Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1171. Checking genomic contamination - different ways (reply: 4)
1172. Probe or Primer info for bovine AQP-1 - (reply: 6)
1173. PCR with Platinum Taq - product yield issues - (reply: 3)
1174. real time PCR - (reply: 7)
1175. very high and inconsistent Cts for the house keeping gene - (reply: 4)
1176. Standard curves, are they necessary every time we run an experiment? - (reply: 6)
1177. Sample handling for RNA extraction - (reply: 1)
1178. beginners guide to qPCR for differences in gene expression - Help (reply: 4)
1179. Normalising RNA concentrations - Help (reply: 4)
1180. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
1181. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
1182. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
1183. Having abnormal dissociation curve when performing qPCR - (reply: 3)
1184. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
1185. Please help me if you could - (reply: 5)
1186. Tm caculation for different PCRs?? - new, confused, and frustrated beginner (reply: 3)
1187. absolute quantification - (reply: 4)
1188. The Case of a Missing Band: PCR Issue - (reply: 4)
1189. Log 10 fold serial dilutions - (reply: 2)
1190. paranormal qpcr amplification activity - melting curves (reply: 3)
1191. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
1192. PCR template concentration - (reply: 6)
1193. PCR efficiency calculated by Linreg - (reply: 3)
1194. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
1195. Stability of genomic DNA in 1x PBS - (reply: 2)
1196. primer design by generunner DG - (reply: 1)
1197. Calculation of Tm of amplicon when using SYBR Green - (reply: 3)
1198. degenerate pcr - (reply: 1)
1199. DNA degradation - (reply: 1)
1200. DNase 1 treratment of RNA - (reply: 11)