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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1141. delta-delta-Ct method - what to do, when no expression in healthy control? (reply: 1)
1142. Trouble with annealing temperature - Trouble with annealing temperature (reply: 3)
1143. PCR Master mix - (reply: 2)
1144. Real Time PCR Primers and Probes - (reply: 2)
1145. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
1146. Dead genotyping PCR - I can't see s*** (reply: 5)
1147. Any one has light cycler 480 system operator's mannual? - Urgent!!!!!!!!!!!! (reply: 3)
1148. PCR product wrong size! Need help! - (reply: 9)
1149. pooling different pcr cycles? - (reply: 1)
1150. Amplification of concatenated linear ligated fragments - (reply: 4)
1151. Clinical Real-time PCR assay - (reply: 1)
1152. Alternative software to read .sds files? - (reply: 2)
1153. primer and promoter sequences - (reply: 1)
1154. Standard curve for real-time PCR - (reply: 2)
1155. delta Ct values - (reply: 1)
1156. Primer Tm differences/ PCR - (reply: 1)
1157. Interpretation of Ct values - (reply: 2)
1158. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
1159. Question about RT... - (reply: 1)
1160. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
1161. PCR Primer Dilution from F+R - (reply: 1)
1162. Plasmid standard problem - (reply: 2)
1163. primer reconstitution - primer reconstitution (reply: 4)
1164. Not-Quite-Nested PCR - (reply: 2)
1165. Primer design for insert amplification - Is this correct? (reply: 2)
1166. got smear on the PCR gel !!! - (reply: 4)
1167. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
1168. Troubleshooting reverse transcriptase-PCR - (reply: 3)
1169. "Unzipped" PCR band - (reply: 3)
1170. Review on new amplification techniques - (reply: 2)