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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. Limit of detection for salmon gDNA? - (reply: 7)
332. Intensifying signal from positive control - (reply: 5)
333. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
334. analysing qpcr data and plotting standard curve - (reply: 1)
335. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
336. PCR product as standard curve template - (reply: 6)
337. TaqMan CN assay - control sample duplicated - (reply: 1)
338. qPCR - no amplification curve but suitable melting curve - (reply: 3)
339. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
340. Basic question about qPCR and endogenous RNA control. - (reply: 4)
341. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
342. something very basic, why called "event-specific detection" - (reply: 1)
343. skip 65 degrees protocol of Reverse transcription - (reply: 4)
344. Mystery in my PCR - (reply: 5)
345. Whole genome amplification from cDNA? - (reply: 4)
346. What do you use to check primer secondary structure? - (reply: 3)
347. CT of qPCR - (reply: 3)
348. Need help with my real time RT-PCR Plate Set up - (reply: 8)
349. PCR product as template for in vitro transcription - (reply: 1)
350. Sybr Green vs Taqman -- a practical approach - (reply: 2)
351. Problem with 3 step PCR - (reply: 3)
352. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
353. ARMS-PCR - (reply: 1)
354. Scaling up PCR to get more DNA - (reply: 5)
355. RNA concentration suddenly drops - (reply: 3)
356. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
357. PCR to get 10kbp product - (reply: 4)
358. site-directed mutagenesis primer Tm - (reply: 4)
359. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
360. RNA concentration to start RT reaction - (reply: 1)