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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1411. Addition of a long sequence using PCR - HELP , PLEASE (reply: 1)
1412. Measuring gene expression across cell lines - (reply: 2)
1413. How to get enough product from limited samples without generating mutations? - (reply: 8)
1414. long range PCR - (reply: 2)
1415. ITS1/ITS4 and ITS1/ITS2 - (reply: 2)
1416. PCR off plasmid for screening - (reply: 2)
1417. BSA as qPCR enhancer - NEB or Sigma? (reply: 3)
1418. Primer clean-up - ExoSAP-It isn't working (reply: 6)
1419. Data acquisition for qPCR - Endpoint of annealing or endpoint of amplification? (reply: 3)
1420. Can't re-PCR the PCR product - (reply: 5)
1421. Contamination in Multilplex? - (reply: 4)
1422. some questions about the component tab of ABI prism 7000 software and the dissoc - (reply: 2)
1423. Optimal annealing temperature - (reply: 2)
1424. Little to no expression in one group, how to analyze this? - (reply: 1)
1425. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
1426. Taqman real-time PCR - Taqman and contamination (reply: 4)
1427. silly control question - No RT controls (reply: 2)
1428. Long Primers for PCR - (reply: 2)
1429. Data Analysis for Real-time PCR - (reply: 2)
1430. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
1431. argh more pcr headaches! - (reply: 6)
1432. Annealing temperature - (reply: 3)
1433. Replicate inconsistencies in qPCR - (reply: 6)
1434. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
1435. Chlamydia PCR cycling conditions - (reply: 1)
1436. On-unit screen or PC…which is better? - (reply: 12)
1437. Primers going "loopy" - (reply: 2)
1438. Biotinylated! - brief explanaion (reply: 2)
1439. what do i say?! - (reply: 2)
1440. Smearing on gel in PCR products - (reply: 5)