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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
271. High Ct values in ref gene as well as target gene - (reply: 3)
272. GADPH does not express - (reply: 1)
273. qPCR reference gene testing help - (reply: 4)
274. protocol to relieve melanin inhibition of PCR - (reply: 4)
275. No bands despite different primers and conditions and TAqs - (reply: 8)
276. Multiplex TaqMan-like Assay PCR Efficiency - (reply: 3)
277. Wrong PCR product - (reply: 2)
278. Primer design - (reply: 5)
279. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
280. Quantitative RT-PCR statistics help - (reply: 1)
281. PCR Primer trouble - (reply: 2)
282. I don't find housekeeping genes: what should I do? - (reply: 6)
283. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
284. excess rna sample affect qpcr? - (reply: 2)
285. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
286. finding the corrosponding primers - (reply: 2)
287. Gene sequence for Real time PCR - (reply: 2)
288. qPCR result of pooled samples - (reply: 1)
289. transcript variant X - (reply: 5)
290. RNA extraction for RT-PCR - (reply: 3)
291. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
292. problems with gDNA doing real time PCR in yeast - (reply: 2)
293. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
294. hybridization probe - (reply: 1)
295. How to get rid of bands in PCR negative control - (reply: 10)
296. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
297. standard curve using genomic dna - (reply: 1)
298. PCR problem - (reply: 2)
299. defining ratio of alternative spliced gene with qPCR - (reply: 14)
300. Poor efficiency standard curve - (reply: 2)