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Top : New Forum Archives (2009-): : PCR--RT-PCR-and-Real-Time-PCR
331. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
332. excess rna sample affect qpcr? - (reply: 2)
333. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
334. finding the corrosponding primers - (reply: 2)
335. Gene sequence for Real time PCR - (reply: 2)
336. qPCR result of pooled samples - (reply: 1)
337. transcript variant X - (reply: 5)
338. RNA extraction for RT-PCR - (reply: 3)
339. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
340. problems with gDNA doing real time PCR in yeast - (reply: 2)
341. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
342. hybridization probe - (reply: 1)
343. How to get rid of bands in PCR negative control - (reply: 10)
344. Protocol for qPCR using the ABI SYBRŪ Green PCR Master Mix - (reply: 1)
345. standard curve using genomic dna - (reply: 1)
346. PCR problem - (reply: 2)
347. defining ratio of alternative spliced gene with qPCR - (reply: 14)
348. Poor efficiency standard curve - (reply: 2)
349. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
350. Real time (CT IN NEGETIVE WELL AND DOBLE MELTING CURVE) - (reply: 2)
351. PCR gene specific amplification problem - (reply: 3)
352. Failure SYBRGREEN PCR - (reply: 4)
353. Pfaffl Method for relative - (reply: 4)
354. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
355. experiences with NuPCR from Illumina? - (reply: 1)
356. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
357. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
358. 100 ng RNA for cDNA synthesis - (reply: 4)
359. Inexplicable qPCR failure in single wells - (reply: 12)
360. negative control well 300 bp band - (reply: 3)