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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. Intensifying signal from positive control - (reply: 5)
332. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
333. analysing qpcr data and plotting standard curve - (reply: 1)
334. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
335. PCR product as standard curve template - (reply: 6)
336. TaqMan CN assay - control sample duplicated - (reply: 1)
337. qPCR - no amplification curve but suitable melting curve - (reply: 3)
338. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
339. Basic question about qPCR and endogenous RNA control. - (reply: 4)
340. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
341. something very basic, why called "event-specific detection" - (reply: 1)
342. skip 65 degrees protocol of Reverse transcription - (reply: 4)
343. Mystery in my PCR - (reply: 5)
344. Whole genome amplification from cDNA? - (reply: 4)
345. What do you use to check primer secondary structure? - (reply: 3)
346. CT of qPCR - (reply: 3)
347. Need help with my real time RT-PCR Plate Set up - (reply: 8)
348. PCR product as template for in vitro transcription - (reply: 1)
349. Sybr Green vs Taqman -- a practical approach - (reply: 2)
350. Problem with 3 step PCR - (reply: 3)
351. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
352. ARMS-PCR - (reply: 1)
353. Scaling up PCR to get more DNA - (reply: 5)
354. RNA concentration suddenly drops - (reply: 3)
355. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
356. PCR to get 10kbp product - (reply: 4)
357. site-directed mutagenesis primer Tm - (reply: 4)
358. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
359. RNA concentration to start RT reaction - (reply: 1)
360. Random vs oligo primer in preamplification RT - (reply: 1)