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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
271. using cell line to generate standard curve - (reply: 6)
272. PCR DNA Concentration - (reply: 1)
273. 3' RACE creates too big band doesn't leave well - (reply: 1)
274. RT-PCR product- no band - (reply: 4)
275. Normalization factor - (reply: 3)
276. Understanding RACE PCR - (reply: 1)
277. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
278. Dye for qPCR - (reply: 3)
279. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
280. Wierd Bands after PCR....Confused - (reply: 9)
281. PCR with Plasmid recovered from filter paper - (reply: 6)
282. PCR amplified product size - (reply: 5)
283. Opinion: What fold change is actually considered meaningful - (reply: 2)
284. Buffers RNase decontamination - (reply: 1)
285. the storage time for primers - (reply: 9)
286. Annealing temperature for PCR - (reply: 8)
287. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
288. Nested PCR - (reply: 2)
289. random primers or oligodT - (reply: 4)
290. Confused about normalization - (reply: 2)
291. To design or use published primers? - (reply: 4)
292. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
293. PCR primer usage (Clonning & cDNA) - (reply: 2)
294. Taq polymerase - (reply: 7)
295. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
296. Which High-Fidelity polymerase is better? - (reply: 2)
297. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
298. reference gene for qPCR in E.coli - (reply: 1)
299. Problem amplifying viral gene - (reply: 5)
300. The same CT with different Quantity, why? - (reply: 8)