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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
271. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
272. experiences with NuPCR from Illumina? - (reply: 1)
273. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
274. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
275. 100 ng RNA for cDNA synthesis - (reply: 4)
276. Inexplicable qPCR failure in single wells - (reply: 12)
277. negative control well 300 bp band - (reply: 3)
278. Reference gene for normalisation - for different growth rates - (reply: 3)
279. Strange amplification plots with high Ct variability - (reply: 1)
280. How big a role does mixing play in PCR - (reply: 1)
281. Melting curve is irregular for primer optimization - (reply: 5)
282. Designing primers for ABO blood groups - (reply: 1)
283. Methylight Results Analysis - (reply: 1)
284. GAPDH Ct value - (reply: 4)
285. dissociation curves - (reply: 1)
286. RT-PCR primer design - (reply: 7)
287. How to amplify very short PCR template - (reply: 4)
288. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
289. Is this primer okay? - (reply: 4)
290. Dilution calculation - (reply: 3)
291. Common causes for low RNA A260/230 ratios - (reply: 7)
292. Limit of detection for salmon gDNA? - (reply: 7)
293. Intensifying signal from positive control - (reply: 5)
294. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
295. analysing qpcr data and plotting standard curve - (reply: 1)
296. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
297. PCR product as standard curve template - (reply: 6)
298. TaqMan CN assay - control sample duplicated - (reply: 1)
299. qPCR - no amplification curve but suitable melting curve - (reply: 3)
300. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)