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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1411. Data Analysis for Real-time PCR - (reply: 2)
1412. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
1413. argh more pcr headaches! - (reply: 6)
1414. Annealing temperature - (reply: 3)
1415. Replicate inconsistencies in qPCR - (reply: 6)
1416. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
1417. Chlamydia PCR cycling conditions - (reply: 1)
1418. On-unit screen or PC…which is better? - (reply: 12)
1419. Primers going "loopy" - (reply: 2)
1420. Biotinylated! - brief explanaion (reply: 2)
1421. what do i say?! - (reply: 2)
1422. Smearing on gel in PCR products - (reply: 5)
1423. 260/230 ratio: how low is good for running PCRs? - (reply: 2)
1424. introducing alanine mutations - (reply: 1)
1425. Hematin - calculation (reply: 1)
1426. How to generate sticky ends? - (reply: 3)
1427. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1428. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1429. Relative RT-PCR, multiplate and calibration - (reply: 4)
1430. Trying to measure small levels of mRNA - (reply: 3)
1431. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1432. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
1433. Relative RT-PCR - (reply: 2)
1434. Relative real time RT PCR - (reply: 2)
1435. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1436. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1437. IQ5 and sybr/FAM - (reply: 3)
1438. a simple primer problem - (reply: 5)
1439. Failure to introduce mutations using Overlap PCR - (reply: 3)
1440. no or poor amplification - (reply: 1)