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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1141. What’s wrong with my NTC - (reply: 2)
1142. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
1143. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
1144. PCR reamplification - (reply: 2)
1145. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
1146. question about pcr - (reply: 3)
1147. Standarization of HRM for methylation - (reply: 4)
1148. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
1149. How to construct a standard curve for real time PCR - (reply: 1)
1150. PCR with one primer - (reply: 1)
1151. cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
1152. First strand synthesis - (reply: 1)
1153. PCR contamination - (reply: 19)
1154. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1155. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1156. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1157. PCR not working - No amplification - (reply: 5)
1158. Quantification of PCR products - (reply: 1)
1159. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)
1160. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1161. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1162. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1163. Nested PCR question - (reply: 1)
1164. High Tm on primers - cannot get a product - (reply: 8)
1165. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1166. Help with Multiplex Nested PCR - (reply: 2)
1167. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1168. trembling dissociation curves in HRM assays - (reply: 3)
1169. Fluorescein storage? - (reply: 2)
1170. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)