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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. negative control well 300 bp band - (reply: 3)
332. Reference gene for normalisation - for different growth rates - (reply: 3)
333. Strange amplification plots with high Ct variability - (reply: 1)
334. How big a role does mixing play in PCR - (reply: 1)
335. Melting curve is irregular for primer optimization - (reply: 5)
336. Designing primers for ABO blood groups - (reply: 1)
337. Methylight Results Analysis - (reply: 1)
338. GAPDH Ct value - (reply: 4)
339. dissociation curves - (reply: 1)
340. RT-PCR primer design - (reply: 7)
341. How to amplify very short PCR template - (reply: 4)
342. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
343. Is this primer okay? - (reply: 4)
344. Dilution calculation - (reply: 3)
345. Common causes for low RNA A260/230 ratios - (reply: 7)
346. Limit of detection for salmon gDNA? - (reply: 7)
347. Intensifying signal from positive control - (reply: 5)
348. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
349. analysing qpcr data and plotting standard curve - (reply: 1)
350. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
351. PCR product as standard curve template - (reply: 6)
352. TaqMan CN assay - control sample duplicated - (reply: 1)
353. qPCR - no amplification curve but suitable melting curve - (reply: 3)
354. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
355. Basic question about qPCR and endogenous RNA control. - (reply: 4)
356. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
357. something very basic, why called "event-specific detection" - (reply: 1)
358. skip 65 degrees protocol of Reverse transcription - (reply: 4)
359. Mystery in my PCR - (reply: 5)
360. Whole genome amplification from cDNA? - (reply: 4)