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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1141. very low expression of a sample vs standard curve efficiency - (reply: 1)
1142. Primer Reconstitution--does temperature matter? - (reply: 5)
1143. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
1144. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
1145. Oligonucleotide degradation - (reply: 1)
1146. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
1147. never get bands by pfu related enzymes - (reply: 2)
1148. Primer design for qPCR - (reply: 1)
1149. Primer-BLAST... - (reply: 4)
1150. reporting results of qPCR - (reply: 1)
1151. a question about 16s rRNA as reference in RT-qPCR - (reply: 1)
1152. PCR genomic DNA of high GC content - (reply: 6)
1153. question about qRT-PCR preparation - (reply: 1)
1154. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
1155. qPCR efficiency calculation - (reply: 1)
1156. qPCR on chIP product - (reply: 3)
1157. Ghost contamination in RT-PCR - Mystery that i can't solve. Help. (reply: 1)
1158. PCR--11kb fragment - (reply: 4)
1159. primer design tips - (reply: 3)
1160. GroEL from Wolbachia - GroEL from Wolbachia (reply: 2)
1161. PCR with Biotin Incorporation - (reply: 2)
1162. Anyone uses plasmid to make standard curves? how to make adjustment b/w plates - real time (reply: 4)
1163. pcr product - hello all (reply: 1)
1164. how to save sybr green reagents? - (reply: 3)
1165. amplification of GroEL from Wolbachia! - amplification of GroEL from Wolbachia! (reply: 2)
1166. Forgot to stop reverse transcription reaction - (reply: 4)
1167. PCR with long and complex primers - (reply: 2)
1168. Phospholylation of primers - (reply: 1)
1169. Re-using PCR plates? - (reply: 2)
1170. Touchdown PCR issues - (reply: 3)