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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. Gene sequence for Real time PCR - (reply: 2)
332. qPCR result of pooled samples - (reply: 1)
333. transcript variant X - (reply: 5)
334. RNA extraction for RT-PCR - (reply: 3)
335. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
336. problems with gDNA doing real time PCR in yeast - (reply: 2)
337. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
338. hybridization probe - (reply: 1)
339. How to get rid of bands in PCR negative control - (reply: 10)
340. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
341. standard curve using genomic dna - (reply: 1)
342. PCR problem - (reply: 2)
343. defining ratio of alternative spliced gene with qPCR - (reply: 14)
344. Poor efficiency standard curve - (reply: 2)
345. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
346. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
347. PCR gene specific amplification problem - (reply: 3)
348. Failure SYBRGREEN PCR - (reply: 4)
349. Pfaffl Method for relative - (reply: 4)
350. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
351. experiences with NuPCR from Illumina? - (reply: 1)
352. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
353. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
354. 100 ng RNA for cDNA synthesis - (reply: 4)
355. Inexplicable qPCR failure in single wells - (reply: 12)
356. negative control well 300 bp band - (reply: 3)
357. Reference gene for normalisation - for different growth rates - (reply: 3)
358. Strange amplification plots with high Ct variability - (reply: 1)
359. How big a role does mixing play in PCR - (reply: 1)
360. Melting curve is irregular for primer optimization - (reply: 5)