Protocol Online logo
Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. How big a role does mixing play in PCR - (reply: 1)
332. Melting curve is irregular for primer optimization - (reply: 5)
333. Designing primers for ABO blood groups - (reply: 1)
334. Methylight Results Analysis - (reply: 1)
335. GAPDH Ct value - (reply: 4)
336. dissociation curves - (reply: 1)
337. RT-PCR primer design - (reply: 7)
338. How to amplify very short PCR template - (reply: 4)
339. Correcting for efficiencies and differences among replicate Ct values - (reply: 2)
340. Is this primer okay? - (reply: 4)
341. Dilution calculation - (reply: 3)
342. Common causes for low RNA A260/230 ratios - (reply: 7)
343. Limit of detection for salmon gDNA? - (reply: 7)
344. Intensifying signal from positive control - (reply: 5)
345. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
346. analysing qpcr data and plotting standard curve - (reply: 1)
347. Is "mini-mixing" acceptable/standard practice? - (reply: 4)
348. PCR product as standard curve template - (reply: 6)
349. TaqMan CN assay - control sample duplicated - (reply: 1)
350. qPCR - no amplification curve but suitable melting curve - (reply: 3)
351. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
352. Basic question about qPCR and endogenous RNA control. - (reply: 4)
353. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
354. something very basic, why called "event-specific detection" - (reply: 1)
355. skip 65 degrees protocol of Reverse transcription - (reply: 4)
356. Mystery in my PCR - (reply: 5)
357. Whole genome amplification from cDNA? - (reply: 4)
358. What do you use to check primer secondary structure? - (reply: 3)
359. CT of qPCR - (reply: 3)
360. Need help with my real time RT-PCR Plate Set up - (reply: 8)