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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1171. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
1172. use of housekeeping gene in RT PCR chIP - (reply: 3)
1173. what is the order you follow in the run ??? - in order to make the contamination risk the least (reply: 3)
1174. DNA pooling for PCR - Saving money PCR (reply: 7)
1175. Real Time PCR Standard curves - How many are required??? (reply: 1)
1176. pcr-rflp - (reply: 2)
1177. PCR need some help - (reply: 4)
1178. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
1179. Left polymerase out overnight - Will it still work? (reply: 1)
1180. very low expression of a sample vs standard curve efficiency - (reply: 1)
1181. Primer Reconstitution--does temperature matter? - (reply: 5)
1182. TaqMan Gene Expression Assays - Low expression/ Ct values (reply: 2)
1183. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
1184. Oligonucleotide degradation - (reply: 1)
1185. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
1186. never get bands by pfu related enzymes - (reply: 2)
1187. Primer design for qPCR - (reply: 1)
1188. Primer-BLAST... - (reply: 4)
1189. reporting results of qPCR - (reply: 1)
1190. a question about 16s rRNA as reference in RT-qPCR - (reply: 1)
1191. PCR genomic DNA of high GC content - (reply: 6)
1192. question about qRT-PCR preparation - (reply: 1)
1193. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
1194. qPCR efficiency calculation - (reply: 1)
1195. qPCR on chIP product - (reply: 3)
1196. Ghost contamination in RT-PCR - Mystery that i can't solve. Help. (reply: 1)
1197. PCR--11kb fragment - (reply: 4)
1198. primer design tips - (reply: 3)
1199. GroEL from Wolbachia - GroEL from Wolbachia (reply: 2)
1200. PCR with Biotin Incorporation - (reply: 2)