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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. analysing qpcr data and plotting standard curve - (reply: 1)
332. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
333. PCR product as standard curve template - (reply: 6)
334. TaqMan CN assay - control sample duplicated - (reply: 1)
335. qPCR - no amplification curve but suitable melting curve - (reply: 3)
336. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
337. Basic question about qPCR and endogenous RNA control. - (reply: 4)
338. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
339. something very basic, why called "event-specific detection" - (reply: 1)
340. skip 65 degrees protocol of Reverse transcription - (reply: 4)
341. Mystery in my PCR - (reply: 5)
342. Whole genome amplification from cDNA? - (reply: 4)
343. What do you use to check primer secondary structure? - (reply: 3)
344. CT of qPCR - (reply: 3)
345. Need help with my real time RT-PCR Plate Set up - (reply: 8)
346. PCR product as template for in vitro transcription - (reply: 1)
347. Sybr Green vs Taqman -- a practical approach - (reply: 2)
348. Problem with 3 step PCR - (reply: 3)
349. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
350. ARMS-PCR - (reply: 1)
351. Scaling up PCR to get more DNA - (reply: 5)
352. RNA concentration suddenly drops - (reply: 3)
353. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
354. PCR to get 10kbp product - (reply: 4)
355. site-directed mutagenesis primer Tm - (reply: 4)
356. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
357. RNA concentration to start RT reaction - (reply: 1)
358. Random vs oligo primer in preamplification RT - (reply: 1)
359. Smart Cycler II software and Absolute quantification help - (reply: 1)
360. cDNA amount in qPCR - (reply: 2)