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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
271. hybridization probe - (reply: 1)
272. How to get rid of bands in PCR negative control - (reply: 10)
273. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
274. standard curve using genomic dna - (reply: 1)
275. PCR problem - (reply: 2)
276. defining ratio of alternative spliced gene with qPCR - (reply: 14)
277. Poor efficiency standard curve - (reply: 2)
278. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
279. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
280. PCR gene specific amplification problem - (reply: 3)
281. Failure SYBRGREEN PCR - (reply: 4)
282. Pfaffl Method for relative - (reply: 4)
283. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
284. experiences with NuPCR from Illumina? - (reply: 1)
285. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
286. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
287. 100 ng RNA for cDNA synthesis - (reply: 4)
288. Inexplicable qPCR failure in single wells - (reply: 12)
289. negative control well 300 bp band - (reply: 3)
290. Reference gene for normalisation - for different growth rates - (reply: 3)
291. Strange amplification plots with high Ct variability - (reply: 1)
292. How big a role does mixing play in PCR - (reply: 1)
293. Melting curve is irregular for primer optimization - (reply: 5)
294. Designing primers for ABO blood groups - (reply: 1)
295. Methylight Results Analysis - (reply: 1)
296. GAPDH Ct value - (reply: 4)
297. dissociation curves - (reply: 1)
298. RT-PCR primer design - (reply: 7)
299. How to amplify very short PCR template - (reply: 4)
300. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)