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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1171. What does melting temperature indicate and how to predict annealing temperature - (reply: 9)
1172. 4 question for real time PCR - (reply: 5)
1173. RNA quality - (reply: 3)
1174. Superscript III - No second strand synthesis? - (reply: 1)
1175. cDNA convertion to DNA - (reply: 4)
1176. PCR didn't work - suspect the enzyme (reply: 2)
1177. How to check RNA quality - (reply: 5)
1178. Colony PCR Specificity - (reply: 2)
1179. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
1180. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 9)
1181. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
1182. Primer Dilution Problem - D'oh! (reply: 2)
1183. Using PCR to create overhangs - (reply: 3)
1184. Site directed mutation - Changing D->K (reply: 2)
1185. Miner groove binder - urgent, help, what is it? (reply: 4)
1186. Problem with PCR on bacmids - (reply: 1)
1187. Copy number assay using Real time Pcr-SYBR green assay? - (reply: 3)
1188. FAM/HEX, lightcyler 480 - (reply: 1)
1189. Running real time pcr product on gel?.. - (reply: 4)
1190. help with ct of reference gene - (reply: 1)
1191. PCR mutagenesis of plasmid - (reply: 5)
1192. Primer designed for ARMS PCR - (reply: 2)
1193. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
1194. methylation specific PCR - Primers - (reply: 1)
1195. bacterial identification using real time PCR - (reply: 1)
1196. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
1197. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
1198. Problem with multiplex PCR - Troubleshooting (reply: 4)
1199. Questions about ChIP protocols - (reply: 1)
1200. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)