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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1171. Hotstart PCR and unspecific Amplification - (reply: 3)
1172. Primer design help needed - (reply: 1)
1173. Primers no longer work - (reply: 1)
1174. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
1175. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
1176. colony PCR - (reply: 2)
1177. copy number variation - (reply: 3)
1178. How to deal with truncated mRNA or mRNA that has secondary structure - (reply: 2)
1179. Very weird gel - I cannot explain these results (reply: 11)
1180. well position effects (edge and corner) effects in qPCR - using Bio-Rad iCycler iQ (reply: 2)
1181. Cytosolic Housekeeping gene - (reply: 1)
1182. PCR reaction without template gives a product - (reply: 6)
1183. PCR wrong product size - (reply: 6)
1184. PCR very faint product - (reply: 1)
1185. proteinase k inhibition/inactivation - method... (reply: 2)
1186. What does melting temperature indicate and how to predict annealing temperature - (reply: 9)
1187. 4 question for real time PCR - (reply: 5)
1188. RNA quality - (reply: 3)
1189. Superscript III - No second strand synthesis? - (reply: 1)
1190. cDNA convertion to DNA - (reply: 4)
1191. PCR didn't work - suspect the enzyme (reply: 2)
1192. How to check RNA quality - (reply: 5)
1193. Colony PCR Specificity - (reply: 2)
1194. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
1195. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 9)
1196. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
1197. Primer Dilution Problem - D'oh! (reply: 2)
1198. Using PCR to create overhangs - (reply: 3)
1199. Site directed mutation - Changing D->K (reply: 2)
1200. Miner groove binder - urgent, help, what is it? (reply: 4)