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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1411. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
1412. argh more pcr headaches! - (reply: 6)
1413. Annealing temperature - (reply: 3)
1414. Replicate inconsistencies in qPCR - (reply: 6)
1415. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
1416. Chlamydia PCR cycling conditions - (reply: 1)
1417. On-unit screen or PC…which is better? - (reply: 12)
1418. Primers going "loopy" - (reply: 2)
1419. Biotinylated! - brief explanaion (reply: 2)
1420. what do i say?! - (reply: 2)
1421. Smearing on gel in PCR products - (reply: 5)
1422. 260/230 ratio: how low is good for running PCRs? - (reply: 2)
1423. introducing alanine mutations - (reply: 1)
1424. Hematin - calculation (reply: 1)
1425. How to generate sticky ends? - (reply: 3)
1426. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
1427. PROBLEM WITH MELTING CURVES - i realy need help (reply: 2)
1428. Relative RT-PCR, multiplate and calibration - (reply: 4)
1429. Trying to measure small levels of mRNA - (reply: 3)
1430. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
1431. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
1432. Relative RT-PCR - (reply: 2)
1433. Relative real time RT PCR - (reply: 2)
1434. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
1435. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
1436. IQ5 and sybr/FAM - (reply: 3)
1437. a simple primer problem - (reply: 5)
1438. Failure to introduce mutations using Overlap PCR - (reply: 3)
1439. no or poor amplification - (reply: 1)
1440. Primers from 3'UTR - (reply: 2)