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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1171. qPCR Data analysis - qBasePlus and GenEx (reply: 1)
1172. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
1173. Degenerate primers PCR problem, Please help! - (reply: 2)
1174. degenerated Taqman probes? - (reply: 1)
1175. error message on qPCR mashine - (reply: 1)
1176. Hotstart PCR and unspecific Amplification - (reply: 3)
1177. Primer design help needed - (reply: 1)
1178. Primers no longer work - (reply: 1)
1179. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
1180. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
1181. colony PCR - (reply: 2)
1182. copy number variation - (reply: 3)
1183. How to deal with truncated mRNA or mRNA that has secondary structure - (reply: 2)
1184. Very weird gel - I cannot explain these results (reply: 11)
1185. well position effects (edge and corner) effects in qPCR - using Bio-Rad iCycler iQ (reply: 2)
1186. Cytosolic Housekeeping gene - (reply: 1)
1187. PCR reaction without template gives a product - (reply: 6)
1188. PCR wrong product size - (reply: 6)
1189. PCR very faint product - (reply: 1)
1190. proteinase k inhibition/inactivation - method... (reply: 2)
1191. What does melting temperature indicate and how to predict annealing temperature - (reply: 9)
1192. 4 question for real time PCR - (reply: 5)
1193. RNA quality - (reply: 3)
1194. Superscript III - No second strand synthesis? - (reply: 1)
1195. cDNA convertion to DNA - (reply: 4)
1196. PCR didn't work - suspect the enzyme (reply: 2)
1197. How to check RNA quality - (reply: 5)
1198. Colony PCR Specificity - (reply: 2)
1199. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
1200. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 9)