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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
1171. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
1172. colony PCR - (reply: 2)
1173. copy number variation - (reply: 3)
1174. How to deal with truncated mRNA or mRNA that has secondary structure - (reply: 2)
1175. Very weird gel - I cannot explain these results (reply: 11)
1176. well position effects (edge and corner) effects in qPCR - using Bio-Rad iCycler iQ (reply: 2)
1177. Cytosolic Housekeeping gene - (reply: 1)
1178. PCR reaction without template gives a product - (reply: 6)
1179. PCR wrong product size - (reply: 6)
1180. PCR very faint product - (reply: 1)
1181. proteinase k inhibition/inactivation - method... (reply: 2)
1182. What does melting temperature indicate and how to predict annealing temperature - (reply: 9)
1183. 4 question for real time PCR - (reply: 5)
1184. RNA quality - (reply: 3)
1185. Superscript III - No second strand synthesis? - (reply: 1)
1186. cDNA convertion to DNA - (reply: 4)
1187. PCR didn't work - suspect the enzyme (reply: 2)
1188. How to check RNA quality - (reply: 5)
1189. Colony PCR Specificity - (reply: 2)
1190. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
1191. Reverse Transcriptase @ Room temperature for 48 hours - (reply: 9)
1192. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
1193. Primer Dilution Problem - D'oh! (reply: 2)
1194. Using PCR to create overhangs - (reply: 3)
1195. Site directed mutation - Changing D->K (reply: 2)
1196. Miner groove binder - urgent, help, what is it? (reply: 4)
1197. Problem with PCR on bacmids - (reply: 1)
1198. Copy number assay using Real time Pcr-SYBR green assay? - (reply: 3)
1199. FAM/HEX, lightcyler 480 - (reply: 1)
1200. Running real time pcr product on gel?.. - (reply: 4)