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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1141. rna isolation manually - (reply: 2)
1142. phosphorylation of primers using PNK - (reply: 1)
1143. RT-PCR contamination issue - (reply: 3)
1144. What's the longest overhanging primer seq. you have used? - (reply: 2)
1145. Primer Concentration to lower Ct - (reply: 1)
1146. Primer Concentration Help - (reply: 4)
1147. Can normal sequencing services sequence >1kb product? - sequencing (reply: 1)
1148. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
1149. Primer Degradation - What exactly happens? (reply: 1)
1150. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
1151. PCR/DNA Extraction Problem - (reply: 5)
1152. Primers in 2 exons - (reply: 2)
1153. NTC appear in real time pcr - (reply: 6)
1154. RNA solutions - Calculating concentrations (reply: 9)
1155. PCR of AT-rich DNA - (reply: 1)
1156. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
1157. TBE Buffer? - (reply: 4)
1158. Overlapping PCR - really need help ! - (reply: 5)
1159. gel documentation sysytem - (reply: 7)
1160. internal standard - (reply: 5)
1161. Checking genomic contamination - different ways (reply: 4)
1162. Probe or Primer info for bovine AQP-1 - (reply: 6)
1163. PCR with Platinum Taq - product yield issues - (reply: 3)
1164. real time PCR - (reply: 7)
1165. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1166. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1167. Sample handling for RNA extraction - (reply: 1)
1168. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1169. Normalising RNA concentrations - Help (reply: 4)
1170. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)