Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
332. hybridization probe - (reply: 1)
333. How to get rid of bands in PCR negative control - (reply: 10)
334. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
335. standard curve using genomic dna - (reply: 1)
336. PCR problem - (reply: 2)
337. defining ratio of alternative spliced gene with qPCR - (reply: 14)
338. Poor efficiency standard curve - (reply: 2)
339. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
340. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
341. PCR gene specific amplification problem - (reply: 3)
342. Failure SYBRGREEN PCR - (reply: 4)
343. Pfaffl Method for relative - (reply: 4)
344. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
345. experiences with NuPCR from Illumina? - (reply: 1)
346. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
347. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
348. 100 ng RNA for cDNA synthesis - (reply: 4)
349. Inexplicable qPCR failure in single wells - (reply: 12)
350. negative control well 300 bp band - (reply: 3)
351. Reference gene for normalisation - for different growth rates - (reply: 3)
352. Strange amplification plots with high Ct variability - (reply: 1)
353. How big a role does mixing play in PCR - (reply: 1)
354. Melting curve is irregular for primer optimization - (reply: 5)
355. Designing primers for ABO blood groups - (reply: 1)
356. Methylight Results Analysis - (reply: 1)
357. GAPDH Ct value - (reply: 4)
358. dissociation curves - (reply: 1)
359. RT-PCR primer design - (reply: 7)
360. How to amplify very short PCR template - (reply: 4)