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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. PCR gene specific amplification problem - (reply: 3)
332. Failure SYBRGREEN PCR - (reply: 4)
333. Pfaffl Method for relative - (reply: 4)
334. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
335. experiences with NuPCR from Illumina? - (reply: 1)
336. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
337. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
338. 100 ng RNA for cDNA synthesis - (reply: 4)
339. Inexplicable qPCR failure in single wells - (reply: 12)
340. negative control well 300 bp band - (reply: 3)
341. Reference gene for normalisation - for different growth rates - (reply: 3)
342. Strange amplification plots with high Ct variability - (reply: 1)
343. How big a role does mixing play in PCR - (reply: 1)
344. Melting curve is irregular for primer optimization - (reply: 5)
345. Designing primers for ABO blood groups - (reply: 1)
346. Methylight Results Analysis - (reply: 1)
347. GAPDH Ct value - (reply: 4)
348. dissociation curves - (reply: 1)
349. RT-PCR primer design - (reply: 7)
350. How to amplify very short PCR template - (reply: 4)
351. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
352. Is this primer okay? - (reply: 4)
353. Dilution calculation - (reply: 3)
354. Common causes for low RNA A260/230 ratios - (reply: 7)
355. Limit of detection for salmon gDNA? - (reply: 7)
356. Intensifying signal from positive control - (reply: 5)
357. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
358. analysing qpcr data and plotting standard curve - (reply: 1)
359. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
360. PCR product as standard curve template - (reply: 6)