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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. RT-PCR primer design - (reply: 7)
332. How to amplify very short PCR template - (reply: 4)
333. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
334. Is this primer okay? - (reply: 4)
335. Dilution calculation - (reply: 3)
336. Common causes for low RNA A260/230 ratios - (reply: 7)
337. Limit of detection for salmon gDNA? - (reply: 7)
338. Intensifying signal from positive control - (reply: 5)
339. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
340. analysing qpcr data and plotting standard curve - (reply: 1)
341. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
342. PCR product as standard curve template - (reply: 6)
343. TaqMan CN assay - control sample duplicated - (reply: 1)
344. qPCR - no amplification curve but suitable melting curve - (reply: 3)
345. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
346. Basic question about qPCR and endogenous RNA control. - (reply: 4)
347. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
348. something very basic, why called "event-specific detection" - (reply: 1)
349. skip 65 degrees protocol of Reverse transcription - (reply: 4)
350. Mystery in my PCR - (reply: 5)
351. Whole genome amplification from cDNA? - (reply: 4)
352. What do you use to check primer secondary structure? - (reply: 3)
353. CT of qPCR - (reply: 3)
354. Need help with my real time RT-PCR Plate Set up - (reply: 8)
355. PCR product as template for in vitro transcription - (reply: 1)
356. Sybr Green vs Taqman -- a practical approach - (reply: 2)
357. Problem with 3 step PCR - (reply: 3)
358. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
359. ARMS-PCR - (reply: 1)
360. Scaling up PCR to get more DNA - (reply: 5)