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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1171. RNA solutions - Calculating concentrations (reply: 9)
1172. PCR of AT-rich DNA - (reply: 1)
1173. NTP mix (with dUTP instead of dTTP) in sybr green mix - (reply: 1)
1174. TBE Buffer? - (reply: 4)
1175. Overlapping PCR - really need help ! - (reply: 5)
1176. gel documentation sysytem - (reply: 7)
1177. internal standard - (reply: 5)
1178. Checking genomic contamination - different ways (reply: 4)
1179. Probe or Primer info for bovine AQP-1 - (reply: 6)
1180. PCR with Platinum Taq - product yield issues - (reply: 3)
1181. real time PCR - (reply: 7)
1182. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1183. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1184. Sample handling for RNA extraction - (reply: 1)
1185. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1186. Normalising RNA concentrations - Help (reply: 4)
1187. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
1188. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
1189. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
1190. Having abnormal dissociation curve when performing qPCR - (reply: 3)
1191. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
1192. Please help me if you could - (reply: 5)
1193. Tm caculation for different PCRs?? - new, confused, and frustrated beginner (reply: 3)
1194. absolute quantification - (reply: 4)
1195. The Case of a Missing Band: PCR Issue - (reply: 4)
1196. Log 10 fold serial dilutions - (reply: 2)
1197. paranormal qpcr amplification activity - melting curves (reply: 3)
1198. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
1199. PCR template concentration - (reply: 6)
1200. PCR efficiency calculated by Linreg - (reply: 3)