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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. Methylight Results Analysis - (reply: 1)
332. GAPDH Ct value - (reply: 4)
333. dissociation curves - (reply: 1)
334. RT-PCR primer design - (reply: 7)
335. How to amplify very short PCR template - (reply: 4)
336. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
337. Is this primer okay? - (reply: 4)
338. Dilution calculation - (reply: 3)
339. Common causes for low RNA A260/230 ratios - (reply: 7)
340. Limit of detection for salmon gDNA? - (reply: 7)
341. Intensifying signal from positive control - (reply: 5)
342. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
343. analysing qpcr data and plotting standard curve - (reply: 1)
344. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
345. PCR product as standard curve template - (reply: 6)
346. TaqMan CN assay - control sample duplicated - (reply: 1)
347. qPCR - no amplification curve but suitable melting curve - (reply: 3)
348. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
349. Basic question about qPCR and endogenous RNA control. - (reply: 4)
350. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
351. something very basic, why called "event-specific detection" - (reply: 1)
352. skip 65 degrees protocol of Reverse transcription - (reply: 4)
353. Mystery in my PCR - (reply: 5)
354. Whole genome amplification from cDNA? - (reply: 4)
355. What do you use to check primer secondary structure? - (reply: 3)
356. CT of qPCR - (reply: 3)
357. Need help with my real time RT-PCR Plate Set up - (reply: 8)
358. PCR product as template for in vitro transcription - (reply: 1)
359. Sybr Green vs Taqman -- a practical approach - (reply: 2)
360. Problem with 3 step PCR - (reply: 3)