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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1141. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1142. trembling dissociation curves in HRM assays - (reply: 3)
1143. Fluorescein storage? - (reply: 2)
1144. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1145. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1146. TELO2 & GAPDH - (reply: 2)
1147. PCR -No band formation - (reply: 1)
1148. 3' race - (reply: 1)
1149. PCR AMPLIFICATION - (reply: 1)
1150. internal control for genomic DNA - (reply: 1)
1151. qPCR Data Analysis - Software (reply: 3)
1152. Gene expression confusion - (reply: 5)
1153. real time pcr need advise - (reply: 2)
1154. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1155. ChIP-qPCR shift in melt curve - (reply: 4)
1156. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
1157. use of housekeeping gene in RT PCR chIP - (reply: 3)
1158. what is the order you follow in the run ??? - in order to make the contamination risk the least (reply: 3)
1159. DNA pooling for PCR - Saving money PCR (reply: 7)
1160. Real Time PCR Standard curves - How many are required??? (reply: 1)
1161. pcr-rflp - (reply: 2)
1162. PCR need some help - (reply: 4)
1163. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
1164. Left polymerase out overnight - Will it still work? (reply: 1)
1165. very low expression of a sample vs standard curve efficiency - (reply: 1)
1166. Primer Reconstitution--does temperature matter? - (reply: 5)
1167. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
1168. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
1169. Oligonucleotide degradation - (reply: 1)
1170. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)