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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1141. PCR contamination - (reply: 19)
1142. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1143. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1144. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1145. PCR not working - No amplification - (reply: 5)
1146. Quantification of PCR products - (reply: 1)
1147. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)
1148. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1149. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1150. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1151. Nested PCR question - (reply: 1)
1152. High Tm on primers - cannot get a product - (reply: 8)
1153. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1154. Help with Multiplex Nested PCR - (reply: 2)
1155. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1156. trembling dissociation curves in HRM assays - (reply: 3)
1157. Fluorescein storage? - (reply: 2)
1158. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1159. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1160. TELO2 & GAPDH - (reply: 2)
1161. PCR -No band formation - (reply: 1)
1162. 3' race - (reply: 1)
1163. PCR AMPLIFICATION - (reply: 1)
1164. internal control for genomic DNA - (reply: 1)
1165. qPCR Data Analysis - Software (reply: 3)
1166. Gene expression confusion - (reply: 5)
1167. real time pcr need advise - (reply: 2)
1168. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
1169. ChIP-qPCR shift in melt curve - (reply: 4)
1170. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)