Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1141. allele specific pcr - determining genotypes (reply: 2)
1142. Low 260/230 ratio in my RNA - (reply: 3)
1143. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
1144. Problem with primer efficiency analysis - (reply: 4)
1145. calibrator - what is this in qPCR (reply: 1)
1146. slan real time PCR system for validation of microarray results? - (reply: 1)
1147. PCR unusualband at ~230bps - PCR (reply: 1)
1148. DMSO or BSA for PCR - (reply: 6)
1149. primer design - (reply: 1)
1150. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
1151. Help me answer this curious student - Many questions on techniques (reply: 1)
1152. Tris Buffer for PCR reaction - why? (reply: 1)
1153. qPCR determination of phage numbers - slope difference for different phage clones (reply: 2)
1154. PCR a plasmid protein - (reply: 2)
1155. multiplex PCR - (reply: 4)
1156. Realtime PCR machine - (reply: 2)
1157. PCR set-up calculation nightmares. - (reply: 3)
1158. Primer desing - (reply: 2)
1159. minimum amount of cDNA? - molecular biology (reply: 6)
1160. Absolute quantification - (reply: 1)
1161. Fast and Furious: Multiplex PCR - Need expert's opinion and help---perhaps with a bit counseling (reply: 10)
1162. How to compare different expression levels between genes - (reply: 1)
1163. DNA contamination after RNA extraction, which kit works best? - (reply: 6)
1164. primer-blast from ncbi - (reply: 3)
1165. Page or HPLC primers - What is the difference (reply: 2)
1166. Standard curves for PCR efficiency. - (reply: 2)
1167. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
1168. Looking for help with my PCR! - (reply: 3)
1169. HKG validation - interplate normalisation (reply: 2)
1170. PCR without thermal cycler? - (reply: 9)