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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. PCR amplification with new restriction sites troubleshooting - (reply: 2)
332. using cell line to generate standard curve - (reply: 6)
333. PCR DNA Concentration - (reply: 1)
334. 3' RACE creates too big band doesn't leave well - (reply: 1)
335. RT-PCR product- no band - (reply: 4)
336. Normalization factor - (reply: 3)
337. Understanding RACE PCR - (reply: 1)
338. SYBR Select Master Mix for CFX from Invitrogen - Storage - (reply: 4)
339. Dye for qPCR - (reply: 3)
340. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
341. Wierd Bands after PCR....Confused - (reply: 9)
342. PCR with Plasmid recovered from filter paper - (reply: 6)
343. PCR amplified product size - (reply: 5)
344. Opinion: What fold change is actually considered meaningful - (reply: 2)
345. Buffers RNase decontamination - (reply: 1)
346. the storage time for primers - (reply: 9)
347. Annealing temperature for PCR - (reply: 8)
348. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
349. Nested PCR - (reply: 2)
350. random primers or oligodT - (reply: 4)
351. Confused about normalization - (reply: 2)
352. To design or use published primers? - (reply: 4)
353. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
354. PCR primer usage (Clonning & cDNA) - (reply: 2)
355. Taq polymerase - (reply: 7)
356. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
357. Which High-Fidelity polymerase is better? - (reply: 2)
358. How 18s gene can be amplified when I use oligo dt for cDNA synthesis - (reply: 4)
359. reference gene for qPCR in E.coli - (reply: 2)
360. Problem amplifying viral gene - (reply: 5)