How to recognize primer-dimers? - from a melting curve??... (reply: 4)
1436. real time PCR primer designing problems,again - is NCBI's primer blast believable enough？ (reply: 3)
1437. real time curve shifted by ~10 cycles - higher Cts than I used to (reply: 2)
1438. low gc primers - help with pcr using low gc primers (reply: 6)
1439. Exon-Exon Junction ? - (reply: 3)
1440. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)