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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1141. qPCR Data analysis - qBasePlus and GenEx (reply: 1)
1142. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
1143. Degenerate primers PCR problem, Please help! - (reply: 2)
1144. degenerated Taqman probes? - (reply: 1)
1145. error message on qPCR mashine - (reply: 1)
1146. Hotstart PCR and unspecific Amplification - (reply: 3)
1147. Primer design help needed - (reply: 1)
1148. Primers no longer work - (reply: 1)
1149. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
1150. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
1151. colony PCR - (reply: 2)
1152. copy number variation - (reply: 3)
1153. How to deal with truncated mRNA or mRNA that has secondary structure - (reply: 2)
1154. Very weird gel - I cannot explain these results (reply: 11)
1155. well position effects (edge and corner) effects in qPCR - using Bio-Rad iCycler iQ (reply: 2)
1156. Cytosolic Housekeeping gene - (reply: 1)
1157. PCR reaction without template gives a product - (reply: 6)
1158. PCR wrong product size - (reply: 6)
1159. PCR very faint product - (reply: 1)
1160. proteinase k inhibition/inactivation - method... (reply: 2)
1161. What does melting temperature indicate and how to predict annealing temperature - (reply: 9)
1162. 4 question for real time PCR - (reply: 5)
1163. RNA quality - (reply: 3)
1164. Superscript III - No second strand synthesis? - (reply: 1)
1165. cDNA convertion to DNA - (reply: 4)
1166. PCR didn't work - suspect the enzyme (reply: 2)
1167. How to check RNA quality - (reply: 5)
1168. Colony PCR Specificity - (reply: 2)
1169. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
1170. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 9)