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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1171. error message on qPCR mashine - (reply: 1)
1172. Hotstart PCR and unspecific Amplification - (reply: 3)
1173. Primer design help needed - (reply: 1)
1174. Primers no longer work - (reply: 1)
1175. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
1176. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
1177. colony PCR - (reply: 2)
1178. copy number variation - (reply: 3)
1179. How to deal with truncated mRNA or mRNA that has secondary structure - (reply: 2)
1180. Very weird gel - I cannot explain these results (reply: 11)
1181. well position effects (edge and corner) effects in qPCR - using Bio-Rad iCycler iQ (reply: 2)
1182. Cytosolic Housekeeping gene - (reply: 1)
1183. PCR reaction without template gives a product - (reply: 6)
1184. PCR wrong product size - (reply: 6)
1185. PCR very faint product - (reply: 1)
1186. proteinase k inhibition/inactivation - method... (reply: 2)
1187. What does melting temperature indicate and how to predict annealing temperature - (reply: 9)
1188. 4 question for real time PCR - (reply: 5)
1189. RNA quality - (reply: 3)
1190. Superscript III - No second strand synthesis? - (reply: 1)
1191. cDNA convertion to DNA - (reply: 4)
1192. PCR didn't work - suspect the enzyme (reply: 2)
1193. How to check RNA quality - (reply: 5)
1194. Colony PCR Specificity - (reply: 2)
1195. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
1196. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 9)
1197. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
1198. Primer Dilution Problem - D'oh! (reply: 2)
1199. Using PCR to create overhangs - (reply: 3)
1200. Site directed mutation - Changing D->K (reply: 2)