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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1141. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
1142. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
1143. colony PCR - (reply: 2)
1144. copy number variation - (reply: 3)
1145. How to deal with truncated mRNA or mRNA that has secondary structure - (reply: 2)
1146. Very weird gel - I cannot explain these results (reply: 11)
1147. well position effects (edge and corner) effects in qPCR - using Bio-Rad iCycler iQ (reply: 2)
1148. Cytosolic Housekeeping gene - (reply: 1)
1149. PCR reaction without template gives a product - (reply: 6)
1150. PCR wrong product size - (reply: 6)
1151. PCR very faint product - (reply: 1)
1152. proteinase k inhibition/inactivation - method... (reply: 2)
1153. What does melting temperature indicate and how to predict annealing temperature - (reply: 9)
1154. 4 question for real time PCR - (reply: 5)
1155. RNA quality - (reply: 3)
1156. Superscript III - No second strand synthesis? - (reply: 1)
1157. cDNA convertion to DNA - (reply: 4)
1158. PCR didn't work - suspect the enzyme (reply: 2)
1159. How to check RNA quality - (reply: 5)
1160. Colony PCR Specificity - (reply: 2)
1161. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
1162. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 9)
1163. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
1164. Primer Dilution Problem - D'oh! (reply: 2)
1165. Using PCR to create overhangs - (reply: 3)
1166. Site directed mutation - Changing D->K (reply: 2)
1167. Miner groove binder - urgent, help, what is it? (reply: 4)
1168. Problem with PCR on bacmids - (reply: 1)
1169. Copy number assay using Real time Pcr-SYBR green assay? - (reply: 3)
1170. FAM/HEX, lightcyler 480 - (reply: 1)