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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
271. Template DNA for PCR- Concrentration or Volume?? - (reply: 1)
272. RNA extraction - (reply: 3)
273. Normalization of Ct of interest to a reference Ct - (reply: 3)
274. urgent: need help with qPCR statistics - (reply: 2)
275. A question for Reverse transcription experts - (reply: 5)
276. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
277. PCR amplification with new restriction sites troubleshooting - (reply: 2)
278. using cell line to generate standard curve - (reply: 6)
279. PCR DNA Concentration - (reply: 1)
280. 3' RACE creates too big band doesn't leave well - (reply: 1)
281. RT-PCR product- no band - (reply: 4)
282. Normalization factor - (reply: 3)
283. Understanding RACE PCR - (reply: 1)
284. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
285. Dye for qPCR - (reply: 3)
286. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
287. Wierd Bands after PCR....Confused - (reply: 9)
288. PCR with Plasmid recovered from filter paper - (reply: 6)
289. PCR amplified product size - (reply: 5)
290. Opinion: What fold change is actually considered meaningful - (reply: 2)
291. Buffers RNase decontamination - (reply: 1)
292. the storage time for primers - (reply: 9)
293. Annealing temperature for PCR - (reply: 8)
294. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
295. Nested PCR - (reply: 2)
296. random primers or oligodT - (reply: 4)
297. Confused about normalization - (reply: 2)
298. To design or use published primers? - (reply: 4)
299. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
300. PCR primer usage (Clonning & cDNA) - (reply: 2)