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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. Adding tag using overlapping PCR - (reply: 2)
332. Can you buy SYBR Green dye that is not in a master mix? - (reply: 3)
333. Design primer from incomplete sequence... - (reply: 2)
334. Long term storage for RNA extracted from pancreas - (reply: 3)
335. How to perform colony PCR - (reply: 1)
336. qPCR from HEK cells transfected with Plasmid - (reply: 4)
337. DNA size band increased after gel electrophoresis with Quaigen fit - (reply: 4)
338. Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (reply: 1)
339. Negative Control Looks Like the Rest - (reply: 11)
340. Why RNase H treatment after cDNA synthesis - (reply: 2)
341. RNA isolation from Sesame seed - (reply: 3)
342. qPCR with Genomic DNA - (reply: 1)
343. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
344. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
345. Homogenize pancreas for RNA extraction - (reply: 3)
346. Point mutagenesis..... - (reply: 6)
347. No bands in PCR after DNase treatment - (reply: 4)
348. Overlap PCR problem - (reply: 5)
349. PCR product purification - (reply: 4)
350. Overlapping sequence PCR primers - (reply: 1)
351. acceptable melt curve? - (reply: 1)
352. Calibrator sample - (reply: 4)
353. Ct value very high. What can I do to improve this ct? - (reply: 4)
354. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
355. Amount of RNA (ng) for good ct values. - (reply: 3)
356. Normalizing qPCR data - (reply: 1)
357. doing PCR using PCR product , help plz !! - (reply: 5)
358. High GC content RT-qPCR - please help - (reply: 3)
359. Electrophoresis problem , please help pals ! - (reply: 8)
360. Calculating Standard Errors and Normalising to Transgene - (reply: 1)