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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1411. On-unit screen or PC…which is better? - (reply: 12)
1412. Primers going "loopy" - (reply: 2)
1413. Biotinylated! - brief explanaion (reply: 2)
1414. what do i say?! - (reply: 2)
1415. Smearing on gel in PCR products - (reply: 5)
1416. 260/230 ratio: how low is good for running PCRs? - (reply: 2)
1417. introducing alanine mutations - (reply: 1)
1418. Hematin - calculation (reply: 1)
1419. How to generate sticky ends? - (reply: 3)
1420. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1421. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1422. Relative RT-PCR, multiplate and calibration - (reply: 4)
1423. Trying to measure small levels of mRNA - (reply: 3)
1424. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1425. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
1426. Relative RT-PCR - (reply: 2)
1427. Relative real time RT PCR - (reply: 2)
1428. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1429. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1430. IQ5 and sybr/FAM - (reply: 3)
1431. a simple primer problem - (reply: 5)
1432. Failure to introduce mutations using Overlap PCR - (reply: 3)
1433. no or poor amplification - (reply: 1)
1434. Primers from 3'UTR - (reply: 2)
1435. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
1436. Designing primers with ESTs - (reply: 2)
1437. Ct variation/Ct limit/r˛ values/efficiency errors - (reply: 2)
1438. Probe manufacturers, who makes best probes? - Where do you get your probes and are you happy with them? (reply: 3)
1439. Real Time vs Traditional PCR results - (reply: 1)
1440. The problems to identify mouse genotype with PCR - (reply: 1)