Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
271. TaqMan CN assay - control sample duplicated - (reply: 1)
272. qPCR - no amplification curve but suitable melting curve - (reply: 3)
273. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
274. Basic question about qPCR and endogenous RNA control. - (reply: 4)
275. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
276. something very basic, why called "event-specific detection" - (reply: 1)
277. skip 65 degrees protocol of Reverse transcription - (reply: 4)
278. Mystery in my PCR - (reply: 5)
279. Whole genome amplification from cDNA? - (reply: 4)
280. What do you use to check primer secondary structure? - (reply: 3)
281. CT of qPCR - (reply: 3)
282. Need help with my real time RT-PCR Plate Set up - (reply: 8)
283. PCR product as template for in vitro transcription - (reply: 1)
284. Sybr Green vs Taqman -- a practical approach - (reply: 2)
285. Problem with 3 step PCR - (reply: 3)
286. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
287. ARMS-PCR - (reply: 1)
288. Scaling up PCR to get more DNA - (reply: 5)
289. RNA concentration suddenly drops - (reply: 3)
290. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
291. PCR to get 10kbp product - (reply: 4)
292. site-directed mutagenesis primer Tm - (reply: 4)
293. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
294. RNA concentration to start RT reaction - (reply: 1)
295. Random vs oligo primer in preamplification RT - (reply: 1)
296. Smart Cycler II software and Absolute quantification help - (reply: 1)
297. cDNA amount in qPCR - (reply: 2)
298. mRNA integrity for qPCR - (reply: 6)
299. Strange control contamination in cDNA synthesis - (reply: 2)
300. how does polymerase stop at the required length - (reply: 1)