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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
332. analysing qpcr data and plotting standard curve - (reply: 1)
333. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
334. PCR product as standard curve template - (reply: 6)
335. TaqMan CN assay - control sample duplicated - (reply: 1)
336. qPCR - no amplification curve but suitable melting curve - (reply: 3)
337. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
338. Basic question about qPCR and endogenous RNA control. - (reply: 4)
339. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
340. something very basic, why called "event-specific detection" - (reply: 1)
341. skip 65 degrees protocol of Reverse transcription - (reply: 4)
342. Mystery in my PCR - (reply: 5)
343. Whole genome amplification from cDNA? - (reply: 4)
344. What do you use to check primer secondary structure? - (reply: 3)
345. CT of qPCR - (reply: 3)
346. Need help with my real time RT-PCR Plate Set up - (reply: 8)
347. PCR product as template for in vitro transcription - (reply: 1)
348. Sybr Green vs Taqman -- a practical approach - (reply: 2)
349. Problem with 3 step PCR - (reply: 3)
350. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
351. ARMS-PCR - (reply: 1)
352. Scaling up PCR to get more DNA - (reply: 5)
353. RNA concentration suddenly drops - (reply: 3)
354. Problem using evagreen (evagreen vs sybrgreen) - (reply: 4)
355. PCR to get 10kbp product - (reply: 4)
356. site-directed mutagenesis primer Tm - (reply: 4)
357. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
358. RNA concentration to start RT reaction - (reply: 1)
359. Random vs oligo primer in preamplification RT - (reply: 1)
360. Smart Cycler II software and Absolute quantification help - (reply: 1)