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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1171. PCR reamplification - (reply: 2)
1172. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
1173. question about pcr - (reply: 3)
1174. Standarization of HRM for methylation - (reply: 4)
1175. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
1176. How to construct a standard curve for real time PCR - (reply: 1)
1177. PCR with one primer - (reply: 1)
1178. cDNA prepared with varying amounts of RNA - is the amount of cDNA produced proportional to the input RNA? (reply: 3)
1179. First strand synthesis - (reply: 1)
1180. PCR contamination - (reply: 19)
1181. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
1182. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
1183. How to do HRM curve analyses with SYBR Green Dye? - How to do HRM curve analyses with SYBR Green Dye using ABI 7500 RT-PC? (reply: 5)
1184. PCR not working - No amplification - (reply: 5)
1185. Quantification of PCR products - (reply: 1)
1186. Comment: FYI be careful which program you use to analyze data from Bio-Rad therm - and be careful of the advice Bio-Rad offers (reply: 1)
1187. homemade mastermix for qPCR - any reason not to make a lot and freeze - (reply: 9)
1188. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
1189. qPCR for telomere length measurement - efficiency issues - complicated monochrome multiplex assay with SYBR Green (reply: 40)
1190. Nested PCR question - (reply: 1)
1191. High Tm on primers - cannot get a product - (reply: 8)
1192. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
1193. Help with Multiplex Nested PCR - (reply: 2)
1194. Relative quantification analysis Ct values & 2-ΔΔCT method - samples which do not amplify (reply: 5)
1195. trembling dissociation curves in HRM assays - (reply: 3)
1196. Fluorescein storage? - (reply: 2)
1197. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
1198. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
1199. TELO2 & GAPDH - (reply: 2)
1200. PCR -No band formation - (reply: 1)